(Circular) (Six-base) MAPSORT of: pFN21AE4522.seq Check: 1027 from: 1 to: 6391 pFN21AE4522 6391 bp With 182 enzymes: SgfI * March 28, 2012 14:08 .. AatII G_ACGT'C Cuts at: 278 331 414 600 1536 4201 278 Size: 53 83 186 936 2665 2468 Fragments arranged by size: 2665 2468 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 2499 3734 1937 Size: 562 1235 4594 Fragments arranged by size: 4594 1235 562 Acc65I G'GTAC_C Cuts at: 3712 3712 Size: 6391 AclI AA'CG_TT Cuts at: 1563 4518 4891 1563 Size: 2955 373 3063 Fragments arranged by size: 3063 2955 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 2678 2907 2941 4454 5838 6106 1632 Size: 1046 229 34 1513 1384 268 1917 Fragments arranged by size: 1917 1513 1384 1046 268 229 34 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 6194 AflIII A'CryG_T Cuts at: 1203 5290 6267 1203 Size: 4087 977 1327 Fragments arranged by size: 4087 1327 977 AgeI A'CCGG_T Cuts at: 3146 5222 3146 Size: 2076 4315 AhdI GACnn_n'nnGTC Cuts at: 2352 5120 2352 Size: 2768 3623 AleI CACnn'nnGTG Cuts at: 1166 2174 3162 1166 Size: 1008 988 4395 Fragments arranged by size: 4395 1008 988 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 2770 1184 Size: 1586 4805 AloI GAACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 2180 2212 2528 2560 2180 Size: 32 316 32 6011 Fragments arranged by size: 6011 316 32 32 AlwNI CAG_nnn'CTG Cuts at: 2930 4134 5706 2930 Size: 1204 1572 3615 Fragments arranged by size: 3615 1572 1204 ApaI G_GGCC'C Cuts at: 2372 2372 Size: 6391 ApaLI G'TGCA_C Cuts at: 2622 4448 5604 2622 Size: 1826 1156 3409 Fragments arranged by size: 3409 1826 1156 ApoI r'AATT_y Cuts at: 1493 2393 2442 3094 3271 3700 3926 4078 6187 Size: 900 49 652 177 429 226 152 2109 Cuts at: 6187 1493 Size: 1697 Fragments arranged by size: 2109 1697 900 652 429 226 177 152 49 AseI AT'TA_AT Cuts at: 160 1032 4945 160 Size: 872 3913 1606 Fragments arranged by size: 3913 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 6391 AvaI C'yCGr_G Cuts at: 1870 1943 3716 1870 Size: 73 1773 4545 Fragments arranged by size: 4545 1773 73 BamHI G'GATC_C Cuts at: 3721 4088 3721 Size: 367 6024 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2729 3712 5167 618 Size: 325 217 627 942 983 1455 1842 Fragments arranged by size: 1842 1455 983 942 627 325 217 BanII G_rGCy'C Cuts at: 1391 2372 3710 1391 Size: 981 1338 4072 Fragments arranged by size: 4072 1338 981 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 3499 928 Size: 971 1600 3820 Fragments arranged by size: 3820 1600 971 BbvCI CC'TCA_GC Cuts at: 3309 3309 Size: 6391 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 2050 2084 4582 4616 1903 Size: 34 113 34 2498 34 3678 Fragments arranged by size: 3678 2498 113 34 34 34 BciVI GTATCCnnnnn_n' Cuts at: 2311 4283 5493 2311 Size: 1972 1210 3209 Fragments arranged by size: 3209 1972 1210 BclI T'GATC_A Cuts at: 1548 1548 Size: 6391 BfrBI ATG'CAT Cuts at: 2680 2680 Size: 6391 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 5002 136 Size: 107 122 71 631 605 3330 1525 Fragments arranged by size: 3330 1525 631 605 122 107 71 BglII A'GATC_T Cuts at: 2661 6386 2661 Size: 3725 2666 BlpI GC'TnA_GC Cuts at: 3808 3808 Size: 6391 Bme1580I G_kGCm'C Cuts at: 1790 2060 2372 2626 2734 4452 5608 1790 Size: 270 312 254 108 1718 1156 2573 Fragments arranged by size: 2573 1718 1156 312 270 254 108 BmgBI CAC'GTC Cuts at: 1318 1318 Size: 6391 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 2991 3434 3534 Size: 416 53 83 399 24 1574 443 100 Cuts at: 3534 4122 5080 442 Size: 588 958 1753 Fragments arranged by size: 1753 1574 958 588 443 416 399 100 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 6391 BplI GAGnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 3039 3039 Size: 6391 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2406 2786 5051 1239 Size: 1167 380 2265 2579 Fragments arranged by size: 2579 2265 1167 380 Bpu10I CC'TnA_GC Cuts at: 3309 3309 Size: 6391 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 4516 5381 5679 4516 Size: 865 298 5228 Fragments arranged by size: 5228 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 2819 3059 5054 882 Size: 617 1320 240 1995 2219 Fragments arranged by size: 2219 1995 1320 617 240 BsaAI yAC'GTr Cuts at: 493 1204 4182 493 Size: 711 2978 2702 Fragments arranged by size: 2978 2702 711 BsaBI GATnn'nnATC Cuts at: 2877 6385 2877 Size: 3508 2883 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 4198 4580 5215 275 Size: 53 83 186 936 2665 382 635 1451 Fragments arranged by size: 2665 1451 936 635 382 186 83 53 BsaWI w'CCGG_w Cuts at: 3146 4824 5222 5496 5643 6054 3146 Size: 1678 398 274 147 411 3483 Fragments arranged by size: 3483 1678 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 1178 Size: 30 165 30 6166 Fragments arranged by size: 6166 165 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 3146 3311 1186 Size: 1960 165 4266 Fragments arranged by size: 4266 1960 165 BseYI C'CCAG_C Cuts at: 3645 5594 6090 3645 Size: 1949 496 3946 Fragments arranged by size: 3946 1949 496 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 3657 1163 Size: 573 1921 3897 Fragments arranged by size: 3897 1921 573 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 2194 2257 3841 4602 5630 1540 Size: 275 186 193 63 1584 761 1028 2301 Fragments arranged by size: 2301 1584 1028 761 275 193 186 63 BsiHKAI G_wGCw'C Cuts at: 2626 3025 4452 4537 5608 2626 Size: 399 1427 85 1071 3409 Fragments arranged by size: 3409 1427 1071 399 85 BsmI GAATG_Cn' Cuts at: 2961 3902 3995 2961 Size: 941 93 5357 Fragments arranged by size: 5357 941 93 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2060 2372 2626 2734 3025 3710 4452 Size: 399 270 312 254 108 291 685 742 Cuts at: 4452 4537 5608 1391 Size: 85 1071 2174 Fragments arranged by size: 2174 1071 742 685 399 312 291 270 254 108 85 BspEI T'CCGG_A Cuts at: 6054 6054 Size: 6391 BspHI T'CATG_A Cuts at: 4280 6010 4280 Size: 1730 4661 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 3746 844 Size: 2902 3489 BsrBI CCG'CTC Cuts at: 1394 1823 2064 3519 4278 1394 Size: 429 241 1455 759 3507 Fragments arranged by size: 3507 1455 759 429 241 BsrDI GCAATG_nn' Cuts at: 65 1248 2940 3627 4886 5060 65 Size: 1183 1692 687 1259 174 1396 Fragments arranged by size: 1692 1396 1259 1183 687 174 BsrFI r'CCGG_y Cuts at: 1700 1810 2149 2193 3146 5035 5222 1700 Size: 110 339 44 953 1889 187 2869 Fragments arranged by size: 2869 1889 953 339 187 110 44 BsrGI T'GTAC_A Cuts at: 96 3552 96 Size: 3456 2935 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 6391 BssSI C'ACGA_G Cuts at: 2059 3013 4451 5463 2059 Size: 954 1438 1012 2987 Fragments arranged by size: 2987 1438 1012 954 BstAPI GCAn_nnn'nTGC Cuts at: 794 794 Size: 6391 BstBI TT'CG_AA Cuts at: 5195 5195 Size: 6391 BstEII G'GTnAC_C Cuts at: 1184 1184 Size: 6391 BstXI CCAn_nnnn'nTGG Cuts at: 2176 2981 3078 2176 Size: 805 97 5489 Fragments arranged by size: 5489 805 97 BstYI r'GATC_y Cuts at: 1972 2661 2709 3721 4088 4473 4490 5942 6028 Size: 689 48 1012 367 385 17 1452 86 Cuts at: 6028 6040 6386 1972 Size: 12 346 1977 Fragments arranged by size: 1977 1452 1012 689 385 367 346 86 48 17 12 BtgI C'CryG_G Cuts at: 513 1065 2004 2142 2989 513 Size: 552 939 138 847 3915 Fragments arranged by size: 3915 939 847 552 138 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 2209 2218 487 Size: 683 56 983 9 4660 Fragments arranged by size: 4660 983 683 56 9 BtsI GCAGTG_nn' Cuts at: 3669 3907 4701 4721 3669 Size: 238 794 20 5339 Fragments arranged by size: 5339 794 238 20 ClaI AT'CG_AT Cuts at: 1554 3558 1554 Size: 2004 4387 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 6356 DraI TTT'AAA Cuts at: 3695 3695 Size: 6391 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 2833 3602 5398 809 Size: 507 1517 769 1796 1802 Fragments arranged by size: 1802 1796 1517 769 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2191 3265 3838 Size: 54 1425 50 275 18 361 1074 573 Cuts at: 3838 4727 6356 8 Size: 889 1629 43 Fragments arranged by size: 1629 1425 1074 889 573 361 275 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 2191 3838 1537 Size: 275 379 1647 4090 Fragments arranged by size: 4090 1647 379 275 EarI CTCTTCn'nnn_ Cuts at: 1353 4321 1353 Size: 2968 3423 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 4978 5352 5498 387 Size: 1064 138 3389 374 146 1280 Fragments arranged by size: 3389 1280 1064 374 146 138 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2406 2678 2786 2907 2941 4454 5051 Size: 393 774 272 108 121 34 1513 597 Cuts at: 5051 5838 6106 1239 Size: 787 268 1524 Fragments arranged by size: 1524 1513 787 774 597 393 272 268 121 108 34 EcoO109I rG'GnC_Cy Cuts at: 2368 2369 2414 3343 3595 2368 Size: 1 45 929 252 5164 Fragments arranged by size: 5164 929 252 45 1 EcoRI G'AATT_C Cuts at: 3700 4078 3700 Size: 378 6013 FalI AAGnnnnnCTTnnnnnnnn_nnnnn' Cuts at: 2519 2654 2519 Size: 135 6256 FspI TGC'GCA Cuts at: 4897 4897 Size: 6391 HaeII r_GCGC'y Cuts at: 1678 2103 5538 1678 Size: 425 3435 2531 Fragments arranged by size: 3435 2531 425 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2338 2370 2646 2678 3340 Size: 32 135 32 766 32 276 32 662 Cuts at: 3340 3372 5032 5064 5106 5138 1373 Size: 32 1660 32 42 32 2626 Fragments arranged by size: 2626 1660 766 662 276 135 42 32 32 32 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 2752 3735 3981 669 Size: 988 281 814 983 246 3079 Fragments arranged by size: 3079 988 983 814 281 246 HindIII A'AGCT_T Cuts at: 748 2583 748 Size: 1835 4556 HpaI GTT'AAC Cuts at: 2752 3981 2752 Size: 1229 5162 KpnI G_GTAC'C Cuts at: 3716 3716 Size: 6391 MfeI C'AATT_G Cuts at: 2463 3990 2463 Size: 1527 4864 MluI A'CGCG_T Cuts at: 6267 6267 Size: 6391 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 3535 5505 5689 1305 Size: 2230 1970 184 2007 Fragments arranged by size: 2230 2007 1970 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 10 Size: 54 1425 343 4569 Fragments arranged by size: 4569 1425 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 2174 3162 4349 4708 4867 518 Size: 648 1008 988 1187 359 159 2042 Fragments arranged by size: 2042 1187 1008 988 648 359 159 MspA1I CmG'CkG Cuts at: 1606 2158 2813 2991 3649 3807 4484 5632 5877 Size: 552 655 178 658 158 677 1148 245 Cuts at: 5877 1606 Size: 2120 Fragments arranged by size: 2120 1148 677 658 655 552 245 178 158 NaeI GCC'GGC Cuts at: 2151 2151 Size: 6391 NcoI C'CATG_G Cuts at: 513 1065 2004 513 Size: 552 939 4900 Fragments arranged by size: 4900 939 552 NdeI CA'TA_TG Cuts at: 387 387 Size: 6391 NgoMIV G'CCGG_C Cuts at: 2149 2149 Size: 6391 NheI G'CTAG_C Cuts at: 1052 1052 Size: 6391 NotI GC'GGCC_GC Cuts at: 3838 3838 Size: 6391 NsiI A_TGCA'T Cuts at: 2682 2682 Size: 6391 NspI r_CATG'y Cuts at: 1132 1229 3010 3749 5294 1132 Size: 97 1781 739 1545 2229 Fragments arranged by size: 2229 1781 1545 739 97 PasI CC'CwG_GG Cuts at: 1348 2363 1348 Size: 1015 5376 PciI A'CATG_T Cuts at: 5290 5290 Size: 6391 PflMI CCAn_nnn'nTGG Cuts at: 1108 3446 1108 Size: 2338 4053 PmeI GTTT'AAAC Cuts at: 3695 3695 Size: 6391 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 4460 4492 5973 6005 4460 Size: 32 1481 32 4846 Fragments arranged by size: 4846 1481 32 32 PpuMI rG'GwC_Cy Cuts at: 2414 3595 2414 Size: 1181 5210 PshAI GACnn'nnGTC Cuts at: 1613 2984 1613 Size: 1371 5020 PsiI TTA'TAA Cuts at: 3961 3961 Size: 6391 PspOMI G'GGCC_C Cuts at: 2368 2368 Size: 6391 PstI C_TGCA'G Cuts at: 830 3654 3743 830 Size: 2824 89 3478 Fragments arranged by size: 3478 2824 89 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 6391 PvuII CAG'CTG Cuts at: 2813 3649 2813 Size: 836 5555 SacII CC_GC'GG Cuts at: 2992 2992 Size: 6391 SalI G'TCGA_C Cuts at: 1936 3733 1936 Size: 1797 4594 SbfI CC_TGCA'GG Cuts at: 3743 3743 Size: 6391 ScaI AGT'ACT Cuts at: 4639 6351 4639 Size: 1712 4679 SexAI A'CCwGG_T Cuts at: 2817 3281 2817 Size: 464 5927 SfcI C'TryA_G Cuts at: 826 1045 3650 3739 4874 5555 5746 826 Size: 219 2605 89 1135 681 191 1471 Fragments arranged by size: 2605 1471 1135 681 219 191 89 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 6391 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 6391 SmaI CCC'GGG Cuts at: 1872 3718 1872 Size: 1846 4545 SmlI C'TyrA_G Cuts at: 820 1017 1943 4495 5396 5658 820 Size: 197 926 2552 901 262 1553 Fragments arranged by size: 2552 1553 926 901 262 197 SnaBI TAC'GTA Cuts at: 493 4182 493 Size: 3689 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 6391 SphI G_CATG'C Cuts at: 1132 3010 3749 1132 Size: 1878 739 3774 Fragments arranged by size: 3774 1878 739 SspI AAT'ATT Cuts at: 5 52 2401 4315 5 Size: 47 2349 1914 2081 Fragments arranged by size: 2349 2081 1914 47 StuI AGG'CCT Cuts at: 1517 1517 Size: 6391 StyI C'CwwG_G Cuts at: 513 1065 2004 2503 3830 513 Size: 552 939 499 1327 3074 Fragments arranged by size: 3074 1327 939 552 499 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 2243 3691 4418 4435 4588 4773 5218 Size: 1255 663 1448 727 17 153 185 445 Cuts at: 5218 325 Size: 1498 Fragments arranged by size: 1498 1448 1255 727 663 445 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 3552 4637 6349 Size: 274 80 33 51 1443 1575 1085 1712 Cuts at: 6349 96 Size: 138 Fragments arranged by size: 1712 1575 1443 1085 274 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 6351 6383 6351 Size: 32 6359 Tth111I GACn'n_nGTC Cuts at: 1316 1316 Size: 6391 XbaI T'CTAG_A Cuts at: 3727 3727 Size: 6391 XcmI CCAnnnn_n'nnnnTGG Cuts at: 2176 2176 Size: 6391 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 6391 XmaI C'CCGG_G Cuts at: 1870 3716 1870 Size: 1846 4545 XmnI GAAnn'nnTTC Cuts at: 2087 3275 3377 3454 4520 2087 Size: 1188 102 77 1066 3958 Fragments arranged by size: 3958 1188 1066 102 77 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 4199 276 Size: 53 83 186 936 2665 2468 Fragments arranged by size: 2665 2468 936 186 83 53 Enzymes that do cut: AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AloI AlwNI ApaI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbsI BbvCI BcgI BciVI BclI BfrBI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BplI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI EarI EciI Eco57MI EcoO109I EcoRI FalI FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I NaeI NcoI NdeI NgoMIV NheI NotI NsiI NspI PasI PciI PflMI PmeI PpiI PpuMI PshAI PsiI PspOMI PstI PvuI PvuII SacII SalI SbfI ScaI SexAI SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI Enzymes that do not cut: AarI AfeI AscI AvrII BaeI BbeI BsiWI BsmBI BstZ17I Bsu36I DraIII EcoICRI EcoNI EcoRV FseI FspAI KasI NarI NruI PacI PfoI PmlI PspXI PsrI RsrII SacI SanDI SapI SfiI SfoI SrfI SwaI