################################## # NO CUT ENZYMES ( 116 enzymes) # ################################## AatII Acc65I AclI AflII AflIII AgeI AhdI Alw44I AlwNI AocI Aor51HI ApaLI AscI AseI AsnI Asp700I Asp718I AspEI AviII AvrII BamHI BanI BbeI BbrPI BbvCI BcgI BciVI BfrI BlnI BlpI Bpu1102I BsaAI BsaBI BsgI BsiEI BsiWI BsmFI BsmI Bsp1407I BspDI BspLU11I BsrFI BsrGI BssHII BstAPI BstBI BstEII BstPI Bsu36I CelII Cfr10I ClaI CpoI DrdI DsaI EagI Eam1105I EclXI Eco47III Eco52I Eco81I EcoNI EcoO65I EcoRI EcoT22I FseI FspI HindIII HpaI KasI KpnI KspI MamI MfeI MluI MunI NaeI NarI NcoI NdeI NgoMIV NheI NotI NruI NsiI NspV PacI PinAI PmaCI PmeI PmlI PshAI PshBI Psp1406I PstI PvuI RsrII SacII SbfI SexAI SfoI SfuI SgrAI SmaI SnaBI SpeI SphI Sse8387I SspBI SspI StuI SwaI TfiI XmaCI XmaI XmnI ################################### # ONE CUT ENZYMES ( 42 enzymes) # ################################### AccIII 1541 TCCGGA T^CCGGA ApaI 1288 GGGCCC GGGCC^C AspI 1137 GACACTGTC GAC[ACGT]^[ACGT][ACGT]GTC BalI 14 TGGCCA TGG^CCA BclI 930 TGATCA T^GATCA BcnI 1058 CCCGG CC^[GC]GG BglII 1425 AGATCT A^GATCT BsaWI 1541 TCCGGA [AT]^CCGG[AT] BseAI 1541 TCCGGA T^CCGGA BsmBI 840 CGTCTC CGTCTC(1/5) BspEI 1541 TCCGGA T^CCGGA BspHI 540 TCATGA T^CATGA BsrDI 1023 CATTGC GCAATG(2/0) BssSI 1271 CACGAG CACGAG(-5/-1) Bst1107I 1212 GTATAC GTA^TAC BstZ17I 1212 GTATAC GTA^TAC DraI 354 TTTAAA TTT^AAA EaeI 14 TGGCCA [CT]^GGCC[GA] EcoRV 1642 GATATC GAT^ATC EcoT14I 170 CCAAGG C^C[AT][AT]GG FauI 504 GCGGG CCCGC(4/6) FbaI 930 TGATCA T^GATCA HaeII 581 AGCGCC [GA]GCGC^[CT] MaeII 1865 ACGT A^CGT MluNI 14 TGGCCA TGG^CCA MroI 1541 TCCGGA T^CCGGA MscI 14 TGGCCA TGG^CCA NciI 1058 CCCGG CC^[GC]GG PflFI 1137 GACACTGTC GAC[ACGT]^[ACGT][ACGT]GTC PflMI 1156 CCACCATGTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG PpuMI 1855 AGGACCC [GA]G^G[AT]CC[CT] PvuII 1414 CAGCTG CAG^CTG RcaI 540 TCATGA T^CATGA SacI 571 GAGCTC GAGCT^C SalI 493 GTCGAC G^TCGAC SapI 1611 GCTCTTC GCTCTTC(1/4) ScaI 247 AGTACT AGT^ACT SfcI 1087 CTACAG C^T[GA][CT]AG StyI 170 CCAAGG C^C[AT][AT]GG Tth111I 1137 GACACTGTC GAC[ACGT]^[ACGT][ACGT]GTC Van91I 1156 CCACCATGTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG XbaI 1245 TCTAGA T^CTAGA ################################### # TWO CUT ENZYMES ( 28 enzymes) # ################################### AccI 493 GTCGAC GT^[AC][GT]AC 1212 GTATAC AccII 626 CGCG CG^CG 947 CGCG AcsI 1249 GAATTT [GA]^AATT[CT] 1745 AAATTT AcyI 223 GGCGTC G[GA]^CG[CT]C 1657 GACGCC ApoI 1249 GAATTT [GA]^AATT[CT] 1745 AAATTT AvaI 209 CTCGAG C^[CT]CG[GA]G 443 CTCGAG BbsI 942 GAAGAC GAAGAC(2/6) 1080 GAAGAC BpuAI 942 GAAGAC GAAGAC(2/6) 1080 GAAGAC BsaHI 223 GGCGTC G[GA]^CG[CT]C 1657 GACGCC BsoBI 209 CTCGAG C^[CT]CG[GA]G 443 CTCGAG BspMI 654 ACCTGC ACCTGC(4/8) 1731 GCAGGT BsrBI 502 GAGCGG CCGCTC(-3/-3) 529 GAGCGG BstUI 626 CGCG CG^CG 947 CGCG HapII 1059 CCGG C^CGG 1542 CCGG Hin1I 223 GGCGTC G[GA]^CG[CT]C 1657 GACGCC HincII 493 GTCGAC GT[CT]^[GA]AC 1308 GTCAAC HindII 493 GTCGAC GT[CT]^[GA]AC 1308 GTCAAC HinfI 441 GACTC G^A[ACGT]TC 1721 GACTC HpaII 1059 CCGG C^CGG 1542 CCGG MspA1I 61 CAGCGG C[AC]G^C[GT]G 1414 CAGCTG MspI 1059 CCGG C^CGG 1542 CCGG MvnI 626 CGCG CG^CG 947 CGCG NspI 890 ACATGC [GA]CATG^[CT] 1607 ACATGC PaeR7I 209 CTCGAG C^TCGAG 443 CTCGAG PleI 441 GACTC GAGTC(4/5) 1721 GACTC SfiI 1056 GGCCCGGCAGGCC GGCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGCC 1581 GGCCTGGCTGGCC XcmI 1628 CCACCAATCACCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG 1652 CCATCGACGCCCTGG XhoI 209 CTCGAG C^TCGAG 443 CTCGAG ######################################### # 3 OR MORE CUT ENZYMES ( 72 enzymes) # ######################################### AciI AfaI AluI AlwI AspHI AvaII BanII BbvI BfaI BglI BmyI BpmI BsaI BsaJI BseRI BsiHKAI BsiYI BslI BsmAI Bsp1286I BsrI BssKI BstF5I BstNI BstXI BstYI Cac8I CfoI Cfr13I DdeI DpnII DraII DraIII EarI EcoO109I EcoRII Fnu4HI FokI HaeIII HgaI HhaI HinP1I HphI ItaI Ksp632I MaeI MaeIII MboI MboII MflI MnlI MseI MslI MvaI MwoI NdeII NlaIII NlaIV RsaI Sau3AI Sau96I ScrFI SfaNI SmlI TaqI Tru9I TseI Tsp45I Tsp509I TspRI TthHB8I XhoII