(Circular) (Six-base) MAPSORT of: pFN21AE6418.seq Check: 6025 from: 1 to: 6574 pFN21AE6418 6574 bp With 182 enzymes: SgfI * March 18, 2015 14:51 .. AarI CACCTGCnnnn'nnnn_ Cuts at: 2668 2668 Size: 6574 AatII G_ACGT'C Cuts at: 278 331 414 600 1536 4384 278 Size: 53 83 186 936 2848 2468 Fragments arranged by size: 2848 2468 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 2499 3218 3917 1937 Size: 562 719 699 4594 Fragments arranged by size: 4594 719 699 562 Acc65I G'GTAC_C Cuts at: 3895 3895 Size: 6574 AclI AA'CG_TT Cuts at: 1563 4701 5074 1563 Size: 3138 373 3063 Fragments arranged by size: 3138 3063 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 2674 2933 3344 3706 4637 6021 6289 1632 Size: 1042 259 411 362 931 1384 268 1917 Fragments arranged by size: 1917 1384 1042 931 411 362 268 259 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 6377 AflIII A'CryG_T Cuts at: 1203 5473 6450 1203 Size: 4270 977 1327 Fragments arranged by size: 4270 1327 977 AgeI A'CCGG_T Cuts at: 5405 5405 Size: 6574 AhdI GACnn_n'nnGTC Cuts at: 5303 5303 Size: 6574 AleI CACnn'nnGTG Cuts at: 1166 3278 1166 Size: 2112 4462 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 1184 Size: 6574 AlwNI CAG_nnn'CTG Cuts at: 4317 5889 4317 Size: 1572 5002 ApaI G_GGCC'C Cuts at: 3297 3297 Size: 6574 ApaLI G'TGCA_C Cuts at: 4631 5787 4631 Size: 1156 5418 ApoI r'AATT_y Cuts at: 1493 3254 3750 3883 4109 4261 6370 1493 Size: 1761 496 133 226 152 2109 1697 Fragments arranged by size: 2109 1761 1697 496 226 152 133 AseI AT'TA_AT Cuts at: 160 1032 5128 160 Size: 872 4096 1606 Fragments arranged by size: 4096 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 6574 AvaI C'yCGr_G Cuts at: 1870 1943 2214 2448 3899 1870 Size: 73 271 234 1451 4545 Fragments arranged by size: 4545 1451 271 234 73 BamHI G'GATC_C Cuts at: 3904 4271 3904 Size: 367 6207 BanI G'GyrC_C Cuts at: 618 943 1160 1787 3895 5350 618 Size: 325 217 627 2108 1455 1842 Fragments arranged by size: 2108 1842 1455 627 325 217 BanII G_rGCy'C Cuts at: 1391 2580 2731 3297 3542 3846 3893 1391 Size: 1189 151 566 245 304 47 4072 Fragments arranged by size: 4072 1189 566 304 245 151 47 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 2954 3092 928 Size: 971 1055 138 4410 Fragments arranged by size: 4410 1055 971 138 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 1987 2021 4765 4799 1903 Size: 34 50 34 2744 34 3678 Fragments arranged by size: 3678 2744 50 34 34 34 BciVI GTATCCnnnnn_n' Cuts at: 4466 5676 4466 Size: 1210 5364 BclI T'GATC_A Cuts at: 1548 2935 1548 Size: 1387 5187 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 2612 3059 3068 Size: 107 122 71 631 605 940 447 9 Cuts at: 3068 3593 5185 136 Size: 525 1592 1525 Fragments arranged by size: 1592 1525 940 631 605 525 447 122 107 71 9 BglII A'GATC_T Cuts at: 3430 6569 3430 Size: 3139 3435 BlpI GC'TnA_GC Cuts at: 3991 3991 Size: 6574 Bme1580I G_kGCm'C Cuts at: 1790 2415 3297 4635 5791 1790 Size: 625 882 1338 1156 2573 Fragments arranged by size: 2573 1338 1156 882 625 BmgBI CAC'GTC Cuts at: 1318 1318 Size: 6574 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 2657 3001 3531 Size: 416 53 83 399 24 1240 344 530 Cuts at: 3531 4305 5263 442 Size: 774 958 1753 Fragments arranged by size: 1753 1240 958 774 530 416 399 344 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 6574 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2540 2612 2722 2984 3357 3810 5234 1239 Size: 1301 72 110 262 373 453 1424 2579 Fragments arranged by size: 2579 1424 1301 453 373 262 110 72 Bpu10I CC'TnA_GC Cuts at: 2421 2421 Size: 6574 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 3803 4699 5564 5862 3803 Size: 896 865 298 4515 Fragments arranged by size: 4515 896 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 2144 3521 3647 5237 882 Size: 617 645 1377 126 1590 2219 Fragments arranged by size: 2219 1590 1377 645 617 126 BsaAI yAC'GTr Cuts at: 493 1204 4365 493 Size: 711 3161 2702 Fragments arranged by size: 3161 2702 711 BsaBI GATnn'nnATC Cuts at: 6568 6568 Size: 6574 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 2229 3663 4381 4763 Size: 53 83 186 936 696 1434 718 382 Cuts at: 4763 5398 275 Size: 635 1451 Fragments arranged by size: 1451 1434 936 718 696 635 382 186 83 53 BsaWI w'CCGG_w Cuts at: 3546 5007 5405 5679 5826 6237 3546 Size: 1461 398 274 147 411 3883 Fragments arranged by size: 3883 1461 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 2088 2118 1178 Size: 30 165 30 685 30 5634 Fragments arranged by size: 5634 685 165 30 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2609 2981 3854 1186 Size: 1423 372 873 3906 Fragments arranged by size: 3906 1423 873 372 BseYI C'CCAG_C Cuts at: 3846 5777 6273 3846 Size: 1931 496 4147 Fragments arranged by size: 4147 1931 496 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 1163 Size: 573 6001 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 4024 4785 5813 1540 Size: 275 186 2023 761 1028 2301 Fragments arranged by size: 2301 2023 1028 761 275 186 BsiHKAI G_wGCw'C Cuts at: 2427 2580 3816 4635 4720 5791 2427 Size: 153 1236 819 85 1071 3210 Fragments arranged by size: 3210 1236 1071 819 153 85 BsmI GAATG_Cn' Cuts at: 4085 4178 4085 Size: 93 6481 BsmBI CGTCTCn'nnnn_ Cuts at: 2851 2851 Size: 6574 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2415 2427 2580 2731 3297 3542 3816 Size: 399 625 12 153 151 566 245 274 Cuts at: 3816 3846 3893 4635 4720 5791 1391 Size: 30 47 742 85 1071 2174 Fragments arranged by size: 2174 1071 742 625 566 399 274 245 153 151 85 47 30 12 BspEI T'CCGG_A Cuts at: 3546 6237 3546 Size: 2691 3883 BspHI T'CATG_A Cuts at: 2545 4463 6193 2545 Size: 1918 1730 2926 Fragments arranged by size: 2926 1918 1730 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 2668 3727 3929 844 Size: 1824 1059 202 3489 Fragments arranged by size: 3489 1824 1059 202 BsrBI CCG'CTC Cuts at: 1394 1823 2509 2536 4461 1394 Size: 429 686 27 1925 3507 Fragments arranged by size: 3507 1925 686 429 27 BsrDI GCAATG_nn' Cuts at: 65 1248 3027 5069 5243 65 Size: 1183 1779 2042 174 1396 Fragments arranged by size: 2042 1779 1396 1183 174 BsrFI r'CCGG_y Cuts at: 1700 1810 5218 5405 1700 Size: 110 3408 187 2869 Fragments arranged by size: 3408 2869 187 110 BsrGI T'GTAC_A Cuts at: 96 96 Size: 6574 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 6574 BssSI C'ACGA_G Cuts at: 3276 4634 5646 3276 Size: 1358 1012 4204 Fragments arranged by size: 4204 1358 1012 BstAPI GCAn_nnn'nTGC Cuts at: 794 794 Size: 6574 BstBI TT'CG_AA Cuts at: 5378 5378 Size: 6574 BstEII G'GTnAC_C Cuts at: 1184 1184 Size: 6574 BstXI CCAn_nnnn'nTGG Cuts at: 2779 3643 3832 2779 Size: 864 189 5521 Fragments arranged by size: 5521 864 189 BstYI r'GATC_y Cuts at: 1972 2634 3174 3430 3904 4271 4656 4673 6125 Size: 662 540 256 474 367 385 17 1452 Cuts at: 6125 6211 6223 6569 1972 Size: 86 12 346 1977 Fragments arranged by size: 1977 1452 662 540 474 385 367 346 256 86 17 12 BstZ17I GTA'TAC Cuts at: 3219 3219 Size: 6574 BtgI C'CryG_G Cuts at: 513 1065 513 Size: 552 6022 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 487 Size: 683 56 5835 Fragments arranged by size: 5835 683 56 BtsI GCAGTG_nn' Cuts at: 3055 4090 4884 4904 3055 Size: 1035 794 20 4725 Fragments arranged by size: 4725 1035 794 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 6574 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 6539 DraI TTT'AAA Cuts at: 2361 3878 2361 Size: 1517 5057 DraIII CAC_nnn'GTG Cuts at: 3167 3783 3871 3167 Size: 616 88 5870 Fragments arranged by size: 5870 616 88 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 5581 809 Size: 507 4265 1802 Fragments arranged by size: 4265 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2019 4021 4910 Size: 54 1425 50 275 18 189 2002 889 Cuts at: 4910 6539 8 Size: 1629 43 Fragments arranged by size: 2002 1629 1425 889 275 189 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 4021 1537 Size: 275 2209 4090 Fragments arranged by size: 4090 2209 275 EarI CTCTTCn'nnn_ Cuts at: 1353 2975 3324 3623 4504 1353 Size: 1622 349 299 881 3423 Fragments arranged by size: 3423 1622 881 349 299 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 5161 5535 5681 387 Size: 1064 138 3572 374 146 1280 Fragments arranged by size: 3572 1280 1064 374 146 138 EcoICRI GAG'CTC Cuts at: 2578 2578 Size: 6574 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2540 2612 2674 2722 2933 2984 3344 Size: 393 908 72 62 48 211 51 360 Cuts at: 3344 3357 3706 3810 4637 5234 6021 6289 1239 Size: 13 349 104 827 597 787 268 1524 Fragments arranged by size: 1524 908 827 787 597 393 360 349 268 211 104 72 62 51 48 13 EcoO109I rG'GnC_Cy Cuts at: 2625 3293 3861 2625 Size: 668 568 5338 Fragments arranged by size: 5338 668 568 EcoRI G'AATT_C Cuts at: 3883 4261 3883 Size: 378 6196 EcoRV GAT'ATC Cuts at: 3649 3649 Size: 6574 FalI AAGnnnnnCTTnnnnnnnn_nnnnn' Cuts at: 2494 2851 2494 Size: 357 6217 FspI TGC'GCA Cuts at: 5080 5080 Size: 6574 HaeII r_GCGC'y Cuts at: 1678 2590 5721 1678 Size: 912 3131 2531 Fragments arranged by size: 3131 2531 912 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2088 2120 3025 3057 3501 Size: 32 135 32 516 32 905 32 444 Cuts at: 3501 3533 5215 5247 5289 5321 1373 Size: 32 1682 32 42 32 2626 Fragments arranged by size: 2626 1682 905 516 444 135 42 32 32 32 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 2500 3315 3918 4164 669 Size: 988 281 562 815 603 246 3079 Fragments arranged by size: 3079 988 815 603 562 281 246 HindIII A'AGCT_T Cuts at: 748 748 Size: 6574 HpaI GTT'AAC Cuts at: 4164 4164 Size: 6574 KpnI G_GTAC'C Cuts at: 3899 3899 Size: 6574 MfeI C'AATT_G Cuts at: 4173 4173 Size: 6574 MluI A'CGCG_T Cuts at: 6450 6450 Size: 6574 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 2037 2784 5688 5872 1305 Size: 732 747 2904 184 2007 Fragments arranged by size: 2904 2007 747 732 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 2021 10 Size: 54 1425 343 189 4563 Fragments arranged by size: 4563 1425 343 189 54 MslI CAynn'nnrTG Cuts at: 518 1166 2550 2894 3048 3194 3278 3378 4532 Size: 648 1384 344 154 146 84 100 1154 Cuts at: 4532 4891 5050 518 Size: 359 159 2042 Fragments arranged by size: 2042 1384 1154 648 359 344 159 154 146 100 84 MspA1I CmG'CkG Cuts at: 1606 2068 3421 3990 4667 5815 6060 1606 Size: 462 1353 569 677 1148 245 2120 Fragments arranged by size: 2120 1353 1148 677 569 462 245 NcoI C'CATG_G Cuts at: 513 1065 513 Size: 552 6022 NdeI CA'TA_TG Cuts at: 387 387 Size: 6574 NheI G'CTAG_C Cuts at: 1052 1052 Size: 6574 NotI GC'GGCC_GC Cuts at: 4021 4021 Size: 6574 NspI r_CATG'y Cuts at: 1132 1229 2899 3616 3932 5477 1132 Size: 97 1670 717 316 1545 2229 Fragments arranged by size: 2229 1670 1545 717 316 97 PasI CC'CwG_GG Cuts at: 1348 1348 Size: 6574 PciI A'CATG_T Cuts at: 5473 5473 Size: 6574 PflMI CCAn_nnn'nTGG Cuts at: 1108 3167 3871 1108 Size: 2059 704 3811 Fragments arranged by size: 3811 2059 704 PfoI T'CCnGG_A Cuts at: 2757 3621 2757 Size: 864 5710 PmeI GTTT'AAAC Cuts at: 3878 3878 Size: 6574 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 4643 4675 6156 6188 4643 Size: 32 1481 32 5029 Fragments arranged by size: 5029 1481 32 32 PpuMI rG'GwC_Cy Cuts at: 3861 3861 Size: 6574 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 6574 PsiI TTA'TAA Cuts at: 4144 4144 Size: 6574 PspOMI G'GGCC_C Cuts at: 3293 3293 Size: 6574 PspXI vC'TCGA_Gb Cuts at: 2214 2214 Size: 6574 PstI C_TGCA'G Cuts at: 830 3926 830 Size: 3096 3478 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 6574 PvuII CAG'CTG Cuts at: 3421 3421 Size: 6574 SacI G_AGCT'C Cuts at: 2580 2580 Size: 6574 SalI G'TCGA_C Cuts at: 1936 2498 3916 1936 Size: 562 1418 4594 Fragments arranged by size: 4594 1418 562 SapI GCTCTTCn'nnn_ Cuts at: 3623 3623 Size: 6574 SbfI CC_TGCA'GG Cuts at: 3926 3926 Size: 6574 ScaI AGT'ACT Cuts at: 2254 4822 6534 2254 Size: 2568 1712 2294 Fragments arranged by size: 2568 2294 1712 SfcI C'TryA_G Cuts at: 826 1045 3092 3922 5057 5738 5929 826 Size: 219 2047 830 1135 681 191 1471 Fragments arranged by size: 2047 1471 1135 830 681 219 191 SfiI GGCCn_nnn'nGGCC Cuts at: 3068 3593 3068 Size: 525 6049 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 6574 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 6574 SmaI CCC'GGG Cuts at: 1872 3901 1872 Size: 2029 4545 SmlI C'TyrA_G Cuts at: 820 1017 1943 2214 2448 3818 4678 5579 5841 Size: 197 926 271 234 1370 860 901 262 Cuts at: 5841 820 Size: 1553 Fragments arranged by size: 1553 1370 926 901 860 271 262 234 197 SnaBI TAC'GTA Cuts at: 493 4365 493 Size: 3872 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 6574 SphI G_CATG'C Cuts at: 1132 3932 1132 Size: 2800 3774 SspI AAT'ATT Cuts at: 5 52 4498 5 Size: 47 4446 2081 Fragments arranged by size: 4446 2081 47 StuI AGG'CCT Cuts at: 1517 1517 Size: 6574 StyI C'CwwG_G Cuts at: 513 1065 2175 4013 513 Size: 552 1110 1838 3074 Fragments arranged by size: 3074 1838 1110 552 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 4601 4618 4771 4956 5401 325 Size: 1255 3021 17 153 185 445 1498 Fragments arranged by size: 3021 1498 1255 445 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 2252 3447 4820 Size: 274 80 33 51 1443 275 1195 1373 Cuts at: 4820 6532 96 Size: 1712 138 Fragments arranged by size: 1712 1443 1373 1195 275 274 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 2543 2575 3806 3838 6534 6566 2543 Size: 32 1231 32 2696 32 2551 Fragments arranged by size: 2696 2551 1231 32 32 32 Tth111I GACn'n_nGTC Cuts at: 1316 3145 1316 Size: 1829 4745 XbaI T'CTAG_A Cuts at: 3250 3910 3250 Size: 660 5914 XcmI CCAnnnn_n'nnnnTGG Cuts at: 3640 3664 3640 Size: 24 6550 XhoI C'TCGA_G Cuts at: 1943 2214 2448 1943 Size: 271 234 6069 Fragments arranged by size: 6069 271 234 XmaI C'CCGG_G Cuts at: 1870 3899 1870 Size: 2029 4545 XmnI GAAnn'nnTTC Cuts at: 4703 4703 Size: 6574 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 4382 276 Size: 53 83 186 936 2848 2468 Fragments arranged by size: 2848 2468 936 186 83 53 Enzymes that do cut: AarI AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbsI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI BsmBI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI BstZ17I BtgI BtgZI BtsI ClaI CspCI DraI DraIII DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoO109I EcoRI EcoRV FalI FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I NcoI NdeI NheI NotI NspI PasI PciI PflMI PfoI PmeI PpiI PpuMI PshAI PsiI PspOMI PspXI PstI PvuI PvuII SacI SalI SapI SbfI ScaI SfcI SfiI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI Enzymes that do not cut: AfeI AloI AscI AvrII BaeI BbeI BbvCI BfrBI BplI BsiWI Bsu36I EcoNI FseI FspAI KasI NaeI NarI NgoMIV NruI NsiI PacI PmlI PsrI RsrII SacII SanDI SexAI SfoI SrfI SwaI