(Linear) (Six-base) MAPSORT of: KIEE6418.seq Check: 3436 from: 1 to: 1869 KIEE6418.orf With 182 enzymes: SgfI * March 16, 2015 11:31 .. AarI CACCTGCnnnn'nnnn_ Cuts at: 0 663 1869 Size: 663 1206 AccI GT'mk_AC Cuts at: 0 494 1213 1869 Size: 494 719 656 Fragments arranged by size: 719 656 494 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 0 669 928 1339 1701 1869 Size: 669 259 411 362 168 Fragments arranged by size: 669 411 362 259 168 AleI CACnn'nnGTG Cuts at: 0 1273 1869 Size: 1273 596 ApaI G_GGCC'C Cuts at: 0 1292 1869 Size: 1292 577 ApoI r'AATT_y Cuts at: 0 1249 1745 1869 Size: 1249 496 124 Fragments arranged by size: 1249 496 124 AvaI C'yCGr_G Cuts at: 0 209 443 1869 Size: 209 234 1426 Fragments arranged by size: 1426 234 209 BanII G_rGCy'C Cuts at: 0 575 726 1292 1537 1841 1869 Size: 575 151 566 245 304 28 Fragments arranged by size: 575 566 304 245 151 28 BbsI GAAGACnn'nnnn_ Cuts at: 0 949 1087 1869 Size: 949 138 782 Fragments arranged by size: 949 782 138 BclI T'GATC_A Cuts at: 0 930 1869 Size: 930 939 BglI GCCn_nnn'nGGC Cuts at: 0 607 1054 1063 1588 1869 Size: 607 447 9 525 281 Fragments arranged by size: 607 525 447 281 9 BglII A'GATC_T Cuts at: 0 1425 1869 Size: 1425 444 Bme1580I G_kGCm'C Cuts at: 0 410 1292 1869 Size: 410 882 577 Fragments arranged by size: 882 577 410 BmrI ACTGGGnnnn_n' Cuts at: 0 652 996 1526 1869 Size: 652 344 530 343 Fragments arranged by size: 652 530 344 343 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 0 535 607 717 979 1352 1805 1869 Size: 535 72 110 262 373 453 64 Fragments arranged by size: 535 453 373 262 110 72 64 Bpu10I CC'TnA_GC Cuts at: 0 416 1869 Size: 416 1453 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 0 1798 1869 Size: 1798 71 BsaI GGTCTCn'nnnn_ Cuts at: 0 139 1516 1642 1869 Size: 139 1377 126 227 Fragments arranged by size: 1377 227 139 126 BsaHI Gr'CG_yC Cuts at: 0 224 1658 1869 Size: 224 1434 211 Fragments arranged by size: 1434 224 211 BsaWI w'CCGG_w Cuts at: 0 1541 1869 Size: 1541 328 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 0 83 113 1869 Size: 83 30 1756 Fragments arranged by size: 1756 83 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 0 604 976 1849 1869 Size: 604 372 873 20 Fragments arranged by size: 873 604 372 20 BseYI C'CCAG_C Cuts at: 0 1841 1869 Size: 1841 28 BsiHKAI G_wGCw'C Cuts at: 0 422 575 1811 1869 Size: 422 153 1236 58 Fragments arranged by size: 1236 422 153 58 BsmBI CGTCTCn'nnnn_ Cuts at: 0 846 1869 Size: 846 1023 Bsp1286I G_dGCh'C Cuts at: 0 410 422 575 726 1292 1537 1811 1841 Size: 410 12 153 151 566 245 274 30 Cuts at: 1841 1869 Size: 28 Fragments arranged by size: 566 410 274 245 153 151 30 28 12 BspEI T'CCGG_A Cuts at: 0 1541 1869 Size: 1541 328 BspHI T'CATG_A Cuts at: 0 540 1869 Size: 540 1329 BspMI ACCTGCnnnn'nnnn_ Cuts at: 0 663 1722 1869 Size: 663 1059 147 Fragments arranged by size: 1059 663 147 BsrBI CCG'CTC Cuts at: 0 504 531 1869 Size: 504 27 1338 Fragments arranged by size: 1338 504 27 BsrDI GCAATG_nn' Cuts at: 0 1022 1869 Size: 1022 847 BssSI C'ACGA_G Cuts at: 0 1271 1869 Size: 1271 598 BstXI CCAn_nnnn'nTGG Cuts at: 0 774 1638 1827 1869 Size: 774 864 189 42 Fragments arranged by size: 864 774 189 42 BstYI r'GATC_y Cuts at: 0 629 1169 1425 1869 Size: 629 540 256 444 Fragments arranged by size: 629 540 444 256 BstZ17I GTA'TAC Cuts at: 0 1214 1869 Size: 1214 655 BtsI GCAGTG_nn' Cuts at: 0 1050 1869 Size: 1050 819 DraI TTT'AAA Cuts at: 0 356 1869 Size: 356 1513 DraIII CAC_nnn'GTG Cuts at: 0 1162 1778 1866 1869 Size: 1162 616 88 3 Fragments arranged by size: 1162 616 88 3 EaeI y'GGCC_r Cuts at: 0 14 1869 Size: 14 1855 EarI CTCTTCn'nnn_ Cuts at: 0 970 1319 1618 1869 Size: 970 349 299 251 Fragments arranged by size: 970 349 299 251 EcoICRI GAG'CTC Cuts at: 0 573 1869 Size: 573 1296 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 0 535 607 669 717 928 979 1339 1352 Size: 535 72 62 48 211 51 360 13 Cuts at: 1352 1701 1805 1869 Size: 349 104 64 Fragments arranged by size: 535 360 349 211 104 72 64 62 51 48 13 EcoO109I rG'GnC_Cy Cuts at: 0 620 1288 1856 1869 Size: 620 668 568 13 Fragments arranged by size: 668 620 568 13 EcoRV GAT'ATC Cuts at: 0 1644 1869 Size: 1644 225 FalI AAGnnnnnCTTnnnnnnnn_nnnnn' Cuts at: 0 489 846 1869 Size: 489 357 1023 Fragments arranged by size: 1023 489 357 HaeII r_GCGC'y Cuts at: 0 585 1869 Size: 585 1284 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 0 83 115 1020 1052 1496 1528 1869 Size: 83 32 905 32 444 32 341 Fragments arranged by size: 905 444 341 83 32 32 32 HincII GTy'rAC Cuts at: 0 495 1310 1869 Size: 495 815 559 Fragments arranged by size: 815 559 495 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 0 32 779 1869 Size: 32 747 1090 Fragments arranged by size: 1090 747 32 MscI TGG'CCA Cuts at: 0 16 1869 Size: 16 1853 MslI CAynn'nnrTG Cuts at: 0 545 889 1043 1189 1273 1373 1869 Size: 545 344 154 146 84 100 496 Fragments arranged by size: 545 496 344 154 146 100 84 MspA1I CmG'CkG Cuts at: 0 63 1416 1869 Size: 63 1353 453 Fragments arranged by size: 1353 453 63 NspI r_CATG'y Cuts at: 0 894 1611 1869 Size: 894 717 258 Fragments arranged by size: 894 717 258 PflMI CCAn_nnn'nTGG Cuts at: 0 1162 1869 Size: 1162 707 PfoI T'CCnGG_A Cuts at: 0 752 1616 1869 Size: 752 864 253 Fragments arranged by size: 864 752 253 PpuMI rG'GwC_Cy Cuts at: 0 1856 1869 Size: 1856 13 PspOMI G'GGCC_C Cuts at: 0 1288 1869 Size: 1288 581 PspXI vC'TCGA_Gb Cuts at: 0 209 1869 Size: 209 1660 PvuII CAG'CTG Cuts at: 0 1416 1869 Size: 1416 453 SacI G_AGCT'C Cuts at: 0 575 1869 Size: 575 1294 SalI G'TCGA_C Cuts at: 0 493 1869 Size: 493 1376 SapI GCTCTTCn'nnn_ Cuts at: 0 1618 1869 Size: 1618 251 ScaI AGT'ACT Cuts at: 0 249 1869 Size: 249 1620 SfcI C'TryA_G Cuts at: 0 1087 1869 Size: 1087 782 SfiI GGCCn_nnn'nGGCC Cuts at: 0 1063 1588 1869 Size: 1063 525 281 Fragments arranged by size: 1063 525 281 SmlI C'TyrA_G Cuts at: 0 209 443 1813 1869 Size: 209 234 1370 56 Fragments arranged by size: 1370 234 209 56 StyI C'CwwG_G Cuts at: 0 170 1869 Size: 170 1699 TatI w'GTAC_w Cuts at: 0 247 1442 1869 Size: 247 1195 427 Fragments arranged by size: 1195 427 247 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 0 538 570 1801 1833 1869 Size: 538 32 1231 32 36 Fragments arranged by size: 1231 538 36 32 32 Tth111I GACn'n_nGTC Cuts at: 0 1140 1869 Size: 1140 729 XbaI T'CTAG_A Cuts at: 0 1245 1869 Size: 1245 624 XcmI CCAnnnn_n'nnnnTGG Cuts at: 0 1635 1659 1869 Size: 1635 24 210 Fragments arranged by size: 1635 210 24 XhoI C'TCGA_G Cuts at: 0 209 443 1869 Size: 209 234 1426 Fragments arranged by size: 1426 234 209 Enzymes that do cut: AarI AccI AcuI AleI ApaI ApoI AvaI BanII BbsI BclI BglI BglII Bme1580I BmrI BpmI Bpu10I BpuEI BsaI BsaHI BsaWI BsaXI BseRI BseYI BsiHKAI BsmBI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BssSI BstXI BstYI BstZ17I BtsI DraI DraIII EaeI EarI EcoICRI Eco57MI EcoO109I EcoRV FalI HaeII Hin4I HincII MmeI MscI MslI MspA1I NspI PflMI PfoI PpuMI PspOMI PspXI PvuII SacI SalI SapI ScaI SfcI SfiI SmlI StyI TatI TstI Tth111I XbaI XcmI XhoI Enzymes that do not cut: AatII Acc65I AclI AfeI AflII AflIII AgeI AhdI AlfI AloI AlwNI ApaLI AscI AseI AsiSI AvrII BaeI BamHI BanI BbeI BbvCI BcgI BciVI BfrBI BlpI BmgBI BmtI BplI BsaAI BsaBI BsgI BsiEI BsiWI BsmI BsrFI BsrGI BssHII BstAPI BstBI BstEII Bsu36I BtgI BtgZI ClaI CspCI DrdI EagI EciI EcoNI EcoRI FseI FspI FspAI HindIII HpaI KasI KpnI MfeI MluI NaeI NarI NcoI NdeI NgoMIV NheI NotI NruI NsiI PacI PasI PciI PmeI PmlI PpiI PshAI PsiI PsrI PstI PvuI RsrII SacII SanDI SbfI SexAI SfoI SgfI SgrAI SmaI SnaBI SpeI SphI SrfI SspI StuI SwaI TaqII XmaI XmnI ZraI