Restriction map for
pFN21AB6351
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# NO CUT ENZYMES ( 153 enzymes) #
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AatII Acc65I AccI AccIII AclI
AcsI AcyI AflII AflIII AgeI
AhdI Alw44I AlwI Aor51HI ApaI
ApaLI ApoI AscI AseI AsnI
Asp700I Asp718I AspEI AspHI AspI
AvrII BalI BamHI BanII BbeI
BbrPI BbvCI BfrI BglI BlnI
BlpI BpmI Bpu1102I BsaAI BsaHI
BsaI BseAI BsgI BsiEI BsiHKAI
BsiWI BsmBI BsmI Bsp1407I BspDI
BspEI BspHI BspLU11I BsrBI BsrDI
BsrFI BsrGI BssHII BssSI Bst1107I
BstAPI BstBI BstEII BstPI BstXI
BstZ17I CelII Cfr10I ClaI CpoI
DraI DraII DraIII DrdI EagI
Eam1105I EarI EclXI Eco47III Eco52I
EcoNI EcoO109I EcoO65I EcoRI EcoRV
EcoT22I FseI Hin1I HincII HindII
HpaI KasI KpnI Ksp632I MaeII
MfeI MluI MluNI MroI MscI
MseI MslI MunI NaeI NarI
NcoI NdeI NgoMIV NheI NotI
NsiI NspV PacI PaeR7I PflFI
PflMI PinAI PleI PmaCI PmeI
PmlI PpuMI PshAI PshBI Psp1406I
PvuI PvuII RcaI RsrII SacI
SalI SapI SexAI SfaNI SfiI
SfoI SfuI SgrAI SmlI SnaBI
SpeI SspBI StuI SwaI TaqI
Tru9I Tth111I TthHB8I Van91I XbaI
XcmI XhoI XmnI
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# ONE CUT ENZYMES ( 47 enzymes) #
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AlwNI 167 CAGGCTCTG CAG[ACGT][ACGT][ACGT]^CTG
AocI 587 CCTCAGG CC^T[ACGT]AGG
AvaII 617 GGTCC G^G[AT]CC
AviII 253 TGCGCA TGC^GCA
BanI 161 GGCACC G^G[CT][GA]CC
BcgI 352 CGAAAACGCTGC (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BciVI 737 GGATAC GTATCC(6/5)
BglII 623 AGATCT A^GATCT
BmyI 160 GGGCAC G[AGT]GC[ACT]^C
BsaWI 569 TCCGGT [AT]^CCGG[AT]
Bsp1286I 160 GGGCAC G[AGT]GC[ACT]^C
BspMI 131 ACCTGC ACCTGC(4/8)
BsrI 761 CCAGT ACTGG(1/-1)
BstF5I 495 GGATG GGATG(2/0)
BstNI 166 CCAGG CC^[AT]GG
BstYI 623 AGATCT [GA]^GATC[CT]
Bsu36I 587 CCTCAGG CC^T[ACGT]AGG
Cfr13I 617 GGTCC G^G[ACGT]CC
DdeI 588 CTCAG C^T[ACGT]AG
DsaI 303 CCGCGG C^C[GA][CT]GG
EaeI 66 CGGCCA [CT]^GGCC[GA]
Eco81I 587 CCTCAGG CC^T[ACGT]AGG
EcoRII 166 CCAGG ^CC[AT]GG
EcoT14I 491 CCTTGG C^C[AT][AT]GG
FokI 495 GGATG GGATG(9/13)
FspI 253 TGCGCA TGC^GCA
HaeIII 67 GGCC GG^CC
HindIII 468 AAGCTT A^AGCTT
HinfI 194 GAATC G^A[ACGT]TC
HphI 750 TCACC GGTGA(8/7)
KspI 303 CCGCGG CCGC^GG
MflI 623 AGATCT [GA]^GATC[CT]
MvaI 166 CCAGG CC^[AT]GG
NlaIV 161 GGCACC GG[ACGT]^[ACGT]CC
NruI 41 TCGCGA TCG^CGA
NspI 362 GCATGC [GA]CATG^[CT]
SacII 303 CCGCGG CCGC^GG
Sau96I 617 GGTCC G^G[ACGT]CC
SbfI 338 CCTGCAGG CCTGCA^GG
ScaI 284 AGTACT AGT^ACT
SphI 362 GCATGC GCATG^C
Sse8387I 338 CCTGCAGG CCTGCA^GG
SspI 696 AATATT AAT^ATT
StyI 491 CCTTGG C^C[AT][AT]GG
TfiI 194 GAATC G^A[AT]TC
Tsp45I 368 GTCAC ^GT[GC]AC
XhoII 623 AGATCT [GA]^GATC[CT]
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# TWO CUT ENZYMES ( 15 enzymes) #
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AluI 24 AGCT AG^CT
469 AGCT
BbsI 417 GTCTTC GAAGAC(2/6)
522 GTCTTC
BclI 423 TGATCA T^GATCA
480 TGATCA
BpuAI 417 GTCTTC GAAGAC(2/6)
522 GTCTTC
BsaBI 424 GATCAAAATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
748 GATCACCATC
BseRI 265 GAGGAG GAGGAG(10/8)
440 GAGGAG
BsmFI 311 GGGAC GGGAC(10/14)
296 GTCCC
FbaI 423 TGATCA T^GATCA
480 TGATCA
HgaI 313 GACGC GACGC(5/10)
366 GCGTC
MaeIII 368 GTCAC ^GT[ACGT]AC
710 GTTAC
MamI 424 GATCAAAATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
748 GATCACCATC
NlaIII 363 CATG CATG^
685 CATG
PstI 173 CTGCAG CTGCA^G
339 CTGCAG
SfcI 173 CTGCAG C^T[GA][CT]AG
339 CTGCAG
TspRI 276 CACTG CA[GC]TG[ACGT][ACGT]^
462 CACTG
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# 3 OR MORE CUT ENZYMES ( 43 enzymes) #
#########################################
AccII AciI AfaI AvaI BbvI
BcnI BfaI BsaJI BsiYI BslI
BsmAI BsoBI BssKI BstUI Cac8I
CfoI DpnII FauI Fnu4HI HaeII
HapII HhaI HinP1I HpaII ItaI
MaeI MboI MboII MnlI MspA1I
MspI MvnI MwoI NciI NdeII
RsaI Sau3AI ScrFI SmaI TseI
Tsp509I XmaCI XmaI