Restriction map for
pFN21AB6351
(Linear) (Six-base) MAPSORT of: KIBB6351.seq Check: 2835 from: 1 to: 798
KIBB6351.orf
With 182 enzymes: SgfI *
April 24, 2009 13:05 ..
AlwNI CAG_nnn'CTG
Cuts at: 0 172 798
Size: 172 626
AvaI C'yCGr_G
Cuts at: 0 29 204 385 798
Size: 29 175 181 413
Fragments arranged by size:
413 181 175 29
BanI G'GyrC_C
Cuts at: 0 161 798
Size: 161 637
BbsI GAAGACnn'nnnn_
Cuts at: 0 410 515 798
Size: 410 105 283
Fragments arranged by size:
410 283 105
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 341 375 798
Size: 341 34 423
Fragments arranged by size:
423 341 34
BciVI GTATCCnnnnn_n'
Cuts at: 0 731 798
Size: 731 67
BclI T'GATC_A
Cuts at: 0 423 480 798
Size: 423 57 318
Fragments arranged by size:
423 318 57
BglII A'GATC_T
Cuts at: 0 623 798
Size: 623 175
Bme1580I G_kGCm'C
Cuts at: 0 164 798
Size: 164 634
BsaBI GATnn'nnATC
Cuts at: 0 428 752 798
Size: 428 324 46
Fragments arranged by size:
428 324 46
BsaWI w'CCGG_w
Cuts at: 0 569 798
Size: 569 229
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 280 455 798
Size: 280 175 343
Fragments arranged by size:
343 280 175
BseYI C'CCAG_C
Cuts at: 0 78 144 798
Size: 78 66 654
Fragments arranged by size:
654 78 66
Bsp1286I G_dGCh'C
Cuts at: 0 164 798
Size: 164 634
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 140 798
Size: 140 658
BstYI r'GATC_y
Cuts at: 0 623 798
Size: 623 175
Bsu36I CC'TnA_GG
Cuts at: 0 588 798
Size: 588 210
BtgI C'CryG_G
Cuts at: 0 303 798
Size: 303 495
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 0 58 798
Size: 58 740
EaeI y'GGCC_r
Cuts at: 0 66 798
Size: 66 732
FspI TGC'GCA
Cuts at: 0 255 798
Size: 255 543
HaeII r_GCGC'y
Cuts at: 0 92 155 185 351 798
Size: 92 63 30 166 447
Fragments arranged by size:
447 166 92 63 30
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 571 603 798
Size: 571 32 195
Fragments arranged by size:
571 195 32
HindIII A'AGCT_T
Cuts at: 0 468 798
Size: 468 330
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 140 798
Size: 140 658
MspA1I CmG'CkG
Cuts at: 0 19 144 305 798
Size: 19 125 161 493
Fragments arranged by size:
493 161 125 19
NruI TCG'CGA
Cuts at: 0 43 798
Size: 43 755
NspI r_CATG'y
Cuts at: 0 366 798
Size: 366 432
PstI C_TGCA'G
Cuts at: 0 177 343 798
Size: 177 166 455
Fragments arranged by size:
455 177 166
SacII CC_GC'GG
Cuts at: 0 306 798
Size: 306 492
SbfI CC_TGCA'GG
Cuts at: 0 343 798
Size: 343 455
ScaI AGT'ACT
Cuts at: 0 286 798
Size: 286 512
SfcI C'TryA_G
Cuts at: 0 173 339 798
Size: 173 166 459
Fragments arranged by size:
459 173 166
SmaI CCC'GGG
Cuts at: 0 31 206 387 798
Size: 31 175 181 411
Fragments arranged by size:
411 181 175 31
SphI G_CATG'C
Cuts at: 0 366 798
Size: 366 432
SspI AAT'ATT
Cuts at: 0 698 798
Size: 698 100
StyI C'CwwG_G
Cuts at: 0 491 798
Size: 491 307
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 179 798
Size: 179 619
TatI w'GTAC_w
Cuts at: 0 284 798
Size: 284 514
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 253 285 398 430 601 633 798
Size: 253 32 113 32 171 32 165
Fragments arranged by size:
253 171 165 113 32 32 32
XmaI C'CCGG_G
Cuts at: 0 29 204 385 798
Size: 29 175 181 413
Fragments arranged by size:
413 181 175 29
Enzymes that do cut:
AlwNI AvaI BanI BbsI BcgI BciVI BclI BglII
Bme1580I BsaBI BsaWI BseRI BseYI Bsp1286I BspMI BstYI
Bsu36I BtgI BtgZI EaeI FspI HaeII Hin4I HindIII
MmeI MspA1I NruI NspI PstI SacII SbfI ScaI
SfcI SmaI SphI SspI StyI TaqII TatI TstI
XmaI
Enzymes that do not cut:
AarI AatII AccI Acc65I AclI AcuI AfeI AflII
AflIII AgeI AhdI AleI AlfI AloI ApaI ApaLI
ApoI AscI AseI AsiSI AvrII BaeI BamHI BanII
BbeI BbvCI BfrBI BglI BlpI BmgBI BmrI BmtI
BplI BpmI Bpu10I BpuEI BsaI BsaAI BsaHI BsaXI
BsgI BsiEI BsiHKAI BsiWI BsmI BsmBI BspEI BspHI
BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI
BstEII BstXI BstZ17I BtsI ClaI CspCI DraI DraIII
DrdI EagI EarI EciI EcoICRI Eco57MI EcoNI EcoO109I
EcoRI EcoRV FalI FseI FspAI HincII HpaI KasI
KpnI MfeI MluI MscI MslI NaeI NarI NcoI
NdeI NgoMIV NheI NotI NsiI PacI PasI PciI
PflMI PfoI PmeI PmlI PpiI PpuMI PshAI PsiI
PspOMI PspXI PsrI PvuI PvuII RsrII SacI SalI
SanDI SapI SexAI SfiI SfoI SgfI SgrAI SmlI
SnaBI SpeI SrfI StuI SwaI Tth111I XbaI XcmI
XhoI XmnI ZraI