Restriction map for
pFN21AB6557
(Circular) (Six-base) MAPSORT of: pFN21AB6557.seq Check: 380 from: 1 to: 5821
pFN21AB6557 5821 bp
With 182 enzymes: SgfI *
October 20, 2009 10:41 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 2301 2301
Size: 5821
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 3631 278
Size: 53 83 186 936 2095 2468
Fragments arranged by size:
2468 2095 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 2064 3164 1937
Size: 127 1100 4594
Fragments arranged by size:
4594 1100 127
Acc65I G'GTAC_C
Cuts at: 3142 3142
Size: 5821
AclI AA'CG_TT
Cuts at: 1563 3948 4321 1563
Size: 2385 373 3063
Fragments arranged by size:
3063 2385 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 3884 5268 5536 1632
Size: 2252 1384 268 1917
Fragments arranged by size:
2252 1917 1384 268
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 5624
AflIII A'CryG_T
Cuts at: 1203 4720 5697 1203
Size: 3517 977 1327
Fragments arranged by size:
3517 1327 977
AgeI A'CCGG_T
Cuts at: 4652 4652
Size: 5821
AhdI GACnn_n'nnGTC
Cuts at: 2710 4550 2710
Size: 1840 3981
AleI CACnn'nnGTG
Cuts at: 1166 2606 1166
Size: 1440 4381
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 5821
AlwNI CAG_nnn'CTG
Cuts at: 3564 5136 3564
Size: 1572 4249
ApaLI G'TGCA_C
Cuts at: 3878 5034 3878
Size: 1156 4665
ApoI r'AATT_y
Cuts at: 1493 3130 3356 3508 5617 1493
Size: 1637 226 152 2109 1697
Fragments arranged by size:
2109 1697 1637 226 152
AseI AT'TA_AT
Cuts at: 160 1032 4375 160
Size: 872 3343 1606
Fragments arranged by size:
3343 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5821
AvaI C'yCGr_G
Cuts at: 1870 1943 3146 1870
Size: 73 1203 4545
Fragments arranged by size:
4545 1203 73
BamHI G'GATC_C
Cuts at: 3151 3518 3151
Size: 367 5454
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2092 2289 2983 3046 3142
Size: 325 217 627 305 197 694 63 96
Cuts at: 3142 4597 618
Size: 1455 1842
Fragments arranged by size:
1842 1455 694 627 325 305 217 197
96 63
BanII G_rGCy'C
Cuts at: 1391 2123 3140 1391
Size: 732 1017 4072
Fragments arranged by size:
4072 1017 732
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 928
Size: 971 4850
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 4012 4046 1903
Size: 34 2075 34 3678
Fragments arranged by size:
3678 2075 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 3713 4923 3713
Size: 1210 4611
BclI T'GATC_A
Cuts at: 1548 1548
Size: 5821
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 2256 4432 136
Size: 107 122 71 631 605 584 2176 1525
Fragments arranged by size:
2176 1525 631 605 584 122 107 71
BglII A'GATC_T
Cuts at: 3003 5816 3003
Size: 2813 3008
BlpI GC'TnA_GC
Cuts at: 2345 3238 2345
Size: 893 4928
Bme1580I G_kGCm'C
Cuts at: 1790 2652 3882 5038 1790
Size: 862 1230 1156 2573
Fragments arranged by size:
2573 1230 1156 862
BmgBI CAC'GTC
Cuts at: 1318 2128 2172 2679 1318
Size: 810 44 507 4460
Fragments arranged by size:
4460 810 507 44
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 2164 2255 3552
Size: 416 53 83 399 24 747 91 1297
Cuts at: 3552 4510 442
Size: 958 1753
Fragments arranged by size:
1753 1297 958 747 416 399 91 83
53 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 5821
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2607 2690 2902 4481 1239
Size: 1368 83 212 1579 2579
Fragments arranged by size:
2579 1579 1368 212 83
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 2468 2708 3946 4811 5109 2468
Size: 240 1238 865 298 3180
Fragments arranged by size:
3180 1238 865 298 240
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 2545 2948 4484 882
Size: 617 1046 403 1536 2219
Fragments arranged by size:
2219 1536 1046 617 403
BsaAI yAC'GTr
Cuts at: 493 1204 3612 493
Size: 711 2408 2702
Fragments arranged by size:
2702 2408 711
BsaBI GATnn'nnATC
Cuts at: 5815 5815
Size: 5821
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 3628 4010 4645 275
Size: 53 83 186 936 2095 382 635 1451
Fragments arranged by size:
2095 1451 936 635 382 186 83 53
BsaWI w'CCGG_w
Cuts at: 4254 4652 4926 5073 5484 4254
Size: 398 274 147 411 4591
Fragments arranged by size:
4591 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 2267 2297 2504 2525 2534
Size: 30 165 30 864 30 207 21 9
Cuts at: 2534 2555 2663 2693 1178
Size: 21 108 30 4306
Fragments arranged by size:
4306 864 207 165 108 30 30 30
30 21 21 9
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 1186
Size: 5821
BseYI C'CCAG_C
Cuts at: 2476 2651 3022 5024 5520 2476
Size: 175 371 2002 496 2777
Fragments arranged by size:
2777 2002 496 371 175
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2123 1163
Size: 573 387 4861
Fragments arranged by size:
4861 573 387
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 3271 4032 5060 1540
Size: 275 186 1270 761 1028 2301
Fragments arranged by size:
2301 1270 1028 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 2232 2346 3882 3967 5038 2232
Size: 114 1536 85 1071 3015
Fragments arranged by size:
3015 1536 1071 114 85
BsmI GAATG_Cn'
Cuts at: 3332 3425 3332
Size: 93 5728
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2123 2232 2346 2652 3140 3882 3967
Size: 399 333 109 114 306 488 742 85
Cuts at: 3967 5038 1391
Size: 1071 2174
Fragments arranged by size:
2174 1071 742 488 399 333 306 114
109 85
BspEI T'CCGG_A
Cuts at: 5484 5484
Size: 5821
BspHI T'CATG_A
Cuts at: 3710 5440 3710
Size: 1730 4091
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2214 2301 3176 844
Size: 1370 87 875 3489
Fragments arranged by size:
3489 1370 875 87
BsrBI CCG'CTC
Cuts at: 1394 1823 2355 3708 1394
Size: 429 532 1353 3507
Fragments arranged by size:
3507 1353 532 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 2563 4316 4490 65
Size: 1183 1315 1753 174 1396
Fragments arranged by size:
1753 1396 1315 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 2664 4465 4652 1700
Size: 110 854 1801 187 2869
Fragments arranged by size:
2869 1801 854 187 110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 5821
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 5821
BssSI C'ACGA_G
Cuts at: 3881 4893 3881
Size: 1012 4809
BstAPI GCAn_nnn'nTGC
Cuts at: 794 794
Size: 5821
BstBI TT'CG_AA
Cuts at: 4625 4625
Size: 5821
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 5821
BstXI CCAn_nnnn'nTGG
Cuts at: 2458 2980 3076 2458
Size: 522 96 5203
Fragments arranged by size:
5203 522 96
BstYI r'GATC_y
Cuts at: 1972 3003 3151 3518 3903 3920 5372 5458 5470
Size: 1031 148 367 385 17 1452 86 12
Cuts at: 5470 5816 1972
Size: 346 1977
Fragments arranged by size:
1977 1452 1031 385 367 346 148 86
17 12
BtgI C'CryG_G
Cuts at: 513 1065 2004 2021 2233 2330 2934 513
Size: 552 939 17 212 97 604 3400
Fragments arranged by size:
3400 939 604 552 212 97 17
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 5082
Fragments arranged by size:
5082 683 56
BtsI GCAGTG_nn'
Cuts at: 2132 3099 3337 4131 4151 2132
Size: 967 238 794 20 3802
Fragments arranged by size:
3802 967 794 238 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 5821
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 5786
DraI TTT'AAA
Cuts at: 3125 3125
Size: 5821
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 4828 809
Size: 507 3512 1802
Fragments arranged by size:
3512 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2642 3268 4157
Size: 54 1425 50 275 18 812 626 889
Cuts at: 4157 5786 8
Size: 1629 43
Fragments arranged by size:
1629 1425 889 812 626 275 54 50
43 18
EagI C'GGCC_G
Cuts at: 1537 1812 3268 1537
Size: 275 1456 4090
Fragments arranged by size:
4090 1456 275
EarI CTCTTCn'nnn_
Cuts at: 1353 3751 1353
Size: 2398 3423
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 4408 4782 4928 387
Size: 1064 138 2819 374 146 1280
Fragments arranged by size:
2819 1280 1064 374 146 138
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2607 2690 2902 3884 4481 5268 5536
Size: 393 975 83 212 982 597 787 268
Cuts at: 5536 1239
Size: 1524
Fragments arranged by size:
1524 982 975 787 597 393 268 212
83
EcoO109I rG'GnC_Cy
Cuts at: 2473 3038 2473
Size: 565 5256
EcoRI G'AATT_C
Cuts at: 3130 3508 3130
Size: 378 5443
FspI TGC'GCA
Cuts at: 4327 4327
Size: 5821
HaeII r_GCGC'y
Cuts at: 1678 2116 4968 1678
Size: 438 2852 2531
Fragments arranged by size:
2852 2531 438
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2065 2097 2696 2728 2736
Size: 32 135 32 493 32 599 32 8
Cuts at: 2736 2768 4462 4494 4536 4568 1373
Size: 32 1694 32 42 32 2626
Fragments arranged by size:
2626 1694 599 493 135 42 32 32
32 32 32 32 32 8
HincII GTy'rAC
Cuts at: 669 1657 1938 3165 3411 669
Size: 988 281 1227 246 3079
Fragments arranged by size:
3079 1227 988 281 246
HindIII A'AGCT_T
Cuts at: 748 748
Size: 5821
HpaI GTT'AAC
Cuts at: 3411 3411
Size: 5821
KpnI G_GTAC'C
Cuts at: 3146 3146
Size: 5821
MfeI C'AATT_G
Cuts at: 3420 3420
Size: 5821
MluI A'CGCG_T
Cuts at: 5697 5697
Size: 5821
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 2382 2808 4935 5119 1305
Size: 1077 426 2127 184 2007
Fragments arranged by size:
2127 2007 1077 426 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 10
Size: 54 1425 343 3999
Fragments arranged by size:
3999 1425 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2137 2606 3779 4138 4297 518
Size: 648 971 469 1173 359 159 2042
Fragments arranged by size:
2042 1173 971 648 469 359 159
MspA1I CmG'CkG
Cuts at: 1606 2224 2332 2349 2388 2493 3030 3092 3237
Size: 618 108 17 39 105 537 62 145
Cuts at: 3237 3914 5062 5307 1606
Size: 677 1148 245 2120
Fragments arranged by size:
2120 1148 677 618 537 245 145 108
105 62 39 17
NaeI GCC'GGC
Cuts at: 2666 2666
Size: 5821
NcoI C'CATG_G
Cuts at: 513 1065 2004 2233 513
Size: 552 939 229 4101
Fragments arranged by size:
4101 939 552 229
NdeI CA'TA_TG
Cuts at: 387 387
Size: 5821
NgoMIV G'CCGG_C
Cuts at: 2664 2664
Size: 5821
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 5821
NotI GC'GGCC_GC
Cuts at: 3268 3268
Size: 5821
NspI r_CATG'y
Cuts at: 1132 1229 2318 2512 2774 3179 4724 1132
Size: 97 1089 194 262 405 1545 2229
Fragments arranged by size:
2229 1545 1089 405 262 194 97
PasI CC'CwG_GG
Cuts at: 1348 2563 3014 1348
Size: 1215 451 4155
Fragments arranged by size:
4155 1215 451
PciI A'CATG_T
Cuts at: 4720 4720
Size: 5821
PflMI CCAn_nnn'nTGG
Cuts at: 1108 2239 1108
Size: 1131 4690
PfoI T'CCnGG_A
Cuts at: 2754 2754
Size: 5821
PmeI GTTT'AAAC
Cuts at: 3125 3125
Size: 5821
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3890 3922 5403 5435 3890
Size: 32 1481 32 4276
Fragments arranged by size:
4276 1481 32 32
PpuMI rG'GwC_Cy
Cuts at: 2473 3038 2473
Size: 565 5256
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 5821
PsiI TTA'TAA
Cuts at: 3391 3391
Size: 5821
PstI C_TGCA'G
Cuts at: 830 2387 3173 830
Size: 1557 786 3478
Fragments arranged by size:
3478 1557 786
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 5821
PvuII CAG'CTG
Cuts at: 2224 2349 2388 2224
Size: 125 39 5657
Fragments arranged by size:
5657 125 39
SacII CC_GC'GG
Cuts at: 2333 2333
Size: 5821
SalI G'TCGA_C
Cuts at: 1936 3163 1936
Size: 1227 4594
SanDI GG'GwC_CC
Cuts at: 3038 3038
Size: 5821
SbfI CC_TGCA'GG
Cuts at: 3173 3173
Size: 5821
ScaI AGT'ACT
Cuts at: 4069 5781 4069
Size: 1712 4109
SfcI C'TryA_G
Cuts at: 826 1045 2383 3169 4304 4985 5176 826
Size: 219 1338 786 1135 681 191 1471
Fragments arranged by size:
1471 1338 1135 786 681 219 191
SfiI GGCCn_nnn'nGGCC
Cuts at: 2256 2256
Size: 5821
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5821
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 5821
SmaI CCC'GGG
Cuts at: 1872 3148 1872
Size: 1276 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 2483 2723 3925 4826 5088 820
Size: 197 926 540 240 1202 901 262 1553
Fragments arranged by size:
1553 1202 926 901 540 262 240 197
SnaBI TAC'GTA
Cuts at: 493 3612 493
Size: 3119 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 5821
SphI G_CATG'C
Cuts at: 1132 3179 1132
Size: 2047 3774
SspI AAT'ATT
Cuts at: 5 52 3745 5
Size: 47 3693 2081
Fragments arranged by size:
3693 2081 47
StuI AGG'CCT
Cuts at: 1517 1517
Size: 5821
StyI C'CwwG_G
Cuts at: 513 1065 2004 2233 2370 2850 3260 513
Size: 552 939 229 137 480 410 3074
Fragments arranged by size:
3074 939 552 480 410 229 137
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 3848 3865 4018 4203 4648 325
Size: 1255 2268 17 153 185 445 1498
Fragments arranged by size:
2268 1498 1255 445 185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 2532 2829 4067
Size: 274 80 33 51 1443 555 297 1238
Cuts at: 4067 5779 96
Size: 1712 138
Fragments arranged by size:
1712 1443 1238 555 297 274 138 80
51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2267 2299 2398 2430 2504 2536 2663 2695 2860
Size: 32 99 32 74 32 127 32 165
Cuts at: 2860 2892 5781 5813 2267
Size: 32 2889 32 2275
Fragments arranged by size:
2889 2275 165 127 99 74 32 32
32 32 32 32
Tth111I GACn'n_nGTC
Cuts at: 1316 2079 1316
Size: 763 5058
XbaI T'CTAG_A
Cuts at: 3157 3157
Size: 5821
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 2977 2977
Size: 5821
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 5821
XmaI C'CCGG_G
Cuts at: 1870 3146 1870
Size: 1276 4545
XmnI GAAnn'nnTTC
Cuts at: 3950 3950
Size: 5821
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 3629 276
Size: 53 83 186 936 2095 2468
Fragments arranged by size:
2468 2095 936 186 83 53
Enzymes that do cut:
AarI AatII AccI Acc65I AclI AcuI AflII AflIII
AgeI AhdI AleI AlfI AlwNI ApaLI ApoI AseI
AsiSI AvaI BamHI BanI BanII BbsI BcgI BciVI
BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI
BpmI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI
BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI
BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI
BstAPI BstBI BstEII BstXI BstYI BtgI BtgZI BtsI
ClaI CspCI DraI DrdI EaeI EagI EarI EciI
Eco57MI EcoO109I EcoRI FspI HaeII Hin4I HincII HindIII
HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I
NaeI NcoI NdeI NgoMIV NheI NotI NspI PasI
PciI PflMI PfoI PmeI PpiI PpuMI PshAI PsiI
PstI PvuI PvuII SacII SalI SanDI SbfI ScaI
SfcI SfiI SgfI SgrAI SmaI SmlI SnaBI SpeI
SphI SspI StuI StyI TaqII TatI TstI Tth111I
XbaI XcmI XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AfeI AloI ApaI AscI AvrII BaeI BbeI BbvCI
BfrBI BplI Bpu10I BsiWI BsmBI BstZ17I Bsu36I DraIII
EcoICRI EcoNI EcoRV FalI FseI FspAI KasI NarI
NruI NsiI PacI PmlI PspOMI PspXI PsrI RsrII
SacI SapI SexAI SfoI SrfI SwaI