(Circular) (Six-base) MAPSORT of: pFN21AE1566.seq Check: 3967 from: 1 to: 6130 pFN21AE1566 6130 bp With 182 enzymes: SgfI * August 16, 2010 14:54 .. AatII G_ACGT'C Cuts at: 278 331 414 600 1536 3940 278 Size: 53 83 186 936 2404 2468 Fragments arranged by size: 2468 2404 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 3473 1937 Size: 1536 4594 Acc65I G'GTAC_C Cuts at: 2611 3451 2611 Size: 840 5290 AclI AA'CG_TT Cuts at: 1563 4257 4630 1563 Size: 2694 373 3063 Fragments arranged by size: 3063 2694 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 2936 3234 4193 5577 5845 1632 Size: 1304 298 959 1384 268 1917 Fragments arranged by size: 1917 1384 1304 959 298 268 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 5933 AflIII A'CryG_T Cuts at: 1203 5029 6006 1203 Size: 3826 977 1327 Fragments arranged by size: 3826 1327 977 AgeI A'CCGG_T Cuts at: 4961 4961 Size: 6130 AhdI GACnn_n'nnGTC Cuts at: 4859 4859 Size: 6130 AleI CACnn'nnGTG Cuts at: 1166 1166 Size: 6130 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 1184 Size: 6130 AlwNI CAG_nnn'CTG Cuts at: 2023 2230 2684 3873 5445 2023 Size: 207 454 1189 1572 2708 Fragments arranged by size: 2708 1572 1189 454 207 ApaI G_GGCC'C Cuts at: 2059 2059 Size: 6130 ApaLI G'TGCA_C Cuts at: 4187 5343 4187 Size: 1156 4974 ApoI r'AATT_y Cuts at: 1493 3439 3665 3817 5926 1493 Size: 1946 226 152 2109 1697 Fragments arranged by size: 2109 1946 1697 226 152 AseI AT'TA_AT Cuts at: 160 1032 4684 160 Size: 872 3652 1606 Fragments arranged by size: 3652 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 6130 AvaI C'yCGr_G Cuts at: 1870 1943 2041 3455 1870 Size: 73 98 1414 4545 Fragments arranged by size: 4545 1414 98 73 BamHI G'GATC_C Cuts at: 3460 3827 3460 Size: 367 5763 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2611 3451 4906 618 Size: 325 217 627 824 840 1455 1842 Fragments arranged by size: 1842 1455 840 824 627 325 217 BanII G_rGCy'C Cuts at: 1391 2059 2143 2669 2906 3235 3449 1391 Size: 668 84 526 237 329 214 4072 Fragments arranged by size: 4072 668 526 329 237 214 84 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 928 Size: 971 5159 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 4321 4355 1903 Size: 34 2384 34 3678 Fragments arranged by size: 3678 2384 34 34 BciVI GTATCCnnnnn_n' Cuts at: 4022 5232 4022 Size: 1210 4920 BclI T'GATC_A Cuts at: 1548 1548 Size: 6130 BfrBI ATG'CAT Cuts at: 3418 3418 Size: 6130 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 4741 136 Size: 107 122 71 631 605 3069 1525 Fragments arranged by size: 3069 1525 631 605 122 107 71 BglII A'GATC_T Cuts at: 6125 6125 Size: 6130 BlpI GC'TnA_GC Cuts at: 3547 3547 Size: 6130 Bme1580I G_kGCm'C Cuts at: 1790 2059 4191 5347 1790 Size: 269 2132 1156 2573 Fragments arranged by size: 2573 2132 1156 269 BmgBI CAC'GTC Cuts at: 1318 1318 Size: 6130 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 2817 3390 3861 Size: 416 53 83 399 24 1400 573 471 Cuts at: 3861 4819 442 Size: 958 1753 Fragments arranged by size: 1753 1400 958 573 471 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 6130 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2367 2589 2609 3141 4790 1239 Size: 1128 222 20 532 1649 2579 Fragments arranged by size: 2579 1649 1128 532 222 20 Bpu10I CC'TnA_GC Cuts at: 2176 2176 Size: 6130 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 4255 5120 5418 4255 Size: 865 298 4967 Fragments arranged by size: 4967 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 4793 882 Size: 617 3294 2219 Fragments arranged by size: 3294 2219 617 BsaAI yAC'GTr Cuts at: 493 1204 2626 3921 493 Size: 711 1422 1295 2702 Fragments arranged by size: 2702 1422 1295 711 BsaBI GATnn'nnATC Cuts at: 6124 6124 Size: 6130 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 2920 3937 4319 4954 Size: 53 83 186 936 1387 1017 382 635 Cuts at: 4954 275 Size: 1451 Fragments arranged by size: 1451 1387 1017 936 635 382 186 83 53 BsaWI w'CCGG_w Cuts at: 4563 4961 5235 5382 5793 4563 Size: 398 274 147 411 4900 Fragments arranged by size: 4900 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 2451 2481 2497 2527 2743 Size: 30 165 30 1048 30 16 30 216 Cuts at: 2743 2773 2800 2830 3082 3112 1178 Size: 30 27 30 252 30 4196 Fragments arranged by size: 4196 1048 252 216 165 30 30 30 30 30 30 30 27 16 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2808 2997 3000 3003 3027 3138 3141 3144 Size: 1622 189 3 3 24 111 3 3 Cuts at: 3144 3188 1186 Size: 44 4128 Fragments arranged by size: 4128 1622 189 111 44 24 3 3 3 3 BseYI C'CCAG_C Cuts at: 2247 5333 5829 2247 Size: 3086 496 2548 Fragments arranged by size: 3086 2548 496 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 1163 Size: 573 5557 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 3580 4341 5369 1540 Size: 275 186 1579 761 1028 2301 Fragments arranged by size: 2301 1579 1028 761 275 186 BsiHKAI G_wGCw'C Cuts at: 4191 4276 5347 4191 Size: 85 1071 4974 Fragments arranged by size: 4974 1071 85 BsmI GAATG_Cn' Cuts at: 3641 3734 3641 Size: 93 6037 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2059 2143 2669 2906 3235 3449 4191 Size: 399 269 84 526 237 329 214 742 Cuts at: 4191 4276 5347 1391 Size: 85 1071 2174 Fragments arranged by size: 2174 1071 742 526 399 329 269 237 214 85 84 BspEI T'CCGG_A Cuts at: 5793 5793 Size: 6130 BspHI T'CATG_A Cuts at: 4019 5749 4019 Size: 1730 4400 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 3485 844 Size: 2641 3489 BsrBI CCG'CTC Cuts at: 1394 1823 4017 1394 Size: 429 2194 3507 Fragments arranged by size: 3507 2194 429 BsrDI GCAATG_nn' Cuts at: 65 1248 4625 4799 65 Size: 1183 3377 174 1396 Fragments arranged by size: 3377 1396 1183 174 BsrFI r'CCGG_y Cuts at: 1700 1810 4774 4961 1700 Size: 110 2964 187 2869 Fragments arranged by size: 2964 2869 187 110 BsrGI T'GTAC_A Cuts at: 96 2845 96 Size: 2749 3381 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 6130 BssSI C'ACGA_G Cuts at: 4190 5202 4190 Size: 1012 5118 BstAPI GCAn_nnn'nTGC Cuts at: 794 2023 2186 794 Size: 1229 163 4738 Fragments arranged by size: 4738 1229 163 BstBI TT'CG_AA Cuts at: 4934 4934 Size: 6130 BstEII G'GTnAC_C Cuts at: 1184 1184 Size: 6130 BstYI r'GATC_y Cuts at: 1972 3460 3827 4212 4229 5681 5767 5779 6125 Size: 1488 367 385 17 1452 86 12 346 Cuts at: 6125 1972 Size: 1977 Fragments arranged by size: 1977 1488 1452 385 367 346 86 17 12 BtgI C'CryG_G Cuts at: 513 1065 2004 3227 513 Size: 552 939 1223 3416 Fragments arranged by size: 3416 1223 939 552 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 487 Size: 683 56 5391 Fragments arranged by size: 5391 683 56 BtsI GCAGTG_nn' Cuts at: 2722 3646 4440 4460 2722 Size: 924 794 20 4392 Fragments arranged by size: 4392 924 794 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 6130 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 6095 DraI TTT'AAA Cuts at: 3434 3434 Size: 6130 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 5137 809 Size: 507 3821 1802 Fragments arranged by size: 3821 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2091 2208 3063 Size: 54 1425 50 275 18 261 117 855 Cuts at: 3063 3577 4466 6095 8 Size: 514 889 1629 43 Fragments arranged by size: 1629 1425 889 855 514 275 261 117 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 3577 1537 Size: 275 1765 4090 Fragments arranged by size: 4090 1765 275 EarI CTCTTCn'nnn_ Cuts at: 1353 2025 2066 2317 3165 3210 4060 1353 Size: 672 41 251 848 45 850 3423 Fragments arranged by size: 3423 850 848 672 251 45 41 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 2024 4717 5091 5237 387 Size: 1064 138 435 2693 374 146 1280 Fragments arranged by size: 2693 1280 1064 435 374 146 138 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2367 2589 2609 2936 3141 3234 4193 Size: 393 735 222 20 327 205 93 959 Cuts at: 4193 4790 5577 5845 1239 Size: 597 787 268 1524 Fragments arranged by size: 1524 959 787 735 597 393 327 268 222 205 93 20 EcoNI CCTnn'n_nnAGG Cuts at: 2544 2544 Size: 6130 EcoO109I rG'GnC_Cy Cuts at: 2056 2972 3220 2056 Size: 916 248 4966 Fragments arranged by size: 4966 916 248 EcoRI G'AATT_C Cuts at: 3439 3817 3439 Size: 378 5752 EcoRV GAT'ATC Cuts at: 2236 2236 Size: 6130 FalI AAGnnnnnCTTnnnnnnnn_nnnnn' Cuts at: 2576 2576 Size: 6130 FspI TGC'GCA Cuts at: 4636 4636 Size: 6130 HaeII r_GCGC'y Cuts at: 1678 5277 1678 Size: 3599 2531 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2365 2397 2743 2775 3284 Size: 32 135 32 793 32 346 32 509 Cuts at: 3284 3316 4771 4803 4845 4877 1373 Size: 32 1455 32 42 32 2626 Fragments arranged by size: 2626 1455 793 509 346 135 42 32 32 32 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 3474 3720 669 Size: 988 281 1536 246 3079 Fragments arranged by size: 3079 1536 988 281 246 HindIII A'AGCT_T Cuts at: 748 748 Size: 6130 HpaI GTT'AAC Cuts at: 3720 3720 Size: 6130 KpnI G_GTAC'C Cuts at: 2615 3455 2615 Size: 840 5290 MfeI C'AATT_G Cuts at: 3729 3729 Size: 6130 MluI A'CGCG_T Cuts at: 6006 6006 Size: 6130 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 2540 2729 5244 5428 1305 Size: 1235 189 2515 184 2007 Fragments arranged by size: 2515 2007 1235 189 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 2210 3065 10 Size: 54 1425 343 378 855 3075 Fragments arranged by size: 3075 1425 855 378 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 2486 4088 4447 4606 518 Size: 648 1320 1602 359 159 2042 Fragments arranged by size: 2042 1602 1320 648 359 159 MspA1I CmG'CkG Cuts at: 1606 3546 4223 5371 5616 1606 Size: 1940 677 1148 245 2120 Fragments arranged by size: 2120 1940 1148 677 245 NcoI C'CATG_G Cuts at: 513 1065 2004 513 Size: 552 939 4639 Fragments arranged by size: 4639 939 552 NdeI CA'TA_TG Cuts at: 387 387 Size: 6130 NheI G'CTAG_C Cuts at: 1052 1052 Size: 6130 NotI GC'GGCC_GC Cuts at: 3577 3577 Size: 6130 NsiI A_TGCA'T Cuts at: 3420 3420 Size: 6130 NspI r_CATG'y Cuts at: 1132 1229 3488 5033 1132 Size: 97 2259 1545 2229 Fragments arranged by size: 2259 2229 1545 97 PasI CC'CwG_GG Cuts at: 1348 1348 Size: 6130 PciI A'CATG_T Cuts at: 5029 5029 Size: 6130 PflMI CCAn_nnn'nTGG Cuts at: 1108 1108 Size: 6130 PmeI GTTT'AAAC Cuts at: 3434 3434 Size: 6130 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 4199 4231 5712 5744 4199 Size: 32 1481 32 4585 Fragments arranged by size: 4585 1481 32 32 PpuMI rG'GwC_Cy Cuts at: 2972 3220 2972 Size: 248 5882 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 6130 PsiI TTA'TAA Cuts at: 3700 3700 Size: 6130 PspOMI G'GGCC_C Cuts at: 2055 2055 Size: 6130 PstI C_TGCA'G Cuts at: 830 2019 2226 3482 830 Size: 1189 207 1256 3478 Fragments arranged by size: 3478 1256 1189 207 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 6130 SalI G'TCGA_C Cuts at: 1936 3472 1936 Size: 1536 4594 SbfI CC_TGCA'GG Cuts at: 3482 3482 Size: 6130 ScaI AGT'ACT Cuts at: 4378 6090 4378 Size: 1712 4418 SfcI C'TryA_G Cuts at: 826 1045 2015 2222 2854 2914 3026 3478 4613 Size: 219 970 207 632 60 112 452 1135 Cuts at: 4613 5294 5485 826 Size: 681 191 1471 Fragments arranged by size: 1471 1135 970 681 632 452 219 207 191 112 60 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 6130 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 6130 SmaI CCC'GGG Cuts at: 1872 2043 3457 1872 Size: 171 1414 4545 Fragments arranged by size: 4545 1414 171 SmlI C'TyrA_G Cuts at: 820 1017 1943 4234 5135 5397 820 Size: 197 926 2291 901 262 1553 Fragments arranged by size: 2291 1553 926 901 262 197 SnaBI TAC'GTA Cuts at: 493 2626 3921 493 Size: 2133 1295 2702 Fragments arranged by size: 2702 2133 1295 SpeI A'CTAG_T Cuts at: 152 152 Size: 6130 SphI G_CATG'C Cuts at: 1132 3488 1132 Size: 2356 3774 SspI AAT'ATT Cuts at: 5 52 3082 4054 5 Size: 47 3030 972 2081 Fragments arranged by size: 3030 2081 972 47 StuI AGG'CCT Cuts at: 1517 2869 1517 Size: 1352 4778 StyI C'CwwG_G Cuts at: 513 1065 2004 2205 3569 513 Size: 552 939 201 1364 3074 Fragments arranged by size: 3074 1364 939 552 201 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 2183 2480 2754 4157 4174 4327 4512 Size: 1255 603 297 274 1403 17 153 185 Cuts at: 4512 4957 325 Size: 445 1498 Fragments arranged by size: 1498 1403 1255 603 445 297 274 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 2845 4376 6088 Size: 274 80 33 51 1443 868 1531 1712 Cuts at: 6088 96 Size: 138 Fragments arranged by size: 1712 1531 1443 868 274 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 2451 2483 3212 3244 6090 6122 2451 Size: 32 729 32 2846 32 2459 Fragments arranged by size: 2846 2459 729 32 32 32 Tth111I GACn'n_nGTC Cuts at: 1316 1316 Size: 6130 XbaI T'CTAG_A Cuts at: 2440 3466 2440 Size: 1026 5104 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 6130 XmaI C'CCGG_G Cuts at: 1870 2041 3455 1870 Size: 171 1414 4545 Fragments arranged by size: 4545 1414 171 XmnI GAAnn'nnTTC Cuts at: 4259 4259 Size: 6130 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 3938 276 Size: 53 83 186 936 2404 2468 Fragments arranged by size: 2468 2404 936 186 83 53 Enzymes that do cut: AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbsI BcgI BciVI BclI BfrBI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstYI BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI EarI EciI Eco57MI EcoNI EcoO109I EcoRI EcoRV FalI FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I NcoI NdeI NheI NotI NsiI NspI PasI PciI PflMI PmeI PpiI PpuMI PshAI PsiI PspOMI PstI PvuI SalI SbfI ScaI SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XhoI XmaI XmnI ZraI Enzymes that do not cut: AarI AfeI AloI AscI AvrII BaeI BbeI BbvCI BplI BsiWI BsmBI BstXI BstZ17I Bsu36I DraIII EcoICRI FseI FspAI KasI NaeI NarI NgoMIV NruI PacI PfoI PmlI PspXI PsrI PvuII RsrII SacI SacII SanDI SapI SexAI SfiI SfoI SrfI SwaI XcmI