Restriction map for
pFN21AB9951
(Circular) (Six-base) MAPSORT of: pFN21AB9951.seq Check: 6670 from: 1 to: 6310
pFN21AB9951 6310 bp
With 182 enzymes: SgfI *
April 15, 2010 09:28 ..
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 4120 278
Size: 53 83 186 936 2584 2468
Fragments arranged by size:
2584 2468 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 3653 1937
Size: 1716 4594
Acc65I G'GTAC_C
Cuts at: 3631 3631
Size: 6310
AclI AA'CG_TT
Cuts at: 1563 4437 4810 1563
Size: 2874 373 3063
Fragments arranged by size:
3063 2874 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2055 2498 2510 3422 4373 5757 6025 1632
Size: 423 443 12 912 951 1384 268 1917
Fragments arranged by size:
1917 1384 951 912 443 423 268 12
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 6113
AflIII A'CryG_T
Cuts at: 1203 2460 5209 6186 1203
Size: 1257 2749 977 1327
Fragments arranged by size:
2749 1327 1257 977
AgeI A'CCGG_T
Cuts at: 5141 5141
Size: 6310
AhdI GACnn_n'nnGTC
Cuts at: 5039 5039
Size: 6310
AleI CACnn'nnGTG
Cuts at: 1166 2583 2759 1166
Size: 1417 176 4717
Fragments arranged by size:
4717 1417 176
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 3353 1184
Size: 2169 4141
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2955 2987 2955
Size: 32 6278
AlwNI CAG_nnn'CTG
Cuts at: 2078 3593 4053 5625 2078
Size: 1515 460 1572 2763
Fragments arranged by size:
2763 1572 1515 460
ApaLI G'TGCA_C
Cuts at: 2545 2799 4367 5523 2545
Size: 254 1568 1156 3332
Fragments arranged by size:
3332 1568 1156 254
ApoI r'AATT_y
Cuts at: 1493 3179 3619 3845 3997 6106 1493
Size: 1686 440 226 152 2109 1697
Fragments arranged by size:
2109 1697 1686 440 226 152
AseI AT'TA_AT
Cuts at: 160 1032 4864 160
Size: 872 3832 1606
Fragments arranged by size:
3832 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6310
AvaI C'yCGr_G
Cuts at: 1870 1943 3464 3635 1870
Size: 73 1521 171 4545
Fragments arranged by size:
4545 1521 171 73
BamHI G'GATC_C
Cuts at: 3640 4007 3640
Size: 367 5943
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 3426 3631 5086 618
Size: 325 217 627 1639 205 1455 1842
Fragments arranged by size:
1842 1639 1455 627 325 217 205
BanII G_rGCy'C
Cuts at: 1391 3629 1391
Size: 2238 4072
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 2501 2702 2898 928
Size: 971 602 201 196 4340
Fragments arranged by size:
4340 971 602 201 196
BbvCI CC'TCA_GC
Cuts at: 2119 3315 2119
Size: 1196 5114
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 4501 4535 1903
Size: 34 2564 34 3678
Fragments arranged by size:
3678 2564 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 2257 3079 4202 5412 2257
Size: 822 1123 1210 3155
Fragments arranged by size:
3155 1210 1123 822
BclI T'GATC_A
Cuts at: 1548 1548
Size: 6310
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 2188 3101 4921
Size: 107 122 71 631 605 516 913 1820
Cuts at: 4921 136
Size: 1525
Fragments arranged by size:
1820 1525 913 631 605 516 122 107
71
BglII A'GATC_T
Cuts at: 6305 6305
Size: 6310
BlpI GC'TnA_GC
Cuts at: 3477 3727 3477
Size: 250 6060
Bme1580I G_kGCm'C
Cuts at: 1790 2549 2803 2997 3282 4371 5527 1790
Size: 759 254 194 285 1089 1156 2573
Fragments arranged by size:
2573 1156 1089 759 285 254 194
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 6310
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 4041 4999 442
Size: 416 53 83 399 24 2624 958 1753
Fragments arranged by size:
2624 1753 958 416 399 83 53 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 6310
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2825 2840 4970 1239
Size: 1586 15 2130 2579
Fragments arranged by size:
2579 2130 1586 15
Bpu10I CC'TnA_GC
Cuts at: 2119 2567 3315 3431 2119
Size: 448 748 116 4998
Fragments arranged by size:
4998 748 448 116
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 4435 5300 5598 4435
Size: 865 298 5147
Fragments arranged by size:
5147 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 3536 4973 882
Size: 617 2037 1437 2219
Fragments arranged by size:
2219 2037 1437 617
BsaAI yAC'GTr
Cuts at: 493 1204 4101 493
Size: 711 2897 2702
Fragments arranged by size:
2897 2702 711
BsaBI GATnn'nnATC
Cuts at: 6304 6304
Size: 6310
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 4117 4499 5134 275
Size: 53 83 186 936 2584 382 635 1451
Fragments arranged by size:
2584 1451 936 635 382 186 83 53
BsaWI w'CCGG_w
Cuts at: 4743 5141 5415 5562 5973 4743
Size: 398 274 147 411 5080
Fragments arranged by size:
5080 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 2003 2033 2564 2594 2768
Size: 30 165 30 600 30 531 30 174
Cuts at: 2768 2798 1178
Size: 30 4690
Fragments arranged by size:
4690 600 531 174 165 30 30 30
30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 3080 3272 3383 3595 1186
Size: 1894 192 111 212 3901
Fragments arranged by size:
3901 1894 212 192 111
BseYI C'CCAG_C
Cuts at: 2977 3240 3490 5513 6009 2977
Size: 263 250 2023 496 3278
Fragments arranged by size:
3278 2023 496 263 250
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2561 3506 1163
Size: 573 825 945 3967
Fragments arranged by size:
3967 945 825 573
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 3760 4521 5549 1540
Size: 275 186 1759 761 1028 2301
Fragments arranged by size:
2301 1759 1028 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 2549 2803 4371 4456 5527 2549
Size: 254 1568 85 1071 3332
Fragments arranged by size:
3332 1568 1071 254 85
BsmI GAATG_Cn'
Cuts at: 3821 3914 3821
Size: 93 6217
BsmBI CGTCTCn'nnnn_
Cuts at: 3607 3607
Size: 6310
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2549 2803 2997 3282 3629 4371 4456
Size: 399 759 254 194 285 347 742 85
Cuts at: 4456 5527 1391
Size: 1071 2174
Fragments arranged by size:
2174 1071 759 742 399 347 285 254
194 85
BspEI T'CCGG_A
Cuts at: 5973 5973
Size: 6310
BspHI T'CATG_A
Cuts at: 4199 5929 4199
Size: 1730 4580
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2583 3665 844
Size: 1739 1082 3489
Fragments arranged by size:
3489 1739 1082
BsrBI CCG'CTC
Cuts at: 1394 1823 4197 1394
Size: 429 2374 3507
Fragments arranged by size:
3507 2374 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 2839 4805 4979 65
Size: 1183 1591 1966 174 1396
Fragments arranged by size:
1966 1591 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 3423 3519 4954 5141 1700
Size: 110 1613 96 1435 187 2869
Fragments arranged by size:
2869 1613 1435 187 110 96
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 6310
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 6310
BssSI C'ACGA_G
Cuts at: 4370 5382 4370
Size: 1012 5298
BstAPI GCAn_nnn'nTGC
Cuts at: 794 794
Size: 6310
BstBI TT'CG_AA
Cuts at: 5114 5114
Size: 6310
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 6310
BstXI CCAn_nnnn'nTGG
Cuts at: 3082 3082
Size: 6310
BstYI r'GATC_y
Cuts at: 1972 3204 3640 4007 4392 4409 5861 5947 5959
Size: 1232 436 367 385 17 1452 86 12
Cuts at: 5959 6305 1972
Size: 346 1977
Fragments arranged by size:
1977 1452 1232 436 385 367 346 86
17 12
BtgI C'CryG_G
Cuts at: 513 1065 2259 2602 513
Size: 552 1194 343 4221
Fragments arranged by size:
4221 1194 552 343
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 5571
Fragments arranged by size:
5571 683 56
BtsI GCAGTG_nn'
Cuts at: 2044 2778 2820 2878 3019 3353 3826 4620 4640
Size: 734 42 58 141 334 473 794 20
Cuts at: 4640 2044
Size: 3714
Fragments arranged by size:
3714 794 734 473 334 141 58 42
20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 6310
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 2754 2789 529
Size: 35 2190 35 4050
Fragments arranged by size:
4050 2190 35 35
DraI TTT'AAA
Cuts at: 3614 3614
Size: 6310
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 2092 5317 809
Size: 507 776 3225 1802
Fragments arranged by size:
3225 1802 776 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2256 3757 4646
Size: 54 1425 50 275 18 426 1501 889
Cuts at: 4646 6275 8
Size: 1629 43
Fragments arranged by size:
1629 1501 1425 889 426 275 54 50
43 18
EagI C'GGCC_G
Cuts at: 1537 1812 3757 1537
Size: 275 1945 4090
Fragments arranged by size:
4090 1945 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2169 2195 2205 2703 3121 3534 4240 1353
Size: 816 26 10 498 418 413 706 3423
Fragments arranged by size:
3423 816 706 498 418 413 26 10
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 4897 5271 5417 387
Size: 1064 138 3308 374 146 1280
Fragments arranged by size:
3308 1280 1064 374 146 138
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2055 2498 2510 2825 2840 3422 4373
Size: 393 423 443 12 315 15 582 951
Cuts at: 4373 4970 5757 6025 1239
Size: 597 787 268 1524
Fragments arranged by size:
1524 951 787 597 582 443 423 393
315 268 15 12
EcoRI G'AATT_C
Cuts at: 3619 3997 3619
Size: 378 5932
FspI TGC'GCA
Cuts at: 4816 4816
Size: 6310
HaeII r_GCGC'y
Cuts at: 1678 2194 5457 1678
Size: 516 3263 2531
Fragments arranged by size:
3263 2531 516
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2003 2035 2768 2800 3196
Size: 32 135 32 431 32 733 32 396
Cuts at: 3196 3228 3251 3283 4951 4983 5025 5057 1373
Size: 32 23 32 1668 32 42 32 2626
Fragments arranged by size:
2626 1668 733 431 396 135 42 32
32 32 32 32 32 32 32 23
HincII GTy'rAC
Cuts at: 669 1657 1938 3525 3654 3900 669
Size: 988 281 1587 129 246 3079
Fragments arranged by size:
3079 1587 988 281 246 129
HindIII A'AGCT_T
Cuts at: 748 748
Size: 6310
HpaI GTT'AAC
Cuts at: 3900 3900
Size: 6310
KpnI G_GTAC'C
Cuts at: 3635 3635
Size: 6310
MfeI C'AATT_G
Cuts at: 2372 3909 2372
Size: 1537 4773
MluI A'CGCG_T
Cuts at: 6186 6186
Size: 6310
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 1989 3323 3387 5424 5608 1305
Size: 684 1334 64 2037 184 2007
Fragments arranged by size:
2037 2007 1334 684 184 64
MscI TGG'CCA
Cuts at: 10 64 1489 1832 2258 10
Size: 54 1425 343 426 4062
Fragments arranged by size:
4062 1425 426 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2583 2759 4268 4627 4786 518
Size: 648 1417 176 1509 359 159 2042
Fragments arranged by size:
2042 1509 1417 648 359 176 159
MspA1I CmG'CkG
Cuts at: 1606 2123 3314 3490 3593 3726 4403 5551 5796
Size: 517 1191 176 103 133 677 1148 245
Cuts at: 5796 1606
Size: 2120
Fragments arranged by size:
2120 1191 1148 677 517 245 176 133
103
NcoI C'CATG_G
Cuts at: 513 1065 2259 2602 513
Size: 552 1194 343 4221
Fragments arranged by size:
4221 1194 552 343
NdeI CA'TA_TG
Cuts at: 387 2848 387
Size: 2461 3849
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 6310
NotI GC'GGCC_GC
Cuts at: 3757 3757
Size: 6310
NspI r_CATG'y
Cuts at: 1132 1229 2464 2797 3668 5213 1132
Size: 97 1235 333 871 1545 2229
Fragments arranged by size:
2229 1545 1235 871 333 97
PasI CC'CwG_GG
Cuts at: 1348 1348
Size: 6310
PciI A'CATG_T
Cuts at: 2460 5209 2460
Size: 2749 3561
PflMI CCAn_nnn'nTGG
Cuts at: 1108 2744 1108
Size: 1636 4674
PmeI GTTT'AAAC
Cuts at: 3614 3614
Size: 6310
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2288 2320 3120 3152 4379 4411 5892 5924 2288
Size: 32 800 32 1227 32 1481 32 2674
Fragments arranged by size:
2674 1481 1227 800 32 32 32 32
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 6310
PsiI TTA'TAA
Cuts at: 3880 3880
Size: 6310
PstI C_TGCA'G
Cuts at: 830 2334 2931 3118 3662 830
Size: 1504 597 187 544 3478
Fragments arranged by size:
3478 1504 597 544 187
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 6310
PvuII CAG'CTG
Cuts at: 2123 3314 3490 2123
Size: 1191 176 4943
Fragments arranged by size:
4943 1191 176
SalI G'TCGA_C
Cuts at: 1936 3652 1936
Size: 1716 4594
SapI GCTCTTCn'nnn_
Cuts at: 2703 2703
Size: 6310
SbfI CC_TGCA'GG
Cuts at: 3118 3662 3118
Size: 544 5766
ScaI AGT'ACT
Cuts at: 2031 4558 6270 2031
Size: 2527 1712 2071
Fragments arranged by size:
2527 2071 1712
SexAI A'CCwGG_T
Cuts at: 2233 2233
Size: 6310
SfcI C'TryA_G
Cuts at: 826 1045 2330 2927 3114 3658 4793 5474 5665
Size: 219 1285 597 187 544 1135 681 191
Cuts at: 5665 826
Size: 1471
Fragments arranged by size:
1471 1285 1135 681 597 544 219 191
187
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6310
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 6310
SmaI CCC'GGG
Cuts at: 1872 3637 1872
Size: 1765 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 4414 5315 5577 820
Size: 197 926 2471 901 262 1553
Fragments arranged by size:
2471 1553 926 901 262 197
SnaBI TAC'GTA
Cuts at: 493 4101 493
Size: 3608 2702
SpeI A'CTAG_T
Cuts at: 152 3089 152
Size: 2937 3373
SphI G_CATG'C
Cuts at: 1132 3668 1132
Size: 2536 3774
SspI AAT'ATT
Cuts at: 5 52 2408 4234 5
Size: 47 2356 1826 2081
Fragments arranged by size:
2356 2081 1826 47
StuI AGG'CCT
Cuts at: 1517 1517
Size: 6310
StyI C'CwwG_G
Cuts at: 513 1065 2259 2602 3749 513
Size: 552 1194 343 1147 3074
Fragments arranged by size:
3074 1194 1147 552 343
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 2991 4337 4354 4507 4692 5137 325
Size: 1255 1411 1346 17 153 185 445 1498
Fragments arranged by size:
1498 1411 1346 1255 445 185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 2029 3147 4556
Size: 274 80 33 51 1443 52 1118 1409
Cuts at: 4556 6268 96
Size: 1712 138
Fragments arranged by size:
1712 1443 1409 1118 274 138 80 52
51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 6270 6302 6270
Size: 32 6278
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 6310
XbaI T'CTAG_A
Cuts at: 2887 3061 3646 2887
Size: 174 585 5551
Fragments arranged by size:
5551 585 174
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 6310
XmaI C'CCGG_G
Cuts at: 1870 3635 1870
Size: 1765 4545
XmnI GAAnn'nnTTC
Cuts at: 2343 3135 3554 4439 2343
Size: 792 419 885 4214
Fragments arranged by size:
4214 885 792 419
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 4118 276
Size: 53 83 186 936 2584 2468
Fragments arranged by size:
2584 2468 936 186 83 53
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AflII AflIII AgeI
AhdI AleI AlfI AloI AlwNI ApaLI ApoI AseI
AsiSI AvaI BamHI BanI BanII BbsI BbvCI BcgI
BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI
BmtI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI
BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI
BsmBI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI
BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI
BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI
EagI EarI EciI Eco57MI EcoRI FspI HaeII Hin4I
HincII HindIII HpaI KpnI MfeI MluI MmeI MscI
MslI MspA1I NcoI NdeI NheI NotI NspI PasI
PciI PflMI PmeI PpiI PshAI PsiI PstI PvuI
PvuII SalI SapI SbfI ScaI SexAI SfcI SgfI
SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI
StyI TaqII TatI TstI Tth111I XbaI XhoI XmaI
XmnI ZraI
Enzymes that do not cut:
AarI AfeI ApaI AscI AvrII BaeI BbeI BfrBI
BplI BsiWI BstZ17I Bsu36I DraIII EcoICRI EcoNI EcoO109I
EcoRV FalI FseI FspAI KasI NaeI NarI NgoMIV
NruI NsiI PacI PfoI PmlI PpuMI PspOMI PspXI
PsrI RsrII SacI SacII SanDI SfiI SfoI SrfI
SwaI XcmI