(Circular) (Six-base) MAPSORT of: pFN21AB6681.seq Check: 8489 from: 1 to: 5986 pFN21AB6681 5986 bp With 182 enzymes: SgfI * October 20, 2009 10:58 .. AarI CACCTGCnnnn'nnnn_ Cuts at: 2758 2764 2785 2758 Size: 6 21 5959 Fragments arranged by size: 5959 21 6 AatII G_ACGT'C Cuts at: 278 331 414 600 1536 3796 278 Size: 53 83 186 936 2260 2468 Fragments arranged by size: 2468 2260 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 3329 1937 Size: 1392 4594 Acc65I G'GTAC_C Cuts at: 3307 3307 Size: 5986 AclI AA'CG_TT Cuts at: 1563 4113 4486 1563 Size: 2550 373 3063 Fragments arranged by size: 3063 2550 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 2477 2678 2708 3108 4049 5433 5701 1632 Size: 845 201 30 400 941 1384 268 1917 Fragments arranged by size: 1917 1384 941 845 400 268 201 30 AfeI AGC'GCT Cuts at: 2876 2876 Size: 5986 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 5789 AflIII A'CryG_T Cuts at: 1203 4885 5862 1203 Size: 3682 977 1327 Fragments arranged by size: 3682 1327 977 AgeI A'CCGG_T Cuts at: 4817 4817 Size: 5986 AhdI GACnn_n'nnGTC Cuts at: 4715 4715 Size: 5986 AleI CACnn'nnGTG Cuts at: 1166 3143 3242 1166 Size: 1977 99 3910 Fragments arranged by size: 3910 1977 99 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 1184 Size: 5986 AlwNI CAG_nnn'CTG Cuts at: 2201 2614 3729 5301 2201 Size: 413 1115 1572 2886 Fragments arranged by size: 2886 1572 1115 413 ApaI G_GGCC'C Cuts at: 2791 2992 2791 Size: 201 5785 ApaLI G'TGCA_C Cuts at: 4043 5199 4043 Size: 1156 4830 ApoI r'AATT_y Cuts at: 1493 2381 3295 3521 3673 5782 1493 Size: 888 914 226 152 2109 1697 Fragments arranged by size: 2109 1697 914 888 226 152 AseI AT'TA_AT Cuts at: 160 1032 4540 160 Size: 872 3508 1606 Fragments arranged by size: 3508 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 5986 AvaI C'yCGr_G Cuts at: 1870 1943 2847 2991 2997 3311 1870 Size: 73 904 144 6 314 4545 Fragments arranged by size: 4545 904 314 144 73 6 BamHI G'GATC_C Cuts at: 2568 3316 3683 2568 Size: 748 367 4871 Fragments arranged by size: 4871 748 367 BanI G'GyrC_C Cuts at: 618 943 1160 1787 3307 4762 618 Size: 325 217 627 1520 1455 1842 Fragments arranged by size: 1842 1520 1455 627 325 217 BanII G_rGCy'C Cuts at: 1391 2791 2992 2998 3305 1391 Size: 1400 201 6 307 4072 Fragments arranged by size: 4072 1400 307 201 6 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 2282 928 Size: 971 383 4632 Fragments arranged by size: 4632 971 383 BbvCI CC'TCA_GC Cuts at: 2970 2970 Size: 5986 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 4177 4211 1903 Size: 34 2240 34 3678 Fragments arranged by size: 3678 2240 34 34 BciVI GTATCCnnnnn_n' Cuts at: 3878 5088 3878 Size: 1210 4776 BclI T'GATC_A Cuts at: 1548 1548 Size: 5986 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 2339 2987 3026 Size: 107 122 71 631 605 667 648 39 Cuts at: 3026 4597 136 Size: 1571 1525 Fragments arranged by size: 1571 1525 667 648 631 605 122 107 71 39 BglII A'GATC_T Cuts at: 2361 5981 2361 Size: 3620 2366 BlpI GC'TnA_GC Cuts at: 3009 3060 3403 3009 Size: 51 343 5592 Fragments arranged by size: 5592 343 51 Bme1580I G_kGCm'C Cuts at: 1790 2791 2992 4047 5203 1790 Size: 1001 201 1055 1156 2573 Fragments arranged by size: 2573 1156 1055 1001 201 BmgBI CAC'GTC Cuts at: 1318 1318 Size: 5986 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 2060 3195 3717 Size: 416 53 83 399 24 643 1135 522 Cuts at: 3717 4675 442 Size: 958 1753 Fragments arranged by size: 1753 1135 958 643 522 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 5986 BplI GAGnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 2145 2145 Size: 5986 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2100 2327 2861 2906 4646 1239 Size: 861 227 534 45 1740 2579 Fragments arranged by size: 2579 1740 861 534 227 45 Bpu10I CC'TnA_GC Cuts at: 2970 2970 Size: 5986 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 2499 4111 4976 5274 2499 Size: 1612 865 298 3211 Fragments arranged by size: 3211 1612 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 2047 4649 882 Size: 617 548 2602 2219 Fragments arranged by size: 2602 2219 617 548 BsaAI yAC'GTr Cuts at: 493 1204 3777 493 Size: 711 2573 2702 Fragments arranged by size: 2702 2573 711 BsaBI GATnn'nnATC Cuts at: 5980 5980 Size: 5986 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 3793 4175 4810 275 Size: 53 83 186 936 2260 382 635 1451 Fragments arranged by size: 2260 1451 936 635 382 186 83 53 BsaWI w'CCGG_w Cuts at: 4419 4817 5091 5238 5649 4419 Size: 398 274 147 411 4756 Fragments arranged by size: 4756 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 2073 2103 1178 Size: 30 165 30 670 30 5061 Fragments arranged by size: 5061 670 165 30 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2372 2401 2768 3116 1186 Size: 1186 29 367 348 4056 Fragments arranged by size: 4056 1186 367 348 29 BseYI C'CCAG_C Cuts at: 2564 2790 5189 5685 2564 Size: 226 2399 496 2865 Fragments arranged by size: 2865 2399 496 226 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 2786 2792 2825 1163 Size: 573 1050 6 33 4324 Fragments arranged by size: 4324 1050 573 33 6 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 2253 3436 4197 5225 1540 Size: 275 186 252 1183 761 1028 2301 Fragments arranged by size: 2301 1183 1028 761 275 252 186 BsiHKAI G_wGCw'C Cuts at: 2701 4047 4132 5203 2701 Size: 1346 85 1071 3484 Fragments arranged by size: 3484 1346 1071 85 BsmI GAATG_Cn' Cuts at: 3497 3590 3497 Size: 93 5893 BsmBI CGTCTCn'nnnn_ Cuts at: 2303 2303 Size: 5986 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2701 2791 2992 2998 3305 4047 4132 Size: 399 911 90 201 6 307 742 85 Cuts at: 4132 5203 1391 Size: 1071 2174 Fragments arranged by size: 2174 1071 911 742 399 307 201 90 85 6 BspEI T'CCGG_A Cuts at: 5649 5649 Size: 5986 BspHI T'CATG_A Cuts at: 3875 5605 3875 Size: 1730 4256 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 2758 2764 2785 3341 844 Size: 1914 6 21 556 3489 Fragments arranged by size: 3489 1914 556 21 6 BsrBI CCG'CTC Cuts at: 1394 1823 2785 3873 1394 Size: 429 962 1088 3507 Fragments arranged by size: 3507 1088 962 429 BsrDI GCAATG_nn' Cuts at: 65 1248 2205 2298 4481 4655 65 Size: 1183 957 93 2183 174 1396 Fragments arranged by size: 2183 1396 1183 957 174 93 BsrFI r'CCGG_y Cuts at: 1700 1810 4630 4817 1700 Size: 110 2820 187 2869 Fragments arranged by size: 2869 2820 187 110 BsrGI T'GTAC_A Cuts at: 96 96 Size: 5986 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 5986 BssSI C'ACGA_G Cuts at: 4046 5058 4046 Size: 1012 4974 BstAPI GCAn_nnn'nTGC Cuts at: 794 2764 794 Size: 1970 4016 BstBI TT'CG_AA Cuts at: 4790 4790 Size: 5986 BstEII G'GTnAC_C Cuts at: 1184 1184 Size: 5986 BstYI r'GATC_y Cuts at: 1972 2115 2124 2361 2568 3316 3683 4068 4085 Size: 143 9 237 207 748 367 385 17 Cuts at: 4085 5537 5623 5635 5981 1972 Size: 1452 86 12 346 1977 Fragments arranged by size: 1977 1452 748 385 367 346 237 207 143 86 17 12 9 BtgI C'CryG_G Cuts at: 513 1065 2034 2179 3165 513 Size: 552 969 145 986 3334 Fragments arranged by size: 3334 986 969 552 145 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 487 Size: 683 56 5247 Fragments arranged by size: 5247 683 56 BtsI GCAGTG_nn' Cuts at: 2808 3163 3502 4296 4316 2808 Size: 355 339 794 20 4478 Fragments arranged by size: 4478 794 355 339 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 5986 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 5951 DraI TTT'AAA Cuts at: 3290 3290 Size: 5986 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 2681 4993 809 Size: 507 1365 2312 1802 Fragments arranged by size: 2312 1802 1365 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2250 2631 2730 Size: 54 1425 50 275 18 420 381 99 Cuts at: 2730 3018 3433 4322 5951 8 Size: 288 415 889 1629 43 Fragments arranged by size: 1629 1425 889 420 415 381 288 275 99 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 2250 3433 1537 Size: 275 438 1183 4090 Fragments arranged by size: 4090 1183 438 275 EarI CTCTTCn'nnn_ Cuts at: 1353 3916 1353 Size: 2563 3423 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 2945 4573 4947 5093 387 Size: 1064 138 1356 1628 374 146 1280 Fragments arranged by size: 1628 1356 1280 1064 374 146 138 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2100 2327 2477 2678 2708 2861 2906 Size: 393 468 227 150 201 30 153 45 Cuts at: 2906 3108 4049 4646 5433 5701 1239 Size: 202 941 597 787 268 1524 Fragments arranged by size: 1524 941 787 597 468 393 268 227 202 201 153 150 45 30 EcoNI CCTnn'n_nnAGG Cuts at: 2897 2897 Size: 5986 EcoO109I rG'GnC_Cy Cuts at: 2185 2988 2185 Size: 803 5183 EcoRI G'AATT_C Cuts at: 3295 3673 3295 Size: 378 5608 FspI TGC'GCA Cuts at: 4492 4492 Size: 5986 HaeII r_GCGC'y Cuts at: 1678 2878 2893 5133 1678 Size: 1200 15 2240 2531 Fragments arranged by size: 2531 2240 1200 15 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2073 2105 3097 3129 4627 Size: 32 135 32 501 32 992 32 1498 Cuts at: 4627 4659 4701 4733 1373 Size: 32 42 32 2626 Fragments arranged by size: 2626 1498 992 501 135 42 32 32 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 2425 2686 3330 3576 669 Size: 988 281 487 261 644 246 3079 Fragments arranged by size: 3079 988 644 487 281 261 246 HindIII A'AGCT_T Cuts at: 748 748 Size: 5986 HpaI GTT'AAC Cuts at: 3576 3576 Size: 5986 KpnI G_GTAC'C Cuts at: 3311 3311 Size: 5986 MfeI C'AATT_G Cuts at: 3585 3585 Size: 5986 MluI A'CGCG_T Cuts at: 5862 5862 Size: 5986 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 2134 5100 5284 1305 Size: 829 2966 184 2007 Fragments arranged by size: 2966 2007 829 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 2633 2732 3020 10 Size: 54 1425 343 801 99 288 2976 Fragments arranged by size: 2976 1425 801 343 288 99 54 MslI CAynn'nnrTG Cuts at: 518 1166 2351 2438 3143 3242 3944 4303 4462 Size: 648 1185 87 705 99 702 359 159 Cuts at: 4462 518 Size: 2042 Fragments arranged by size: 2042 1185 705 702 648 359 159 99 87 MspA1I CmG'CkG Cuts at: 1606 2761 2864 3043 3064 3153 3402 4079 5227 Size: 1155 103 179 21 89 249 677 1148 Cuts at: 5227 5472 1606 Size: 245 2120 Fragments arranged by size: 2120 1155 1148 677 249 245 179 103 89 21 NcoI C'CATG_G Cuts at: 513 1065 2034 2179 3165 513 Size: 552 969 145 986 3334 Fragments arranged by size: 3334 986 969 552 145 NdeI CA'TA_TG Cuts at: 387 387 Size: 5986 NheI G'CTAG_C Cuts at: 1052 1052 Size: 5986 NotI GC'GGCC_GC Cuts at: 2250 3433 2250 Size: 1183 4803 NspI r_CATG'y Cuts at: 1132 1229 3073 3344 4889 1132 Size: 97 1844 271 1545 2229 Fragments arranged by size: 2229 1844 1545 271 97 PasI CC'CwG_GG Cuts at: 1348 1348 Size: 5986 PciI A'CATG_T Cuts at: 4885 4885 Size: 5986 PflMI CCAn_nnn'nTGG Cuts at: 1108 2797 1108 Size: 1689 4297 PfoI T'CCnGG_A Cuts at: 2571 2571 Size: 5986 PmeI GTTT'AAAC Cuts at: 3290 3290 Size: 5986 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 4055 4087 5568 5600 4055 Size: 32 1481 32 4441 Fragments arranged by size: 4441 1481 32 32 PpuMI rG'GwC_Cy Cuts at: 2185 2185 Size: 5986 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 5986 PsiI TTA'TAA Cuts at: 3556 3556 Size: 5986 PspOMI G'GGCC_C Cuts at: 2787 2988 2787 Size: 201 5785 PstI C_TGCA'G Cuts at: 830 3338 830 Size: 2508 3478 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 5986 PvuII CAG'CTG Cuts at: 3043 3064 3043 Size: 21 5965 SalI G'TCGA_C Cuts at: 1936 3328 1936 Size: 1392 4594 SanDI GG'GwC_CC Cuts at: 2185 2185 Size: 5986 SbfI CC_TGCA'GG Cuts at: 3338 3338 Size: 5986 ScaI AGT'ACT Cuts at: 3195 4234 5946 3195 Size: 1039 1712 3235 Fragments arranged by size: 3235 1712 1039 SexAI A'CCwGG_T Cuts at: 2418 2418 Size: 5986 SfcI C'TryA_G Cuts at: 826 1045 3334 4469 5150 5341 826 Size: 219 2289 1135 681 191 1471 Fragments arranged by size: 2289 1471 1135 681 219 191 SfiI GGCCn_nnn'nGGCC Cuts at: 2339 2339 Size: 5986 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 5986 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 5986 SmaI CCC'GGG Cuts at: 1872 2849 3313 1872 Size: 977 464 4545 Fragments arranged by size: 4545 977 464 SmlI C'TyrA_G Cuts at: 820 1017 1943 2514 4090 4991 5253 820 Size: 197 926 571 1576 901 262 1553 Fragments arranged by size: 1576 1553 926 901 571 262 197 SnaBI TAC'GTA Cuts at: 493 3777 493 Size: 3284 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 5986 SphI G_CATG'C Cuts at: 1132 3344 1132 Size: 2212 3774 SspI AAT'ATT Cuts at: 5 52 3910 5 Size: 47 3858 2081 Fragments arranged by size: 3858 2081 47 StuI AGG'CCT Cuts at: 1517 2975 1517 Size: 1458 4528 StyI C'CwwG_G Cuts at: 513 1065 2034 2179 2634 3165 3425 513 Size: 552 969 145 455 531 260 3074 Fragments arranged by size: 3074 969 552 531 455 260 145 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 4013 4030 4183 4368 4813 325 Size: 1255 2433 17 153 185 445 1498 Fragments arranged by size: 2433 1498 1255 445 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 3193 4232 5944 Size: 274 80 33 51 1443 1216 1039 1712 Cuts at: 5944 96 Size: 138 Fragments arranged by size: 1712 1443 1216 1039 274 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 5946 5978 5946 Size: 32 5954 Tth111I GACn'n_nGTC Cuts at: 1316 2420 1316 Size: 1104 4882 XbaI T'CTAG_A Cuts at: 3322 3322 Size: 5986 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 5986 XmaI C'CCGG_G Cuts at: 1870 2847 3311 1870 Size: 977 464 4545 Fragments arranged by size: 4545 977 464 XmnI GAAnn'nnTTC Cuts at: 4115 4115 Size: 5986 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 3794 276 Size: 53 83 186 936 2260 2468 Fragments arranged by size: 2468 2260 936 186 83 53 Enzymes that do cut: AarI AatII AccI Acc65I AclI AcuI AfeI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbsI BbvCI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BplI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI BsmBI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstYI BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI EarI EciI Eco57MI EcoNI EcoO109I EcoRI FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I NcoI NdeI NheI NotI NspI PasI PciI PflMI PfoI PmeI PpiI PpuMI PshAI PsiI PspOMI PstI PvuI PvuII SalI SanDI SbfI ScaI SexAI SfcI SfiI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XhoI XmaI XmnI ZraI Enzymes that do not cut: AloI AscI AvrII BaeI BbeI BfrBI BsiWI BstXI BstZ17I Bsu36I DraIII EcoICRI EcoRV FalI FseI FspAI KasI NaeI NarI NgoMIV NruI NsiI PacI PmlI PspXI PsrI RsrII SacI SacII SapI SfoI SrfI SwaI XcmI