Restriction map for
pFN21AB6681
(Linear) (Six-base) MAPSORT of: KIBB6681.seq Check: 6466 from: 1 to: 1281
KIBB6681.orf
With 182 enzymes: SgfI *
October 7, 2009 14:22 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 0 753 759 780 1281
Size: 753 6 21 501
Fragments arranged by size:
753 501 21 6
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 472 673 703 1103 1281
Size: 472 201 30 400 178
Fragments arranged by size:
472 400 201 178 30
AfeI AGC'GCT
Cuts at: 0 871 1281
Size: 871 410
AleI CACnn'nnGTG
Cuts at: 0 1138 1237 1281
Size: 1138 99 44
Fragments arranged by size:
1138 99 44
AlwNI CAG_nnn'CTG
Cuts at: 0 196 609 1281
Size: 196 413 672
Fragments arranged by size:
672 413 196
ApaI G_GGCC'C
Cuts at: 0 786 987 1281
Size: 786 201 294
Fragments arranged by size:
786 294 201
ApoI r'AATT_y
Cuts at: 0 376 1281
Size: 376 905
AvaI C'yCGr_G
Cuts at: 0 842 986 992 1281
Size: 842 144 6 289
Fragments arranged by size:
842 289 144 6
BamHI G'GATC_C
Cuts at: 0 563 1281
Size: 563 718
BanII G_rGCy'C
Cuts at: 0 786 987 993 1281
Size: 786 201 6 288
Fragments arranged by size:
786 288 201 6
BbsI GAAGACnn'nnnn_
Cuts at: 0 277 1281
Size: 277 1004
BbvCI CC'TCA_GC
Cuts at: 0 965 1281
Size: 965 316
BglI GCCn_nnn'nGGC
Cuts at: 0 334 982 1021 1281
Size: 334 648 39 260
Fragments arranged by size:
648 334 260 39
BglII A'GATC_T
Cuts at: 0 356 1281
Size: 356 925
BlpI GC'TnA_GC
Cuts at: 0 1004 1055 1281
Size: 1004 51 226
Fragments arranged by size:
1004 226 51
Bme1580I G_kGCm'C
Cuts at: 0 786 987 1281
Size: 786 201 294
Fragments arranged by size:
786 294 201
BmrI ACTGGGnnnn_n'
Cuts at: 0 55 1190 1281
Size: 55 1135 91
Fragments arranged by size:
1135 91 55
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 140 1281
Size: 140 1141
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 95 322 856 901 1281
Size: 95 227 534 45 380
Fragments arranged by size:
534 380 227 95 45
Bpu10I CC'TnA_GC
Cuts at: 0 965 1281
Size: 965 316
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 494 1281
Size: 494 787
BsaI GGTCTCn'nnnn_
Cuts at: 0 42 1281
Size: 42 1239
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 68 98 1281
Size: 68 30 1183
Fragments arranged by size:
1183 68 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 367 396 763 1111 1281
Size: 367 29 367 348 170
Fragments arranged by size:
367 367 348 170 29
BseYI C'CCAG_C
Cuts at: 0 559 785 1281
Size: 559 226 496
Fragments arranged by size:
559 496 226
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 781 787 820 1281
Size: 781 6 33 461
Fragments arranged by size:
781 461 33 6
BsiEI CG_ry'CG
Cuts at: 0 248 1281
Size: 248 1033
BsiHKAI G_wGCw'C
Cuts at: 0 696 1281
Size: 696 585
BsmBI CGTCTCn'nnnn_
Cuts at: 0 298 1281
Size: 298 983
Bsp1286I G_dGCh'C
Cuts at: 0 696 786 987 993 1281
Size: 696 90 201 6 288
Fragments arranged by size:
696 288 201 90 6
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 753 759 780 1281
Size: 753 6 21 501
Fragments arranged by size:
753 501 21 6
BsrBI CCG'CTC
Cuts at: 0 780 1281
Size: 780 501
BsrDI GCAATG_nn'
Cuts at: 0 200 293 1281
Size: 200 93 988
Fragments arranged by size:
988 200 93
BstAPI GCAn_nnn'nTGC
Cuts at: 0 759 1281
Size: 759 522
BstYI r'GATC_y
Cuts at: 0 110 119 356 563 1281
Size: 110 9 237 207 718
Fragments arranged by size:
718 237 207 110 9
BtgI C'CryG_G
Cuts at: 0 29 174 1160 1281
Size: 29 145 986 121
Fragments arranged by size:
986 145 121 29
BtsI GCAGTG_nn'
Cuts at: 0 803 1158 1281
Size: 803 355 123
Fragments arranged by size:
803 355 123
DrdI GACnn_nn'nnGTC
Cuts at: 0 676 1281
Size: 676 605
EaeI y'GGCC_r
Cuts at: 0 245 626 725 1013 1281
Size: 245 381 99 288 268
Fragments arranged by size:
381 288 268 245 99
EagI C'GGCC_G
Cuts at: 0 245 1281
Size: 245 1036
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 0 940 1281
Size: 940 341
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 95 322 472 673 703 856 901 1103
Size: 95 227 150 201 30 153 45 202
Cuts at: 1103 1281
Size: 178
Fragments arranged by size:
227 202 201 178 153 150 95 45
30
EcoNI CCTnn'n_nnAGG
Cuts at: 0 892 1281
Size: 892 389
EcoO109I rG'GnC_Cy
Cuts at: 0 180 983 1281
Size: 180 803 298
Fragments arranged by size:
803 298 180
HaeII r_GCGC'y
Cuts at: 0 873 888 1281
Size: 873 15 393
Fragments arranged by size:
873 393 15
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 68 100 1092 1124 1281
Size: 68 32 992 32 157
Fragments arranged by size:
992 157 68 32 32
HincII GTy'rAC
Cuts at: 0 420 681 1281
Size: 420 261 600
Fragments arranged by size:
600 420 261
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 129 1281
Size: 129 1152
MscI TGG'CCA
Cuts at: 0 628 727 1015 1281
Size: 628 99 288 266
Fragments arranged by size:
628 288 266 99
MslI CAynn'nnrTG
Cuts at: 0 346 433 1138 1237 1281
Size: 346 87 705 99 44
Fragments arranged by size:
705 346 99 87 44
MspA1I CmG'CkG
Cuts at: 0 756 859 1038 1059 1148 1281
Size: 756 103 179 21 89 133
Fragments arranged by size:
756 179 133 103 89 21
NcoI C'CATG_G
Cuts at: 0 29 174 1160 1281
Size: 29 145 986 121
Fragments arranged by size:
986 145 121 29
NotI GC'GGCC_GC
Cuts at: 0 245 1281
Size: 245 1036
NspI r_CATG'y
Cuts at: 0 1068 1281
Size: 1068 213
PflMI CCAn_nnn'nTGG
Cuts at: 0 792 1281
Size: 792 489
PfoI T'CCnGG_A
Cuts at: 0 566 1281
Size: 566 715
PpuMI rG'GwC_Cy
Cuts at: 0 180 1281
Size: 180 1101
PspOMI G'GGCC_C
Cuts at: 0 782 983 1281
Size: 782 201 298
Fragments arranged by size:
782 298 201
PvuII CAG'CTG
Cuts at: 0 1038 1059 1281
Size: 1038 21 222
Fragments arranged by size:
1038 222 21
SanDI GG'GwC_CC
Cuts at: 0 180 1281
Size: 180 1101
ScaI AGT'ACT
Cuts at: 0 1190 1281
Size: 1190 91
SexAI A'CCwGG_T
Cuts at: 0 413 1281
Size: 413 868
SfiI GGCCn_nnn'nGGCC
Cuts at: 0 334 1281
Size: 334 947
SmaI CCC'GGG
Cuts at: 0 844 1281
Size: 844 437
SmlI C'TyrA_G
Cuts at: 0 509 1281
Size: 509 772
StuI AGG'CCT
Cuts at: 0 970 1281
Size: 970 311
StyI C'CwwG_G
Cuts at: 0 29 174 629 1160 1281
Size: 29 145 455 531 121
Fragments arranged by size:
531 455 145 121 29
TatI w'GTAC_w
Cuts at: 0 1188 1281
Size: 1188 93
Tth111I GACn'n_nGTC
Cuts at: 0 415 1281
Size: 415 866
XmaI C'CCGG_G
Cuts at: 0 842 1281
Size: 842 439
Enzymes that do cut:
AarI AcuI AfeI AleI AlwNI ApaI ApoI AvaI
BamHI BanII BbsI BbvCI BglI BglII BlpI Bme1580I
BmrI BplI BpmI Bpu10I BpuEI BsaI BsaXI BseRI
BseYI BsgI BsiEI BsiHKAI BsmBI Bsp1286I BspMI BsrBI
BsrDI BstAPI BstYI BtgI BtsI DrdI EaeI EagI
EciI Eco57MI EcoNI EcoO109I HaeII Hin4I HincII MmeI
MscI MslI MspA1I NcoI NotI NspI PflMI PfoI
PpuMI PspOMI PvuII SanDI ScaI SexAI SfiI SmaI
SmlI StuI StyI TatI Tth111I XmaI
Enzymes that do not cut:
AatII AccI Acc65I AclI AflII AflIII AgeI AhdI
AlfI AloI ApaLI AscI AseI AsiSI AvrII BaeI
BanI BbeI BcgI BciVI BclI BfrBI BmgBI BmtI
BsaAI BsaBI BsaHI BsaWI BsiWI BsmI BspEI BspHI
BsrFI BsrGI BssHII BssSI BstBI BstEII BstXI BstZ17I
Bsu36I BtgZI ClaI CspCI DraI DraIII EarI EcoICRI
EcoRI EcoRV FalI FseI FspI FspAI HindIII HpaI
KasI KpnI MfeI MluI NaeI NarI NdeI NgoMIV
NheI NruI NsiI PacI PasI PciI PmeI PmlI
PpiI PshAI PsiI PspXI PsrI PstI PvuI RsrII
SacI SacII SalI SapI SbfI SfcI SfoI SgfI
SgrAI SnaBI SpeI SphI SrfI SspI SwaI TaqII
TstI XbaI XcmI XhoI XmnI ZraI