(Circular) (Six-base) MAPSORT of: pFN21AE0696.seq Check: 4542 from: 1 to: 5917 pFN21AE0696 5917 bp With 182 enzymes: SgfI * October 20, 2009 10:50 .. AatII G_ACGT'C Cuts at: 278 331 414 600 1536 3727 278 Size: 53 83 186 936 2191 2468 Fragments arranged by size: 2468 2191 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 3180 3260 1937 Size: 1243 80 4594 Fragments arranged by size: 4594 1243 80 Acc65I G'GTAC_C Cuts at: 3238 3238 Size: 5917 AclI AA'CG_TT Cuts at: 1563 4044 4417 1563 Size: 2481 373 3063 Fragments arranged by size: 3063 2481 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 2847 2907 3980 5364 5632 1632 Size: 1215 60 1073 1384 268 1917 Fragments arranged by size: 1917 1384 1215 1073 268 60 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 5720 AflIII A'CryG_T Cuts at: 1203 2167 4816 5793 1203 Size: 964 2649 977 1327 Fragments arranged by size: 2649 1327 977 964 AgeI A'CCGG_T Cuts at: 4748 4748 Size: 5917 AhdI GACnn_n'nnGTC Cuts at: 4646 4646 Size: 5917 AleI CACnn'nnGTG Cuts at: 1166 1166 Size: 5917 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 1184 Size: 5917 AlwNI CAG_nnn'CTG Cuts at: 2858 3660 5232 2858 Size: 802 1572 3543 Fragments arranged by size: 3543 1572 802 ApaLI G'TGCA_C Cuts at: 3974 5130 3974 Size: 1156 4761 ApoI r'AATT_y Cuts at: 1493 2075 3226 3452 3604 5713 1493 Size: 582 1151 226 152 2109 1697 Fragments arranged by size: 2109 1697 1151 582 226 152 AseI AT'TA_AT Cuts at: 160 1032 4471 160 Size: 872 3439 1606 Fragments arranged by size: 3439 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 5917 AvaI C'yCGr_G Cuts at: 1870 1943 3242 1870 Size: 73 1299 4545 Fragments arranged by size: 4545 1299 73 BamHI G'GATC_C Cuts at: 3247 3614 3247 Size: 367 5550 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2861 3238 4693 618 Size: 325 217 627 1074 377 1455 1842 Fragments arranged by size: 1842 1455 1074 627 377 325 217 BanII G_rGCy'C Cuts at: 1391 3236 1391 Size: 1845 4072 BbeI G_GCGC'C Cuts at: 2865 2865 Size: 5917 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 2892 928 Size: 971 993 3953 Fragments arranged by size: 3953 993 971 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 1987 2021 3029 3063 4108 4142 1903 Size: 34 50 34 1008 34 1045 34 3678 Fragments arranged by size: 3678 1045 1008 50 34 34 34 34 BciVI GTATCCnnnnn_n' Cuts at: 2095 3809 5019 2095 Size: 1714 1210 2993 Fragments arranged by size: 2993 1714 1210 BclI T'GATC_A Cuts at: 1548 2290 1548 Size: 742 5175 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 4528 136 Size: 107 122 71 631 605 2856 1525 Fragments arranged by size: 2856 1525 631 605 122 107 71 BglII A'GATC_T Cuts at: 2891 5912 2891 Size: 3021 2896 BlpI GC'TnA_GC Cuts at: 3334 3334 Size: 5917 Bme1580I G_kGCm'C Cuts at: 1790 3978 5134 1790 Size: 2188 1156 2573 Fragments arranged by size: 2573 2188 1156 BmgBI CAC'GTC Cuts at: 1318 3214 1318 Size: 1896 4021 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 3648 4606 442 Size: 416 53 83 399 24 2231 958 1753 Fragments arranged by size: 2231 1753 958 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 5917 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 4577 1239 Size: 3338 2579 Bpu10I CC'TnA_GC Cuts at: 2222 2222 Size: 5917 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 2550 4042 4907 5205 2550 Size: 1492 865 298 3262 Fragments arranged by size: 3262 1492 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 3010 4580 882 Size: 617 1511 1570 2219 Fragments arranged by size: 2219 1570 1511 617 BsaAI yAC'GTr Cuts at: 493 1204 2168 3708 493 Size: 711 964 1540 2702 Fragments arranged by size: 2702 1540 964 711 BsaBI GATnn'nnATC Cuts at: 5911 5911 Size: 5917 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 2862 3724 4106 4741 Size: 53 83 186 936 1329 862 382 635 Cuts at: 4741 275 Size: 1451 Fragments arranged by size: 1451 1329 936 862 635 382 186 83 53 BsaWI w'CCGG_w Cuts at: 3202 4350 4748 5022 5169 5580 3202 Size: 1148 398 274 147 411 3539 Fragments arranged by size: 3539 1148 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 3073 3103 1178 Size: 30 165 30 1670 30 3992 Fragments arranged by size: 3992 1670 165 30 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 1186 Size: 5917 BseYI C'CCAG_C Cuts at: 5120 5616 5120 Size: 496 5421 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 2831 1163 Size: 573 1095 4249 Fragments arranged by size: 4249 1095 573 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 3367 4128 5156 1540 Size: 275 186 1366 761 1028 2301 Fragments arranged by size: 2301 1366 1028 761 275 186 BsiHKAI G_wGCw'C Cuts at: 3978 4063 5134 3978 Size: 85 1071 4761 Fragments arranged by size: 4761 1071 85 BsmI GAATG_Cn' Cuts at: 2260 3428 3521 2260 Size: 1168 93 4656 Fragments arranged by size: 4656 1168 93 BsmBI CGTCTCn'nnnn_ Cuts at: 2799 2799 Size: 5917 Bsp1286I G_dGCh'C Cuts at: 1391 1790 3236 3978 4063 5134 1391 Size: 399 1446 742 85 1071 2174 Fragments arranged by size: 2174 1446 1071 742 399 85 BspEI T'CCGG_A Cuts at: 5580 5580 Size: 5917 BspHI T'CATG_A Cuts at: 3806 5536 3806 Size: 1730 4187 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 3272 844 Size: 2428 3489 BsrBI CCG'CTC Cuts at: 1394 1823 3804 1394 Size: 429 1981 3507 Fragments arranged by size: 3507 1981 429 BsrDI GCAATG_nn' Cuts at: 65 1248 4412 4586 65 Size: 1183 3164 174 1396 Fragments arranged by size: 3164 1396 1183 174 BsrFI r'CCGG_y Cuts at: 1700 1810 4561 4748 1700 Size: 110 2751 187 2869 Fragments arranged by size: 2869 2751 187 110 BsrGI T'GTAC_A Cuts at: 96 96 Size: 5917 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 5917 BssSI C'ACGA_G Cuts at: 3977 4989 3977 Size: 1012 4905 BstAPI GCAn_nnn'nTGC Cuts at: 794 794 Size: 5917 BstBI TT'CG_AA Cuts at: 2083 4721 2083 Size: 2638 3279 BstEII G'GTnAC_C Cuts at: 1184 1184 Size: 5917 BstYI r'GATC_y Cuts at: 1972 2891 3247 3614 3999 4016 5468 5554 5566 Size: 919 356 367 385 17 1452 86 12 Cuts at: 5566 5912 1972 Size: 346 1977 Fragments arranged by size: 1977 1452 919 385 367 356 346 86 17 12 Bsu36I CC'TnA_GG Cuts at: 3156 3156 Size: 5917 BtgI C'CryG_G Cuts at: 513 1065 2395 3018 513 Size: 552 1330 623 3412 Fragments arranged by size: 3412 1330 623 552 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 2223 487 Size: 683 56 997 4181 Fragments arranged by size: 4181 997 683 56 BtsI GCAGTG_nn' Cuts at: 2619 3433 4227 4247 2619 Size: 814 794 20 4289 Fragments arranged by size: 4289 814 794 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 5917 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 5882 DraI TTT'AAA Cuts at: 3221 3221 Size: 5917 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 4924 809 Size: 507 3608 1802 Fragments arranged by size: 3608 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 3364 4253 5882 Size: 54 1425 50 275 18 1534 889 1629 Cuts at: 5882 8 Size: 43 Fragments arranged by size: 1629 1534 1425 889 275 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 3364 1537 Size: 275 1552 4090 Fragments arranged by size: 4090 1552 275 EarI CTCTTCn'nnn_ Cuts at: 1353 2624 2703 2823 3847 1353 Size: 1271 79 120 1024 3423 Fragments arranged by size: 3423 1271 1024 120 79 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 2249 4504 4878 5024 387 Size: 1064 138 660 2255 374 146 1280 Fragments arranged by size: 2255 1280 1064 660 374 146 138 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2847 2907 3980 4577 5364 5632 1239 Size: 393 1215 60 1073 597 787 268 1524 Fragments arranged by size: 1524 1215 1073 787 597 393 268 60 EcoO109I rG'GnC_Cy Cuts at: 2299 2882 2299 Size: 583 5334 EcoRI G'AATT_C Cuts at: 3226 3604 3226 Size: 378 5539 FspI TGC'GCA Cuts at: 4423 4423 Size: 5917 HaeII r_GCGC'y Cuts at: 1678 2865 5064 1678 Size: 1187 2199 2531 Fragments arranged by size: 2531 2199 1187 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 3073 3105 4558 4590 4632 Size: 32 135 32 1501 32 1453 32 42 Cuts at: 4632 4664 1373 Size: 32 2626 Fragments arranged by size: 2626 1501 1453 135 42 32 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 3261 3507 669 Size: 988 281 1323 246 3079 Fragments arranged by size: 3079 1323 988 281 246 HindIII A'AGCT_T Cuts at: 748 2183 748 Size: 1435 4482 HpaI GTT'AAC Cuts at: 3507 3507 Size: 5917 KasI G'GCGC_C Cuts at: 2861 2861 Size: 5917 KpnI G_GTAC'C Cuts at: 3242 3242 Size: 5917 MfeI C'AATT_G Cuts at: 2645 3516 2645 Size: 871 5046 MluI A'CGCG_T Cuts at: 5793 5793 Size: 5917 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 2152 2822 5031 5215 1305 Size: 847 670 2209 184 2007 Fragments arranged by size: 2209 2007 847 670 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 10 Size: 54 1425 343 4095 Fragments arranged by size: 4095 1425 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 2759 3875 4234 4393 518 Size: 648 1593 1116 359 159 2042 Fragments arranged by size: 2042 1593 1116 648 359 159 MspA1I CmG'CkG Cuts at: 1606 2878 3333 4010 5158 5403 1606 Size: 1272 455 677 1148 245 2120 Fragments arranged by size: 2120 1272 1148 677 455 245 NarI GG'CG_CC Cuts at: 2862 2862 Size: 5917 NcoI C'CATG_G Cuts at: 513 1065 2395 3018 513 Size: 552 1330 623 3412 Fragments arranged by size: 3412 1330 623 552 NdeI CA'TA_TG Cuts at: 387 387 Size: 5917 NheI G'CTAG_C Cuts at: 1052 1052 Size: 5917 NotI GC'GGCC_GC Cuts at: 3364 3364 Size: 5917 NspI r_CATG'y Cuts at: 1132 1229 3275 4820 1132 Size: 97 2046 1545 2229 Fragments arranged by size: 2229 2046 1545 97 PasI CC'CwG_GG Cuts at: 1348 1348 Size: 5917 PciI A'CATG_T Cuts at: 4816 4816 Size: 5917 PflMI CCAn_nnn'nTGG Cuts at: 1108 1108 Size: 5917 PfoI T'CCnGG_A Cuts at: 2733 2733 Size: 5917 PmeI GTTT'AAAC Cuts at: 3221 3221 Size: 5917 PmlI CAC'GTG Cuts at: 2168 2168 Size: 5917 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 3986 4018 5499 5531 3986 Size: 32 1481 32 4372 Fragments arranged by size: 4372 1481 32 32 PpuMI rG'GwC_Cy Cuts at: 2299 2882 2299 Size: 583 5334 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 5917 PsiI TTA'TAA Cuts at: 3487 3487 Size: 5917 PstI C_TGCA'G Cuts at: 830 3269 830 Size: 2439 3478 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 5917 SalI G'TCGA_C Cuts at: 1936 3259 1936 Size: 1323 4594 SanDI GG'GwC_CC Cuts at: 2882 2882 Size: 5917 SbfI CC_TGCA'GG Cuts at: 3269 3269 Size: 5917 ScaI AGT'ACT Cuts at: 2273 4165 5877 2273 Size: 1892 1712 2313 Fragments arranged by size: 2313 1892 1712 SfcI C'TryA_G Cuts at: 826 1045 3265 4400 5081 5272 826 Size: 219 2220 1135 681 191 1471 Fragments arranged by size: 2220 1471 1135 681 219 191 SfoI GGC'GCC Cuts at: 2863 2863 Size: 5917 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 5917 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 5917 SmaI CCC'GGG Cuts at: 1872 3244 1872 Size: 1372 4545 SmlI C'TyrA_G Cuts at: 820 1017 1943 2565 4021 4922 5184 820 Size: 197 926 622 1456 901 262 1553 Fragments arranged by size: 1553 1456 926 901 622 262 197 SnaBI TAC'GTA Cuts at: 493 3708 493 Size: 3215 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 5917 SphI G_CATG'C Cuts at: 1132 3275 1132 Size: 2143 3774 SspI AAT'ATT Cuts at: 5 52 3841 5 Size: 47 3789 2081 Fragments arranged by size: 3789 2081 47 StuI AGG'CCT Cuts at: 1517 1517 Size: 5917 StyI C'CwwG_G Cuts at: 513 1065 2395 3018 3051 3356 513 Size: 552 1330 623 33 305 3074 Fragments arranged by size: 3074 1330 623 552 305 33 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 3944 3961 4114 4299 4744 325 Size: 1255 2364 17 153 185 445 1498 Fragments arranged by size: 2364 1498 1255 445 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 2271 4163 5875 Size: 274 80 33 51 1443 294 1892 1712 Cuts at: 5875 96 Size: 138 Fragments arranged by size: 1892 1712 1443 294 274 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 5877 5909 5877 Size: 32 5885 Tth111I GACn'n_nGTC Cuts at: 1316 1316 Size: 5917 XbaI T'CTAG_A Cuts at: 2044 3253 2044 Size: 1209 4708 XcmI CCAnnnn_n'nnnnTGG Cuts at: 2011 2402 2011 Size: 391 5526 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 5917 XmaI C'CCGG_G Cuts at: 1870 3242 1870 Size: 1372 4545 XmnI GAAnn'nnTTC Cuts at: 2273 2697 4046 2273 Size: 424 1349 4144 Fragments arranged by size: 4144 1349 424 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 3725 276 Size: 53 83 186 936 2191 2468 Fragments arranged by size: 2468 2191 936 186 83 53 Enzymes that do cut: AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbeI BbsI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI BsmBI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstYI Bsu36I BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI EarI EciI Eco57MI EcoO109I EcoRI FspI HaeII Hin4I HincII HindIII HpaI KasI KpnI MfeI MluI MmeI MscI MslI MspA1I NarI NcoI NdeI NheI NotI NspI PasI PciI PflMI PfoI PmeI PmlI PpiI PpuMI PshAI PsiI PstI PvuI SalI SanDI SbfI ScaI SfcI SfoI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI Enzymes that do not cut: AarI AfeI AloI ApaI AscI AvrII BaeI BbvCI BfrBI BplI BsiWI BstXI BstZ17I DraIII EcoICRI EcoNI EcoRV FalI FseI FspAI NaeI NgoMIV NruI NsiI PacI PspOMI PspXI PsrI PvuII RsrII SacI SacII SapI SexAI SfiI SrfI SwaI