(Circular) (Six-base) MAPSORT of: pFN21AE6125.seq Check: 9423 from: 1 to: 5233 pFN21AE6125 5233 bp With 182 enzymes: SgfI * December 8, 2014 13:52 .. AatII G_ACGT'C Cuts at: 278 331 414 600 1536 3043 278 Size: 53 83 186 936 1507 2468 Fragments arranged by size: 2468 1507 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 2576 1937 Size: 639 4594 Acc65I G'GTAC_C Cuts at: 2554 2554 Size: 5233 AclI AA'CG_TT Cuts at: 1563 3360 3733 1563 Size: 1797 373 3063 Fragments arranged by size: 3063 1797 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 2282 3296 4680 4948 1632 Size: 650 1014 1384 268 1917 Fragments arranged by size: 1917 1384 1014 650 268 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 5036 AflIII A'CryG_T Cuts at: 1203 4132 5109 1203 Size: 2929 977 1327 Fragments arranged by size: 2929 1327 977 AgeI A'CCGG_T Cuts at: 4064 4064 Size: 5233 AhdI GACnn_n'nnGTC Cuts at: 3962 3962 Size: 5233 AleI CACnn'nnGTG Cuts at: 1166 2249 1166 Size: 1083 4150 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 1184 Size: 5233 AlwNI CAG_nnn'CTG Cuts at: 2065 2113 2976 4548 2065 Size: 48 863 1572 2750 Fragments arranged by size: 2750 1572 863 48 ApaI G_GGCC'C Cuts at: 2035 2195 2035 Size: 160 5073 ApaLI G'TGCA_C Cuts at: 3290 4446 3290 Size: 1156 4077 ApoI r'AATT_y Cuts at: 1493 2542 2768 2920 5029 1493 Size: 1049 226 152 2109 1697 Fragments arranged by size: 2109 1697 1049 226 152 AseI AT'TA_AT Cuts at: 160 1032 3787 160 Size: 872 2755 1606 Fragments arranged by size: 2755 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 5233 AvaI C'yCGr_G Cuts at: 1870 1943 2558 1870 Size: 73 615 4545 Fragments arranged by size: 4545 615 73 BamHI G'GATC_C Cuts at: 2563 2930 2563 Size: 367 4866 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2240 2554 4009 618 Size: 325 217 627 453 314 1455 1842 Fragments arranged by size: 1842 1455 627 453 325 314 217 BanII G_rGCy'C Cuts at: 1391 2035 2195 2552 1391 Size: 644 160 357 4072 Fragments arranged by size: 4072 644 357 160 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 928 Size: 971 4262 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 3424 3458 1903 Size: 34 1487 34 3678 Fragments arranged by size: 3678 1487 34 34 BciVI GTATCCnnnnn_n' Cuts at: 3125 4335 3125 Size: 1210 4023 BclI T'GATC_A Cuts at: 1548 1548 Size: 5233 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 3844 136 Size: 107 122 71 631 605 2172 1525 Fragments arranged by size: 2172 1525 631 605 122 107 71 BglII A'GATC_T Cuts at: 5228 5228 Size: 5233 BlpI GC'TnA_GC Cuts at: 2650 2650 Size: 5233 Bme1580I G_kGCm'C Cuts at: 1790 2035 2195 2335 3294 4450 1790 Size: 245 160 140 959 1156 2573 Fragments arranged by size: 2573 1156 959 245 160 140 BmgBI CAC'GTC Cuts at: 1318 1318 Size: 5233 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 2371 2964 3922 Size: 416 53 83 399 24 954 593 958 Cuts at: 3922 442 Size: 1753 Fragments arranged by size: 1753 958 954 593 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 5233 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 3893 1239 Size: 2654 2579 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 3358 4223 4521 3358 Size: 865 298 4070 Fragments arranged by size: 4070 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 3896 882 Size: 617 2397 2219 Fragments arranged by size: 2397 2219 617 BsaAI yAC'GTr Cuts at: 493 1204 3024 493 Size: 711 1820 2702 Fragments arranged by size: 2702 1820 711 BsaBI GATnn'nnATC Cuts at: 5227 5227 Size: 5233 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 3040 3422 4057 275 Size: 53 83 186 936 1507 382 635 1451 Fragments arranged by size: 1507 1451 936 635 382 186 83 53 BsaWI w'CCGG_w Cuts at: 3666 4064 4338 4485 4896 3666 Size: 398 274 147 411 4003 Fragments arranged by size: 4003 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 1178 Size: 30 165 30 5008 Fragments arranged by size: 5008 165 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 1186 Size: 5233 BseYI C'CCAG_C Cuts at: 2072 4436 4932 2072 Size: 2364 496 2373 Fragments arranged by size: 2373 2364 496 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 2447 1163 Size: 573 711 3949 Fragments arranged by size: 3949 711 573 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 2683 3444 4472 1540 Size: 275 186 682 761 1028 2301 Fragments arranged by size: 2301 1028 761 682 275 186 BsiHKAI G_wGCw'C Cuts at: 3294 3379 4450 3294 Size: 85 1071 4077 Fragments arranged by size: 4077 1071 85 BsmI GAATG_Cn' Cuts at: 2744 2837 2744 Size: 93 5140 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2035 2195 2335 2552 3294 3379 4450 Size: 399 245 160 140 217 742 85 1071 Cuts at: 4450 1391 Size: 2174 Fragments arranged by size: 2174 1071 742 399 245 217 160 140 85 BspEI T'CCGG_A Cuts at: 4896 4896 Size: 5233 BspHI T'CATG_A Cuts at: 3122 4852 3122 Size: 1730 3503 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 2588 844 Size: 1744 3489 BsrBI CCG'CTC Cuts at: 1394 1823 2042 3120 1394 Size: 429 219 1078 3507 Fragments arranged by size: 3507 1078 429 219 BsrDI GCAATG_nn' Cuts at: 65 1248 3728 3902 65 Size: 1183 2480 174 1396 Fragments arranged by size: 2480 1396 1183 174 BsrFI r'CCGG_y Cuts at: 1700 1810 3877 4064 1700 Size: 110 2067 187 2869 Fragments arranged by size: 2869 2067 187 110 BsrGI T'GTAC_A Cuts at: 96 96 Size: 5233 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 5233 BssSI C'ACGA_G Cuts at: 3293 4305 3293 Size: 1012 4221 BstAPI GCAn_nnn'nTGC Cuts at: 794 794 Size: 5233 BstBI TT'CG_AA Cuts at: 4037 4037 Size: 5233 BstEII G'GTnAC_C Cuts at: 1184 1184 Size: 5233 BstXI CCAn_nnnn'nTGG Cuts at: 2251 2251 Size: 5233 BstYI r'GATC_y Cuts at: 1972 2563 2930 3315 3332 4784 4870 4882 5228 Size: 591 367 385 17 1452 86 12 346 Cuts at: 5228 1972 Size: 1977 Fragments arranged by size: 1977 1452 591 385 367 346 86 17 12 BtgI C'CryG_G Cuts at: 513 1065 2004 2048 2163 513 Size: 552 939 44 115 3583 Fragments arranged by size: 3583 939 552 115 44 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 487 Size: 683 56 4494 Fragments arranged by size: 4494 683 56 BtsI GCAGTG_nn' Cuts at: 2749 3543 3563 2749 Size: 794 20 4419 Fragments arranged by size: 4419 794 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 5233 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 5198 DraI TTT'AAA Cuts at: 2537 2537 Size: 5233 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 2074 2167 4240 809 Size: 507 758 93 2073 1802 Fragments arranged by size: 2073 1802 758 507 93 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2037 2469 2680 Size: 54 1425 50 275 18 207 432 211 Cuts at: 2680 3569 5198 8 Size: 889 1629 43 Fragments arranged by size: 1629 1425 889 432 275 211 207 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 2680 1537 Size: 275 868 4090 Fragments arranged by size: 4090 868 275 EarI CTCTTCn'nnn_ Cuts at: 1353 3163 1353 Size: 1810 3423 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 3820 4194 4340 387 Size: 1064 138 2231 374 146 1280 Fragments arranged by size: 2231 1280 1064 374 146 138 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2282 3296 3893 4680 4948 1239 Size: 393 650 1014 597 787 268 1524 Fragments arranged by size: 1524 1014 787 650 597 393 268 EcoNI CCTnn'n_nnAGG Cuts at: 2199 2199 Size: 5233 EcoO109I rG'GnC_Cy Cuts at: 2032 2062 2076 2192 2032 Size: 30 14 116 5073 Fragments arranged by size: 5073 116 30 14 EcoRI G'AATT_C Cuts at: 2542 2920 2542 Size: 378 4855 FspI TGC'GCA Cuts at: 3739 3739 Size: 5233 HaeII r_GCGC'y Cuts at: 1678 4380 1678 Size: 2702 2531 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2087 2113 2119 2145 3874 Size: 32 135 32 515 26 6 26 1729 Cuts at: 3874 3906 3948 3980 1373 Size: 32 42 32 2626 Fragments arranged by size: 2626 1729 515 135 42 32 32 32 32 26 26 6 HincII GTy'rAC Cuts at: 669 1657 1938 2577 2823 669 Size: 988 281 639 246 3079 Fragments arranged by size: 3079 988 639 281 246 HindIII A'AGCT_T Cuts at: 748 748 Size: 5233 HpaI GTT'AAC Cuts at: 2823 2823 Size: 5233 KpnI G_GTAC'C Cuts at: 2558 2558 Size: 5233 MfeI C'AATT_G Cuts at: 2832 2832 Size: 5233 MluI A'CGCG_T Cuts at: 5109 5109 Size: 5233 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 2256 4347 4531 1305 Size: 951 2091 184 2007 Fragments arranged by size: 2091 2007 951 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 10 Size: 54 1425 343 3411 Fragments arranged by size: 3411 1425 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 2249 3191 3550 3709 518 Size: 648 1083 942 359 159 2042 Fragments arranged by size: 2042 1083 942 648 359 159 MspA1I CmG'CkG Cuts at: 1606 2029 2072 2113 2377 2649 3326 4474 4719 Size: 423 43 41 264 272 677 1148 245 Cuts at: 4719 1606 Size: 2120 Fragments arranged by size: 2120 1148 677 423 272 264 245 43 41 NcoI C'CATG_G Cuts at: 513 1065 2004 2163 513 Size: 552 939 159 3583 Fragments arranged by size: 3583 939 552 159 NdeI CA'TA_TG Cuts at: 387 387 Size: 5233 NheI G'CTAG_C Cuts at: 1052 1052 Size: 5233 NotI GC'GGCC_GC Cuts at: 2680 2680 Size: 5233 NspI r_CATG'y Cuts at: 1132 1229 2591 4136 1132 Size: 97 1362 1545 2229 Fragments arranged by size: 2229 1545 1362 97 PasI CC'CwG_GG Cuts at: 1348 2173 1348 Size: 825 4408 PciI A'CATG_T Cuts at: 4132 4132 Size: 5233 PflMI CCAn_nnn'nTGG Cuts at: 1108 1108 Size: 5233 PfoI T'CCnGG_A Cuts at: 2513 2513 Size: 5233 PmeI GTTT'AAAC Cuts at: 2537 2537 Size: 5233 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 3302 3334 4815 4847 3302 Size: 32 1481 32 3688 Fragments arranged by size: 3688 1481 32 32 PpuMI rG'GwC_Cy Cuts at: 2062 2076 2062 Size: 14 5219 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 5233 PsiI TTA'TAA Cuts at: 2803 2803 Size: 5233 PspOMI G'GGCC_C Cuts at: 2031 2191 2031 Size: 160 5073 PstI C_TGCA'G Cuts at: 830 2136 2585 830 Size: 1306 449 3478 Fragments arranged by size: 3478 1306 449 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 5233 PvuII CAG'CTG Cuts at: 2072 2113 2072 Size: 41 5192 SalI G'TCGA_C Cuts at: 1936 2575 1936 Size: 639 4594 SanDI GG'GwC_CC Cuts at: 2076 2076 Size: 5233 SbfI CC_TGCA'GG Cuts at: 2585 2585 Size: 5233 ScaI AGT'ACT Cuts at: 3481 5193 3481 Size: 1712 3521 SexAI A'CCwGG_T Cuts at: 2445 2445 Size: 5233 SfcI C'TryA_G Cuts at: 826 1045 2132 2372 2581 3716 4397 4588 826 Size: 219 1087 240 209 1135 681 191 1471 Fragments arranged by size: 1471 1135 1087 681 240 219 209 191 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 5233 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 5233 SmaI CCC'GGG Cuts at: 1872 2560 1872 Size: 688 4545 SmlI C'TyrA_G Cuts at: 820 1017 1943 3337 4238 4500 820 Size: 197 926 1394 901 262 1553 Fragments arranged by size: 1553 1394 926 901 262 197 SnaBI TAC'GTA Cuts at: 493 3024 493 Size: 2531 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 5233 SphI G_CATG'C Cuts at: 1132 2591 1132 Size: 1459 3774 SspI AAT'ATT Cuts at: 5 52 3157 5 Size: 47 3105 2081 Fragments arranged by size: 3105 2081 47 StuI AGG'CCT Cuts at: 1517 1517 Size: 5233 StyI C'CwwG_G Cuts at: 513 1065 2004 2163 2672 513 Size: 552 939 159 509 3074 Fragments arranged by size: 3074 939 552 509 159 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 2081 3260 3277 3430 3615 4060 325 Size: 1255 501 1179 17 153 185 445 1498 Fragments arranged by size: 1498 1255 1179 501 445 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 2056 3479 5191 Size: 274 80 33 51 1443 79 1423 1712 Cuts at: 5191 96 Size: 138 Fragments arranged by size: 1712 1443 1423 274 138 80 79 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 5193 5225 5193 Size: 32 5201 Tth111I GACn'n_nGTC Cuts at: 1316 1316 Size: 5233 XbaI T'CTAG_A Cuts at: 2569 2569 Size: 5233 XcmI CCAnnnn_n'nnnnTGG Cuts at: 2170 2443 2170 Size: 273 4960 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 5233 XmaI C'CCGG_G Cuts at: 1870 2558 1870 Size: 688 4545 XmnI GAAnn'nnTTC Cuts at: 3362 3362 Size: 5233 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 3041 276 Size: 53 83 186 936 1507 2468 Fragments arranged by size: 2468 1507 936 186 83 53 Enzymes that do cut: AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbsI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BpmI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI EarI EciI Eco57MI EcoNI EcoO109I EcoRI FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I NcoI NdeI NheI NotI NspI PasI PciI PflMI PfoI PmeI PpiI PpuMI PshAI PsiI PspOMI PstI PvuI PvuII SalI SanDI SbfI ScaI SexAI SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI Enzymes that do not cut: AarI AfeI AloI AscI AvrII BaeI BbeI BbvCI BfrBI BplI Bpu10I BsiWI BsmBI BstZ17I Bsu36I DraIII EcoICRI EcoRV FalI FseI FspAI KasI NaeI NarI NgoMIV NruI NsiI PacI PmlI PspXI PsrI RsrII SacI SacII SapI SfiI SfoI SrfI SwaI