################################## # NO CUT ENZYMES ( 110 enzymes) # ################################## AatII Acc65I AccI AccIII AclI AflII AgeI AhdI Alw44I Aor51HI ApaI ApaLI AscI AseI AsnI Asp700I Asp718I AspEI AspI AviII AvrII BamHI BbrPI BcgI BciVI BclI BfrI BlnI BlpI Bpu1102I BsaBI BsaWI BseAI BsiWI BspDI BspEI BspHI BspMI BsrBI BsrDI BssHII BssSI Bst1107I BstBI BstXI BstZ17I CelII ClaI CpoI DraI DrdI DsaI EagI Eam1105I EclXI Eco47III Eco52I EcoT22I FbaI FseI FspI HincII HindII HindIII HpaI KpnI KspI MamI MfeI MluI MroI MunI NcoI NdeI NheI NotI NruI NsiI NspV PacI PflFI PinAI PmaCI PmeI PmlI PshAI PshBI Psp1406I RcaI RsrII SacI SacII SalI SbfI ScaI SfuI SgrAI SmaI SnaBI SpeI SphI Sse8387I SspI SwaI Tth111I XbaI XcmI XmaCI XmaI XmnI ################################### # ONE CUT ENZYMES ( 50 enzymes) # ################################### AcsI 1368 GAATTC [GA]^AATT[CT] AcyI 329 GGCGCC G[GA]^CG[CT]C AocI 187 CCTGAGG CC^T[ACGT]AGG ApoI 1368 GAATTC [GA]^AATT[CT] BanII 972 GGGCTC G[GA]GC[CT]^C BbeI 329 GGCGCC GGCGC^C BbsI 955 GTCTTC GAAGAC(2/6) BglII 143 AGATCT A^GATCT BpuAI 955 GTCTTC GAAGAC(2/6) BsaHI 329 GGCGCC G[GA]^CG[CT]C BsaI 1051 GGTCTC GGTCTC(1/5) BsiEI 783 CGATCG CG[GA][CT]^CG BsmBI 829 GAGACG CGTCTC(1/5) BsmI 742 GCATTC GAATGC(1/-1) Bsp1407I 701 TGTACA T^GTACA BspLU11I 448 ACATGT A^CATGT BsrGI 701 TGTACA T^GTACA BsrI 864 ACTGG ACTGG(1/-1) BstAPI 862 GCACTGGATGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC BstEII 649 GGTGACC G^GT[ACGT]ACC BstPI 649 GGTGACC G^GT[ACGT]ACC Bsu36I 187 CCTGAGG CC^T[ACGT]AGG DraIII 1009 CACATGGTG CAC[ACGT][ACGT][ACGT]^GTG EarI 835 CTCTTC CTCTTC(1/4) Eco81I 187 CCTGAGG CC^T[ACGT]AGG EcoNI 333 CCTGAAGGAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG EcoO65I 649 GGTGACC G^GT[ACGT]ACC EcoRI 1368 GAATTC G^AATTC EcoRV 1134 GATATC GAT^ATC HaeII 329 GGCGCC [GA]GCGC^[CT] Hin1I 329 GGCGCC G[GA]^CG[CT]C KasI 329 GGCGCC G^GCGCC Ksp632I 835 CTCTTC CTCTTC(1/4) MseI 512 TTAA T^TAA MslI 718 CACCGGGATG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG NarI 329 GGCGCC GG^CGCC PaeR7I 554 CTCGAG C^TCGAG PflMI 1411 CCAGCTGCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG PpuMI 1397 GGGTCCC [GA]G^G[AT]CC[CT] PvuI 783 CGATCG CGAT^CG SapI 834 GCTCTTC GCTCTTC(1/4) SexAI 317 ACCAGGT A^CC[AT]GGT SfiI 39 GGCCTCTGGGGCC GGCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGCC SfoI 329 GGCGCC GGC^GCC SspBI 701 TGTACA T^GTACA StuI 1113 AGGCCT AGG^CCT Tru9I 512 TTAA T^TAA Tsp509I 1369 AATT ^AATT Van91I 1411 CCAGCTGCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG XhoI 554 CTCGAG C^TCGAG ################################### # TWO CUT ENZYMES ( 29 enzymes) # ################################### AflIII 448 ACATGT A^C[GA][CT]GT 921 ACGTGT AlwI 390 GATCC GGATC(4/5) 933 GATCC AspHI 1006 GAGCAC G[AT]GC[AT]^C 1327 GAGCAC AvaI 554 CTCGAG C^[CT]CG[GA]G 937 CCCGAG AvaII 315 GGACC G^G[AT]CC 1398 GGTCC BbvCI 404 CCTCAGC CCTCAGC(-5/-2) 292 GCTGAGG BfaI 172 CTAG C^TAG 259 CTAG BglI 40 GCCTCTGGGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC 965 GCCACCAGGGC BsaAI 823 TACGTG [CT]AC^GT[GA] 920 TACGTG BsgI 221 CTGCAC GTGCAG(16/14) 860 CTGCAC BsiHKAI 1006 GAGCAC G[AT]GC[AT]^C 1327 GAGCAC BsmAI 829 GAGAC GTCTC(1/5) 1052 GTCTC BsoBI 554 CTCGAG C^[CT]CG[GA]G 937 CCCGAG BsrFI 36 GCCGGC [GA]^CCGG[CT] 67 GCCGGC Cfr10I 36 GCCGGC [GA]^CCGG[CT] 67 GCCGGC DraII 395 AGGCCCC [GA]G^G[ACGT]CC[CT] 1397 GGGTCCC EcoO109I 395 AGGCCCC [GA]G^G[ACGT]CC[CT] 1397 GGGTCCC EcoT14I 116 CCAAGG C^C[AT][AT]GG 178 CCAAGG HgaI 831 GACGC GACGC(5/10) 850 GACGC HphI 649 GGTGA GGTGA(8/7) 461 TCACC MaeI 172 CTAG C^TAG 259 CTAG NaeI 36 GCCGGC GCC^GGC 67 GCCGGC NgoMIV 36 GCCGGC G^CCGGC 67 GCCGGC NspI 448 ACATGT [GA]CATG^[CT] 543 GCATGT PvuII 407 CAGCTG CAG^CTG 1412 CAGCTG StyI 116 CCAAGG C^C[AT][AT]GG 178 CCAAGG TaqI 555 TCGA T^CGA 980 TCGA TfiI 549 GATTC G^A[AT]TC 1352 GAATC TthHB8I 555 TCGA T^CGA 980 TCGA ######################################### # 3 OR MORE CUT ENZYMES ( 69 enzymes) # ######################################### AccII AciI AfaI AluI AlwNI BalI BanI BbvI BcnI BmyI BpmI BsaJI BseRI BsiYI BslI BsmFI Bsp1286I BssKI BstF5I BstNI BstUI BstYI Cac8I CfoI Cfr13I DdeI DpnII EaeI EcoRII FauI Fnu4HI FokI HaeIII HapII HhaI HinP1I HinfI HpaII ItaI MaeII MaeIII MboI MboII MflI MluNI MnlI MscI MspA1I MspI MvaI MvnI MwoI NciI NdeII NlaIII NlaIV PleI PstI RsaI Sau3AI Sau96I ScrFI SfaNI SfcI SmlI TseI Tsp45I TspRI XhoII