(Circular) (Six-base) MAPSORT of: pFN21AE5608.seq Check: 1104 from: 1 to: 6130 pFN21AE5608 6130 bp With 182 enzymes: SgfI * December 8, 2014 13:40 .. AatII G_ACGT'C Cuts at: 278 331 414 600 1536 3940 278 Size: 53 83 186 936 2404 2468 Fragments arranged by size: 2468 2404 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 3473 1937 Size: 1536 4594 Acc65I G'GTAC_C Cuts at: 3451 3451 Size: 6130 AclI AA'CG_TT Cuts at: 1563 4257 4630 1563 Size: 2694 373 3063 Fragments arranged by size: 3063 2694 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 2360 4193 5577 5845 1632 Size: 728 1833 1384 268 1917 Fragments arranged by size: 1917 1833 1384 728 268 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 5933 AflIII A'CryG_T Cuts at: 1203 2453 2926 5029 6006 1203 Size: 1250 473 2103 977 1327 Fragments arranged by size: 2103 1327 1250 977 473 AgeI A'CCGG_T Cuts at: 4961 4961 Size: 6130 AhdI GACnn_n'nnGTC Cuts at: 4859 4859 Size: 6130 AleI CACnn'nnGTG Cuts at: 1166 1166 Size: 6130 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 1184 Size: 6130 AlwNI CAG_nnn'CTG Cuts at: 2021 3198 3422 3873 5445 2021 Size: 1177 224 451 1572 2706 Fragments arranged by size: 2706 1572 1177 451 224 ApaLI G'TGCA_C Cuts at: 4187 5343 4187 Size: 1156 4974 ApoI r'AATT_y Cuts at: 1493 3373 3439 3665 3817 5926 1493 Size: 1880 66 226 152 2109 1697 Fragments arranged by size: 2109 1880 1697 226 152 66 AseI AT'TA_AT Cuts at: 160 1032 4684 160 Size: 872 3652 1606 Fragments arranged by size: 3652 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 6130 AvaI C'yCGr_G Cuts at: 1870 1943 2559 2942 3455 1870 Size: 73 616 383 513 4545 Fragments arranged by size: 4545 616 513 383 73 BamHI G'GATC_C Cuts at: 3460 3827 3460 Size: 367 5763 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2334 2436 2573 2759 3451 Size: 325 217 627 547 102 137 186 692 Cuts at: 3451 4906 618 Size: 1455 1842 Fragments arranged by size: 1842 1455 692 627 547 325 217 186 137 102 BanII G_rGCy'C Cuts at: 1391 2981 3449 1391 Size: 1590 468 4072 Fragments arranged by size: 4072 1590 468 BbeI G_GCGC'C Cuts at: 2338 2338 Size: 6130 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 2953 928 Size: 971 1054 4105 Fragments arranged by size: 4105 1054 971 BbvCI CC'TCA_GC Cuts at: 2298 2410 2298 Size: 112 6018 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 4321 4355 1903 Size: 34 2384 34 3678 Fragments arranged by size: 3678 2384 34 34 BciVI GTATCCnnnnn_n' Cuts at: 4022 5232 4022 Size: 1210 4920 BclI T'GATC_A Cuts at: 1548 1548 Size: 6130 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 2009 2051 2976 Size: 107 122 71 631 605 337 42 925 Cuts at: 2976 4741 136 Size: 1765 1525 Fragments arranged by size: 1765 1525 925 631 605 337 122 107 71 42 BglII A'GATC_T Cuts at: 2148 6125 2148 Size: 3977 2153 BlpI GC'TnA_GC Cuts at: 3547 3547 Size: 6130 Bme1580I G_kGCm'C Cuts at: 1790 2439 4191 5347 1790 Size: 649 1752 1156 2573 Fragments arranged by size: 2573 1752 1156 649 BmgBI CAC'GTC Cuts at: 1318 2608 1318 Size: 1290 4840 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 3861 4819 442 Size: 416 53 83 399 24 2444 958 1753 Fragments arranged by size: 2444 1753 958 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 6130 BplI GAGnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 2167 2196 2167 Size: 29 6101 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2137 2237 3143 3277 4790 1239 Size: 898 100 906 134 1513 2579 Fragments arranged by size: 2579 1513 906 898 134 100 Bpu10I CC'TnA_GC Cuts at: 2298 2410 2298 Size: 112 6018 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 2375 2899 3269 4255 5120 5418 2375 Size: 524 370 986 865 298 3087 Fragments arranged by size: 3087 986 865 524 370 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 3062 4793 882 Size: 617 1563 1731 2219 Fragments arranged by size: 2219 1731 1563 617 BsaAI yAC'GTr Cuts at: 493 1204 2830 2927 3921 493 Size: 711 1626 97 994 2702 Fragments arranged by size: 2702 1626 994 711 97 BsaBI GATnn'nnATC Cuts at: 6124 6124 Size: 6130 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 2335 3937 4319 4954 Size: 53 83 186 936 802 1602 382 635 Cuts at: 4954 275 Size: 1451 Fragments arranged by size: 1602 1451 936 802 635 382 186 83 53 BsaWI w'CCGG_w Cuts at: 4563 4961 5235 5382 5793 4563 Size: 398 274 147 411 4900 Fragments arranged by size: 4900 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 2777 2807 2963 2993 1178 Size: 30 165 30 1374 30 156 30 4315 Fragments arranged by size: 4315 1374 165 156 30 30 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2159 2210 2213 2216 2219 2222 2225 2300 Size: 973 51 3 3 3 3 3 75 Cuts at: 2300 2303 2330 2617 2960 3023 3149 1186 Size: 3 27 287 343 63 126 4167 Fragments arranged by size: 4167 973 343 287 126 75 63 51 27 3 3 3 3 3 3 BseYI C'CCAG_C Cuts at: 2404 2903 2952 3317 3415 5333 5829 2404 Size: 499 49 365 98 1918 496 2705 Fragments arranged by size: 2705 1918 499 496 365 98 49 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 2211 2850 1163 Size: 573 475 639 4443 Fragments arranged by size: 4443 639 573 475 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 2791 3580 4341 5369 1540 Size: 275 186 790 789 761 1028 2301 Fragments arranged by size: 2301 1028 790 789 761 275 186 BsiHKAI G_wGCw'C Cuts at: 3015 3336 4191 4276 5347 3015 Size: 321 855 85 1071 3798 Fragments arranged by size: 3798 1071 855 321 85 BsmI GAATG_Cn' Cuts at: 2747 3641 3734 2747 Size: 894 93 5143 Fragments arranged by size: 5143 894 93 BsmBI CGTCTCn'nnnn_ Cuts at: 2828 2828 Size: 6130 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2439 2981 3015 3336 3449 4191 4276 Size: 399 649 542 34 321 113 742 85 Cuts at: 4276 5347 1391 Size: 1071 2174 Fragments arranged by size: 2174 1071 742 649 542 399 321 113 85 34 BspEI T'CCGG_A Cuts at: 5793 5793 Size: 6130 BspHI T'CATG_A Cuts at: 4019 5749 4019 Size: 1730 4400 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 3485 844 Size: 2641 3489 BsrBI CCG'CTC Cuts at: 1394 1823 4017 1394 Size: 429 2194 3507 Fragments arranged by size: 3507 2194 429 BsrDI GCAATG_nn' Cuts at: 65 1248 4625 4799 65 Size: 1183 3377 174 1396 Fragments arranged by size: 3377 1396 1183 174 BsrFI r'CCGG_y Cuts at: 1700 1810 2041 2072 4774 4961 1700 Size: 110 231 31 2702 187 2869 Fragments arranged by size: 2869 2702 231 187 110 31 BsrGI T'GTAC_A Cuts at: 96 2706 96 Size: 2610 3520 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 6130 BssSI C'ACGA_G Cuts at: 4190 5202 4190 Size: 1012 5118 BstAPI GCAn_nnn'nTGC Cuts at: 794 2873 794 Size: 2079 4051 BstBI TT'CG_AA Cuts at: 4934 4934 Size: 6130 BstEII G'GTnAC_C Cuts at: 1184 2654 1184 Size: 1470 4660 BstYI r'GATC_y Cuts at: 1972 2148 2394 2937 3460 3827 4212 4229 5681 Size: 176 246 543 523 367 385 17 1452 Cuts at: 5681 5767 5779 6125 1972 Size: 86 12 346 1977 Fragments arranged by size: 1977 1452 543 523 385 367 346 246 176 86 17 12 Bsu36I CC'TnA_GG Cuts at: 2193 2193 Size: 6130 BtgI C'CryG_G Cuts at: 513 1065 513 Size: 552 5578 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 487 Size: 683 56 5391 Fragments arranged by size: 5391 683 56 BtsI GCAGTG_nn' Cuts at: 2739 3221 3646 4440 4460 2739 Size: 482 425 794 20 4409 Fragments arranged by size: 4409 794 482 425 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 6130 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 6095 DraI TTT'AAA Cuts at: 3434 3434 Size: 6130 DraIII CAC_nnn'GTG Cuts at: 3019 3019 Size: 6130 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 5137 809 Size: 507 3821 1802 Fragments arranged by size: 3821 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2250 2593 2619 Size: 54 1425 50 275 18 420 343 26 Cuts at: 2619 2968 3177 3343 3577 4466 6095 8 Size: 349 209 166 234 889 1629 43 Fragments arranged by size: 1629 1425 889 420 349 343 275 234 209 166 54 50 43 26 18 EagI C'GGCC_G Cuts at: 1537 1812 3577 1537 Size: 275 1765 4090 Fragments arranged by size: 4090 1765 275 EarI CTCTTCn'nnn_ Cuts at: 1353 2846 4060 1353 Size: 1493 1214 3423 Fragments arranged by size: 3423 1493 1214 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 4717 5091 5237 387 Size: 1064 138 3128 374 146 1280 Fragments arranged by size: 3128 1280 1064 374 146 138 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2137 2237 2360 3143 3277 4193 4790 Size: 393 505 100 123 783 134 916 597 Cuts at: 4790 5577 5845 1239 Size: 787 268 1524 Fragments arranged by size: 1524 916 787 783 597 505 393 268 134 123 100 EcoNI CCTnn'n_nnAGG Cuts at: 2342 2342 Size: 6130 EcoO109I rG'GnC_Cy Cuts at: 2401 3403 2401 Size: 1002 5128 EcoRI G'AATT_C Cuts at: 3373 3439 3817 3373 Size: 66 378 5686 Fragments arranged by size: 5686 378 66 EcoRV GAT'ATC Cuts at: 3141 3141 Size: 6130 FalI AAGnnnnnCTTnnnnnnnn_nnnnn' Cuts at: 2263 2263 Size: 6130 FspI TGC'GCA Cuts at: 4636 4636 Size: 6130 HaeII r_GCGC'y Cuts at: 1678 2338 5277 1678 Size: 660 2939 2531 Fragments arranged by size: 2939 2531 660 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2720 2752 3023 3055 4771 Size: 32 135 32 1148 32 271 32 1716 Cuts at: 4771 4803 4845 4877 1373 Size: 32 42 32 2626 Fragments arranged by size: 2626 1716 1148 271 135 42 32 32 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 3474 3720 669 Size: 988 281 1536 246 3079 Fragments arranged by size: 3079 1536 988 281 246 HindIII A'AGCT_T Cuts at: 748 748 Size: 6130 HpaI GTT'AAC Cuts at: 3720 3720 Size: 6130 KasI G'GCGC_C Cuts at: 2334 2334 Size: 6130 KpnI G_GTAC'C Cuts at: 3455 3455 Size: 6130 MfeI C'AATT_G Cuts at: 3729 3729 Size: 6130 MluI A'CGCG_T Cuts at: 6006 6006 Size: 6130 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 2655 5244 5428 1305 Size: 1350 2589 184 2007 Fragments arranged by size: 2589 2007 1350 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 2252 2595 2621 2970 3179 Size: 54 1425 343 420 343 26 349 209 Cuts at: 3179 10 Size: 2961 Fragments arranged by size: 2961 1425 420 349 343 343 209 54 26 MslI CAynn'nnrTG Cuts at: 518 1166 2727 4088 4447 4606 518 Size: 648 1561 1361 359 159 2042 Fragments arranged by size: 2042 1561 1361 648 359 159 MspA1I CmG'CkG Cuts at: 1606 2018 2414 3419 3546 4223 5371 5616 1606 Size: 412 396 1005 127 677 1148 245 2120 Fragments arranged by size: 2120 1148 1005 677 412 396 245 127 NaeI GCC'GGC Cuts at: 2043 2074 2043 Size: 31 6099 NarI GG'CG_CC Cuts at: 2335 2335 Size: 6130 NcoI C'CATG_G Cuts at: 513 1065 513 Size: 552 5578 NdeI CA'TA_TG Cuts at: 387 387 Size: 6130 NgoMIV G'CCGG_C Cuts at: 2041 2072 2041 Size: 31 6099 NheI G'CTAG_C Cuts at: 1052 1052 Size: 6130 NotI GC'GGCC_GC Cuts at: 3577 3577 Size: 6130 NspI r_CATG'y Cuts at: 1132 1229 2457 2552 3488 5033 1132 Size: 97 1228 95 936 1545 2229 Fragments arranged by size: 2229 1545 1228 936 97 95 PasI CC'CwG_GG Cuts at: 1348 1348 Size: 6130 PciI A'CATG_T Cuts at: 2453 5029 2453 Size: 2576 3554 PflMI CCAn_nnn'nTGG Cuts at: 1108 3422 1108 Size: 2314 3816 PmeI GTTT'AAAC Cuts at: 3434 3434 Size: 6130 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 4199 4231 5712 5744 4199 Size: 32 1481 32 4585 Fragments arranged by size: 4585 1481 32 32 PpuMI rG'GwC_Cy Cuts at: 3403 3403 Size: 6130 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 6130 PsiI TTA'TAA Cuts at: 3700 3700 Size: 6130 PspXI vC'TCGA_Gb Cuts at: 2559 2559 Size: 6130 PstI C_TGCA'G Cuts at: 830 2376 2510 3087 3482 830 Size: 1546 134 577 395 3478 Fragments arranged by size: 3478 1546 577 395 134 PvuI CG_AT'CG Cuts at: 2001 2791 2001 Size: 790 5340 PvuII CAG'CTG Cuts at: 2414 3419 2414 Size: 1005 5125 SalI G'TCGA_C Cuts at: 1936 3472 1936 Size: 1536 4594 SanDI GG'GwC_CC Cuts at: 3403 3403 Size: 6130 SapI GCTCTTCn'nnn_ Cuts at: 2846 2846 Size: 6130 SbfI CC_TGCA'GG Cuts at: 3482 3482 Size: 6130 ScaI AGT'ACT Cuts at: 4378 6090 4378 Size: 1712 4418 SexAI A'CCwGG_T Cuts at: 2322 2322 Size: 6130 SfcI C'TryA_G Cuts at: 826 1045 2372 2506 2773 3083 3478 4613 5294 Size: 219 1327 134 267 310 395 1135 681 Cuts at: 5294 5485 826 Size: 191 1471 Fragments arranged by size: 1471 1327 1135 681 395 310 267 219 191 134 SfiI GGCCn_nnn'nGGCC Cuts at: 2051 2051 Size: 6130 SfoI GGC'GCC Cuts at: 2336 2336 Size: 6130 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 6130 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 6130 SmaI CCC'GGG Cuts at: 1872 3457 1872 Size: 1585 4545 SmlI C'TyrA_G Cuts at: 820 1017 1943 2354 2559 2878 3284 4234 5135 Size: 197 926 411 205 319 406 950 901 Cuts at: 5135 5397 820 Size: 262 1553 Fragments arranged by size: 1553 950 926 901 411 406 319 262 205 197 SnaBI TAC'GTA Cuts at: 493 3921 493 Size: 3428 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 6130 SphI G_CATG'C Cuts at: 1132 3488 1132 Size: 2356 3774 SspI AAT'ATT Cuts at: 5 52 4054 5 Size: 47 4002 2081 Fragments arranged by size: 4002 2081 47 StuI AGG'CCT Cuts at: 1517 3120 1517 Size: 1603 4527 StyI C'CwwG_G Cuts at: 513 1065 2121 2183 3569 513 Size: 552 1056 62 1386 3074 Fragments arranged by size: 3074 1386 1056 552 62 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 2777 4157 4174 4327 4512 4957 325 Size: 1255 1197 1380 17 153 185 445 1498 Fragments arranged by size: 1498 1380 1255 1197 445 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 2706 4376 6088 Size: 274 80 33 51 1443 729 1670 1712 Cuts at: 6088 96 Size: 138 Fragments arranged by size: 1712 1670 1443 729 274 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 2777 2809 2911 2943 2963 2995 3005 3037 6090 Size: 32 102 32 20 32 10 32 3053 Cuts at: 6090 6122 2777 Size: 32 2785 Fragments arranged by size: 3053 2785 102 32 32 32 32 32 20 10 Tth111I GACn'n_nGTC Cuts at: 1316 1316 Size: 6130 XbaI T'CTAG_A Cuts at: 3466 3466 Size: 6130 XhoI C'TCGA_G Cuts at: 1943 2559 1943 Size: 616 5514 XmaI C'CCGG_G Cuts at: 1870 3455 1870 Size: 1585 4545 XmnI GAAnn'nnTTC Cuts at: 4259 4259 Size: 6130 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 3938 276 Size: 53 83 186 936 2404 2468 Fragments arranged by size: 2468 2404 936 186 83 53 Enzymes that do cut: AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbeI BbsI BbvCI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BplI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI BsmBI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstYI Bsu36I BtgI BtgZI BtsI ClaI CspCI DraI DraIII DrdI EaeI EagI EarI EciI Eco57MI EcoNI EcoO109I EcoRI EcoRV FalI FspI HaeII Hin4I HincII HindIII HpaI KasI KpnI MfeI MluI MmeI MscI MslI MspA1I NaeI NarI NcoI NdeI NgoMIV NheI NotI NspI PasI PciI PflMI PmeI PpiI PpuMI PshAI PsiI PspXI PstI PvuI PvuII SalI SanDI SapI SbfI ScaI SexAI SfcI SfiI SfoI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XhoI XmaI XmnI ZraI Enzymes that do not cut: AarI AfeI AloI ApaI AscI AvrII BaeI BfrBI BsiWI BstXI BstZ17I EcoICRI FseI FspAI NruI NsiI PacI PfoI PmlI PspOMI PsrI RsrII SacI SacII SrfI SwaI XcmI