Restriction map for
pFN21AE5608
(Linear) (Six-base) MAPSORT of: KIEE5608.seq Check: 7947 from: 1 to: 1425
KIEE5608.orf
With 182 enzymes: SgfI *
December 5, 2014 13:02 ..
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 355 1425
Size: 355 1070
AflIII A'CryG_T
Cuts at: 0 448 921 1425
Size: 448 473 504
Fragments arranged by size:
504 473 448
AlwNI CAG_nnn'CTG
Cuts at: 0 16 1193 1417 1425
Size: 16 1177 224 8
Fragments arranged by size:
1177 224 16 8
ApoI r'AATT_y
Cuts at: 0 1368 1425
Size: 1368 57
AvaI C'yCGr_G
Cuts at: 0 554 937 1425
Size: 554 383 488
Fragments arranged by size:
554 488 383
BanI G'GyrC_C
Cuts at: 0 329 431 568 754 1425
Size: 329 102 137 186 671
Fragments arranged by size:
671 329 186 137 102
BanII G_rGCy'C
Cuts at: 0 976 1425
Size: 976 449
BbeI G_GCGC'C
Cuts at: 0 333 1425
Size: 333 1092
BbsI GAAGACnn'nnnn_
Cuts at: 0 948 1425
Size: 948 477
BbvCI CC'TCA_GC
Cuts at: 0 293 405 1425
Size: 293 112 1020
Fragments arranged by size:
1020 293 112
BglI GCCn_nnn'nGGC
Cuts at: 0 46 971 1425
Size: 46 925 454
Fragments arranged by size:
925 454 46
BglII A'GATC_T
Cuts at: 0 143 1425
Size: 143 1282
Bme1580I G_kGCm'C
Cuts at: 0 434 1425
Size: 434 991
BmgBI CAC'GTC
Cuts at: 0 603 1425
Size: 603 822
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 162 191 1425
Size: 162 29 1234
Fragments arranged by size:
1234 162 29
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 132 232 1138 1272 1425
Size: 132 100 906 134 153
Fragments arranged by size:
906 153 134 132 100
Bpu10I CC'TnA_GC
Cuts at: 0 293 405 1425
Size: 293 112 1020
Fragments arranged by size:
1020 293 112
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 370 894 1264 1425
Size: 370 524 370 161
Fragments arranged by size:
524 370 370 161
BsaI GGTCTCn'nnnn_
Cuts at: 0 1057 1425
Size: 1057 368
BsaAI yAC'GTr
Cuts at: 0 825 922 1425
Size: 825 97 503
Fragments arranged by size:
825 503 97
BsaHI Gr'CG_yC
Cuts at: 0 330 1425
Size: 330 1095
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 772 802 958 988 1425
Size: 772 30 156 30 437
Fragments arranged by size:
772 437 156 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 154 205 208 211 214 217 220 295
Size: 154 51 3 3 3 3 3 75
Cuts at: 295 298 325 612 955 1018 1144 1425
Size: 3 27 287 343 63 126 281
Fragments arranged by size:
343 287 281 154 126 75 63 51
27 3 3 3 3 3 3
BseYI C'CCAG_C
Cuts at: 0 399 898 947 1312 1410 1425
Size: 399 499 49 365 98 15
Fragments arranged by size:
499 399 365 98 49 15
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 206 845 1425
Size: 206 639 580
Fragments arranged by size:
639 580 206
BsiEI CG_ry'CG
Cuts at: 0 786 1425
Size: 786 639
BsiHKAI G_wGCw'C
Cuts at: 0 1010 1331 1425
Size: 1010 321 94
Fragments arranged by size:
1010 321 94
BsmI GAATG_Cn'
Cuts at: 0 742 1425
Size: 742 683
BsmBI CGTCTCn'nnnn_
Cuts at: 0 823 1425
Size: 823 602
Bsp1286I G_dGCh'C
Cuts at: 0 434 976 1010 1331 1425
Size: 434 542 34 321 94
Fragments arranged by size:
542 434 321 94 34
BsrFI r'CCGG_y
Cuts at: 0 36 67 1425
Size: 36 31 1358
Fragments arranged by size:
1358 36 31
BsrGI T'GTAC_A
Cuts at: 0 701 1425
Size: 701 724
BstAPI GCAn_nnn'nTGC
Cuts at: 0 868 1425
Size: 868 557
BstEII G'GTnAC_C
Cuts at: 0 649 1425
Size: 649 776
BstYI r'GATC_y
Cuts at: 0 143 389 932 1425
Size: 143 246 543 493
Fragments arranged by size:
543 493 246 143
Bsu36I CC'TnA_GG
Cuts at: 0 188 1425
Size: 188 1237
BtsI GCAGTG_nn'
Cuts at: 0 734 1216 1425
Size: 734 482 209
Fragments arranged by size:
734 482 209
DraIII CAC_nnn'GTG
Cuts at: 0 1014 1425
Size: 1014 411
EaeI y'GGCC_r
Cuts at: 0 245 588 614 963 1172 1338 1425
Size: 245 343 26 349 209 166 87
Fragments arranged by size:
349 343 245 209 166 87 26
EarI CTCTTCn'nnn_
Cuts at: 0 841 1425
Size: 841 584
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 132 232 355 1138 1272 1425
Size: 132 100 123 783 134 153
Fragments arranged by size:
783 153 134 132 123 100
EcoNI CCTnn'n_nnAGG
Cuts at: 0 337 1425
Size: 337 1088
EcoO109I rG'GnC_Cy
Cuts at: 0 396 1398 1425
Size: 396 1002 27
Fragments arranged by size:
1002 396 27
EcoRI G'AATT_C
Cuts at: 0 1368 1425
Size: 1368 57
EcoRV GAT'ATC
Cuts at: 0 1136 1425
Size: 1136 289
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 0 258 1425
Size: 258 1167
HaeII r_GCGC'y
Cuts at: 0 333 1425
Size: 333 1092
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 715 747 1018 1050 1425
Size: 715 32 271 32 375
Fragments arranged by size:
715 375 271 32 32
KasI G'GCGC_C
Cuts at: 0 329 1425
Size: 329 1096
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 650 1425
Size: 650 775
MscI TGG'CCA
Cuts at: 0 247 590 616 965 1174 1425
Size: 247 343 26 349 209 251
Fragments arranged by size:
349 343 251 247 209 26
MslI CAynn'nnrTG
Cuts at: 0 722 1425
Size: 722 703
MspA1I CmG'CkG
Cuts at: 0 13 409 1414 1425
Size: 13 396 1005 11
Fragments arranged by size:
1005 396 13 11
NaeI GCC'GGC
Cuts at: 0 38 69 1425
Size: 38 31 1356
Fragments arranged by size:
1356 38 31
NarI GG'CG_CC
Cuts at: 0 330 1425
Size: 330 1095
NgoMIV G'CCGG_C
Cuts at: 0 36 67 1425
Size: 36 31 1358
Fragments arranged by size:
1358 36 31
NspI r_CATG'y
Cuts at: 0 452 547 1425
Size: 452 95 878
Fragments arranged by size:
878 452 95
PciI A'CATG_T
Cuts at: 0 448 1425
Size: 448 977
PflMI CCAn_nnn'nTGG
Cuts at: 0 1417 1425
Size: 1417 8
PpuMI rG'GwC_Cy
Cuts at: 0 1398 1425
Size: 1398 27
PspXI vC'TCGA_Gb
Cuts at: 0 554 1425
Size: 554 871
PstI C_TGCA'G
Cuts at: 0 371 505 1082 1425
Size: 371 134 577 343
Fragments arranged by size:
577 371 343 134
PvuI CG_AT'CG
Cuts at: 0 786 1425
Size: 786 639
PvuII CAG'CTG
Cuts at: 0 409 1414 1425
Size: 409 1005 11
Fragments arranged by size:
1005 409 11
SanDI GG'GwC_CC
Cuts at: 0 1398 1425
Size: 1398 27
SapI GCTCTTCn'nnn_
Cuts at: 0 841 1425
Size: 841 584
SexAI A'CCwGG_T
Cuts at: 0 317 1425
Size: 317 1108
SfcI C'TryA_G
Cuts at: 0 367 501 768 1078 1425
Size: 367 134 267 310 347
Fragments arranged by size:
367 347 310 267 134
SfiI GGCCn_nnn'nGGCC
Cuts at: 0 46 1425
Size: 46 1379
SfoI GGC'GCC
Cuts at: 0 331 1425
Size: 331 1094
SmlI C'TyrA_G
Cuts at: 0 349 554 873 1279 1425
Size: 349 205 319 406 146
Fragments arranged by size:
406 349 319 205 146
StuI AGG'CCT
Cuts at: 0 1115 1425
Size: 1115 310
StyI C'CwwG_G
Cuts at: 0 116 178 1425
Size: 116 62 1247
Fragments arranged by size:
1247 116 62
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 772 1425
Size: 772 653
TatI w'GTAC_w
Cuts at: 0 701 1425
Size: 701 724
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 772 804 906 938 958 990 1000 1032
Size: 772 32 102 32 20 32 10 32
Cuts at: 1032 1425
Size: 393
Fragments arranged by size:
772 393 102 32 32 32 32 20
10
XhoI C'TCGA_G
Cuts at: 0 554 1425
Size: 554 871
Enzymes that do cut:
AcuI AflIII AlwNI ApoI AvaI BanI BanII BbeI
BbsI BbvCI BglI BglII Bme1580I BmgBI BplI BpmI
Bpu10I BpuEI BsaI BsaAI BsaHI BsaXI BseRI BseYI
BsgI BsiEI BsiHKAI BsmI BsmBI Bsp1286I BsrFI BsrGI
BstAPI BstEII BstYI Bsu36I BtsI DraIII EaeI EarI
Eco57MI EcoNI EcoO109I EcoRI EcoRV FalI HaeII Hin4I
KasI MmeI MscI MslI MspA1I NaeI NarI NgoMIV
NspI PciI PflMI PpuMI PspXI PstI PvuI PvuII
SanDI SapI SexAI SfcI SfiI SfoI SmlI StuI
StyI TaqII TatI TstI XhoI
Enzymes that do not cut:
AarI AatII AccI Acc65I AclI AfeI AflII AgeI
AhdI AleI AlfI AloI ApaI ApaLI AscI AseI
AsiSI AvrII BaeI BamHI BcgI BciVI BclI BfrBI
BlpI BmrI BmtI BsaBI BsaWI BsiWI BspEI BspHI
BspMI BsrBI BsrDI BssHII BssSI BstBI BstXI BstZ17I
BtgI BtgZI ClaI CspCI DraI DrdI EagI EciI
EcoICRI FseI FspI FspAI HincII HindIII HpaI KpnI
MfeI MluI NcoI NdeI NheI NotI NruI NsiI
PacI PasI PfoI PmeI PmlI PpiI PshAI PsiI
PspOMI PsrI RsrII SacI SacII SalI SbfI ScaI
SgfI SgrAI SmaI SnaBI SpeI SphI SrfI SspI
SwaI Tth111I XbaI XcmI XmaI XmnI ZraI