################################## # NO CUT ENZYMES ( 189 enzymes) # ################################## AatII Acc65I AccII AccIII AclI AcsI AcyI AfaI AflII AflIII AgeI AhdI AlwNI AocI Aor51HI ApoI AscI AseI AsnI Asp700I Asp718I AspEI AspI AvaI AvrII BalI BamHI BanI BbeI BbrPI BbsI BcgI BciVI BclI BcnI BfaI BfrI BglI BglII BlnI BlpI BpmI Bpu1102I BpuAI BsaAI BsaBI BsaHI BsaI BsaWI BseAI BsgI BsiWI BsmBI BsmFI BsmI BsoBI Bsp1407I BspDI BspEI BspHI BspLU11I BspMI BsrBI BsrDI BsrFI BsrGI BssHII Bst1107I BstAPI BstBI BstEII BstPI BstUI BstXI BstYI BstZ17I Bsu36I CelII Cfr10I ClaI CpoI DraI DraIII DrdI DsaI EaeI EagI Eam1105I EarI EclXI Eco47III Eco52I Eco81I EcoNI EcoO65I EcoRI EcoRV EcoT14I EcoT22I FauI FbaI FseI HaeII HapII Hin1I HincII HindII HindIII HinfI HpaI HpaII KasI KpnI Ksp632I KspI MaeI MaeIII MamI MfeI MflI MluI MluNI MroI MscI MspA1I MspI MunI MvnI NaeI NarI NciI NcoI NdeI NgoMIV NheI NotI NruI NsiI NspI NspV PacI PaeR7I PflFI PflMI PinAI PleI PmaCI PmeI PmlI PpuMI PshAI PshBI Psp1406I PvuII RcaI RsaI RsrII SacI SacII SalI SapI SbfI ScaI SexAI SfiI SfoI SfuI SgrAI SmaI SmlI SnaBI SpeI SphI Sse8387I SspBI SspI StuI StyI SwaI TfiI Tsp45I Tth111I Van91I XbaI XhoI XhoII XmaCI XmaI XmnI ################################### # ONE CUT ENZYMES ( 41 enzymes) # ################################### AccI 400 GTCTAC GT^[AC][GT]AC AciI 376 GCGG CCGC(-3/-1) AluI 296 AGCT AG^CT Alw44I 390 GTGCAC G^TGCAC AlwI 360 GATCC GGATC(4/5) ApaI 312 GGGCCC GGGCC^C ApaLI 390 GTGCAC G^TGCAC AspHI 390 GTGCAC G[AT]GC[AT]^C AvaII 329 GGACC G^G[AT]CC AviII 176 TGCGCA TGC^GCA BanII 312 GGGCCC G[GA]GC[CT]^C BbvCI 84 GCTGAGG CCTCAGC(-5/-2) BsaJI 395 CCAGGG C^C[ACGT][ACGT]GG BsiEI 34 CGATCG CG[GA][CT]^CG BsiHKAI 390 GTGCAC G[AT]GC[AT]^C BsiYI 344 CCAACGATGGG CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG BslI 344 CCAACGATGGG CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG BsmAI 143 GAGAC GTCTC(1/5) BsrI 20 ACTGG ACTGG(1/-1) BssSI 324 CTCGTG CACGAG(-5/-1) Cac8I 117 GCCAGC GC[ACGT]^[ACGT]GC CfoI 177 GCGC GCG^C DdeI 85 CTGAG C^T[ACGT]AG FspI 176 TGCGCA TGC^GCA HgaI 109 GCGTC GACGC(5/10) HhaI 177 GCGC GCG^C HinP1I 177 GCGC G^CGC MboII 90 GAAGA GAAGA(8/7) MseI 191 TTAA T^TAA MslI 168 CACCACCGTG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG NlaIII 369 CATG CATG^ PstI 104 CTGCAG CTGCA^G PvuI 34 CGATCG CGAT^CG SfaNI 81 GATGC GCATC(5/9) SfcI 104 CTGCAG C^T[GA][CT]AG TaqI 47 TCGA T^CGA Tru9I 191 TTAA T^TAA Tsp509I 53 AATT ^AATT TspRI 122 CAGTG CA[GC]TG[ACGT][ACGT]^ TthHB8I 47 TCGA T^CGA XcmI 113 CCAAGCCAGCAGTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG ################################### # TWO CUT ENZYMES ( 17 enzymes) # ################################### BbvI 106 GCAGC GCAGC(8/12) 103 GCTGC BmyI 312 GGGCCC G[AGT]GC[ACT]^C 390 GTGCAC BseRI 208 GAGGAG GAGGAG(10/8) 217 GAGGAG Bsp1286I 312 GGGCCC G[AGT]GC[ACT]^C 390 GTGCAC BssKI 252 CCTGG ^CC[ACGT]GG 395 CCAGG BstNI 252 CCTGG CC^[AT]GG 395 CCAGG EcoRII 252 CCTGG ^CC[AT]GG 395 CCAGG Fnu4HI 103 GCTGC GC^[ACGT]GC 106 GCAGC HaeIII 239 GGCC GG^CC 313 GGCC HphI 72 GGTGA GGTGA(8/7) 255 GGTGA ItaI 103 GCTGC GC^[ACGT]GC 106 GCAGC MaeII 383 ACGT A^CGT 404 ACGT MvaI 252 CCTGG CC^[AT]GG 395 CCAGG MwoI 57 GCTCAAAGTGC GC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GC 97 GCTGTGGCTGC NlaIV 312 GGGCCC GG[ACGT]^[ACGT]CC 313 GGCCCC ScrFI 252 CCTGG CC^[ACGT]GG 395 CCAGG TseI 103 GCTGC G^C[AT]GC 106 GCAGC ######################################### # 3 OR MORE CUT ENZYMES ( 11 enzymes) # ######################################### BstF5I Cfr13I DpnII DraII EcoO109I FokI MboI MnlI NdeII Sau3AI Sau96I