################################## # NO CUT ENZYMES ( 128 enzymes) # ################################## AatII Acc65I AccI AccIII AclI AcsI AflII AflIII AgeI AhdI Alw44I AlwNI Aor51HI ApaLI ApoI AscI AseI AsnI Asp700I Asp718I AspEI AspI AviII AvrII BamHI BbeI BbrPI BcgI BclI BfrI BglII BlnI BlpI Bpu1102I BsaAI BsaBI BsaWI BseAI BseRI BsiEI BsiWI BsmBI BsmI Bsp1407I BspDI BspEI BspHI BspLU11I BsrDI BsrGI BssSI Bst1107I BstZ17I CelII ClaI CpoI DraI DrdI EagI Eam1105I EarI EclXI Eco47III Eco52I EcoRI EcoRV EcoT22I FbaI FseI FspI HincII HindII HindIII HpaI KasI KpnI Ksp632I KspI MamI MfeI MluI MroI MseI MunI NarI NdeI NheI NotI NruI NsiI NspI PacI PaeR7I PflFI PinAI PmaCI PmeI PmlI PshAI PshBI Psp1406I PvuI RcaI RsrII SacII SalI SapI SbfI SexAI SfiI SfoI SgrAI SmlI SnaBI SpeI SphI Sse8387I SspBI SspI StuI SwaI TfiI Tru9I Tsp509I Tth111I XbaI XhoI XmnI ################################### # ONE CUT ENZYMES ( 42 enzymes) # ################################### AcyI 315 GACGCC G[GA]^CG[CT]C AocI 1398 CCTGAGG CC^T[ACGT]AGG ApaI 950 GGGCCC GGGCC^C BbsI 486 GAAGAC GAAGAC(2/6) BbvCI 742 GCTGAGG CCTCAGC(-5/-2) BciVI 521 GTATCC GTATCC(6/5) BpuAI 486 GAAGAC GAAGAC(2/6) BsaHI 315 GACGCC G[GA]^CG[CT]C BsmFI 1091 GTCCC GGGAC(10/14) BsrBI 1444 GAGCGG CCGCTC(-3/-3) BsrFI 154 GCCGGC [GA]^CCGG[CT] BssHII 76 GCGCGC G^CGCGC BstBI 782 TTCGAA TT^CGAA BstEII 226 GGTGACC G^GT[ACGT]ACC BstPI 226 GGTGACC G^GT[ACGT]ACC BstXI 1103 CCAATGTCCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG BstYI 386 AGATCC [GA]^GATC[CT] Bsu36I 1398 CCTGAGG CC^T[ACGT]AGG Cfr10I 154 GCCGGC [GA]^CCGG[CT] Eco81I 1398 CCTGAGG CC^T[ACGT]AGG EcoNI 282 CCTGAGCAAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG EcoO65I 226 GGTGACC G^GT[ACGT]ACC HgaI 315 GACGC GACGC(5/10) Hin1I 315 GACGCC G[GA]^CG[CT]C MaeII 855 ACGT A^CGT MboII 486 GAAGA GAAGA(8/7) MflI 386 AGATCC [GA]^GATC[CT] MspA1I 43 CAGCTG C[AC]G^C[GT]G NaeI 154 GCCGGC GCC^GGC NgoMIV 154 GCCGGC G^CCGGC NspV 782 TTCGAA TT^CGAA PflMI 1211 CCATCCTCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG PpuMI 1334 GGGTCCT [GA]G^G[AT]CC[CT] PvuII 43 CAGCTG CAG^CTG SacI 1237 GAGCTC GAGCT^C ScaI 447 AGTACT AGT^ACT SfuI 782 TTCGAA TT^CGAA SmaI 1331 CCCGGG CCC^GGG Van91I 1211 CCATCCTCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG XhoII 386 AGATCC [GA]^GATC[CT] XmaCI 1331 CCCGGG C^CCGGG XmaI 1331 CCCGGG C^CCGGG ################################### # TWO CUT ENZYMES ( 16 enzymes) # ################################### AvaI 806 CCCGAG C^[CT]CG[GA]G 1331 CCCGGG AvaII 1335 GGTCC G^G[AT]CC 1425 GGACC BanI 730 GGCACC G^G[CT][GA]CC 1126 GGCACC BglI 1300 GCCAAGGTGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC 1366 GCCACAGTGGC BpmI 477 CTCCAG CTGGAG(16/14) 1019 CTGGAG BsaI 1393 GAGACC GGTCTC(1/5) 1220 GGTCTC BsmAI 1393 GAGAC GTCTC(1/5) 1221 GTCTC BsoBI 806 CCCGAG C^[CT]CG[GA]G 1331 CCCGGG BspMI 26 ACCTGC ACCTGC(4/8) 490 ACCTGC DraIII 898 CACCATGTG CAC[ACGT][ACGT][ACGT]^GTG 911 CACTGAGTG HaeII 151 AGCGCC [GA]GCGC^[CT] 1409 GGCGCT HinfI 162 GACTC G^A[ACGT]TC 1341 GAGTC NcoI 1251 CCATGG C^CATGG 1295 CCATGG PleI 162 GACTC GAGTC(4/5) 1341 GAGTC PstI 649 CTGCAG CTGCA^G 1042 CTGCAG XcmI 927 CCAGGAAGTGTGTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG 1295 CCATGGCCAAGGTGG ######################################### # 3 OR MORE CUT ENZYMES ( 72 enzymes) # ######################################### AccII AciI AfaI AluI AlwI AspHI BalI BanII BbvI BcnI BfaI BmyI BsaJI BsgI BsiHKAI BsiYI BslI Bsp1286I BsrI BssKI BstAPI BstF5I BstNI BstUI Cac8I CfoI Cfr13I DdeI DpnII DraII DsaI EaeI EcoO109I EcoRII EcoT14I FauI Fnu4HI FokI HaeIII HapII HhaI HinP1I HpaII HphI ItaI MaeI MaeIII MboI MluNI MnlI MscI MslI MspI MvaI MvnI MwoI NciI NdeII NlaIII NlaIV RsaI Sau3AI Sau96I ScrFI SfaNI SfcI StyI TaqI TseI Tsp45I TspRI TthHB8I