Restriction map for
pFN21AB0980
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# NO CUT ENZYMES ( 127 enzymes) #
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AatII Acc65I AclI AcsI AflII
AflIII AgeI AhdI AlwNI AocI
Aor51HI ApoI AscI Asp700I Asp718I
AspEI AspI AviII AvrII BamHI
BanI BbeI BbrPI BbsI BcgI
BfrI BlnI BpuAI BsaAI BsaBI
BsiEI BsiWI BsmBI BsmI Bsp1407I
BspDI BspHI BspLU11I BsrGI BssHII
BssSI Bst1107I BstAPI BstBI BstEII
BstPI BstZ17I Bsu36I ClaI CpoI
DraI DraIII DrdI EagI Eam1105I
EarI EclXI Eco47III Eco52I Eco81I
EcoO65I EcoRI EcoRV EcoT22I FseI
FspI HincII HindII HindIII HpaI
KasI KpnI Ksp632I KspI MaeII
MamI MfeI MluI MunI NaeI
NarI NdeI NgoMIV NheI NotI
NruI NsiI NspI NspV PacI
PflFI PflMI PinAI PmaCI PmeI
PmlI PshAI Psp1406I PvuI RcaI
RsrII SacI SacII SalI SapI
SbfI ScaI SexAI SfiI SfoI
SfuI SgrAI SmaI SnaBI SpeI
SphI Sse8387I SspBI SspI SwaI
Tsp509I Tth111I Van91I XbaI XmaCI
XmaI XmnI
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# ONE CUT ENZYMES ( 41 enzymes) #
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AccI 103 GTAGAC GT^[AC][GT]AC
AccII 154 CGCG CG^CG
AccIII 395 TCCGGA T^CCGGA
AcyI 343 GGCGTC G[GA]^CG[CT]C
Alw44I 1313 GTGCAC G^TGCAC
ApaI 1102 GGGCCC GGGCC^C
ApaLI 1313 GTGCAC G^TGCAC
AseI 382 ATTAAT AT^TAAT
AsnI 382 ATTAAT AT^TAAT
AspHI 1313 GTGCAC G[AT]GC[AT]^C
BbvCI 337 GCTGAGG CCTCAGC(-5/-2)
BciVI 1117 GGATAC GTATCC(6/5)
BclI 603 TGATCA T^GATCA
BglI 849 GCCACTGGGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
BglII 19 AGATCT A^GATCT
BsaHI 343 GGCGTC G[GA]^CG[CT]C
BsaI 323 GAGACC GGTCTC(1/5)
BsaWI 395 TCCGGA [AT]^CCGG[AT]
BseAI 395 TCCGGA T^CCGGA
BsgI 406 GTGCAG GTGCAG(16/14)
BsiHKAI 1313 GTGCAC G[AT]GC[AT]^C
BspEI 395 TCCGGA T^CCGGA
BspMI 188 GCAGGT ACCTGC(4/8)
BsrDI 1205 GCAATG GCAATG(2/0)
BsrFI 326 ACCGGC [GA]^CCGG[CT]
BstUI 154 CGCG CG^CG
BstXI 257 CCAAGGTGGTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
Cfr10I 326 ACCGGC [GA]^CCGG[CT]
EcoNI 333 CCTGGCTGAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
FbaI 603 TGATCA T^GATCA
HaeII 1250 GGCGCT [GA]GCGC^[CT]
Hin1I 343 GGCGTC G[GA]^CG[CT]C
MboII 270 GAAGA GAAGA(8/7)
MroI 395 TCCGGA T^CCGGA
MseI 383 TTAA T^TAA
MslI 115 CACCAGGGTG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
MvnI 154 CGCG CG^CG
PpuMI 1013 GGGTCCC [GA]G^G[AT]CC[CT]
PshBI 382 ATTAAT AT^TAAT
PstI 994 CTGCAG CTGCA^G
Tru9I 383 TTAA T^TAA
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# TWO CUT ENZYMES ( 19 enzymes) #
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AfaI 192 GTAC GT^AC
1053 GTAC
BfaI 950 CTAG C^TAG
974 CTAG
BlpI 485 GCTCAGC GC^T[ACGT]AGC
565 GCTCAGC
BpmI 1263 CTCCAG CTGGAG(16/14)
218 CTGGAG
Bpu1102I 485 GCTCAGC GC^T[ACGT]AGC
565 GCTCAGC
BsmAI 199 GAGAC GTCTC(1/5)
323 GAGAC
BsrBI 703 CCGCTC CCGCTC(-3/-3)
721 GAGCGG
CelII 485 GCTCAGC GC^T[ACGT]AGC
565 GCTCAGC
FauI 786 CCCGC CCCGC(4/6)
1017 CCCGC
MaeI 950 CTAG C^TAG
974 CTAG
NcoI 27 CCATGG C^CATGG
162 CCATGG
PaeR7I 697 CTCGAG C^TCGAG
706 CTCGAG
RsaI 192 GTAC GT^AC
1053 GTAC
SfcI 994 CTGCAG C^T[GA][CT]AG
1236 CTACAG
SmlI 697 CTCGAG C^T[CT][GA]AG
706 CTCGAG
StuI 740 AGGCCT AGG^CCT
1244 AGGCCT
TfiI 389 GAATC G^A[AT]TC
520 GATTC
Tsp45I 350 GTGAC ^GT[GC]AC
507 GTCAC
XhoI 697 CTCGAG C^TCGAG
706 CTCGAG
#########################################
# 3 OR MORE CUT ENZYMES ( 71 enzymes) #
#########################################
AciI AluI AlwI AvaI AvaII
BalI BanII BbvI BcnI BmyI
BsaJI BseRI BsiYI BslI BsmFI
BsoBI Bsp1286I BsrI BssKI BstF5I
BstNI BstYI Cac8I CfoI Cfr13I
DdeI DpnII DraII DsaI EaeI
EcoO109I EcoRII EcoT14I Fnu4HI FokI
HaeIII HapII HgaI HhaI HinP1I
HinfI HpaII HphI ItaI MaeIII
MboI MflI MluNI MnlI MscI
MspA1I MspI MvaI MwoI NciI
NdeII NlaIII NlaIV PleI PvuII
Sau3AI Sau96I ScrFI SfaNI StyI
TaqI TseI TspRI TthHB8I XcmI
XhoII