(Circular) (Six-base) MAPSORT of: pFN21AB0980.seq Check: 8555 from: 1 to: 6055 pFN21AB0980 6055 bp With 182 enzymes: SgfI * April 15, 2010 09:33 .. AatII G_ACGT'C Cuts at: 278 331 414 600 1536 3865 278 Size: 53 83 186 936 2329 2468 Fragments arranged by size: 2468 2329 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 2109 3398 1937 Size: 172 1289 4594 Fragments arranged by size: 4594 1289 172 Acc65I G'GTAC_C Cuts at: 3376 3376 Size: 6055 AclI AA'CG_TT Cuts at: 1563 4182 4555 1563 Size: 2619 373 3063 Fragments arranged by size: 3063 2619 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 2671 4118 5502 5770 1632 Size: 1039 1447 1384 268 1917 Fragments arranged by size: 1917 1447 1384 1039 268 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 5858 AflIII A'CryG_T Cuts at: 1203 4954 5931 1203 Size: 3751 977 1327 Fragments arranged by size: 3751 1327 977 AgeI A'CCGG_T Cuts at: 4886 4886 Size: 6055 AhdI GACnn_n'nnGTC Cuts at: 4784 4784 Size: 6055 AleI CACnn'nnGTG Cuts at: 1166 2124 1166 Size: 958 5097 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 2096 1184 Size: 912 5143 AlwNI CAG_nnn'CTG Cuts at: 3798 5370 3798 Size: 1572 4483 ApaI G_GGCC'C Cuts at: 3111 3111 Size: 6055 ApaLI G'TGCA_C Cuts at: 3318 4112 5268 3318 Size: 794 1156 4105 Fragments arranged by size: 4105 1156 794 ApoI r'AATT_y Cuts at: 1493 3364 3590 3742 5851 1493 Size: 1871 226 152 2109 1697 Fragments arranged by size: 2109 1871 1697 226 152 AseI AT'TA_AT Cuts at: 160 1032 2388 4609 160 Size: 872 1356 2221 1606 Fragments arranged by size: 2221 1606 1356 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 6055 AvaI C'yCGr_G Cuts at: 1870 1943 2702 2711 2939 3380 1870 Size: 73 759 9 228 441 4545 Fragments arranged by size: 4545 759 441 228 73 9 BamHI G'GATC_C Cuts at: 3385 3752 3385 Size: 367 5688 BanI G'GyrC_C Cuts at: 618 943 1160 1787 3376 4831 618 Size: 325 217 627 1589 1455 1842 Fragments arranged by size: 1842 1589 1455 627 325 217 BanII G_rGCy'C Cuts at: 1391 2240 2537 2559 3111 3374 1391 Size: 849 297 22 552 263 4072 Fragments arranged by size: 4072 849 552 297 263 22 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 928 Size: 971 5084 BbvCI CC'TCA_GC Cuts at: 2343 2343 Size: 6055 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 1987 2021 4246 4280 1903 Size: 34 50 34 2225 34 3678 Fragments arranged by size: 3678 2225 50 34 34 34 BciVI GTATCCnnnnn_n' Cuts at: 3116 3947 5157 3116 Size: 831 1210 4014 Fragments arranged by size: 4014 1210 831 BclI T'GATC_A Cuts at: 1548 2608 1548 Size: 1060 4995 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 2860 4666 136 Size: 107 122 71 631 605 1188 1806 1525 Fragments arranged by size: 1806 1525 1188 631 605 122 107 71 BglII A'GATC_T Cuts at: 2024 6050 2024 Size: 4026 2029 BlpI GC'TnA_GC Cuts at: 2491 2571 3472 2491 Size: 80 901 5074 Fragments arranged by size: 5074 901 80 Bme1580I G_kGCm'C Cuts at: 1790 2373 2720 3111 3322 4116 5272 1790 Size: 583 347 391 211 794 1156 2573 Fragments arranged by size: 2573 1156 794 583 391 347 211 BmgBI CAC'GTC Cuts at: 1318 1318 Size: 6055 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 2367 2512 2867 Size: 416 53 83 399 24 950 145 355 Cuts at: 2867 3786 4744 442 Size: 919 958 1753 Fragments arranged by size: 1753 958 950 919 416 399 355 145 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 6055 BplI GAGnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 2351 2351 Size: 6055 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2244 3253 4715 1239 Size: 1005 1009 1462 2579 Fragments arranged by size: 2579 1462 1009 1005 Bpu10I CC'TnA_GC Cuts at: 2343 2343 Size: 6055 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 4180 5045 5343 4180 Size: 865 298 4892 Fragments arranged by size: 4892 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 2322 4718 882 Size: 617 823 2396 2219 Fragments arranged by size: 2396 2219 823 617 BsaAI yAC'GTr Cuts at: 493 1204 3846 493 Size: 711 2642 2702 Fragments arranged by size: 2702 2642 711 BsaBI GATnn'nnATC Cuts at: 6049 6049 Size: 6055 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 2349 3862 4244 4879 Size: 53 83 186 936 816 1513 382 635 Cuts at: 4879 275 Size: 1451 Fragments arranged by size: 1513 1451 936 816 635 382 186 83 53 BsaWI w'CCGG_w Cuts at: 2400 4488 4886 5160 5307 5718 2400 Size: 2088 398 274 147 411 2737 Fragments arranged by size: 2737 2088 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 1178 Size: 30 165 30 5830 Fragments arranged by size: 5830 165 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 3074 3157 3226 1186 Size: 1888 83 69 4015 Fragments arranged by size: 4015 1888 83 69 BseYI C'CCAG_C Cuts at: 2050 2486 2759 5258 5754 2050 Size: 436 273 2499 496 2351 Fragments arranged by size: 2499 2351 496 436 273 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 2432 1163 Size: 573 696 4786 Fragments arranged by size: 4786 696 573 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 3505 4266 5294 1540 Size: 275 186 1504 761 1028 2301 Fragments arranged by size: 2301 1504 1028 761 275 186 BsiHKAI G_wGCw'C Cuts at: 3322 4116 4201 5272 3322 Size: 794 85 1071 4105 Fragments arranged by size: 4105 1071 794 85 BsmI GAATG_Cn' Cuts at: 3566 3659 3566 Size: 93 5962 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2240 2373 2537 2559 2720 3111 3322 Size: 399 450 133 164 22 161 391 211 Cuts at: 3322 3374 4116 4201 5272 1391 Size: 52 742 85 1071 2174 Fragments arranged by size: 2174 1071 742 450 399 391 211 164 161 133 85 52 22 BspEI T'CCGG_A Cuts at: 2400 5718 2400 Size: 3318 2737 BspHI T'CATG_A Cuts at: 3944 5674 3944 Size: 1730 4325 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 2184 3410 844 Size: 1340 1226 3489 Fragments arranged by size: 3489 1340 1226 BsrBI CCG'CTC Cuts at: 1394 1823 2710 2728 3942 1394 Size: 429 887 18 1214 3507 Fragments arranged by size: 3507 1214 887 429 18 BsrDI GCAATG_nn' Cuts at: 65 1248 3217 4550 4724 65 Size: 1183 1969 1333 174 1396 Fragments arranged by size: 1969 1396 1333 1183 174 BsrFI r'CCGG_y Cuts at: 1700 1810 2331 4699 4886 1700 Size: 110 521 2368 187 2869 Fragments arranged by size: 2869 2368 521 187 110 BsrGI T'GTAC_A Cuts at: 96 96 Size: 6055 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 6055 BssSI C'ACGA_G Cuts at: 4115 5127 4115 Size: 1012 5043 BstAPI GCAn_nnn'nTGC Cuts at: 794 794 Size: 6055 BstBI TT'CG_AA Cuts at: 4859 4859 Size: 6055 BstEII G'GTnAC_C Cuts at: 1184 1184 Size: 6055 BstXI CCAn_nnnn'nTGG Cuts at: 2269 2269 Size: 6055 BstYI r'GATC_y Cuts at: 1972 2024 2184 2322 2902 2995 3385 3752 4137 Size: 52 160 138 580 93 390 367 385 Cuts at: 4137 4154 5606 5692 5704 6050 1972 Size: 17 1452 86 12 346 1977 Fragments arranged by size: 1977 1452 580 390 385 367 346 160 138 93 86 52 17 12 BtgI C'CryG_G Cuts at: 513 1065 2032 2167 2892 3336 513 Size: 552 967 135 725 444 3232 Fragments arranged by size: 3232 967 725 552 444 135 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 2551 3160 487 Size: 683 56 1325 609 3382 Fragments arranged by size: 3382 1325 683 609 56 BtsI GCAGTG_nn' Cuts at: 3330 3571 4365 4385 3330 Size: 241 794 20 5000 Fragments arranged by size: 5000 794 241 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 6055 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 3325 3360 529 Size: 35 2761 35 3224 Fragments arranged by size: 3224 2761 35 35 DraI TTT'AAA Cuts at: 3359 3359 Size: 6055 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 5062 809 Size: 507 3746 1802 Fragments arranged by size: 3746 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2029 2253 2448 Size: 54 1425 50 275 18 199 224 195 Cuts at: 2448 3220 3502 4391 6020 8 Size: 772 282 889 1629 43 Fragments arranged by size: 1629 1425 889 772 282 275 224 199 195 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 3502 1537 Size: 275 1690 4090 Fragments arranged by size: 4090 1690 275 EarI CTCTTCn'nnn_ Cuts at: 1353 3985 1353 Size: 2632 3423 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 2084 2974 4642 5016 5162 387 Size: 1064 138 495 890 1668 374 146 1280 Fragments arranged by size: 1668 1280 1064 890 495 374 146 138 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2244 2671 3253 4118 4715 5502 5770 Size: 393 612 427 582 865 597 787 268 Cuts at: 5770 1239 Size: 1524 Fragments arranged by size: 1524 865 787 612 597 582 427 393 268 EcoNI CCTnn'n_nnAGG Cuts at: 2342 2342 Size: 6055 EcoO109I rG'GnC_Cy Cuts at: 2058 2133 2782 3019 3079 3107 2058 Size: 75 649 237 60 28 5006 Fragments arranged by size: 5006 649 237 75 60 28 EcoRI G'AATT_C Cuts at: 3364 3742 3364 Size: 378 5677 FspI TGC'GCA Cuts at: 4561 4561 Size: 6055 HaeII r_GCGC'y Cuts at: 1678 3259 5202 1678 Size: 1581 1943 2531 Fragments arranged by size: 2531 1943 1581 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2894 2926 4696 4728 4770 Size: 32 135 32 1322 32 1770 32 42 Cuts at: 4770 4802 1373 Size: 32 2626 Fragments arranged by size: 2626 1770 1322 135 42 32 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 3399 3645 669 Size: 988 281 1461 246 3079 Fragments arranged by size: 3079 1461 988 281 246 HindIII A'AGCT_T Cuts at: 748 748 Size: 6055 HpaI GTT'AAC Cuts at: 3645 3645 Size: 6055 KpnI G_GTAC'C Cuts at: 3380 3380 Size: 6055 MfeI C'AATT_G Cuts at: 3654 3654 Size: 6055 MluI A'CGCG_T Cuts at: 5931 5931 Size: 6055 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 2502 2871 5169 5353 1305 Size: 1197 369 2298 184 2007 Fragments arranged by size: 2298 2007 1197 369 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 2031 2255 2450 3222 10 Size: 54 1425 343 199 224 195 772 2843 Fragments arranged by size: 2843 1425 772 343 224 199 195 54 MslI CAynn'nnrTG Cuts at: 518 1166 2124 4013 4372 4531 518 Size: 648 958 1889 359 159 2042 Fragments arranged by size: 2042 1889 958 648 359 159 MspA1I CmG'CkG Cuts at: 1606 2050 2442 2495 2655 2794 3147 3471 4148 Size: 444 392 53 160 139 353 324 677 Cuts at: 4148 5296 5541 1606 Size: 1148 245 2120 Fragments arranged by size: 2120 1148 677 444 392 353 324 245 160 139 53 NcoI C'CATG_G Cuts at: 513 1065 2032 2167 513 Size: 552 967 135 4401 Fragments arranged by size: 4401 967 552 135 NdeI CA'TA_TG Cuts at: 387 387 Size: 6055 NheI G'CTAG_C Cuts at: 1052 1052 Size: 6055 NotI GC'GGCC_GC Cuts at: 3502 3502 Size: 6055 NspI r_CATG'y Cuts at: 1132 1229 3413 4958 1132 Size: 97 2184 1545 2229 Fragments arranged by size: 2229 2184 1545 97 PasI CC'CwG_GG Cuts at: 1348 2530 1348 Size: 1182 4873 PciI A'CATG_T Cuts at: 4954 4954 Size: 6055 PflMI CCAn_nnn'nTGG Cuts at: 1108 1108 Size: 6055 PfoI T'CCnGG_A Cuts at: 2467 2467 Size: 6055 PmeI GTTT'AAAC Cuts at: 3359 3359 Size: 6055 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 4124 4156 5637 5669 4124 Size: 32 1481 32 4510 Fragments arranged by size: 4510 1481 32 32 PpuMI rG'GwC_Cy Cuts at: 3019 3019 Size: 6055 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 6055 PsiI TTA'TAA Cuts at: 3625 3625 Size: 6055 PspOMI G'GGCC_C Cuts at: 3107 3107 Size: 6055 PspXI vC'TCGA_Gb Cuts at: 2702 2711 2702 Size: 9 6046 PsrI GAACnnnnnnTACnnnnnnn_nnnnn' Cuts at: 3050 3082 3050 Size: 32 6023 PstI C_TGCA'G Cuts at: 830 3003 3407 830 Size: 2173 404 3478 Fragments arranged by size: 3478 2173 404 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 6055 PvuII CAG'CTG Cuts at: 2050 2442 2495 2050 Size: 392 53 5610 Fragments arranged by size: 5610 392 53 SalI G'TCGA_C Cuts at: 1936 3397 1936 Size: 1461 4594 SanDI GG'GwC_CC Cuts at: 3019 3019 Size: 6055 SbfI CC_TGCA'GG Cuts at: 3407 3407 Size: 6055 ScaI AGT'ACT Cuts at: 4303 6015 4303 Size: 1712 4343 SfcI C'TryA_G Cuts at: 826 1045 2999 3241 3403 4538 5219 5410 826 Size: 219 1954 242 162 1135 681 191 1471 Fragments arranged by size: 1954 1471 1135 681 242 219 191 162 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 6055 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 6055 SmaI CCC'GGG Cuts at: 1872 3382 1872 Size: 1510 4545 SmlI C'TyrA_G Cuts at: 820 1017 1943 2702 2711 4159 5060 5322 820 Size: 197 926 759 9 1448 901 262 1553 Fragments arranged by size: 1553 1448 926 901 759 262 197 9 SnaBI TAC'GTA Cuts at: 493 3846 493 Size: 3353 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 6055 SphI G_CATG'C Cuts at: 1132 3413 1132 Size: 2281 3774 SspI AAT'ATT Cuts at: 5 52 3979 5 Size: 47 3927 2081 Fragments arranged by size: 3927 2081 47 StuI AGG'CCT Cuts at: 1517 2747 3251 1517 Size: 1230 504 4321 Fragments arranged by size: 4321 1230 504 StyI C'CwwG_G Cuts at: 513 1065 2032 2167 2188 2247 2262 3494 513 Size: 552 967 135 21 59 15 1232 3074 Fragments arranged by size: 3074 1232 967 552 135 59 21 15 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 4082 4099 4252 4437 4882 325 Size: 1255 2502 17 153 185 445 1498 Fragments arranged by size: 2502 1498 1255 445 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 4301 6013 96 Size: 274 80 33 51 1443 2324 1712 138 Fragments arranged by size: 2324 1712 1443 274 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 3120 3152 6015 6047 3120 Size: 32 2863 32 3128 Fragments arranged by size: 3128 2863 32 32 Tth111I GACn'n_nGTC Cuts at: 1316 1316 Size: 6055 XbaI T'CTAG_A Cuts at: 3391 3391 Size: 6055 XcmI CCAnnnn_n'nnnnTGG Cuts at: 2248 2263 2854 3181 2248 Size: 15 591 327 5122 Fragments arranged by size: 5122 591 327 15 XhoI C'TCGA_G Cuts at: 1943 2702 2711 1943 Size: 759 9 5287 Fragments arranged by size: 5287 759 9 XmaI C'CCGG_G Cuts at: 1870 3380 1870 Size: 1510 4545 XmnI GAAnn'nnTTC Cuts at: 4184 4184 Size: 6055 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 3863 276 Size: 53 83 186 936 2329 2468 Fragments arranged by size: 2468 2329 936 186 83 53 Enzymes that do cut: AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbsI BbvCI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BplI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI EarI EciI Eco57MI EcoNI EcoO109I EcoRI FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I NcoI NdeI NheI NotI NspI PasI PciI PflMI PfoI PmeI PpiI PpuMI PshAI PsiI PspOMI PspXI PsrI PstI PvuI PvuII SalI SanDI SbfI ScaI SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI Enzymes that do not cut: AarI AfeI AloI AscI AvrII BaeI BbeI BfrBI BsiWI BsmBI BstZ17I Bsu36I DraIII EcoICRI EcoRV FalI FseI FspAI KasI NaeI NarI NgoMIV NruI NsiI PacI PmlI RsrII SacI SacII SapI SexAI SfiI SfoI SrfI SwaI