Restriction map for
pFN21AB0980
(Linear) (Six-base) MAPSORT of: KIBB0980.seq Check: 1587 from: 1 to: 1350
KIBB0980.orf
With 182 enzymes: SgfI *
October 7, 2009 14:15 ..
AccI GT'mk_AC
Cuts at: 0 104 1350
Size: 104 1246
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 666 1350
Size: 666 684
AleI CACnn'nnGTG
Cuts at: 0 119 1350
Size: 119 1231
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 91 1350
Size: 91 1259
ApaI G_GGCC'C
Cuts at: 0 1106 1350
Size: 1106 244
ApaLI G'TGCA_C
Cuts at: 0 1313 1350
Size: 1313 37
AseI AT'TA_AT
Cuts at: 0 383 1350
Size: 383 967
AvaI C'yCGr_G
Cuts at: 0 697 706 934 1350
Size: 697 9 228 416
Fragments arranged by size:
697 416 228 9
BanII G_rGCy'C
Cuts at: 0 235 532 554 1106 1350
Size: 235 297 22 552 244
Fragments arranged by size:
552 297 244 235 22
BbvCI CC'TCA_GC
Cuts at: 0 338 1350
Size: 338 1012
BciVI GTATCCnnnnn_n'
Cuts at: 0 1111 1350
Size: 1111 239
BclI T'GATC_A
Cuts at: 0 603 1350
Size: 603 747
BglI GCCn_nnn'nGGC
Cuts at: 0 855 1350
Size: 855 495
BglII A'GATC_T
Cuts at: 0 19 1350
Size: 19 1331
BlpI GC'TnA_GC
Cuts at: 0 486 566 1350
Size: 486 80 784
Fragments arranged by size:
784 486 80
Bme1580I G_kGCm'C
Cuts at: 0 368 715 1106 1317 1350
Size: 368 347 391 211 33
Fragments arranged by size:
391 368 347 211 33
BmrI ACTGGGnnnn_n'
Cuts at: 0 362 507 862 1350
Size: 362 145 355 488
Fragments arranged by size:
488 362 355 145
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 346 1350
Size: 346 1004
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 239 1248 1350
Size: 239 1009 102
Fragments arranged by size:
1009 239 102
Bpu10I CC'TnA_GC
Cuts at: 0 338 1350
Size: 338 1012
BsaI GGTCTCn'nnnn_
Cuts at: 0 317 1350
Size: 317 1033
BsaHI Gr'CG_yC
Cuts at: 0 344 1350
Size: 344 1006
BsaWI w'CCGG_w
Cuts at: 0 395 1350
Size: 395 955
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 1069 1152 1221 1350
Size: 1069 83 69 129
Fragments arranged by size:
1069 129 83 69
BseYI C'CCAG_C
Cuts at: 0 45 481 754 1350
Size: 45 436 273 596
Fragments arranged by size:
596 436 273 45
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 427 1350
Size: 427 923
BsiHKAI G_wGCw'C
Cuts at: 0 1317 1350
Size: 1317 33
Bsp1286I G_dGCh'C
Cuts at: 0 235 368 532 554 715 1106 1317 1350
Size: 235 133 164 22 161 391 211 33
Fragments arranged by size:
391 235 211 164 161 133 33 22
BspEI T'CCGG_A
Cuts at: 0 395 1350
Size: 395 955
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 179 1350
Size: 179 1171
BsrBI CCG'CTC
Cuts at: 0 705 723 1350
Size: 705 18 627
Fragments arranged by size:
705 627 18
BsrDI GCAATG_nn'
Cuts at: 0 1212 1350
Size: 1212 138
BsrFI r'CCGG_y
Cuts at: 0 326 1350
Size: 326 1024
BstXI CCAn_nnnn'nTGG
Cuts at: 0 264 1350
Size: 264 1086
BstYI r'GATC_y
Cuts at: 0 19 179 317 897 990 1350
Size: 19 160 138 580 93 360
Fragments arranged by size:
580 360 160 138 93 19
BtgI C'CryG_G
Cuts at: 0 27 162 887 1331 1350
Size: 27 135 725 444 19
Fragments arranged by size:
725 444 135 27 19
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 0 546 1155 1350
Size: 546 609 195
Fragments arranged by size:
609 546 195
BtsI GCAGTG_nn'
Cuts at: 0 1325 1350
Size: 1325 25
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 0 1320 1350
Size: 1320 30
EaeI y'GGCC_r
Cuts at: 0 24 248 443 1215 1350
Size: 24 224 195 772 135
Fragments arranged by size:
772 224 195 135 24
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 0 79 969 1350
Size: 79 890 381
Fragments arranged by size:
890 381 79
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 239 666 1248 1350
Size: 239 427 582 102
Fragments arranged by size:
582 427 239 102
EcoNI CCTnn'n_nnAGG
Cuts at: 0 337 1350
Size: 337 1013
EcoO109I rG'GnC_Cy
Cuts at: 0 53 128 777 1014 1074 1102 1350
Size: 53 75 649 237 60 28 248
Fragments arranged by size:
649 248 237 75 60 53 28
HaeII r_GCGC'y
Cuts at: 0 1254 1350
Size: 1254 96
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 889 921 1350
Size: 889 32 429
Fragments arranged by size:
889 429 32
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 497 866 1350
Size: 497 369 484
Fragments arranged by size:
497 484 369
MscI TGG'CCA
Cuts at: 0 26 250 445 1217 1350
Size: 26 224 195 772 133
Fragments arranged by size:
772 224 195 133 26
MslI CAynn'nnrTG
Cuts at: 0 119 1350
Size: 119 1231
MspA1I CmG'CkG
Cuts at: 0 45 437 490 650 789 1142 1350
Size: 45 392 53 160 139 353 208
Fragments arranged by size:
392 353 208 160 139 53 45
NcoI C'CATG_G
Cuts at: 0 27 162 1350
Size: 27 135 1188
Fragments arranged by size:
1188 135 27
PasI CC'CwG_GG
Cuts at: 0 525 1350
Size: 525 825
PfoI T'CCnGG_A
Cuts at: 0 462 1350
Size: 462 888
PpuMI rG'GwC_Cy
Cuts at: 0 1014 1350
Size: 1014 336
PspOMI G'GGCC_C
Cuts at: 0 1102 1350
Size: 1102 248
PspXI vC'TCGA_Gb
Cuts at: 0 697 706 1350
Size: 697 9 644
Fragments arranged by size:
697 644 9
PsrI GAACnnnnnnTACnnnnnnn_nnnnn'
Cuts at: 0 1045 1077 1350
Size: 1045 32 273
Fragments arranged by size:
1045 273 32
PstI C_TGCA'G
Cuts at: 0 998 1350
Size: 998 352
PvuII CAG'CTG
Cuts at: 0 45 437 490 1350
Size: 45 392 53 860
Fragments arranged by size:
860 392 53 45
SanDI GG'GwC_CC
Cuts at: 0 1014 1350
Size: 1014 336
SfcI C'TryA_G
Cuts at: 0 994 1236 1350
Size: 994 242 114
Fragments arranged by size:
994 242 114
SmlI C'TyrA_G
Cuts at: 0 697 706 1350
Size: 697 9 644
Fragments arranged by size:
697 644 9
StuI AGG'CCT
Cuts at: 0 742 1246 1350
Size: 742 504 104
Fragments arranged by size:
742 504 104
StyI C'CwwG_G
Cuts at: 0 27 162 183 242 257 1350
Size: 27 135 21 59 15 1093
Fragments arranged by size:
1093 135 59 27 21 15
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 1115 1147 1350
Size: 1115 32 203
Fragments arranged by size:
1115 203 32
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 243 258 849 1176 1350
Size: 243 15 591 327 174
Fragments arranged by size:
591 327 243 174 15
XhoI C'TCGA_G
Cuts at: 0 697 706 1350
Size: 697 9 644
Fragments arranged by size:
697 644 9
Enzymes that do cut:
AccI AcuI AleI AlfI ApaI ApaLI AseI AvaI
BanII BbvCI BciVI BclI BglI BglII BlpI Bme1580I
BmrI BplI BpmI Bpu10I BsaI BsaHI BsaWI BseRI
BseYI BsgI BsiHKAI Bsp1286I BspEI BspMI BsrBI BsrDI
BsrFI BstXI BstYI BtgI BtgZI BtsI CspCI EaeI
EciI Eco57MI EcoNI EcoO109I HaeII Hin4I MmeI MscI
MslI MspA1I NcoI PasI PfoI PpuMI PspOMI PspXI
PsrI PstI PvuII SanDI SfcI SmlI StuI StyI
TstI XcmI XhoI
Enzymes that do not cut:
AarI AatII Acc65I AclI AfeI AflII AflIII AgeI
AhdI AloI AlwNI ApoI AscI AsiSI AvrII BaeI
BamHI BanI BbeI BbsI BcgI BfrBI BmgBI BmtI
BpuEI BsaAI BsaBI BsaXI BsiEI BsiWI BsmI BsmBI
BspHI BsrGI BssHII BssSI BstAPI BstBI BstEII BstZ17I
Bsu36I ClaI DraI DraIII DrdI EagI EarI EcoICRI
EcoRI EcoRV FalI FseI FspI FspAI HincII HindIII
HpaI KasI KpnI MfeI MluI NaeI NarI NdeI
NgoMIV NheI NotI NruI NsiI NspI PacI PciI
PflMI PmeI PmlI PpiI PshAI PsiI PvuI RsrII
SacI SacII SalI SapI SbfI ScaI SexAI SfiI
SfoI SgfI SgrAI SmaI SnaBI SpeI SphI SrfI
SspI SwaI TaqII TatI Tth111I XbaI XmaI XmnI
ZraI