(Linear) (Six-base) MAPSORT of: KIEE1459.seq Check: 7655 from: 1 to: 3225 KIEE1459.orf With 182 enzymes: SgfI * July 15, 2010 18:39 .. AarI CACCTGCnnnn'nnnn_ Cuts at: 0 2019 3225 Size: 2019 1206 AclI AA'CG_TT Cuts at: 0 2079 3225 Size: 2079 1146 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 0 857 1052 1367 2123 2824 3225 Size: 857 195 315 756 701 401 Fragments arranged by size: 857 756 701 401 315 195 AflIII A'CryG_T Cuts at: 0 3128 3225 Size: 3128 97 AleI CACnn'nnGTG Cuts at: 0 830 3225 Size: 830 2395 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 0 848 3225 Size: 848 2377 AlwNI CAG_nnn'CTG Cuts at: 0 2695 2764 2875 3225 Size: 2695 69 111 350 Fragments arranged by size: 2695 350 111 69 ApaLI G'TGCA_C Cuts at: 0 2032 3225 Size: 2032 1193 ApoI r'AATT_y Cuts at: 0 415 1565 2476 2571 3225 Size: 415 1150 911 95 654 Fragments arranged by size: 1150 911 654 415 95 AseI AT'TA_AT Cuts at: 0 2456 3225 Size: 2456 769 AvaI C'yCGr_G Cuts at: 0 84 1103 3183 3225 Size: 84 1019 2080 42 Fragments arranged by size: 2080 1019 84 42 BamHI G'GATC_C Cuts at: 0 616 3225 Size: 616 2609 BanI G'GyrC_C Cuts at: 0 19 66 72 3225 Size: 19 47 6 3153 Fragments arranged by size: 3153 47 19 6 BanII G_rGCy'C Cuts at: 0 35 91 769 1335 1974 3224 3225 Size: 35 56 678 566 639 1250 1 Fragments arranged by size: 1250 678 639 566 56 35 1 BbeI G_GCGC'C Cuts at: 0 23 70 76 3225 Size: 23 47 6 3149 Fragments arranged by size: 3149 47 23 6 BbsI GAAGACnn'nnnn_ Cuts at: 0 470 1516 2129 2390 2440 3225 Size: 470 1046 613 261 50 785 Fragments arranged by size: 1046 785 613 470 261 50 BbvCI CC'TCA_GC Cuts at: 0 2834 3225 Size: 2834 391 BciVI GTATCCnnnnn_n' Cuts at: 0 1151 3225 Size: 1151 2074 BclI T'GATC_A Cuts at: 0 1167 3225 Size: 1167 2058 BfrBI ATG'CAT Cuts at: 0 535 1480 2266 2907 3225 Size: 535 945 786 641 318 Fragments arranged by size: 945 786 641 535 318 BglII A'GATC_T Cuts at: 0 102 2674 3211 3225 Size: 102 2572 537 14 Fragments arranged by size: 2572 537 102 14 BlpI GC'TnA_GC Cuts at: 0 763 3225 Size: 763 2462 Bme1580I G_kGCm'C Cuts at: 0 325 2036 3225 Size: 325 1711 1189 Fragments arranged by size: 1711 1189 325 BmrI ACTGGGnnnn_n' Cuts at: 0 1388 1514 1718 3225 Size: 1388 126 204 1507 Fragments arranged by size: 1507 1388 204 126 BplI GAGnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 0 460 3225 Size: 460 2765 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 0 629 680 813 1221 2672 2876 3225 Size: 629 51 133 408 1451 204 349 Fragments arranged by size: 1451 629 408 349 204 133 51 Bpu10I CC'TnA_GC Cuts at: 0 2834 2868 3225 Size: 2834 34 357 Fragments arranged by size: 2834 357 34 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 0 127 478 1772 2912 3225 Size: 127 351 1294 1140 313 Fragments arranged by size: 1294 1140 351 313 127 BsaAI yAC'GTr Cuts at: 0 926 3225 Size: 926 2299 BsaHI Gr'CG_yC Cuts at: 0 20 67 73 3225 Size: 20 47 6 3152 Fragments arranged by size: 3152 47 20 6 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 0 3146 3176 3225 Size: 3146 30 49 Fragments arranged by size: 3146 49 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 0 2822 3225 Size: 2822 403 BseYI C'CCAG_C Cuts at: 0 850 1775 2007 2781 3225 Size: 850 925 232 774 444 Fragments arranged by size: 925 850 774 444 232 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 0 1631 2047 2945 3225 Size: 1631 416 898 280 Fragments arranged by size: 1631 898 416 280 BsiHKAI G_wGCw'C Cuts at: 0 35 1774 2036 3225 Size: 35 1739 262 1189 Fragments arranged by size: 1739 1189 262 35 BsmI GAATG_Cn' Cuts at: 0 2907 2909 3225 Size: 2907 2 316 Fragments arranged by size: 2907 316 2 BsmBI CGTCTCn'nnnn_ Cuts at: 0 209 2726 2894 3225 Size: 209 2517 168 331 Fragments arranged by size: 2517 331 209 168 Bsp1286I G_dGCh'C Cuts at: 0 35 91 325 769 1335 1774 1974 2036 Size: 35 56 234 444 566 439 200 62 Cuts at: 2036 3224 3225 Size: 1188 1 Fragments arranged by size: 1188 566 444 439 234 200 62 56 35 1 BspHI T'CATG_A Cuts at: 0 1845 3225 Size: 1845 1380 BspMI ACCTGCnnnn'nnnn_ Cuts at: 0 1603 2019 3225 Size: 1603 416 1206 Fragments arranged by size: 1603 1206 416 BsrDI GCAATG_nn' Cuts at: 0 2055 3225 Size: 2055 1170 BsrFI r'CCGG_y Cuts at: 0 69 3225 Size: 69 3156 BsrGI T'GTAC_A Cuts at: 0 1707 2355 2429 3225 Size: 1707 648 74 796 Fragments arranged by size: 1707 796 648 74 BstAPI GCAn_nnn'nTGC Cuts at: 0 2625 3225 Size: 2625 600 BstBI TT'CG_AA Cuts at: 0 1650 3225 Size: 1650 1575 BstEII G'GTnAC_C Cuts at: 0 2274 3225 Size: 2274 951 BstXI CCAn_nnnn'nTGG Cuts at: 0 1262 3225 Size: 1262 1963 BstYI r'GATC_y Cuts at: 0 102 147 616 736 1132 1864 2674 3211 Size: 102 45 469 120 396 732 810 537 Cuts at: 3211 3225 Size: 14 Fragments arranged by size: 810 732 537 469 396 120 102 45 14 Bsu36I CC'TnA_GG Cuts at: 0 780 3225 Size: 780 2445 BtgI C'CryG_G Cuts at: 0 945 2270 3225 Size: 945 1325 955 Fragments arranged by size: 1325 955 945 BtsI GCAGTG_nn' Cuts at: 0 2030 2308 3225 Size: 2030 278 917 Fragments arranged by size: 2030 917 278 ClaI AT'CG_AT Cuts at: 0 1461 3225 Size: 1461 1764 DraI TTT'AAA Cuts at: 0 1148 3225 Size: 1148 2077 DrdI GACnn_nn'nnGTC Cuts at: 0 2444 3225 Size: 2444 781 EaeI y'GGCC_r Cuts at: 0 1218 3225 Size: 1218 2007 EarI CTCTTCn'nnn_ Cuts at: 0 216 516 2117 3225 Size: 216 300 1601 1108 Fragments arranged by size: 1601 1108 300 216 EcoICRI GAG'CTC Cuts at: 0 33 3225 Size: 33 3192 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 0 629 680 813 857 1052 1221 1367 2123 Size: 629 51 133 44 195 169 146 756 Cuts at: 2123 2672 2824 2876 3225 Size: 549 152 52 349 Fragments arranged by size: 756 629 549 349 195 169 152 146 133 52 51 44 EcoNI CCTnn'n_nnAGG Cuts at: 0 792 2867 3225 Size: 792 2075 358 Fragments arranged by size: 2075 792 358 EcoO109I rG'GnC_Cy Cuts at: 0 784 854 3225 Size: 784 70 2371 Fragments arranged by size: 2371 784 70 EcoRV GAT'ATC Cuts at: 0 2650 3225 Size: 2650 575 FalI AAGnnnnnCTTnnnnnnnn_nnnnn' Cuts at: 0 1043 3225 Size: 1043 2182 HaeII r_GCGC'y Cuts at: 0 23 70 76 3225 Size: 23 47 6 3149 Fragments arranged by size: 3149 47 23 6 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 0 109 141 562 594 728 760 2331 2363 Size: 109 32 421 32 134 32 1571 32 Cuts at: 2363 2694 2726 2986 3018 3225 Size: 331 32 260 32 207 Fragments arranged by size: 1571 421 331 260 207 134 109 32 32 32 32 32 32 HincII GTy'rAC Cuts at: 0 2539 3225 Size: 2539 686 KasI G'GCGC_C Cuts at: 0 19 66 72 3225 Size: 19 47 6 3153 Fragments arranged by size: 3153 47 19 6 MfeI C'AATT_G Cuts at: 0 2351 3225 Size: 2351 874 MscI TGG'CCA Cuts at: 0 1220 3225 Size: 1220 2005 MslI CAynn'nnrTG Cuts at: 0 383 563 830 2213 2902 3225 Size: 383 180 267 1383 689 323 Fragments arranged by size: 1383 689 383 323 267 180 MspA1I CmG'CkG Cuts at: 0 1779 2020 3225 Size: 1779 241 1205 Fragments arranged by size: 1779 1205 241 NaeI GCC'GGC Cuts at: 0 71 3225 Size: 71 3154 NarI GG'CG_CC Cuts at: 0 20 67 73 3225 Size: 20 47 6 3152 Fragments arranged by size: 3152 47 20 6 NcoI C'CATG_G Cuts at: 0 945 2270 3225 Size: 945 1325 955 Fragments arranged by size: 1325 955 945 NdeI CA'TA_TG Cuts at: 0 2262 2336 2742 3225 Size: 2262 74 406 483 Fragments arranged by size: 2262 483 406 74 NgoMIV G'CCGG_C Cuts at: 0 69 3225 Size: 69 3156 NsiI A_TGCA'T Cuts at: 0 537 1482 2268 2909 3225 Size: 537 945 786 641 316 Fragments arranged by size: 945 786 641 537 316 NspI r_CATG'y Cuts at: 0 3132 3225 Size: 3132 93 PasI CC'CwG_GG Cuts at: 0 902 3225 Size: 902 2323 PciI A'CATG_T Cuts at: 0 3128 3225 Size: 3128 97 PflMI CCAn_nnn'nTGG Cuts at: 0 193 301 1216 3016 3225 Size: 193 108 915 1800 209 Fragments arranged by size: 1800 915 209 193 108 PpuMI rG'GwC_Cy Cuts at: 0 784 854 3225 Size: 784 70 2371 Fragments arranged by size: 2371 784 70 PstI C_TGCA'G Cuts at: 0 102 600 972 1748 2025 3086 3225 Size: 102 498 372 776 277 1061 139 Fragments arranged by size: 1061 776 498 372 277 139 102 PvuII CAG'CTG Cuts at: 0 2020 3225 Size: 2020 1205 SacI G_AGCT'C Cuts at: 0 35 3225 Size: 35 3190 SanDI GG'GwC_CC Cuts at: 0 854 3225 Size: 854 2371 ScaI AGT'ACT Cuts at: 0 988 3225 Size: 988 2237 SexAI A'CCwGG_T Cuts at: 0 888 1487 3225 Size: 888 599 1738 Fragments arranged by size: 1738 888 599 SfcI C'TryA_G Cuts at: 0 98 391 596 968 1744 2021 3082 3225 Size: 98 293 205 372 776 277 1061 143 Fragments arranged by size: 1061 776 372 293 277 205 143 98 SfoI GGC'GCC Cuts at: 0 21 68 74 3225 Size: 21 47 6 3151 Fragments arranged by size: 3151 47 21 6 SgrAI Cr'CCGG_yG Cuts at: 0 69 3225 Size: 69 3156 SmaI CCC'GGG Cuts at: 0 86 1105 3225 Size: 86 1019 2120 Fragments arranged by size: 2120 1019 86 SmlI C'TyrA_G Cuts at: 0 106 457 1787 2927 3225 Size: 106 351 1330 1140 298 Fragments arranged by size: 1330 1140 351 298 106 StuI AGG'CCT Cuts at: 0 429 959 2184 3225 Size: 429 530 1225 1041 Fragments arranged by size: 1225 1041 530 429 StyI C'CwwG_G Cuts at: 0 612 945 960 1083 1128 2270 2798 3150 Size: 612 333 15 123 45 1142 528 352 Cuts at: 3150 3225 Size: 75 Fragments arranged by size: 1142 612 528 352 333 123 75 45 15 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 0 723 3225 Size: 723 2502 TatI w'GTAC_w Cuts at: 0 986 1549 1707 1811 2217 2355 2429 3225 Size: 986 563 158 104 406 138 74 796 Fragments arranged by size: 986 796 563 406 158 138 104 74 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 0 3146 3178 3225 Size: 3146 32 47 Fragments arranged by size: 3146 47 32 XbaI T'CTAG_A Cuts at: 0 433 3225 Size: 433 2792 XmaI C'CCGG_G Cuts at: 0 84 1103 3225 Size: 84 1019 2122 Fragments arranged by size: 2122 1019 84 XmnI GAAnn'nnTTC Cuts at: 0 2907 3225 Size: 2907 318 Enzymes that do cut: AarI AclI AcuI AflIII AleI AlfI AlwNI ApaLI ApoI AseI AvaI BamHI BanI BanII BbeI BbsI BbvCI BciVI BclI BfrBI BglII BlpI Bme1580I BmrI BplI BpmI Bpu10I BpuEI BsaAI BsaHI BsaXI BseRI BseYI BsgI BsiHKAI BsmI BsmBI Bsp1286I BspHI BspMI BsrDI BsrFI BsrGI BstAPI BstBI BstEII BstXI BstYI Bsu36I BtgI BtsI ClaI DraI DrdI EaeI EarI EcoICRI Eco57MI EcoNI EcoO109I EcoRV FalI HaeII Hin4I HincII KasI MfeI MscI MslI MspA1I NaeI NarI NcoI NdeI NgoMIV NsiI NspI PasI PciI PflMI PpuMI PstI PvuII SacI SanDI ScaI SexAI SfcI SfoI SgrAI SmaI SmlI StuI StyI TaqII TatI TstI XbaI XmaI XmnI Enzymes that do not cut: AatII AccI Acc65I AfeI AflII AgeI AhdI AloI ApaI AscI AsiSI AvrII BaeI BcgI BglI BmgBI BmtI BsaI BsaBI BsaWI BsiEI BsiWI BspEI BsrBI BssHII BssSI BstZ17I BtgZI CspCI DraIII EagI EciI EcoRI FseI FspI FspAI HindIII HpaI KpnI MluI MmeI NheI NotI NruI PacI PfoI PmeI PmlI PpiI PshAI PsiI PspOMI PspXI PsrI PvuI RsrII SacII SalI SapI SbfI SfiI SgfI SnaBI SpeI SphI SrfI SspI SwaI Tth111I XcmI XhoI ZraI