Restriction map for
pFN21AE1453
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# NO CUT ENZYMES ( 78 enzymes) #
##################################
AclI AflII AgeI AhdI AscI
AseI AsnI Asp700I AspEI AviII
AvrII BbeI BbrPI BcgI BciVI
BfrI BglII BlnI BsaAI BsaI
BsiEI BsiWI BsmI BspDI BsrBI
Bst1107I BstBI BstZ17I ClaI CpoI
DraIII DrdI EagI Eam1105I EclXI
Eco52I EcoRI EcoT22I FseI FspI
HpaI KasI MluI NaeI NarI
NcoI NdeI NgoMIV NheI NotI
NruI NsiI NspV PacI PaeR7I
PinAI PmaCI PmeI PmlI PshBI
Psp1406I PvuI RsrII SalI ScaI
SexAI SfiI SfoI SfuI SgrAI
SnaBI SpeI SphI SspI SwaI
XcmI XhoI XmnI
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# ONE CUT ENZYMES ( 45 enzymes) #
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AatII 1383 GACGTC GACGT^C
Acc65I 2678 GGTACC G^GTACC
AcsI 712 AAATTC [GA]^AATT[CT]
AflIII 3019 ACATGT A^C[GA][CT]GT
Alw44I 1212 GTGCAC G^TGCAC
AocI 1020 CCTGAGG CC^T[ACGT]AGG
Aor51HI 374 AGCGCT AGC^GCT
ApaLI 1212 GTGCAC G^TGCAC
ApoI 712 AAATTC [GA]^AATT[CT]
Asp718I 2678 GGTACC G^GTACC
BalI 2624 TGGCCA TGG^CCA
BglI 752 GCCGCCTGGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
BsmBI 1118 GAGACG CGTCTC(1/5)
Bsp1407I 2492 TGTACA T^GTACA
BspLU11I 3019 ACATGT A^CATGT
BsrDI 2291 GCAATG GCAATG(2/0)
BsrGI 2492 TGTACA T^GTACA
BssHII 1334 GCGCGC G^CGCGC
BssSI 109 CACGAG CACGAG(-5/-1)
BstAPI 1806 GCAGGAGCTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
BstEII 999 GGTCACC G^GT[ACGT]ACC
BstPI 999 GGTCACC G^GT[ACGT]ACC
BstXI 1697 CCAAGGAGTTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
Bsu36I 1020 CCTGAGG CC^T[ACGT]AGG
DraI 445 TTTAAA TTT^AAA
DsaI 1193 CCGCGG C^C[GA][CT]GG
Eco47III 374 AGCGCT AGC^GCT
Eco81I 1020 CCTGAGG CC^T[ACGT]AGG
EcoO65I 999 GGTCACC G^GT[ACGT]ACC
EcoRV 2357 GATATC GAT^ATC
HincII 2425 GTCAAC GT[CT]^[GA]AC
HindII 2425 GTCAAC GT[CT]^[GA]AC
HindIII 2755 AAGCTT A^AGCTT
KpnI 2678 GGTACC GGTAC^C
KspI 1193 CCGCGG CCGC^GG
MfeI 2565 CAATTG C^AATTG
MluNI 2624 TGGCCA TGG^CCA
MscI 2624 TGGCCA TGG^CCA
MunI 2565 CAATTG C^AATTG
SacII 1193 CCGCGG CCGC^GG
SmaI 604 CCCGGG CCC^GGG
SspBI 2492 TGTACA T^GTACA
XbaI 351 TCTAGA T^CTAGA
XmaCI 604 CCCGGG C^CCGGG
XmaI 604 CCCGGG C^CCGGG
###################################
# TWO CUT ENZYMES ( 23 enzymes) #
###################################
AccI 139 GTAGAC GT^[AC][GT]AC
1903 GTAGAC
AcyI 1383 GACGTC G[GA]^CG[CT]C
2014 GACGCC
AspI 1249 GACCCGGTC GAC[ACGT]^[ACGT][ACGT]GTC
1474 GACAGAGTC
BamHI 1034 GGATCC G^GATCC
2150 GGATCC
BclI 86 TGATCA T^GATCA
2040 TGATCA
BsaBI 570 GATGCTGATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
2224 GATGCTCATC
BsaHI 1383 GACGTC G[GA]^CG[CT]C
2014 GACGCC
BspHI 3171 TCATGA T^CATGA
3179 TCATGA
BsrFI 936 GCCGGT [GA]^CCGG[CT]
1304 GCCGGT
Cfr10I 936 GCCGGT [GA]^CCGG[CT]
1304 GCCGGT
FbaI 86 TGATCA T^GATCA
2040 TGATCA
HaeII 374 AGCGCT [GA]GCGC^[CT]
894 GGCGCT
Hin1I 1383 GACGTC G[GA]^CG[CT]C
2014 GACGCC
MamI 570 GATGCTGATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
2224 GATGCTCATC
NspI 2048 ACATGC [GA]CATG^[CT]
3019 ACATGT
PflFI 1249 GACCCGGTC GAC[ACGT]^[ACGT][ACGT]GTC
1474 GACAGAGTC
PflMI 546 CCAGGTTATGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
2190 CCAGAGAGTGG
RcaI 3171 TCATGA T^CATGA
3179 TCATGA
SacI 112 GAGCTC GAGCT^C
745 GAGCTC
SapI 516 GAAGAGC GCTCTTC(1/4)
2248 GCTCTTC
StuI 645 AGGCCT AGG^CCT
3160 AGGCCT
Tth111I 1249 GACCCGGTC GAC[ACGT]^[ACGT][ACGT]GTC
1474 GACAGAGTC
Van91I 546 CCAGGTTATGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
2190 CCAGAGAGTGG
#########################################
# 3 OR MORE CUT ENZYMES ( 112 enzymes) #
#########################################
AccII AccIII AciI AfaI AluI
AlwI AlwNI ApaI AspHI AvaI
AvaII BanI BanII BbsI BbvCI
BbvI BcnI BfaI BlpI BmyI
BpmI Bpu1102I BpuAI BsaJI BsaWI
BseAI BseRI BsgI BsiHKAI BsiYI
BslI BsmAI BsmFI BsoBI Bsp1286I
BspEI BspMI BsrI BssKI BstF5I
BstNI BstUI BstYI Cac8I CelII
CfoI Cfr13I DdeI DpnII DraII
EaeI EarI EcoNI EcoO109I EcoRII
EcoT14I FauI Fnu4HI FokI HaeIII
HapII HgaI HhaI HinP1I HinfI
HpaII HphI ItaI Ksp632I MaeI
MaeII MaeIII MboI MboII MflI
MnlI MroI MseI MslI MspA1I
MspI MvaI MvnI MwoI NciI
NdeII NlaIII NlaIV PleI PpuMI
PshAI PstI PvuII RsaI Sau3AI
Sau96I SbfI ScrFI SfaNI SfcI
SmlI Sse8387I StyI TaqI TfiI
Tru9I TseI Tsp45I Tsp509I TspRI
TthHB8I XhoII