(Circular) (Six-base) MAPSORT of: pFN21AE1453.seq Check: 726 from: 1 to: 7891 pFN21AE1453 7891 bp With 182 enzymes: SgfI * August 16, 2010 14:51 .. AarI CACCTGCnnnn'nnnn_ Cuts at: 3275 3275 Size: 7891 AatII G_ACGT'C Cuts at: 278 331 414 600 1536 3392 5701 278 Size: 53 83 186 936 1856 2309 2468 Fragments arranged by size: 2468 2309 1856 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 2145 3909 5234 1937 Size: 208 1764 1325 4594 Fragments arranged by size: 4594 1764 1325 208 Acc65I G'GTAC_C Cuts at: 4683 5212 4683 Size: 529 7362 AclI AA'CG_TT Cuts at: 1563 6018 6391 1563 Size: 4455 373 3063 Fragments arranged by size: 4455 3063 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 2776 3248 5954 7338 7606 1632 Size: 1144 472 2706 1384 268 1917 Fragments arranged by size: 2706 1917 1384 1144 472 268 AfeI AGC'GCT Cuts at: 2381 2381 Size: 7891 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 7694 AflIII A'CryG_T Cuts at: 1203 5024 6790 7767 1203 Size: 3821 1766 977 1327 Fragments arranged by size: 3821 1766 1327 977 AgeI A'CCGG_T Cuts at: 6722 6722 Size: 7891 AhdI GACnn_n'nnGTC Cuts at: 6620 6620 Size: 7891 AleI CACnn'nnGTG Cuts at: 1166 2630 4621 1166 Size: 1464 1991 4436 Fragments arranged by size: 4436 1991 1464 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 3918 1184 Size: 2734 5157 AlwNI CAG_nnn'CTG Cuts at: 2202 2799 3376 3616 3817 4441 5634 7206 2202 Size: 597 577 240 201 624 1193 1572 2887 Fragments arranged by size: 2887 1572 1193 624 597 577 240 201 ApaI G_GGCC'C Cuts at: 2616 2768 3025 2616 Size: 152 257 7482 Fragments arranged by size: 7482 257 152 ApaLI G'TGCA_C Cuts at: 3217 5948 7104 3217 Size: 2731 1156 4004 Fragments arranged by size: 4004 2731 1156 ApoI r'AATT_y Cuts at: 1493 2717 5200 5426 5578 7687 1493 Size: 1224 2483 226 152 2109 1697 Fragments arranged by size: 2483 2109 1697 1224 226 152 AseI AT'TA_AT Cuts at: 160 1032 6445 160 Size: 872 5413 1606 Fragments arranged by size: 5413 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 7891 AvaI C'yCGr_G Cuts at: 1870 1943 2609 3422 4367 4688 5216 1870 Size: 73 666 813 945 321 528 4545 Fragments arranged by size: 4545 945 813 666 528 321 73 BamHI G'GATC_C Cuts at: 3039 4155 5221 5588 3039 Size: 1116 1066 367 5342 Fragments arranged by size: 5342 1116 1066 367 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2944 4683 5036 5212 6667 Size: 325 217 627 1157 1739 353 176 1455 Cuts at: 6667 618 Size: 1842 Fragments arranged by size: 1842 1739 1455 1157 627 353 325 217 176 BanII G_rGCy'C Cuts at: 1391 2121 2616 2754 2768 3025 3191 5210 1391 Size: 730 495 138 14 257 166 2019 4072 Fragments arranged by size: 4072 2019 730 495 257 166 138 14 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 2004 2504 2782 4926 928 Size: 971 105 500 278 2144 3893 Fragments arranged by size: 3893 2144 971 500 278 105 BbvCI CC'TCA_GC Cuts at: 2738 3515 4937 2738 Size: 777 1422 5692 Fragments arranged by size: 5692 1422 777 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 6082 6116 1903 Size: 34 4145 34 3678 Fragments arranged by size: 4145 3678 34 34 BciVI GTATCCnnnnn_n' Cuts at: 5783 6993 5783 Size: 1210 6681 BclI T'GATC_A Cuts at: 1548 2091 4045 1548 Size: 543 1954 5394 Fragments arranged by size: 5394 1954 543 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 2763 6502 136 Size: 107 122 71 631 605 1091 3739 1525 Fragments arranged by size: 3739 1525 1091 631 605 122 107 71 BglII A'GATC_T Cuts at: 7886 7886 Size: 7891 BlpI GC'TnA_GC Cuts at: 2057 2753 3353 5308 2057 Size: 696 600 1955 4640 Fragments arranged by size: 4640 1955 696 600 Bme1580I G_kGCm'C Cuts at: 1790 2616 2768 2949 3025 3221 5952 7108 1790 Size: 826 152 181 76 196 2731 1156 2573 Fragments arranged by size: 2731 2573 1156 826 196 181 152 76 BmgBI CAC'GTC Cuts at: 1318 2362 4446 1318 Size: 1044 2084 4763 Fragments arranged by size: 4763 2084 1044 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 2212 2701 2935 Size: 416 53 83 399 24 795 489 234 Cuts at: 2935 5622 6580 442 Size: 2687 958 1753 Fragments arranged by size: 2687 1753 958 795 489 416 399 234 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 7891 BplI GAGnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 3485 3485 Size: 7891 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2069 2105 2207 2396 2553 3104 3575 4178 Size: 830 36 102 189 157 551 471 603 Cuts at: 4178 6551 1239 Size: 2373 2579 Fragments arranged by size: 2579 2373 830 603 551 471 189 157 102 36 Bpu10I CC'TnA_GC Cuts at: 2738 3515 4937 2738 Size: 777 1422 5692 Fragments arranged by size: 5692 1422 777 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 4357 4699 4891 6016 6881 7179 4357 Size: 342 192 1125 865 298 5069 Fragments arranged by size: 5069 1125 865 342 298 192 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 6554 882 Size: 617 5055 2219 Fragments arranged by size: 5055 2219 617 BsaAI yAC'GTr Cuts at: 493 1204 5682 493 Size: 711 4478 2702 Fragments arranged by size: 4478 2702 711 BsaBI GATnn'nnATC Cuts at: 2579 4233 7885 2579 Size: 1654 3652 2585 Fragments arranged by size: 3652 2585 1654 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 3389 4020 5698 6080 Size: 53 83 186 936 1856 631 1678 382 Cuts at: 6080 6715 275 Size: 635 1451 Fragments arranged by size: 1856 1678 1451 936 635 631 382 186 83 53 BsaWI w'CCGG_w Cuts at: 3036 3623 3739 5043 6324 6722 6996 7143 7554 Size: 587 116 1304 1281 398 274 147 411 Cuts at: 7554 3036 Size: 3373 Fragments arranged by size: 3373 1304 1281 587 411 398 274 147 116 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 2103 2133 3470 3500 1178 Size: 30 165 30 700 30 1337 30 5569 Fragments arranged by size: 5569 1337 700 165 30 30 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 3065 3101 3175 3578 3860 3863 1186 Size: 1879 36 74 403 282 3 5214 Fragments arranged by size: 5214 1879 403 282 74 36 3 BseYI C'CCAG_C Cuts at: 2881 3184 4222 7094 7590 2881 Size: 303 1038 2872 496 3182 Fragments arranged by size: 3182 2872 1038 496 303 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 2503 2791 3449 3878 1163 Size: 573 767 288 658 429 5176 Fragments arranged by size: 5176 767 658 573 429 288 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 5341 6102 7130 1540 Size: 275 186 3340 761 1028 2301 Fragments arranged by size: 3340 2301 1028 761 275 186 BsiHKAI G_wGCw'C Cuts at: 2121 2754 3221 5952 6037 7108 2121 Size: 633 467 2731 85 1071 2904 Fragments arranged by size: 2904 2731 1071 633 467 85 BsmI GAATG_Cn' Cuts at: 5402 5495 5402 Size: 93 7798 BsmBI CGTCTCn'nnnn_ Cuts at: 3117 3117 Size: 7891 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2121 2616 2754 2768 2949 3025 3191 Size: 399 331 495 138 14 181 76 166 Cuts at: 3191 3221 5210 5952 6037 7108 1391 Size: 30 1989 742 85 1071 2174 Fragments arranged by size: 2174 1989 1071 742 495 399 331 181 166 138 85 76 30 14 BspEI T'CCGG_A Cuts at: 3036 3623 5043 7554 3036 Size: 587 1420 2511 3373 Fragments arranged by size: 3373 2511 1420 587 BspHI T'CATG_A Cuts at: 5176 5184 5780 7510 5176 Size: 8 596 1730 5557 Fragments arranged by size: 5557 1730 596 8 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 2043 2475 3275 3816 3960 3961 4365 5246 Size: 1199 432 800 541 144 1 404 881 Cuts at: 5246 844 Size: 3489 Fragments arranged by size: 3489 1199 881 800 541 432 404 144 1 BsrBI CCG'CTC Cuts at: 1394 1823 5778 1394 Size: 429 3955 3507 Fragments arranged by size: 3955 3507 429 BsrDI GCAATG_nn' Cuts at: 65 1248 4303 6386 6560 65 Size: 1183 3055 2083 174 1396 Fragments arranged by size: 3055 2083 1396 1183 174 BsrFI r'CCGG_y Cuts at: 1700 1810 2941 3309 6535 6722 1700 Size: 110 1131 368 3226 187 2869 Fragments arranged by size: 3226 2869 1131 368 187 110 BsrGI T'GTAC_A Cuts at: 96 4497 96 Size: 4401 3490 BssHII G'CGCG_C Cuts at: 1925 3339 1925 Size: 1414 6477 BssSI C'ACGA_G Cuts at: 2114 5951 6963 2114 Size: 3837 1012 3042 Fragments arranged by size: 3837 3042 1012 BstAPI GCAn_nnn'nTGC Cuts at: 794 3817 794 Size: 3023 4868 BstBI TT'CG_AA Cuts at: 6695 6695 Size: 7891 BstEII G'GTnAC_C Cuts at: 1184 3004 1184 Size: 1820 6071 BstXI CCAn_nnnn'nTGG Cuts at: 3709 3709 Size: 7891 BstYI r'GATC_y Cuts at: 1972 2127 2251 3039 3409 4034 4102 4155 4795 Size: 155 124 788 370 625 68 53 640 Cuts at: 4795 5221 5588 5973 5990 7442 7528 7540 7886 Size: 426 367 385 17 1452 86 12 346 Cuts at: 7886 1972 Size: 1977 Fragments arranged by size: 1977 1452 788 640 625 426 385 370 367 346 155 124 86 68 53 17 12 Bsu36I CC'TnA_GG Cuts at: 3026 3026 Size: 7891 BtgI C'CryG_G Cuts at: 513 1065 2004 3198 513 Size: 552 939 1194 5206 Fragments arranged by size: 5206 1194 939 552 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 3778 4752 487 Size: 683 56 2552 974 3626 Fragments arranged by size: 3626 2552 974 683 56 BtsI GCAGTG_nn' Cuts at: 3534 3965 4002 4406 5407 6201 6221 3534 Size: 431 37 404 1001 794 20 5204 Fragments arranged by size: 5204 1001 794 431 404 37 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 7891 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 7856 DraI TTT'AAA Cuts at: 2452 5195 2452 Size: 2743 5148 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 6898 809 Size: 507 5582 1802 Fragments arranged by size: 5582 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 3363 3583 4629 Size: 54 1425 50 275 18 1533 220 1046 Cuts at: 4629 5338 6227 7856 8 Size: 709 889 1629 43 Fragments arranged by size: 1629 1533 1425 1046 889 709 275 220 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 5338 1537 Size: 275 3526 4090 Fragments arranged by size: 4090 3526 275 EarI CTCTTCn'nnn_ Cuts at: 1353 2516 3564 3689 4260 5821 1353 Size: 1163 1048 125 571 1561 3423 Fragments arranged by size: 3423 1561 1163 1048 571 125 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 3062 3461 6478 6852 6998 387 Size: 1064 138 1473 399 3017 374 146 1280 Fragments arranged by size: 3017 1473 1280 1064 399 374 146 138 EcoICRI GAG'CTC Cuts at: 2119 2752 2119 Size: 633 7258 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2069 2105 2207 2396 2553 2776 3104 Size: 393 437 36 102 189 157 223 328 Cuts at: 3104 3248 3575 4178 5954 6551 7338 7606 1239 Size: 144 327 603 1776 597 787 268 1524 Fragments arranged by size: 1776 1524 787 603 597 437 393 328 327 268 223 189 157 144 102 36 EcoNI CCTnn'n_nnAGG Cuts at: 3047 3086 3473 3557 4841 3047 Size: 39 387 84 1284 6097 Fragments arranged by size: 6097 1284 387 84 39 EcoO109I rG'GnC_Cy Cuts at: 2613 3021 3022 3031 3099 3253 3373 4579 2613 Size: 408 1 9 68 154 120 1206 5925 Fragments arranged by size: 5925 1206 408 154 120 68 9 1 EcoRI G'AATT_C Cuts at: 5200 5578 5200 Size: 378 7513 EcoRV GAT'ATC Cuts at: 4364 4364 Size: 7891 FalI AAGnnnnnCTTnnnnnnnn_nnnnn' Cuts at: 5187 5187 Size: 7891 FspI TGC'GCA Cuts at: 6397 6397 Size: 7891 HaeII r_GCGC'y Cuts at: 1678 2383 2903 7038 1678 Size: 705 520 4135 2531 Fragments arranged by size: 4135 2531 705 520 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 3470 3502 4884 4913 4916 Size: 32 135 32 1898 32 1382 29 3 Cuts at: 4916 4945 6532 6564 6606 6638 1373 Size: 29 1587 32 42 32 2626 Fragments arranged by size: 2626 1898 1587 1382 135 42 32 32 32 32 32 29 29 3 HincII GTy'rAC Cuts at: 669 1657 1938 4432 5235 5481 669 Size: 988 281 2494 803 246 3079 Fragments arranged by size: 3079 2494 988 803 281 246 HindIII A'AGCT_T Cuts at: 748 4760 748 Size: 4012 3879 HpaI GTT'AAC Cuts at: 5481 5481 Size: 7891 KpnI G_GTAC'C Cuts at: 4687 5216 4687 Size: 529 7362 MfeI C'AATT_G Cuts at: 4570 5490 4570 Size: 920 6971 MluI A'CGCG_T Cuts at: 7767 7767 Size: 7891 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 3462 3690 4485 4524 7005 7189 1305 Size: 2157 228 795 39 2481 184 2007 Fragments arranged by size: 2481 2157 2007 795 228 184 39 MscI TGG'CCA Cuts at: 10 64 1489 1832 4631 10 Size: 54 1425 343 2799 3270 Fragments arranged by size: 3270 2799 1425 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 2630 3068 3900 4621 5849 6208 6367 Size: 648 1464 438 832 721 1228 359 159 Cuts at: 6367 518 Size: 2042 Fragments arranged by size: 2042 1464 1228 832 721 648 438 359 159 MspA1I CmG'CkG Cuts at: 1606 2742 3184 3200 3331 4226 4941 5307 5984 Size: 1136 442 16 131 895 715 366 677 Cuts at: 5984 7132 7377 1606 Size: 1148 245 2120 Fragments arranged by size: 2120 1148 1136 895 715 677 442 366 245 131 16 NcoI C'CATG_G Cuts at: 513 1065 2004 513 Size: 552 939 6400 Fragments arranged by size: 6400 939 552 NdeI CA'TA_TG Cuts at: 387 387 Size: 7891 NheI G'CTAG_C Cuts at: 1052 1052 Size: 7891 NotI GC'GGCC_GC Cuts at: 5338 5338 Size: 7891 NspI r_CATG'y Cuts at: 1132 1229 4057 5028 5249 6794 1132 Size: 97 2828 971 221 1545 2229 Fragments arranged by size: 2828 2229 1545 971 221 97 PasI CC'CwG_GG Cuts at: 1348 2598 3018 5154 1348 Size: 1250 420 2136 4085 Fragments arranged by size: 4085 2136 1250 420 PciI A'CATG_T Cuts at: 5024 6790 5024 Size: 1766 6125 PflMI CCAn_nnn'nTGG Cuts at: 1108 2557 4201 1108 Size: 1449 1644 4798 Fragments arranged by size: 4798 1644 1449 PfoI T'CCnGG_A Cuts at: 2046 2508 3081 3453 3990 2046 Size: 462 573 372 537 5947 Fragments arranged by size: 5947 573 537 462 372 PmeI GTTT'AAAC Cuts at: 5195 5195 Size: 7891 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 5960 5992 7473 7505 5960 Size: 32 1481 32 6346 Fragments arranged by size: 6346 1481 32 32 PpuMI rG'GwC_Cy Cuts at: 3031 3253 4579 3031 Size: 222 1326 6343 Fragments arranged by size: 6343 1326 222 PshAI GACnn'nnGTC Cuts at: 1613 3444 4185 4971 1613 Size: 1831 741 786 4533 Fragments arranged by size: 4533 1831 786 741 PsiI TTA'TAA Cuts at: 5461 5461 Size: 7891 PspOMI G'GGCC_C Cuts at: 2612 2764 3021 2612 Size: 152 257 7482 Fragments arranged by size: 7482 257 152 PstI C_TGCA'G Cuts at: 830 2040 2650 2747 3237 3308 3813 3957 3972 Size: 1210 610 97 490 71 505 144 15 Cuts at: 3972 4533 4704 5243 830 Size: 561 171 539 3478 Fragments arranged by size: 3478 1210 610 561 539 505 490 171 144 97 71 15 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 7891 PvuII CAG'CTG Cuts at: 2742 3184 3331 4226 4941 2742 Size: 442 147 895 715 5692 Fragments arranged by size: 5692 895 715 442 147 SacI G_AGCT'C Cuts at: 2121 2754 2121 Size: 633 7258 SacII CC_GC'GG Cuts at: 3201 3201 Size: 7891 SalI G'TCGA_C Cuts at: 1936 5233 1936 Size: 3297 4594 SapI GCTCTTCn'nnn_ Cuts at: 2516 4260 2516 Size: 1744 6147 SbfI CC_TGCA'GG Cuts at: 2040 2650 3813 5243 2040 Size: 610 1163 1430 4688 Fragments arranged by size: 4688 1430 1163 610 ScaI AGT'ACT Cuts at: 6139 7851 6139 Size: 1712 6179 SfcI C'TryA_G Cuts at: 826 1045 2036 2646 2743 3233 3304 3809 3953 Size: 219 991 610 97 490 71 505 144 Cuts at: 3953 3968 4529 4700 5239 6374 7055 7246 826 Size: 15 561 171 539 1135 681 191 1471 Fragments arranged by size: 1471 1135 991 681 610 561 539 505 490 219 191 171 144 97 71 15 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 7891 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 7891 SmaI CCC'GGG Cuts at: 1872 2611 5218 1872 Size: 739 2607 4545 Fragments arranged by size: 4545 2607 739 SmlI C'TyrA_G Cuts at: 820 1017 1943 4336 4678 4870 5995 6896 7158 Size: 197 926 2393 342 192 1125 901 262 Cuts at: 7158 820 Size: 1553 Fragments arranged by size: 2393 1553 1125 926 901 342 262 197 192 SnaBI TAC'GTA Cuts at: 493 5682 493 Size: 5189 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 7891 SphI G_CATG'C Cuts at: 1132 5249 1132 Size: 4117 3774 SspI AAT'ATT Cuts at: 5 52 5815 5 Size: 47 5763 2081 Fragments arranged by size: 5763 2081 47 StuI AGG'CCT Cuts at: 1517 2652 5167 1517 Size: 1135 2515 4241 Fragments arranged by size: 4241 2515 1135 StyI C'CwwG_G Cuts at: 513 1065 2004 2653 2988 3702 4416 4707 4983 Size: 552 939 649 335 714 714 291 276 Cuts at: 4983 5330 513 Size: 347 3074 Fragments arranged by size: 3074 939 714 714 649 552 347 335 291 276 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 2194 2201 5918 5935 6088 6273 6718 Size: 1255 614 7 3717 17 153 185 445 Cuts at: 6718 325 Size: 1498 Fragments arranged by size: 3717 1498 1255 614 445 185 153 17 7 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 4497 6137 7849 Size: 274 80 33 51 1443 2520 1640 1712 Cuts at: 7849 96 Size: 138 Fragments arranged by size: 2520 1712 1640 1443 274 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 2103 2135 4147 4179 7851 7883 2103 Size: 32 2012 32 3672 32 2111 Fragments arranged by size: 3672 2111 2012 32 32 32 Tth111I GACn'n_nGTC Cuts at: 1316 3257 3482 1316 Size: 1941 225 5725 Fragments arranged by size: 5725 1941 225 XbaI T'CTAG_A Cuts at: 2356 5227 2356 Size: 2871 5020 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 7891 XmaI C'CCGG_G Cuts at: 1870 2609 5216 1870 Size: 739 2607 4545 Fragments arranged by size: 4545 2607 739 XmnI GAAnn'nnTTC Cuts at: 6020 6020 Size: 7891 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 3390 5699 276 Size: 53 83 186 936 1856 2309 2468 Fragments arranged by size: 2468 2309 1856 936 186 83 53 Enzymes that do cut: AarI AatII AccI Acc65I AclI AcuI AfeI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbsI BbvCI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BplI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI BsmBI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI Bsu36I BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoNI EcoO109I EcoRI EcoRV FalI FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I NcoI NdeI NheI NotI NspI PasI PciI PflMI PfoI PmeI PpiI PpuMI PshAI PsiI PspOMI PstI PvuI PvuII SacI SacII SalI SapI SbfI ScaI SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XhoI XmaI XmnI ZraI Enzymes that do not cut: AloI AscI AvrII BaeI BbeI BfrBI BsiWI BstZ17I DraIII FseI FspAI KasI NaeI NarI NgoMIV NruI NsiI PacI PmlI PspXI PsrI RsrII SanDI SexAI SfiI SfoI SrfI SwaI XcmI