(Linear) (Six-base) MAPSORT of: KIEE1453.seq Check: 4597 from: 1 to: 3186 KIEE1453.orf With 182 enzymes: SgfI * July 15, 2010 18:39 .. AarI CACCTGCnnnn'nnnn_ Cuts at: 0 1270 3186 Size: 1270 1916 AatII G_ACGT'C Cuts at: 0 1387 3186 Size: 1387 1799 AccI GT'mk_AC Cuts at: 0 140 1904 3186 Size: 140 1764 1282 Fragments arranged by size: 1764 1282 140 Acc65I G'GTAC_C Cuts at: 0 2678 3186 Size: 2678 508 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 0 771 1243 3186 Size: 771 472 1943 Fragments arranged by size: 1943 771 472 AfeI AGC'GCT Cuts at: 0 376 3186 Size: 376 2810 AflIII A'CryG_T Cuts at: 0 3019 3186 Size: 3019 167 AleI CACnn'nnGTG Cuts at: 0 625 2616 3186 Size: 625 1991 570 Fragments arranged by size: 1991 625 570 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 0 1913 3186 Size: 1913 1273 AlwNI CAG_nnn'CTG Cuts at: 0 197 794 1371 1611 1812 2436 3186 Size: 197 597 577 240 201 624 750 Fragments arranged by size: 750 624 597 577 240 201 197 ApaI G_GGCC'C Cuts at: 0 611 763 1020 3186 Size: 611 152 257 2166 Fragments arranged by size: 2166 611 257 152 ApaLI G'TGCA_C Cuts at: 0 1212 3186 Size: 1212 1974 ApoI r'AATT_y Cuts at: 0 712 3186 Size: 712 2474 AvaI C'yCGr_G Cuts at: 0 604 1417 2362 2683 3186 Size: 604 813 945 321 503 Fragments arranged by size: 945 813 604 503 321 BamHI G'GATC_C Cuts at: 0 1034 2150 3186 Size: 1034 1116 1036 Fragments arranged by size: 1116 1036 1034 BanI G'GyrC_C Cuts at: 0 939 2678 3031 3186 Size: 939 1739 353 155 Fragments arranged by size: 1739 939 353 155 BanII G_rGCy'C Cuts at: 0 116 611 749 763 1020 1186 3186 Size: 116 495 138 14 257 166 2000 Fragments arranged by size: 2000 495 257 166 138 116 14 BbsI GAAGACnn'nnnn_ Cuts at: 0 499 777 2921 3186 Size: 499 278 2144 265 Fragments arranged by size: 2144 499 278 265 BbvCI CC'TCA_GC Cuts at: 0 733 1510 2932 3186 Size: 733 777 1422 254 Fragments arranged by size: 1422 777 733 254 BclI T'GATC_A Cuts at: 0 86 2040 3186 Size: 86 1954 1146 Fragments arranged by size: 1954 1146 86 BglI GCCn_nnn'nGGC Cuts at: 0 758 3186 Size: 758 2428 BlpI GC'TnA_GC Cuts at: 0 52 748 1348 3186 Size: 52 696 600 1838 Fragments arranged by size: 1838 696 600 52 Bme1580I G_kGCm'C Cuts at: 0 611 763 944 1020 1216 3186 Size: 611 152 181 76 196 1970 Fragments arranged by size: 1970 611 196 181 152 76 BmgBI CAC'GTC Cuts at: 0 357 2441 3186 Size: 357 2084 745 Fragments arranged by size: 2084 745 357 BmrI ACTGGGnnnn_n' Cuts at: 0 207 696 930 3186 Size: 207 489 234 2256 Fragments arranged by size: 2256 489 234 207 BplI GAGnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 0 1480 3186 Size: 1480 1706 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 0 64 100 202 391 548 1099 1570 2173 Size: 64 36 102 189 157 551 471 603 Cuts at: 2173 3186 Size: 1013 Fragments arranged by size: 1013 603 551 471 189 157 102 64 36 Bpu10I CC'TnA_GC Cuts at: 0 733 1510 2932 3186 Size: 733 777 1422 254 Fragments arranged by size: 1422 777 733 254 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 0 2352 2694 2886 3186 Size: 2352 342 192 300 Fragments arranged by size: 2352 342 300 192 BsaBI GATnn'nnATC Cuts at: 0 574 2228 3186 Size: 574 1654 958 Fragments arranged by size: 1654 958 574 BsaHI Gr'CG_yC Cuts at: 0 1384 2015 3186 Size: 1384 631 1171 Fragments arranged by size: 1384 1171 631 BsaWI w'CCGG_w Cuts at: 0 1031 1618 1734 3038 3186 Size: 1031 587 116 1304 148 Fragments arranged by size: 1304 1031 587 148 116 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 0 98 128 1465 1495 3186 Size: 98 30 1337 30 1691 Fragments arranged by size: 1691 1337 98 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 0 1060 1096 1170 1573 1855 1858 3186 Size: 1060 36 74 403 282 3 1328 Fragments arranged by size: 1328 1060 403 282 74 36 3 BseYI C'CCAG_C Cuts at: 0 876 1179 2217 3186 Size: 876 303 1038 969 Fragments arranged by size: 1038 969 876 303 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 0 498 786 1444 1873 3186 Size: 498 288 658 429 1313 Fragments arranged by size: 1313 658 498 429 288 BsiHKAI G_wGCw'C Cuts at: 0 116 749 1216 3186 Size: 116 633 467 1970 Fragments arranged by size: 1970 633 467 116 BsmBI CGTCTCn'nnnn_ Cuts at: 0 1112 3186 Size: 1112 2074 Bsp1286I G_dGCh'C Cuts at: 0 116 611 749 763 944 1020 1186 1216 Size: 116 495 138 14 181 76 166 30 Cuts at: 1216 3186 Size: 1970 Fragments arranged by size: 1970 495 181 166 138 116 76 30 14 BspEI T'CCGG_A Cuts at: 0 1031 1618 3038 3186 Size: 1031 587 1420 148 Fragments arranged by size: 1420 1031 587 148 BspHI T'CATG_A Cuts at: 0 3171 3179 3186 Size: 3171 8 7 Fragments arranged by size: 3171 8 7 BspMI ACCTGCnnnn'nnnn_ Cuts at: 0 38 470 1270 1811 1955 1956 2360 3186 Size: 38 432 800 541 144 1 404 826 Fragments arranged by size: 826 800 541 432 404 144 38 1 BsrDI GCAATG_nn' Cuts at: 0 2298 3186 Size: 2298 888 BsrFI r'CCGG_y Cuts at: 0 936 1304 3186 Size: 936 368 1882 Fragments arranged by size: 1882 936 368 BsrGI T'GTAC_A Cuts at: 0 2492 3186 Size: 2492 694 BssHII G'CGCG_C Cuts at: 0 1334 3186 Size: 1334 1852 BssSI C'ACGA_G Cuts at: 0 109 3186 Size: 109 3077 BstAPI GCAn_nnn'nTGC Cuts at: 0 1812 3186 Size: 1812 1374 BstEII G'GTnAC_C Cuts at: 0 999 3186 Size: 999 2187 BstXI CCAn_nnnn'nTGG Cuts at: 0 1704 3186 Size: 1704 1482 BstYI r'GATC_y Cuts at: 0 122 246 1034 1404 2029 2097 2150 2790 Size: 122 124 788 370 625 68 53 640 Cuts at: 2790 3186 Size: 396 Fragments arranged by size: 788 640 625 396 370 124 122 68 53 Bsu36I CC'TnA_GG Cuts at: 0 1021 3186 Size: 1021 2165 BtgI C'CryG_G Cuts at: 0 1193 3186 Size: 1193 1993 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 0 1773 2747 3186 Size: 1773 974 439 Fragments arranged by size: 1773 974 439 BtsI GCAGTG_nn' Cuts at: 0 1529 1960 1997 2401 3186 Size: 1529 431 37 404 785 Fragments arranged by size: 1529 785 431 404 37 DraI TTT'AAA Cuts at: 0 447 3186 Size: 447 2739 EaeI y'GGCC_r Cuts at: 0 1358 1578 2624 3186 Size: 1358 220 1046 562 Fragments arranged by size: 1358 1046 562 220 EarI CTCTTCn'nnn_ Cuts at: 0 511 1559 1684 2255 3186 Size: 511 1048 125 571 931 Fragments arranged by size: 1048 931 571 511 125 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 0 1057 1456 3186 Size: 1057 399 1730 Fragments arranged by size: 1730 1057 399 EcoICRI GAG'CTC Cuts at: 0 114 747 3186 Size: 114 633 2439 Fragments arranged by size: 2439 633 114 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 0 64 100 202 391 548 771 1099 1243 Size: 64 36 102 189 157 223 328 144 Cuts at: 1243 1570 2173 3186 Size: 327 603 1013 Fragments arranged by size: 1013 603 328 327 223 189 157 144 102 64 36 EcoNI CCTnn'n_nnAGG Cuts at: 0 1042 1081 1468 1552 2836 3186 Size: 1042 39 387 84 1284 350 Fragments arranged by size: 1284 1042 387 350 84 39 EcoO109I rG'GnC_Cy Cuts at: 0 608 1016 1017 1026 1094 1248 1368 2574 Size: 608 408 1 9 68 154 120 1206 Cuts at: 2574 3186 Size: 612 Fragments arranged by size: 1206 612 608 408 154 120 68 9 1 EcoRV GAT'ATC Cuts at: 0 2359 3186 Size: 2359 827 FalI AAGnnnnnCTTnnnnnnnn_nnnnn' Cuts at: 0 3182 3186 Size: 3182 4 HaeII r_GCGC'y Cuts at: 0 378 898 3186 Size: 378 520 2288 Fragments arranged by size: 2288 520 378 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 0 1465 1497 2879 2908 2911 2940 3186 Size: 1465 32 1382 29 3 29 246 Fragments arranged by size: 1465 1382 246 32 29 29 3 HincII GTy'rAC Cuts at: 0 2427 3186 Size: 2427 759 HindIII A'AGCT_T Cuts at: 0 2755 3186 Size: 2755 431 KpnI G_GTAC'C Cuts at: 0 2682 3186 Size: 2682 504 MfeI C'AATT_G Cuts at: 0 2565 3186 Size: 2565 621 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 0 1457 1685 2480 2519 3186 Size: 1457 228 795 39 667 Fragments arranged by size: 1457 795 667 228 39 MscI TGG'CCA Cuts at: 0 2626 3186 Size: 2626 560 MslI CAynn'nnrTG Cuts at: 0 625 1063 1895 2616 3186 Size: 625 438 832 721 570 Fragments arranged by size: 832 721 625 570 438 MspA1I CmG'CkG Cuts at: 0 737 1179 1195 1326 2221 2936 3186 Size: 737 442 16 131 895 715 250 Fragments arranged by size: 895 737 715 442 250 131 16 NspI r_CATG'y Cuts at: 0 2052 3023 3186 Size: 2052 971 163 Fragments arranged by size: 2052 971 163 PasI CC'CwG_GG Cuts at: 0 593 1013 3149 3186 Size: 593 420 2136 37 Fragments arranged by size: 2136 593 420 37 PciI A'CATG_T Cuts at: 0 3019 3186 Size: 3019 167 PflMI CCAn_nnn'nTGG Cuts at: 0 552 2196 3186 Size: 552 1644 990 Fragments arranged by size: 1644 990 552 PfoI T'CCnGG_A Cuts at: 0 41 503 1076 1448 1985 3186 Size: 41 462 573 372 537 1201 Fragments arranged by size: 1201 573 537 462 372 41 PpuMI rG'GwC_Cy Cuts at: 0 1026 1248 2574 3186 Size: 1026 222 1326 612 Fragments arranged by size: 1326 1026 612 222 PshAI GACnn'nnGTC Cuts at: 0 1439 2180 2966 3186 Size: 1439 741 786 220 Fragments arranged by size: 1439 786 741 220 PspOMI G'GGCC_C Cuts at: 0 607 759 1016 3186 Size: 607 152 257 2170 Fragments arranged by size: 2170 607 257 152 PstI C_TGCA'G Cuts at: 0 35 645 742 1232 1303 1808 1952 1967 Size: 35 610 97 490 71 505 144 15 Cuts at: 1967 2528 2699 3186 Size: 561 171 487 Fragments arranged by size: 610 561 505 490 487 171 144 97 71 35 15 PvuII CAG'CTG Cuts at: 0 737 1179 1326 2221 2936 3186 Size: 737 442 147 895 715 250 Fragments arranged by size: 895 737 715 442 250 147 SacI G_AGCT'C Cuts at: 0 116 749 3186 Size: 116 633 2437 Fragments arranged by size: 2437 633 116 SacII CC_GC'GG Cuts at: 0 1196 3186 Size: 1196 1990 SapI GCTCTTCn'nnn_ Cuts at: 0 511 2255 3186 Size: 511 1744 931 Fragments arranged by size: 1744 931 511 SbfI CC_TGCA'GG Cuts at: 0 35 645 1808 3186 Size: 35 610 1163 1378 Fragments arranged by size: 1378 1163 610 35 SfcI C'TryA_G Cuts at: 0 31 641 738 1228 1299 1804 1948 1963 Size: 31 610 97 490 71 505 144 15 Cuts at: 1963 2524 2695 3186 Size: 561 171 491 Fragments arranged by size: 610 561 505 491 490 171 144 97 71 31 15 SmaI CCC'GGG Cuts at: 0 606 3186 Size: 606 2580 SmlI C'TyrA_G Cuts at: 0 2331 2673 2865 3186 Size: 2331 342 192 321 Fragments arranged by size: 2331 342 321 192 StuI AGG'CCT Cuts at: 0 647 3162 3186 Size: 647 2515 24 Fragments arranged by size: 2515 647 24 StyI C'CwwG_G Cuts at: 0 648 983 1697 2411 2702 2978 3186 Size: 648 335 714 714 291 276 208 Fragments arranged by size: 714 714 648 335 291 276 208 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 0 189 196 3186 Size: 189 7 2990 Fragments arranged by size: 2990 189 7 TatI w'GTAC_w Cuts at: 0 2492 3186 Size: 2492 694 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 0 98 130 2142 2174 3186 Size: 98 32 2012 32 1012 Fragments arranged by size: 2012 1012 98 32 32 Tth111I GACn'n_nGTC Cuts at: 0 1252 1477 3186 Size: 1252 225 1709 Fragments arranged by size: 1709 1252 225 XbaI T'CTAG_A Cuts at: 0 351 3186 Size: 351 2835 XmaI C'CCGG_G Cuts at: 0 604 3186 Size: 604 2582 ZraI GAC'GTC Cuts at: 0 1385 3186 Size: 1385 1801 Enzymes that do cut: AarI AatII AccI Acc65I AcuI AfeI AflIII AleI AlfI AlwNI ApaI ApaLI ApoI AvaI BamHI BanI BanII BbsI BbvCI BclI BglI BlpI Bme1580I BmgBI BmrI BplI BpmI Bpu10I BpuEI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiHKAI BsmBI Bsp1286I BspEI BspHI BspMI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstEII BstXI BstYI Bsu36I BtgI BtgZI BtsI DraI EaeI EarI EciI EcoICRI Eco57MI EcoNI EcoO109I EcoRV FalI HaeII Hin4I HincII HindIII KpnI MfeI MmeI MscI MslI MspA1I NspI PasI PciI PflMI PfoI PpuMI PshAI PspOMI PstI PvuII SacI SacII SapI SbfI SfcI SmaI SmlI StuI StyI TaqII TatI TstI Tth111I XbaI XmaI ZraI Enzymes that do not cut: AclI AflII AgeI AhdI AloI AscI AseI AsiSI AvrII BaeI BbeI BcgI BciVI BfrBI BglII BmtI BsaI BsaAI BsiEI BsiWI BsmI BsrBI BstBI BstZ17I ClaI CspCI DraIII DrdI EagI EcoRI FseI FspI FspAI HpaI KasI MluI NaeI NarI NcoI NdeI NgoMIV NheI NotI NruI NsiI PacI PmeI PmlI PpiI PsiI PspXI PsrI PvuI RsrII SalI SanDI ScaI SexAI SfiI SfoI SgfI SgrAI SnaBI SpeI SphI SrfI SspI SwaI XcmI XhoI XmnI