################################## # NO CUT ENZYMES ( 175 enzymes) # ################################## AatII Acc65I AccII AccIII AciI AclI AcyI AfaI AflII AflIII AgeI AhdI Alw44I AlwI AocI Aor51HI ApaI ApaLI AscI AseI AsnI Asp718I AspEI AspI AvaI AviII AvrII BalI BamHI BanI BbeI BbrPI BbsI BbvCI BcgI BciVI BclI BfaI BfrI BglI BglII BlnI BlpI Bpu1102I BpuAI BsaAI BsaBI BsaHI BsaI BsaWI BseAI BsgI BsiEI BsiWI BsiYI BslI BsmAI BsmBI BsoBI Bsp1407I BspDI BspEI BspLU11I BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI Bst1107I BstAPI BstEII BstF5I BstPI BstUI BstXI BstYI BstZ17I Bsu36I CelII CfoI Cfr10I ClaI CpoI DraI DraIII DrdI EaeI EagI Eam1105I EarI EclXI Eco47III Eco52I Eco81I EcoNI EcoO65I EcoRV EcoT22I FauI FbaI FokI FseI FspI HaeII HgaI HhaI Hin1I HinP1I HpaI KasI KpnI Ksp632I KspI MaeI MaeII MamI MflI MluI MluNI MroI MscI MvnI NaeI NarI NdeI NgoMIV NheI NotI NruI NsiI NspI PacI PaeR7I PflFI PflMI PinAI PmaCI PmeI PmlI PshAI PshBI Psp1406I PvuI RsaI RsrII SacI SacII SalI SapI SbfI ScaI SexAI SfiI SfoI SgrAI SmaI SmlI SnaBI SpeI SphI Sse8387I SspBI SspI StuI SwaI Tth111I Van91I XbaI XcmI XhoI XhoII XmaCI XmaI ################################### # ONE CUT ENZYMES ( 45 enzymes) # ################################### AccI 400 GTAGAC GT^[AC][GT]AC AlwNI 310 CAGTGGCTG CAG[ACGT][ACGT][ACGT]^CTG Asp700I 211 GAAAGATTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC AspHI 579 GAGCAC G[AT]GC[AT]^C AvaII 529 GGTCC G^G[AT]CC BanII 248 GAGCCC G[GA]GC[CT]^C BcnI 532 CCCGG CC^[GC]GG BseRI 245 GAGGAG GAGGAG(10/8) BsiHKAI 579 GAGCAC G[AT]GC[AT]^C BsmI 468 GAATGC GAATGC(1/-1) BspHI 494 TCATGA T^CATGA BstBI 218 TTCGAA TT^CGAA BstNI 207 CCAGG CC^[AT]GG Cac8I 202 GCAGGC GC[ACGT]^[ACGT]GC Cfr13I 529 GGTCC G^G[ACGT]CC DraII 528 GGGTCCC [GA]G^G[ACGT]CC[CT] DsaI 252 CCATGG C^C[GA][CT]GG EcoO109I 528 GGGTCCC [GA]G^G[ACGT]CC[CT] EcoRI 440 GAATTC G^AATTC EcoRII 207 CCAGG ^CC[AT]GG HaeIII 205 GGCC GG^CC HapII 533 CCGG C^CGG HincII 349 GTCAAC GT[CT]^[GA]AC HindII 349 GTCAAC GT[CT]^[GA]AC HindIII 181 AAGCTT A^AGCTT HpaII 533 CCGG C^CGG HphI 227 TCACC GGTGA(8/7) MboII 81 TCTTC GAAGA(8/7) MfeI 128 CAATTG C^AATTG MspI 533 CCGG C^CGG MunI 128 CAATTG C^AATTG MvaI 207 CCAGG CC^[AT]GG NciI 532 CCCGG CC^[GC]GG NcoI 252 CCATGG C^CATGG NspV 218 TTCGAA TT^CGAA PpuMI 528 GGGTCCC [GA]G^G[AT]CC[CT] PstI 316 CTGCAG CTGCA^G RcaI 494 TCATGA T^CATGA Sau96I 529 GGTCC G^G[ACGT]CC SfaNI 257 GCATC GCATC(5/9) SfuI 218 TTCGAA TT^CGAA TaqI 219 TCGA T^CGA Tsp45I 277 GTGAC ^GT[GC]AC TthHB8I 219 TCGA T^CGA XmnI 211 GAAAGATTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC ################################### # TWO CUT ENZYMES ( 16 enzymes) # ################################### BmyI 248 GAGCCC G[AGT]GC[ACT]^C 579 GAGCAC BpmI 231 CTCCAG CTGGAG(16/14) 584 CTCCAG BsaJI 252 CCATGG C^C[ACGT][ACGT]GG 435 CCTTGG BsmFI 194 GGGAC GGGAC(10/14) 530 GTCCC Bsp1286I 248 GAGCCC G[AGT]GC[ACT]^C 579 GAGCAC BssKI 207 CCAGG ^CC[ACGT]GG 532 CCCGG EcoT14I 252 CCATGG C^C[AT][AT]GG 435 CCTTGG MaeIII 39 GTTAC ^GT[ACGT]AC 277 GTGAC MslI 261 CATAGTTGTG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG 495 CATGACGATG MspA1I 506 CAGCTG C[AC]G^C[GT]G 542 CAGCTG PleI 55 GACTC GAGTC(4/5) 289 GAGTC PvuII 506 CAGCTG CAG^CTG 542 CAGCTG ScrFI 207 CCAGG CC^[ACGT]GG 532 CCCGG SfcI 155 CTATAG C^T[GA][CT]AG 316 CTGCAG StyI 252 CCATGG C^C[AT][AT]GG 435 CCTTGG TfiI 12 GAATC G^A[AT]TC 430 GATTC ######################################### # 3 OR MORE CUT ENZYMES ( 22 enzymes) # ######################################### AcsI AluI ApoI BbvI BsrI DdeI DpnII Fnu4HI HinfI ItaI MboI MnlI MseI MwoI NdeII NlaIII NlaIV Sau3AI Tru9I TseI Tsp509I TspRI