Restriction map for
pFN21AB3778
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# NO CUT ENZYMES ( 174 enzymes) #
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AatII Acc65I AccI AccIII AclI
AfaI AflII AflIII AgeI Alw44I
AlwI AlwNI AocI Aor51HI ApaLI
AscI AseI AsnI Asp718I AspHI
AspI AvaII AviII AvrII BalI
BamHI BbeI BbrPI BbvCI BcgI
BciVI BclI BcnI BfaI BfrI
BglI BglII BlnI BlpI BpmI
Bpu1102I BsaAI BsaBI BsaI BsaJI
BseAI BsgI BsiEI BsiHKAI BsiWI
BsmAI BsmBI BsmFI BsmI Bsp1407I
BspDI BspEI BspHI BspLU11I BspMI
BsrBI BsrDI BsrFI BsrGI BssHII
BssSI Bst1107I BstAPI BstEII BstPI
BstXI BstYI BstZ17I Bsu36I CelII
Cfr10I ClaI CpoI DraI DraIII
DrdI DsaI EaeI EagI EclXI
Eco47III Eco52I Eco81I EcoNI EcoO65I
EcoRV EcoT14I EcoT22I FbaI FseI
FspI HaeII HincII HindII HindIII
HpaI HphI KasI KpnI KspI
MaeI MaeII MamI MfeI MflI
MluI MluNI MroI MscI MspA1I
MunI NaeI NarI NciI NcoI
NdeI NgoMIV NheI NotI NruI
NsiI NspI PacI PaeR7I PflFI
PflMI PinAI PmaCI PmeI PmlI
PpuMI PshAI PshBI Psp1406I PstI
PvuI PvuII RcaI RsaI RsrII
SacI SacII SalI SapI SbfI
ScaI SexAI SfiI SfoI SgrAI
SmaI SmlI SnaBI SpeI SphI
Sse8387I SspBI SspI StuI StyI
SwaI Tth111I Van91I XbaI XcmI
XhoI XhoII XmaCI XmaI
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# ONE CUT ENZYMES ( 38 enzymes) #
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AccII 5 CGCG CG^CG
AcyI 534 GACGCC G[GA]^CG[CT]C
AhdI 534 GACGCCAAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
ApaI 90 GGGCCC GGGCC^C
AspEI 534 GACGCCAAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
BanI 366 GGCACC G^G[CT][GA]CC
BanII 90 GGGCCC G[GA]GC[CT]^C
BmyI 90 GGGCCC G[AGT]GC[ACT]^C
BsaHI 534 GACGCC G[GA]^CG[CT]C
BsaWI 633 ACCGGA [AT]^CCGG[AT]
BseRI 355 GAGGAG GAGGAG(10/8)
Bsp1286I 90 GGGCCC G[AGT]GC[ACT]^C
BsrI 544 CCAGT ACTGG(1/-1)
BssKI 375 CCAGG ^CC[ACGT]GG
BstBI 444 TTCGAA TT^CGAA
BstNI 375 CCAGG CC^[AT]GG
BstUI 5 CGCG CG^CG
CfoI 267 GCGC GCG^C
DraII 90 GGGCCCT [GA]G^G[ACGT]CC[CT]
Eam1105I 534 GACGCCAAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EcoO109I 90 GGGCCCT [GA]G^G[ACGT]CC[CT]
EcoRI 156 GAATTC G^AATTC
EcoRII 375 CCAGG ^CC[AT]GG
FauI 642 CCCGC CCCGC(4/6)
HgaI 534 GACGC GACGC(5/10)
HhaI 267 GCGC GCG^C
Hin1I 534 GACGCC G[GA]^CG[CT]C
HinP1I 267 GCGC G^CGC
MseI 470 TTAA T^TAA
MslI 256 CATAAAAATG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
MvaI 375 CCAGG CC^[AT]GG
MvnI 5 CGCG CG^CG
NlaIII 321 CATG CATG^
NspV 444 TTCGAA TT^CGAA
ScrFI 375 CCAGG CC^[ACGT]GG
SfcI 527 CTACAG C^T[GA][CT]AG
SfuI 444 TTCGAA TT^CGAA
Tru9I 470 TTAA T^TAA
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# TWO CUT ENZYMES ( 26 enzymes) #
###################################
AcsI 107 AAATTC [GA]^AATT[CT]
156 GAATTC
ApoI 107 AAATTC [GA]^AATT[CT]
156 GAATTC
Asp700I 80 GAAGAGATTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
152 GAAAGAATTC
AvaI 122 CCCGAG C^[CT]CG[GA]G
676 CCCGAG
BbsI 184 GAAGAC GAAGAC(2/6)
198 GAAGAC
BbvI 295 GCAGC GCAGC(8/12)
392 GCAGC
BpuAI 184 GAAGAC GAAGAC(2/6)
198 GAAGAC
BsoBI 122 CCCGAG C^[CT]CG[GA]G
676 CCCGAG
Cac8I 279 GCAGGC GC[ACGT]^[ACGT]GC
641 GCCCGC
Cfr13I 90 GGGCC G^G[ACGT]CC
91 GGCCC
EarI 80 GAAGAG CTCTTC(1/4)
352 GAAGAG
HaeIII 8 GGCC GG^CC
91 GGCC
HapII 389 CCGG C^CGG
634 CCGG
HpaII 389 CCGG C^CGG
634 CCGG
Ksp632I 80 GAAGAG CTCTTC(1/4)
352 GAAGAG
MaeIII 431 GTGAC ^GT[ACGT]AC
532 GTGAC
MspI 389 CCGG C^CGG
634 CCGG
MwoI 286 GCATCTAAAGC GC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GC
580 GCTTCAAAGGC
PleI 178 GACTC GAGTC(4/5)
603 GACTC
Sau96I 90 GGGCC G^G[ACGT]CC
91 GGCCC
TaqI 111 TCGA T^CGA
445 TCGA
TseI 295 GCAGC G^C[AT]GC
392 GCAGC
Tsp45I 431 GTGAC ^GT[GC]AC
532 GTGAC
Tsp509I 108 AATT ^AATT
157 AATT
TthHB8I 111 TCGA T^CGA
445 TCGA
XmnI 80 GAAGAGATTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
152 GAAAGAATTC
#########################################
# 3 OR MORE CUT ENZYMES ( 20 enzymes) #
#########################################
AciI AluI BsiYI BslI BstF5I
DdeI DpnII Fnu4HI FokI HinfI
ItaI MboI MboII MnlI NdeII
NlaIV Sau3AI SfaNI TfiI TspRI