Restriction map for
pFN21AB6644
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# NO CUT ENZYMES ( 110 enzymes) #
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AatII Acc65I AccI AccIII AclI
AflII AflIII AhdI Alw44I AocI
Aor51HI ApaI ApaLI AscI AseI
AsnI Asp700I Asp718I AspEI AvrII
BbrPI BbvCI BclI BfrI BlnI
BlpI Bpu1102I BsaBI BseAI BsiEI
BsiWI BsmI Bsp1407I BspDI BspEI
BspHI BspLU11I BsrBI BsrDI BsrGI
Bst1107I BstAPI BstBI BstEII BstPI
BstXI BstZ17I Bsu36I CelII ClaI
CpoI DraI DraIII EagI Eam1105I
EclXI Eco47III Eco52I Eco81I EcoNI
EcoO65I EcoRI EcoT22I FbaI FseI
HincII HindII HpaI KpnI MamI
MfeI MluI MroI MseI MunI
NaeI NgoMIV NheI NotI NruI
NsiI NspV PacI PmaCI PmeI
PmlI PshAI PshBI Psp1406I PvuI
RcaI RsrII SacI SalI SbfI
ScaI SexAI SfiI SfuI SgrAI
SnaBI SpeI SphI Sse8387I SspBI
StuI SwaI Tru9I XcmI XmnI
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# ONE CUT ENZYMES ( 50 enzymes) #
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AcsI 757 AAATTT [GA]^AATT[CT]
AgeI 125 ACCGGT A^CCGGT
AlwNI 367 CAGCTGCTG CAG[ACGT][ACGT][ACGT]^CTG
ApoI 757 AAATTT [GA]^AATT[CT]
AspI 96 GACGCCGTC GAC[ACGT]^[ACGT][ACGT]GTC
AviII 635 TGCGCA TGC^GCA
BalI 1233 TGGCCA TGG^CCA
BamHI 148 GGATCC G^GATCC
BanII 600 GGGCTC G[GA]GC[CT]^C
BbeI 160 GGCGCC GGCGC^C
BcgI 248 CGACCCCGCTGC (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BglII 581 AGATCT A^GATCT
BpmI 1025 CTCCAG CTGGAG(16/14)
BsaAI 941 TACGTG [CT]AC^GT[GA]
BsaI 647 GAGACC GGTCTC(1/5)
BsmBI 101 CGTCTC CGTCTC(1/5)
BspMI 308 ACCTGC ACCTGC(4/8)
BssHII 25 GCGCGC G^CGCGC
BssSI 842 CTCGTG CACGAG(-5/-1)
DdeI 1179 CTCAG C^T[ACGT]AG
DrdI 1254 GACCAGGCCGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EcoRV 784 GATATC GAT^ATC
FspI 635 TGCGCA TGC^GCA
HindIII 1226 AAGCTT A^AGCTT
KasI 160 GGCGCC G^GCGCC
KspI 153 CCGCGG CCGC^GG
MluNI 1233 TGGCCA TGG^CCA
MscI 1233 TGGCCA TGG^CCA
MslI 1312 CACCCATATG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
NarI 160 GGCGCC GG^CGCC
NspI 681 GCATGT [GA]CATG^[CT]
PaeR7I 989 CTCGAG C^TCGAG
PflFI 96 GACGCCGTC GAC[ACGT]^[ACGT][ACGT]GTC
PflMI 735 CCATGCCATGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
PinAI 125 ACCGGT A^CCGGT
PpuMI 216 GGGTCCC [GA]G^G[AT]CC[CT]
PstI 559 CTGCAG CTGCA^G
PvuII 367 CAGCTG CAG^CTG
SacII 153 CCGCGG CCGC^GG
SapI 628 GAAGAGC GCTCTTC(1/4)
SfaNI 1052 GCATC GCATC(5/9)
SfcI 559 CTGCAG C^T[GA][CT]AG
SfoI 160 GGCGCC GGC^GCC
SmlI 989 CTCGAG C^T[CT][GA]AG
SspI 1184 AATATT AAT^ATT
Tsp45I 985 GTGAC ^GT[GC]AC
Tth111I 96 GACGCCGTC GAC[ACGT]^[ACGT][ACGT]GTC
Van91I 735 CCATGCCATGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
XbaI 804 TCTAGA T^CTAGA
XhoI 989 CTCGAG C^TCGAG
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# TWO CUT ENZYMES ( 24 enzymes) #
###################################
AcyI 96 GACGCC G[GA]^CG[CT]C
160 GGCGCC
BbsI 39 GTCTTC GAAGAC(2/6)
685 GTCTTC
BciVI 1061 GGATAC GTATCC(6/5)
498 GTATCC
BglI 47 GCCGTAGCGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
99 GCCGTCTCGGC
BpuAI 39 GTCTTC GAAGAC(2/6)
685 GTCTTC
BsaHI 96 GACGCC G[GA]^CG[CT]C
160 GGCGCC
BseRI 884 CTCCTC GAGGAG(10/8)
424 GAGGAG
BsgI 878 CTGCAC GTGCAG(16/14)
1082 CTGCAC
BsrFI 35 GCCGGT [GA]^CCGG[CT]
125 ACCGGT
Cfr10I 35 GCCGGT [GA]^CCGG[CT]
125 ACCGGT
DraII 216 GGGTCCC [GA]G^G[ACGT]CC[CT]
1271 AGGCCCC
EarI 565 GAAGAG CTCTTC(1/4)
628 GAAGAG
EcoO109I 216 GGGTCCC [GA]G^G[ACGT]CC[CT]
1271 AGGCCCC
Hin1I 96 GACGCC G[GA]^CG[CT]C
160 GGCGCC
HphI 123 TCACC GGTGA(8/7)
968 TCACC
Ksp632I 565 GAAGAG CTCTTC(1/4)
628 GAAGAG
MaeIII 985 GTGAC ^GT[ACGT]AC
1136 GTTAC
NcoI 59 CCATGG C^CATGG
740 CCATGG
NdeI 1094 CATATG CA^TATG
1316 CATATG
SmaI 131 CCCGGG CCC^GGG
164 CCCGGG
TaqI 341 TCGA T^CGA
990 TCGA
TthHB8I 341 TCGA T^CGA
990 TCGA
XmaCI 131 CCCGGG C^CCGGG
164 CCCGGG
XmaI 131 CCCGGG C^CCGGG
164 CCCGGG
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# 3 OR MORE CUT ENZYMES ( 74 enzymes) #
#########################################
AccII AciI AfaI AluI AlwI
AspHI AvaI AvaII BanI BbvI
BcnI BfaI BmyI BsaJI BsaWI
BsiHKAI BsiYI BslI BsmAI BsmFI
BsoBI Bsp1286I BsrI BssKI BstF5I
BstNI BstUI BstYI Cac8I CfoI
Cfr13I DpnII DsaI EaeI EcoRII
EcoT14I FauI Fnu4HI FokI HaeII
HaeIII HapII HgaI HhaI HinP1I
HinfI HpaII ItaI MaeI MaeII
MboI MboII MflI MnlI MspA1I
MspI MvaI MvnI MwoI NciI
NdeII NlaIII NlaIV PleI RsaI
Sau3AI Sau96I ScrFI StyI TfiI
TseI Tsp509I TspRI XhoII