(Circular) (Six-base) MAPSORT of: pFN21AB6644.seq Check: 5737 from: 1 to: 6034 pFN21AB6644 6034 bp With 182 enzymes: SgfI * August 16, 2010 14:51 .. AatII G_ACGT'C Cuts at: 278 331 414 600 1536 3844 278 Size: 53 83 186 936 2308 2468 Fragments arranged by size: 2468 2308 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 3377 1937 Size: 1440 4594 Acc65I G'GTAC_C Cuts at: 3355 3355 Size: 6034 AclI AA'CG_TT Cuts at: 1563 4161 4534 1563 Size: 2598 373 3063 Fragments arranged by size: 3063 2598 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 3293 4097 5481 5749 1632 Size: 1661 804 1384 268 1917 Fragments arranged by size: 1917 1661 1384 804 268 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 5837 AflIII A'CryG_T Cuts at: 1203 4933 5910 1203 Size: 3730 977 1327 Fragments arranged by size: 3730 1327 977 AgeI A'CCGG_T Cuts at: 2130 4865 2130 Size: 2735 3299 AhdI GACnn_n'nnGTC Cuts at: 4763 4763 Size: 6034 AleI CACnn'nnGTG Cuts at: 1166 1166 Size: 6034 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 1184 Size: 6034 AlwNI CAG_nnn'CTG Cuts at: 2377 3777 5349 2377 Size: 1400 1572 3062 Fragments arranged by size: 3062 1572 1400 ApaLI G'TGCA_C Cuts at: 4091 5247 4091 Size: 1156 4878 ApoI r'AATT_y Cuts at: 1493 2762 3343 3569 3721 5830 1493 Size: 1269 581 226 152 2109 1697 Fragments arranged by size: 2109 1697 1269 581 226 152 AseI AT'TA_AT Cuts at: 160 1032 4588 160 Size: 872 3556 1606 Fragments arranged by size: 3556 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 6034 AvaI C'yCGr_G Cuts at: 1870 1943 2136 2169 2931 2994 3359 1870 Size: 73 193 33 762 63 365 4545 Fragments arranged by size: 4545 762 365 193 73 63 33 BaeI ACnnnnGTAyCnnnnnnn_nnnnn' Cuts at: 2943 2976 2943 Size: 33 6001 BamHI G'GATC_C Cuts at: 2153 3364 3731 2153 Size: 1211 367 4456 Fragments arranged by size: 4456 1211 367 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2078 2165 2860 2981 3355 Size: 325 217 627 291 87 695 121 374 Cuts at: 3355 4810 618 Size: 1455 1842 Fragments arranged by size: 1842 1455 695 627 374 325 291 217 121 87 BanII G_rGCy'C Cuts at: 1391 2609 3353 1391 Size: 1218 744 4072 Fragments arranged by size: 4072 1218 744 BbeI G_GCGC'C Cuts at: 2169 2169 Size: 6034 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 2037 2683 928 Size: 971 138 646 4279 Fragments arranged by size: 4279 971 646 138 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 1987 2021 2242 2276 4225 4259 1903 Size: 34 50 34 221 34 1949 34 3678 Fragments arranged by size: 3678 1949 221 50 34 34 34 34 BciVI GTATCCnnnnn_n' Cuts at: 2514 3060 3926 5136 2514 Size: 546 866 1210 3412 Fragments arranged by size: 3412 1210 866 546 BclI T'GATC_A Cuts at: 1548 1548 Size: 6034 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 2058 2110 4645 Size: 107 122 71 631 605 386 52 2535 Cuts at: 4645 136 Size: 1525 Fragments arranged by size: 2535 1525 631 605 386 122 107 71 52 BglII A'GATC_T Cuts at: 2586 6029 2586 Size: 3443 2591 BlpI GC'TnA_GC Cuts at: 3451 3451 Size: 6034 Bme1580I G_kGCm'C Cuts at: 1790 2083 4095 5251 1790 Size: 293 2012 1156 2573 Fragments arranged by size: 2573 2012 1156 293 BmgBI CAC'GTC Cuts at: 1318 2855 1318 Size: 1537 4497 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 3765 4723 442 Size: 416 53 83 399 24 2348 958 1753 Fragments arranged by size: 2348 1753 958 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 6034 BplI GAGnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 2356 3045 2356 Size: 689 5345 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 3015 4694 1239 Size: 1776 1679 2579 Fragments arranged by size: 2579 1776 1679 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 4159 5024 5322 4159 Size: 865 298 4871 Fragments arranged by size: 4871 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 2646 4697 882 Size: 617 1147 2051 2219 Fragments arranged by size: 2219 2051 1147 617 BsaAI yAC'GTr Cuts at: 493 1204 2948 3825 493 Size: 711 1744 877 2702 Fragments arranged by size: 2702 1744 877 711 BsaBI GATnn'nnATC Cuts at: 6028 6028 Size: 6034 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 2102 2166 3841 4223 Size: 53 83 186 936 569 64 1675 382 Cuts at: 4223 4858 275 Size: 635 1451 Fragments arranged by size: 1675 1451 936 635 569 382 186 83 64 53 BsaWI w'CCGG_w Cuts at: 2075 2130 2773 4467 4865 5139 5286 5697 2075 Size: 55 643 1694 398 274 147 411 2412 Fragments arranged by size: 2412 1694 643 411 398 274 147 55 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 1178 Size: 30 165 30 5809 Fragments arranged by size: 5809 165 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2444 2880 1186 Size: 1258 436 4340 Fragments arranged by size: 4340 1258 436 BseYI C'CCAG_C Cuts at: 5237 5733 5237 Size: 496 5538 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 2868 3072 1163 Size: 573 1132 204 4125 Fragments arranged by size: 4125 1132 573 204 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 3484 4245 5273 1540 Size: 275 186 1483 761 1028 2301 Fragments arranged by size: 2301 1483 1028 761 275 186 BsiHKAI G_wGCw'C Cuts at: 3031 3046 3318 4095 4180 5251 3031 Size: 15 272 777 85 1071 3814 Fragments arranged by size: 3814 1071 777 272 85 15 BsmI GAATG_Cn' Cuts at: 3545 3638 3545 Size: 93 5941 BsmBI CGTCTCn'nnnn_ Cuts at: 2112 2112 Size: 6034 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2083 2609 3031 3046 3318 3353 4095 Size: 399 293 526 422 15 272 35 742 Cuts at: 4095 4180 5251 1391 Size: 85 1071 2174 Fragments arranged by size: 2174 1071 742 526 422 399 293 272 85 35 15 BspEI T'CCGG_A Cuts at: 5697 5697 Size: 6034 BspHI T'CATG_A Cuts at: 3923 5653 3923 Size: 1730 4304 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 2322 3389 844 Size: 1478 1067 3489 Fragments arranged by size: 3489 1478 1067 BsrBI CCG'CTC Cuts at: 1394 1823 3921 1394 Size: 429 2098 3507 Fragments arranged by size: 3507 2098 429 BsrDI GCAATG_nn' Cuts at: 65 1248 4529 4703 65 Size: 1183 3281 174 1396 Fragments arranged by size: 3281 1396 1183 174 BsrFI r'CCGG_y Cuts at: 1700 1810 2040 2130 4678 4865 1700 Size: 110 230 90 2548 187 2869 Fragments arranged by size: 2869 2548 230 187 110 90 BsrGI T'GTAC_A Cuts at: 96 96 Size: 6034 BssHII G'CGCG_C Cuts at: 1925 2030 1925 Size: 105 5929 BssSI C'ACGA_G Cuts at: 2847 4094 5106 2847 Size: 1247 1012 3775 Fragments arranged by size: 3775 1247 1012 BstAPI GCAn_nnn'nTGC Cuts at: 794 794 Size: 6034 BstBI TT'CG_AA Cuts at: 4838 4838 Size: 6034 BstEII G'GTnAC_C Cuts at: 1184 1184 Size: 6034 BstYI r'GATC_y Cuts at: 1972 2153 2586 2680 3364 3731 4116 4133 5585 Size: 181 433 94 684 367 385 17 1452 Cuts at: 5585 5671 5683 6029 1972 Size: 86 12 346 1977 Fragments arranged by size: 1977 1452 684 433 385 367 346 181 94 86 17 12 BtgI C'CryG_G Cuts at: 513 1065 2064 2158 2217 2745 2914 513 Size: 552 999 94 59 528 169 3633 Fragments arranged by size: 3633 999 552 528 169 94 59 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 487 Size: 683 56 5295 Fragments arranged by size: 5295 683 56 BtsI GCAGTG_nn' Cuts at: 3550 4344 4364 3550 Size: 794 20 5220 Fragments arranged by size: 5220 794 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 6034 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 5999 DraI TTT'AAA Cuts at: 3338 3338 Size: 6034 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 3265 5041 809 Size: 507 1949 1776 1802 Fragments arranged by size: 1949 1802 1776 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2214 2274 2328 Size: 54 1425 50 275 18 384 60 54 Cuts at: 2328 3238 3481 4370 5999 8 Size: 910 243 889 1629 43 Fragments arranged by size: 1629 1425 910 889 384 275 243 60 54 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 3481 1537 Size: 275 1669 4090 Fragments arranged by size: 4090 1669 275 EarI CTCTTCn'nnn_ Cuts at: 1353 2565 2628 3964 1353 Size: 1212 63 1336 3423 Fragments arranged by size: 3423 1336 1212 63 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 2160 4621 4995 5141 387 Size: 1064 138 571 2461 374 146 1280 Fragments arranged by size: 2461 1280 1064 571 374 146 138 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 3015 3293 4097 4694 5481 5749 1239 Size: 393 1383 278 804 597 787 268 1524 Fragments arranged by size: 1524 1383 804 787 597 393 278 268 EcoO109I rG'GnC_Cy Cuts at: 2222 3277 2222 Size: 1055 4979 EcoRI G'AATT_C Cuts at: 3343 3721 3343 Size: 378 5656 EcoRV GAT'ATC Cuts at: 2791 2791 Size: 6034 FspI TGC'GCA Cuts at: 2642 4540 2642 Size: 1898 4136 HaeII r_GCGC'y Cuts at: 1678 2014 2169 2252 5181 1678 Size: 336 155 83 2929 2531 Fragments arranged by size: 2929 2531 336 155 83 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2092 2124 4675 4707 4749 Size: 32 135 32 520 32 2551 32 42 Cuts at: 4749 4781 1373 Size: 32 2626 Fragments arranged by size: 2626 2551 520 135 42 32 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 3378 3624 669 Size: 988 281 1440 246 3079 Fragments arranged by size: 3079 1440 988 281 246 HindIII A'AGCT_T Cuts at: 748 3231 748 Size: 2483 3551 HpaI GTT'AAC Cuts at: 3624 3624 Size: 6034 KasI G'GCGC_C Cuts at: 2165 2165 Size: 6034 KpnI G_GTAC'C Cuts at: 3359 3359 Size: 6034 MfeI C'AATT_G Cuts at: 3633 3633 Size: 6034 MluI A'CGCG_T Cuts at: 5910 5910 Size: 6034 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 2902 5148 5332 1305 Size: 1597 2246 184 2007 Fragments arranged by size: 2246 2007 1597 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 3240 10 Size: 54 1425 343 1408 2804 Fragments arranged by size: 2804 1425 1408 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 3321 3992 4351 4510 518 Size: 648 2155 671 359 159 2042 Fragments arranged by size: 2155 2042 671 648 359 159 MspA1I CmG'CkG Cuts at: 1606 2122 2160 2200 2260 2374 3053 3450 4127 Size: 516 38 40 60 114 679 397 677 Cuts at: 4127 5275 5520 1606 Size: 1148 245 2120 Fragments arranged by size: 2120 1148 679 677 516 397 245 114 60 40 38 NarI GG'CG_CC Cuts at: 2166 2166 Size: 6034 NcoI C'CATG_G Cuts at: 513 1065 2064 2745 513 Size: 552 999 681 3802 Fragments arranged by size: 3802 999 681 552 NdeI CA'TA_TG Cuts at: 387 3100 3322 387 Size: 2713 222 3099 Fragments arranged by size: 3099 2713 222 NheI G'CTAG_C Cuts at: 1052 1052 Size: 6034 NotI GC'GGCC_GC Cuts at: 3481 3481 Size: 6034 NspI r_CATG'y Cuts at: 1132 1229 2690 3392 4937 1132 Size: 97 1461 702 1545 2229 Fragments arranged by size: 2229 1545 1461 702 97 PasI CC'CwG_GG Cuts at: 1348 2907 1348 Size: 1559 4475 PciI A'CATG_T Cuts at: 4933 4933 Size: 6034 PflMI CCAn_nnn'nTGG Cuts at: 1108 2746 1108 Size: 1638 4396 PmeI GTTT'AAAC Cuts at: 3338 3338 Size: 6034 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 4103 4135 5616 5648 4103 Size: 32 1481 32 4489 Fragments arranged by size: 4489 1481 32 32 PpuMI rG'GwC_Cy Cuts at: 2222 2222 Size: 6034 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 6034 PsiI TTA'TAA Cuts at: 3604 3604 Size: 6034 PspXI vC'TCGA_Gb Cuts at: 2994 2994 Size: 6034 PsrI GAACnnnnnnTACnnnnnnn_nnnnn' Cuts at: 2495 2527 2495 Size: 32 6002 PstI C_TGCA'G Cuts at: 830 2568 3386 830 Size: 1738 818 3478 Fragments arranged by size: 3478 1738 818 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 6034 PvuII CAG'CTG Cuts at: 2374 2374 Size: 6034 SacII CC_GC'GG Cuts at: 2161 2161 Size: 6034 SalI G'TCGA_C Cuts at: 1936 3376 1936 Size: 1440 4594 SanDI GG'GwC_CC Cuts at: 2222 2222 Size: 6034 SapI GCTCTTCn'nnn_ Cuts at: 2628 2628 Size: 6034 SbfI CC_TGCA'GG Cuts at: 3386 3386 Size: 6034 ScaI AGT'ACT Cuts at: 4282 5994 4282 Size: 1712 4322 SfcI C'TryA_G Cuts at: 826 1045 2564 3382 4517 5198 5389 826 Size: 219 1519 818 1135 681 191 1471 Fragments arranged by size: 1519 1471 1135 818 681 219 191 SfoI GGC'GCC Cuts at: 2167 2167 Size: 6034 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 6034 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 6034 SmaI CCC'GGG Cuts at: 1872 2138 2171 3361 1872 Size: 266 33 1190 4545 Fragments arranged by size: 4545 1190 266 33 SmlI C'TyrA_G Cuts at: 820 1017 1943 2994 4138 5039 5301 820 Size: 197 926 1051 1144 901 262 1553 Fragments arranged by size: 1553 1144 1051 926 901 262 197 SnaBI TAC'GTA Cuts at: 493 3825 493 Size: 3332 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 6034 SphI G_CATG'C Cuts at: 1132 3392 1132 Size: 2260 3774 SrfI GCCC'GGGC Cuts at: 2171 2171 Size: 6034 SspI AAT'ATT Cuts at: 5 52 3191 3958 5 Size: 47 3139 767 2081 Fragments arranged by size: 3139 2081 767 47 StuI AGG'CCT Cuts at: 1517 1517 Size: 6034 StyI C'CwwG_G Cuts at: 513 1065 2064 2625 2745 3473 513 Size: 552 999 561 120 728 3074 Fragments arranged by size: 3074 999 728 561 552 120 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 2275 3333 4061 4078 4231 4416 4861 Size: 1255 695 1058 728 17 153 185 445 Cuts at: 4861 325 Size: 1498 Fragments arranged by size: 1498 1255 1058 728 695 445 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 4280 5992 96 Size: 274 80 33 51 1443 2303 1712 138 Fragments arranged by size: 2303 1712 1443 274 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 2844 2876 5994 6026 2844 Size: 32 3118 32 2852 Fragments arranged by size: 3118 2852 32 32 Tth111I GACn'n_nGTC Cuts at: 1316 2104 1316 Size: 788 5246 XbaI T'CTAG_A Cuts at: 2809 3370 2809 Size: 561 5473 XhoI C'TCGA_G Cuts at: 1943 2994 1943 Size: 1051 4983 XmaI C'CCGG_G Cuts at: 1870 2136 2169 3359 1870 Size: 266 33 1190 4545 Fragments arranged by size: 4545 1190 266 33 XmnI GAAnn'nnTTC Cuts at: 4163 4163 Size: 6034 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 3842 276 Size: 53 83 186 936 2308 2468 Fragments arranged by size: 2468 2308 936 186 83 53 Enzymes that do cut: AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI AvaI BaeI BamHI BanI BanII BbeI BbsI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BplI BpmI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI BsmBI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstYI BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI EarI EciI Eco57MI EcoO109I EcoRI EcoRV FspI HaeII Hin4I HincII HindIII HpaI KasI KpnI MfeI MluI MmeI MscI MslI MspA1I NarI NcoI NdeI NheI NotI NspI PasI PciI PflMI PmeI PpiI PpuMI PshAI PsiI PspXI PsrI PstI PvuI PvuII SacII SalI SanDI SapI SbfI ScaI SfcI SfoI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SrfI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XhoI XmaI XmnI ZraI Enzymes that do not cut: AarI AfeI AloI ApaI AscI AvrII BbvCI BfrBI Bpu10I BsiWI BstXI BstZ17I Bsu36I DraIII EcoICRI EcoNI FalI FseI FspAI NaeI NgoMIV NruI NsiI PacI PfoI PmlI PspOMI RsrII SacI SexAI SfiI SwaI XcmI