(Linear) (Six-base) MAPSORT of: KIBB6644.seq Check: 9772 from: 1 to: 1329 KIBB6644.orf With 182 enzymes: SgfI * July 15, 2010 18:30 .. AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 0 1288 1329 Size: 1288 41 AgeI A'CCGG_T Cuts at: 0 125 1329 Size: 125 1204 AlwNI CAG_nnn'CTG Cuts at: 0 372 1329 Size: 372 957 ApoI r'AATT_y Cuts at: 0 757 1329 Size: 757 572 AvaI C'yCGr_G Cuts at: 0 131 164 926 989 1329 Size: 131 33 762 63 340 Fragments arranged by size: 762 340 131 63 33 BaeI ACnnnnGTAyCnnnnnnn_nnnnn' Cuts at: 0 938 971 1329 Size: 938 33 358 Fragments arranged by size: 938 358 33 BamHI G'GATC_C Cuts at: 0 148 1329 Size: 148 1181 BanI G'GyrC_C Cuts at: 0 73 160 855 976 1329 Size: 73 87 695 121 353 Fragments arranged by size: 695 353 121 87 73 BanII G_rGCy'C Cuts at: 0 604 1329 Size: 604 725 BbeI G_GCGC'C Cuts at: 0 164 1329 Size: 164 1165 BbsI GAAGACnn'nnnn_ Cuts at: 0 32 678 1329 Size: 32 646 651 Fragments arranged by size: 651 646 32 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 0 237 271 1329 Size: 237 34 1058 Fragments arranged by size: 1058 237 34 BciVI GTATCCnnnnn_n' Cuts at: 0 509 1055 1329 Size: 509 546 274 Fragments arranged by size: 546 509 274 BglI GCCn_nnn'nGGC Cuts at: 0 53 105 1329 Size: 53 52 1224 Fragments arranged by size: 1224 53 52 BglII A'GATC_T Cuts at: 0 581 1329 Size: 581 748 Bme1580I G_kGCm'C Cuts at: 0 78 1329 Size: 78 1251 BmgBI CAC'GTC Cuts at: 0 850 1329 Size: 850 479 BplI GAGnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 0 351 1040 1329 Size: 351 689 289 Fragments arranged by size: 689 351 289 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 0 1010 1329 Size: 1010 319 BsaI GGTCTCn'nnnn_ Cuts at: 0 641 1329 Size: 641 688 BsaAI yAC'GTr Cuts at: 0 943 1329 Size: 943 386 BsaHI Gr'CG_yC Cuts at: 0 97 161 1329 Size: 97 64 1168 Fragments arranged by size: 1168 97 64 BsaWI w'CCGG_w Cuts at: 0 70 125 768 1329 Size: 70 55 643 561 Fragments arranged by size: 643 561 70 55 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 0 439 875 1329 Size: 439 436 454 Fragments arranged by size: 454 439 436 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 0 863 1067 1329 Size: 863 204 262 Fragments arranged by size: 863 262 204 BsiHKAI G_wGCw'C Cuts at: 0 1026 1041 1313 1329 Size: 1026 15 272 16 Fragments arranged by size: 1026 272 16 15 BsmBI CGTCTCn'nnnn_ Cuts at: 0 107 1329 Size: 107 1222 Bsp1286I G_dGCh'C Cuts at: 0 78 604 1026 1041 1313 1329 Size: 78 526 422 15 272 16 Fragments arranged by size: 526 422 272 78 16 15 BspMI ACCTGCnnnn'nnnn_ Cuts at: 0 317 1329 Size: 317 1012 BsrFI r'CCGG_y Cuts at: 0 35 125 1329 Size: 35 90 1204 Fragments arranged by size: 1204 90 35 BssHII G'CGCG_C Cuts at: 0 25 1329 Size: 25 1304 BssSI C'ACGA_G Cuts at: 0 842 1329 Size: 842 487 BstYI r'GATC_y Cuts at: 0 148 581 675 1329 Size: 148 433 94 654 Fragments arranged by size: 654 433 148 94 BtgI C'CryG_G Cuts at: 0 59 153 212 740 909 1329 Size: 59 94 59 528 169 420 Fragments arranged by size: 528 420 169 94 59 59 DrdI GACnn_nn'nnGTC Cuts at: 0 1260 1329 Size: 1260 69 EaeI y'GGCC_r Cuts at: 0 209 269 323 1233 1329 Size: 209 60 54 910 96 Fragments arranged by size: 910 209 96 60 54 EarI CTCTTCn'nnn_ Cuts at: 0 560 623 1329 Size: 560 63 706 Fragments arranged by size: 706 560 63 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 0 155 1329 Size: 155 1174 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 0 1010 1288 1329 Size: 1010 278 41 Fragments arranged by size: 1010 278 41 EcoO109I rG'GnC_Cy Cuts at: 0 217 1272 1329 Size: 217 1055 57 Fragments arranged by size: 1055 217 57 EcoRV GAT'ATC Cuts at: 0 786 1329 Size: 786 543 FspI TGC'GCA Cuts at: 0 637 1329 Size: 637 692 HaeII r_GCGC'y Cuts at: 0 9 164 247 1329 Size: 9 155 83 1082 Fragments arranged by size: 1082 155 83 9 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 0 87 119 1329 Size: 87 32 1210 Fragments arranged by size: 1210 87 32 HindIII A'AGCT_T Cuts at: 0 1226 1329 Size: 1226 103 KasI G'GCGC_C Cuts at: 0 160 1329 Size: 160 1169 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 0 897 1329 Size: 897 432 MscI TGG'CCA Cuts at: 0 1235 1329 Size: 1235 94 MslI CAynn'nnrTG Cuts at: 0 1316 1329 Size: 1316 13 MspA1I CmG'CkG Cuts at: 0 117 155 195 255 369 1048 1329 Size: 117 38 40 60 114 679 281 Fragments arranged by size: 679 281 117 114 60 40 38 NarI GG'CG_CC Cuts at: 0 161 1329 Size: 161 1168 NcoI C'CATG_G Cuts at: 0 59 740 1329 Size: 59 681 589 Fragments arranged by size: 681 589 59 NdeI CA'TA_TG Cuts at: 0 1095 1317 1329 Size: 1095 222 12 Fragments arranged by size: 1095 222 12 NspI r_CATG'y Cuts at: 0 685 1329 Size: 685 644 PasI CC'CwG_GG Cuts at: 0 902 1329 Size: 902 427 PflMI CCAn_nnn'nTGG Cuts at: 0 741 1329 Size: 741 588 PpuMI rG'GwC_Cy Cuts at: 0 217 1329 Size: 217 1112 PspXI vC'TCGA_Gb Cuts at: 0 989 1329 Size: 989 340 PsrI GAACnnnnnnTACnnnnnnn_nnnnn' Cuts at: 0 490 522 1329 Size: 490 32 807 Fragments arranged by size: 807 490 32 PstI C_TGCA'G Cuts at: 0 563 1329 Size: 563 766 PvuII CAG'CTG Cuts at: 0 369 1329 Size: 369 960 SacII CC_GC'GG Cuts at: 0 156 1329 Size: 156 1173 SanDI GG'GwC_CC Cuts at: 0 217 1329 Size: 217 1112 SapI GCTCTTCn'nnn_ Cuts at: 0 623 1329 Size: 623 706 SfcI C'TryA_G Cuts at: 0 559 1329 Size: 559 770 SfoI GGC'GCC Cuts at: 0 162 1329 Size: 162 1167 SmaI CCC'GGG Cuts at: 0 133 166 1329 Size: 133 33 1163 Fragments arranged by size: 1163 133 33 SmlI C'TyrA_G Cuts at: 0 989 1329 Size: 989 340 SrfI GCCC'GGGC Cuts at: 0 166 1329 Size: 166 1163 SspI AAT'ATT Cuts at: 0 1186 1329 Size: 1186 143 StyI C'CwwG_G Cuts at: 0 59 620 740 1329 Size: 59 561 120 589 Fragments arranged by size: 589 561 120 59 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 0 270 1328 1329 Size: 270 1058 1 Fragments arranged by size: 1058 270 1 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 0 839 871 1329 Size: 839 32 458 Fragments arranged by size: 839 458 32 Tth111I GACn'n_nGTC Cuts at: 0 99 1329 Size: 99 1230 XbaI T'CTAG_A Cuts at: 0 804 1329 Size: 804 525 XhoI C'TCGA_G Cuts at: 0 989 1329 Size: 989 340 XmaI C'CCGG_G Cuts at: 0 131 164 1329 Size: 131 33 1165 Fragments arranged by size: 1165 131 33 Enzymes that do cut: AcuI AgeI AlwNI ApoI AvaI BaeI BamHI BanI BanII BbeI BbsI BcgI BciVI BglI BglII Bme1580I BmgBI BplI BpmI BsaI BsaAI BsaHI BsaWI BseRI BsgI BsiHKAI BsmBI Bsp1286I BspMI BsrFI BssHII BssSI BstYI BtgI DrdI EaeI EarI EciI Eco57MI EcoO109I EcoRV FspI HaeII Hin4I HindIII KasI MmeI MscI MslI MspA1I NarI NcoI NdeI NspI PasI PflMI PpuMI PspXI PsrI PstI PvuII SacII SanDI SapI SfcI SfoI SmaI SmlI SrfI SspI StyI TaqII TstI Tth111I XbaI XhoI XmaI Enzymes that do not cut: AarI AatII AccI Acc65I AclI AfeI AflII AflIII AhdI AleI AlfI AloI ApaI ApaLI AscI AseI AsiSI AvrII BbvCI BclI BfrBI BlpI BmrI BmtI Bpu10I BpuEI BsaBI BsaXI BseYI BsiEI BsiWI BsmI BspEI BspHI BsrBI BsrDI BsrGI BstAPI BstBI BstEII BstXI BstZ17I Bsu36I BtgZI BtsI ClaI CspCI DraI DraIII EagI EcoICRI EcoNI EcoRI FalI FseI FspAI HincII HpaI KpnI MfeI MluI NaeI NgoMIV NheI NotI NruI NsiI PacI PciI PfoI PmeI PmlI PpiI PshAI PsiI PspOMI PvuI RsrII SacI SalI SbfI ScaI SexAI SfiI SgfI SgrAI SnaBI SpeI SphI StuI SwaI TatI XcmI XmnI ZraI