################################## # NO CUT ENZYMES ( 133 enzymes) # ################################## AatII Acc65I AccI AccIII AclI AflII AgeI AhdI Alw44I AlwNI AocI Aor51HI ApaI ApaLI AscI AseI AsnI Asp700I Asp718I AspEI AspHI AviII AvrII BamHI BbrPI BclI BfaI BfrI BglI BglII BlnI BlpI Bpu1102I BsaAI BsaBI BseAI BsiEI BsiHKAI BsiWI BsmI BspDI BspEI BspHI BspLU11I BspMI BsrBI BssHII BssSI Bst1107I BstAPI BstBI BstEII BstPI BstZ17I Bsu36I CelII ClaI CpoI DraI DraII DraIII DrdI EagI Eam1105I EclXI Eco47III Eco52I Eco81I EcoO109I EcoO65I EcoRI EcoRV EcoT14I FbaI FseI FspI HincII HindII HindIII HpaI KpnI KspI MaeI MamI MfeI MroI MspA1I MunI NcoI NdeI NheI NotI NruI NspV PacI PaeR7I PflMI PinAI PleI PmaCI PmeI PmlI PpuMI PshAI PshBI Psp1406I PvuI PvuII RcaI RsrII SacI SacII SalI SapI SbfI ScaI SfiI SfuI SgrAI SmaI SmlI SnaBI SpeI Sse8387I StyI SwaI Van91I XbaI XcmI XhoI XmaCI XmaI XmnI ################################### # ONE CUT ENZYMES ( 60 enzymes) # ################################### AcsI 324 AAATTT [GA]^AATT[CT] AfaI 438 GTAC GT^AC AflIII 713 ACGCGT A^C[GA][CT]GT ApoI 324 AAATTT [GA]^AATT[CT] AspI 379 GACTATGTC GAC[ACGT]^[ACGT][ACGT]GTC AvaI 860 CCCGAG C^[CT]CG[GA]G AvaII 491 GGTCC G^G[AT]CC BalI 398 TGGCCA TGG^CCA BanII 64 GAGCCC G[GA]GC[CT]^C BbeI 39 GGCGCC GGCGC^C BbvCI 901 GCTGAGG CCTCAGC(-5/-2) BciVI 839 GTATCC GTATCC(6/5) BmyI 64 GAGCCC G[AGT]GC[ACT]^C BsaI 585 GGTCTC GGTCTC(1/5) BsaWI 776 ACCGGA [AT]^CCGG[AT] BsmBI 121 CGTCTC CGTCTC(1/5) BsoBI 860 CCCGAG C^[CT]CG[GA]G Bsp1286I 64 GAGCCC G[AGT]GC[ACT]^C Bsp1407I 437 TGTACA T^GTACA BsrGI 437 TGTACA T^GTACA BsrI 81 ACTGG ACTGG(1/-1) BstF5I 579 GGATG GGATG(2/0) BstXI 68 CCATCAGCCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG BstYI 189 GGATCT [GA]^GATC[CT] CfoI 40 GCGC GCG^C DsaI 47 CCGTGG C^C[GA][CT]GG EarI 133 CTCTTC CTCTTC(1/4) EcoNI 482 CCTGTGCGAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG EcoT22I 523 ATGCAT ATGCA^T FokI 579 GGATG GGATG(9/13) HaeII 39 GGCGCC [GA]GCGC^[CT] HhaI 40 GCGC GCG^C HinP1I 40 GCGC G^CGC HinfI 462 GATTC G^A[ACGT]TC KasI 39 GGCGCC G^GCGCC Ksp632I 133 CTCTTC CTCTTC(1/4) MaeII 473 ACGT A^CGT MflI 189 GGATCT [GA]^GATC[CT] MluI 713 ACGCGT A^CGCGT MluNI 398 TGGCCA TGG^CCA MscI 398 TGGCCA TGG^CCA MslI 642 CACAGATGTG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG NaeI 91 GCCGGC GCC^GGC NarI 39 GGCGCC GG^CGCC NgoMIV 91 GCCGGC G^CCGGC NsiI 523 ATGCAT ATGCA^T PflFI 379 GACTATGTC GAC[ACGT]^[ACGT][ACGT]GTC PstI 181 CTGCAG CTGCA^G RsaI 438 GTAC GT^AC SexAI 572 ACCTGGT A^CC[AT]GGT SfcI 181 CTGCAG C^T[GA][CT]AG SfoI 39 GGCGCC GGC^GCC SphI 525 GCATGC GCATG^C SspBI 437 TGTACA T^GTACA SspI 352 AATATT AAT^ATT StuI 531 AGGCCT AGG^CCT TfiI 462 GATTC G^A[AT]TC Tsp45I 377 GTGAC ^GT[GC]AC Tth111I 379 GACTATGTC GAC[ACGT]^[ACGT][ACGT]GTC XhoII 189 GGATCT [GA]^GATC[CT] ################################### # TWO CUT ENZYMES ( 26 enzymes) # ################################### AccII 119 CGCG CG^CG 714 CGCG AcyI 39 GGCGCC G[GA]^CG[CT]C 94 GGCGTC BanI 39 GGCGCC G^G[CT][GA]CC 313 GGCACC BbsI 685 GAAGAC GAAGAC(2/6) 880 GAAGAC BcgI 320 GCAAAAATTTCG (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10) 335 GCAAGATCATCG BcnI 12 CCGGG CC^[GC]GG 171 CCGGG BpmI 626 CTGGAG CTGGAG(16/14) 663 CTGGAG BpuAI 685 GAAGAC GAAGAC(2/6) 880 GAAGAC BsaHI 39 GGCGCC G[GA]^CG[CT]C 94 GGCGTC BsgI 298 CTGCAC GTGCAG(16/14) 52 GTGCAG BsmAI 122 GTCTC GTCTC(1/5) 586 GTCTC BsmFI 78 GGGAC GGGAC(10/14) 858 GTCCC BsrDI 404 CATTGC GCAATG(2/0) 620 GCAATG BstUI 119 CGCG CG^CG 714 CGCG EaeI 89 TGGCCG [CT]^GGCC[GA] 398 TGGCCA FauI 117 CCCGC CCCGC(4/6) 149 GCGGG HgaI 95 GCGTC GACGC(5/10) 120 GCGTC Hin1I 39 GGCGCC G[GA]^CG[CT]C 94 GGCGTC MaeIII 377 GTGAC ^GT[ACGT]AC 450 GTTAC MseI 238 TTAA T^TAA 769 TTAA MvnI 119 CGCG CG^CG 714 CGCG NciI 12 CCGGG CC^[GC]GG 171 CCGGG NspI 417 ACATGC [GA]CATG^[CT] 525 GCATGC TaqI 465 TCGA T^CGA 560 TCGA Tru9I 238 TTAA T^TAA 769 TTAA TthHB8I 465 TCGA T^CGA 560 TCGA ######################################### # 3 OR MORE CUT ENZYMES ( 39 enzymes) # ######################################### AciI AluI AlwI BbvI BsaJI BseRI BsiYI BslI BsrFI BssKI BstNI Cac8I Cfr10I Cfr13I DdeI DpnII EcoRII Fnu4HI HaeIII HapII HpaII HphI ItaI MboI MboII MnlI MspI MvaI MwoI NdeII NlaIII NlaIV Sau3AI Sau96I ScrFI SfaNI TseI Tsp509I TspRI