(Circular) (Six-base) MAPSORT of: pFN21AE1352.seq Check: 6065 from: 1 to: 5701 pFN21AE1352 5701 bp With 182 enzymes: SgfI * August 16, 2010 14:45 .. AatII G_ACGT'C Cuts at: 278 331 414 600 1536 3511 278 Size: 53 83 186 936 1975 2468 Fragments arranged by size: 2468 1975 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 3044 1937 Size: 1107 4594 Acc65I G'GTAC_C Cuts at: 3022 3022 Size: 5701 AclI AA'CG_TT Cuts at: 1563 3828 4201 1563 Size: 2265 373 3063 Fragments arranged by size: 3063 2265 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 3764 5148 5416 1632 Size: 2132 1384 268 1917 Fragments arranged by size: 2132 1917 1384 268 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 5504 AflIII A'CryG_T Cuts at: 1203 2718 4600 5577 1203 Size: 1515 1882 977 1327 Fragments arranged by size: 1882 1515 1327 977 AgeI A'CCGG_T Cuts at: 4532 4532 Size: 5701 AhdI GACnn_n'nnGTC Cuts at: 4430 4430 Size: 5701 AleI CACnn'nnGTG Cuts at: 1166 2651 1166 Size: 1485 4216 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 1184 Size: 5701 AlwNI CAG_nnn'CTG Cuts at: 3444 5016 3444 Size: 1572 4129 ApaLI G'TGCA_C Cuts at: 3758 4914 3758 Size: 1156 4545 ApoI r'AATT_y Cuts at: 1493 2329 3010 3236 3388 5497 1493 Size: 836 681 226 152 2109 1697 Fragments arranged by size: 2109 1697 836 681 226 152 AseI AT'TA_AT Cuts at: 160 1032 4255 160 Size: 872 3223 1606 Fragments arranged by size: 3223 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 5701 AvaI C'yCGr_G Cuts at: 1870 1943 2865 3026 1870 Size: 73 922 161 4545 Fragments arranged by size: 4545 922 161 73 BamHI G'GATC_C Cuts at: 3031 3398 3031 Size: 367 5334 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2044 2318 3022 4477 618 Size: 325 217 627 257 274 704 1455 1842 Fragments arranged by size: 1842 1455 704 627 325 274 257 217 BanII G_rGCy'C Cuts at: 1391 2073 3020 1391 Size: 682 947 4072 Fragments arranged by size: 4072 947 682 BbeI G_GCGC'C Cuts at: 2048 2048 Size: 5701 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 2697 2892 928 Size: 971 798 195 3737 Fragments arranged by size: 3737 971 798 195 BbvCI CC'TCA_GC Cuts at: 2907 2907 Size: 5701 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 2314 2329 2348 2363 3892 3926 1903 Size: 34 377 15 19 15 1529 34 3678 Fragments arranged by size: 3678 1529 377 34 34 19 15 15 BciVI GTATCCnnnnn_n' Cuts at: 2855 3593 4803 2855 Size: 738 1210 3753 Fragments arranged by size: 3753 1210 738 BclI T'GATC_A Cuts at: 1548 1548 Size: 5701 BfrBI ATG'CAT Cuts at: 2530 2530 Size: 5701 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 4312 136 Size: 107 122 71 631 605 2640 1525 Fragments arranged by size: 2640 1525 631 605 122 107 71 BglII A'GATC_T Cuts at: 5696 5696 Size: 5701 BlpI GC'TnA_GC Cuts at: 3118 3118 Size: 5701 Bme1580I G_kGCm'C Cuts at: 1790 3762 4918 1790 Size: 1972 1156 2573 Fragments arranged by size: 2573 1972 1156 BmgBI CAC'GTC Cuts at: 1318 1318 Size: 5701 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 3432 4390 442 Size: 416 53 83 399 24 2015 958 1753 Fragments arranged by size: 2015 1753 958 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 5701 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2652 2689 4361 1239 Size: 1413 37 1672 2579 Fragments arranged by size: 2579 1672 1413 37 Bpu10I CC'TnA_GC Cuts at: 2171 2907 2171 Size: 736 4965 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 3826 4691 4989 3826 Size: 865 298 4538 Fragments arranged by size: 4538 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 2596 4364 882 Size: 617 1097 1768 2219 Fragments arranged by size: 2219 1768 1097 617 BsaAI yAC'GTr Cuts at: 493 1204 3492 493 Size: 711 2288 2702 Fragments arranged by size: 2702 2288 711 BsaBI GATnn'nnATC Cuts at: 5695 5695 Size: 5701 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 2045 2100 3508 3890 Size: 53 83 186 936 512 55 1408 382 Cuts at: 3890 4525 275 Size: 635 1451 Fragments arranged by size: 1451 1408 936 635 512 382 186 83 55 53 BsaWI w'CCGG_w Cuts at: 2781 4134 4532 4806 4953 5364 2781 Size: 1353 398 274 147 411 3118 Fragments arranged by size: 3118 1353 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 1178 Size: 30 165 30 5476 Fragments arranged by size: 5476 165 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2686 2924 2948 1186 Size: 1500 238 24 3939 Fragments arranged by size: 3939 1500 238 24 BseYI C'CCAG_C Cuts at: 2011 2026 4904 5400 2011 Size: 15 2878 496 2312 Fragments arranged by size: 2878 2312 496 15 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 2078 2288 1163 Size: 573 342 210 4576 Fragments arranged by size: 4576 573 342 210 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 3151 3912 4940 1540 Size: 275 186 1150 761 1028 2301 Fragments arranged by size: 2301 1150 1028 761 275 186 BsiHKAI G_wGCw'C Cuts at: 3762 3847 4918 3762 Size: 85 1071 4545 Fragments arranged by size: 4545 1071 85 BsmI GAATG_Cn' Cuts at: 3212 3305 3212 Size: 93 5608 BsmBI CGTCTCn'nnnn_ Cuts at: 2132 2132 Size: 5701 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2073 3020 3762 3847 4918 1391 Size: 399 283 947 742 85 1071 2174 Fragments arranged by size: 2174 1071 947 742 399 283 85 BspEI T'CCGG_A Cuts at: 5364 5364 Size: 5701 BspHI T'CATG_A Cuts at: 3590 5320 3590 Size: 1730 3971 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 3056 844 Size: 2212 3489 BsrBI CCG'CTC Cuts at: 1394 1823 3588 1394 Size: 429 1765 3507 Fragments arranged by size: 3507 1765 429 BsrDI GCAATG_nn' Cuts at: 65 1248 2408 2632 4196 4370 65 Size: 1183 1160 224 1564 174 1396 Fragments arranged by size: 1564 1396 1183 1160 224 174 BsrFI r'CCGG_y Cuts at: 1700 1810 2096 2108 2321 4345 4532 1700 Size: 110 286 12 213 2024 187 2869 Fragments arranged by size: 2869 2024 286 213 187 110 12 BsrGI T'GTAC_A Cuts at: 96 2442 96 Size: 2346 3355 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 5701 BssSI C'ACGA_G Cuts at: 3761 4773 3761 Size: 1012 4689 BstAPI GCAn_nnn'nTGC Cuts at: 794 794 Size: 5701 BstBI TT'CG_AA Cuts at: 4505 4505 Size: 5701 BstEII G'GTnAC_C Cuts at: 1184 1184 Size: 5701 BstXI CCAn_nnnn'nTGG Cuts at: 2011 2080 2011 Size: 69 5632 BstYI r'GATC_y Cuts at: 1972 2194 3031 3398 3783 3800 5252 5338 5350 Size: 222 837 367 385 17 1452 86 12 Cuts at: 5350 5696 1972 Size: 346 1977 Fragments arranged by size: 1977 1452 837 385 367 346 222 86 17 12 BtgI C'CryG_G Cuts at: 513 1065 2052 513 Size: 552 987 4162 Fragments arranged by size: 4162 987 552 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 2332 487 Size: 683 56 1106 3856 Fragments arranged by size: 3856 1106 683 56 BtsI GCAGTG_nn' Cuts at: 2183 3217 4011 4031 2183 Size: 1034 794 20 3853 Fragments arranged by size: 3853 1034 794 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 5701 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 2734 2769 529 Size: 35 2170 35 3461 Fragments arranged by size: 3461 2170 35 35 DraI TTT'AAA Cuts at: 3005 3005 Size: 5701 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 4708 809 Size: 507 3392 1802 Fragments arranged by size: 3392 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2094 2403 3148 Size: 54 1425 50 275 18 264 309 745 Cuts at: 3148 4037 5666 8 Size: 889 1629 43 Fragments arranged by size: 1629 1425 889 745 309 275 264 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 3148 1537 Size: 275 1336 4090 Fragments arranged by size: 4090 1336 275 EarI CTCTTCn'nnn_ Cuts at: 1353 2144 3631 1353 Size: 791 1487 3423 Fragments arranged by size: 3423 1487 791 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 4288 4662 4808 387 Size: 1064 138 2699 374 146 1280 Fragments arranged by size: 2699 1280 1064 374 146 138 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2652 2689 3764 4361 5148 5416 1239 Size: 393 1020 37 1075 597 787 268 1524 Fragments arranged by size: 1524 1075 1020 787 597 393 268 37 EcoNI CCTnn'n_nnAGG Cuts at: 2491 2491 Size: 5701 EcoRI G'AATT_C Cuts at: 3010 3388 3010 Size: 378 5323 FspI TGC'GCA Cuts at: 4207 4207 Size: 5701 HaeII r_GCGC'y Cuts at: 1678 2048 4848 1678 Size: 370 2800 2531 Fragments arranged by size: 2800 2531 370 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2576 2608 4342 4374 4416 Size: 32 135 32 1004 32 1734 32 42 Cuts at: 4416 4448 1373 Size: 32 2626 Fragments arranged by size: 2626 1734 1004 135 42 32 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 3045 3291 669 Size: 988 281 1107 246 3079 Fragments arranged by size: 3079 1107 988 281 246 HindIII A'AGCT_T Cuts at: 748 748 Size: 5701 HpaI GTT'AAC Cuts at: 3291 3291 Size: 5701 KasI G'GCGC_C Cuts at: 2044 2044 Size: 5701 KpnI G_GTAC'C Cuts at: 3026 3026 Size: 5701 MfeI C'AATT_G Cuts at: 3300 3300 Size: 5701 MluI A'CGCG_T Cuts at: 2718 5577 2718 Size: 2859 2842 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 4815 4999 1305 Size: 3510 184 2007 Fragments arranged by size: 3510 2007 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 2405 10 Size: 54 1425 343 573 3306 Fragments arranged by size: 3306 1425 573 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 2651 3659 4018 4177 518 Size: 648 1485 1008 359 159 2042 Fragments arranged by size: 2042 1485 1008 648 359 159 MspA1I CmG'CkG Cuts at: 1606 3117 3794 4942 5187 1606 Size: 1511 677 1148 245 2120 Fragments arranged by size: 2120 1511 1148 677 245 NaeI GCC'GGC Cuts at: 2098 2098 Size: 5701 NarI GG'CG_CC Cuts at: 2045 2045 Size: 5701 NcoI C'CATG_G Cuts at: 513 1065 513 Size: 552 5149 NdeI CA'TA_TG Cuts at: 387 387 Size: 5701 NgoMIV G'CCGG_C Cuts at: 2096 2096 Size: 5701 NheI G'CTAG_C Cuts at: 1052 1052 Size: 5701 NotI GC'GGCC_GC Cuts at: 3148 3148 Size: 5701 NsiI A_TGCA'T Cuts at: 2532 2532 Size: 5701 NspI r_CATG'y Cuts at: 1132 1229 2426 2534 3059 4604 1132 Size: 97 1197 108 525 1545 2229 Fragments arranged by size: 2229 1545 1197 525 108 97 PasI CC'CwG_GG Cuts at: 1348 1348 Size: 5701 PciI A'CATG_T Cuts at: 4600 4600 Size: 5701 PflMI CCAn_nnn'nTGG Cuts at: 1108 1108 Size: 5701 PmeI GTTT'AAAC Cuts at: 3005 3005 Size: 5701 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 3770 3802 5283 5315 3770 Size: 32 1481 32 4156 Fragments arranged by size: 4156 1481 32 32 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 5701 PsiI TTA'TAA Cuts at: 3271 3271 Size: 5701 PstI C_TGCA'G Cuts at: 830 2190 3053 830 Size: 1360 863 3478 Fragments arranged by size: 3478 1360 863 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 5701 SalI G'TCGA_C Cuts at: 1936 3043 1936 Size: 1107 4594 SbfI CC_TGCA'GG Cuts at: 3053 3053 Size: 5701 ScaI AGT'ACT Cuts at: 3949 5661 3949 Size: 1712 3989 SexAI A'CCwGG_T Cuts at: 2577 2577 Size: 5701 SfcI C'TryA_G Cuts at: 826 1045 2186 3049 4184 4865 5056 826 Size: 219 1141 863 1135 681 191 1471 Fragments arranged by size: 1471 1141 1135 863 681 219 191 SfoI GGC'GCC Cuts at: 2046 2046 Size: 5701 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 5701 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 5701 SmaI CCC'GGG Cuts at: 1872 3028 1872 Size: 1156 4545 SmlI C'TyrA_G Cuts at: 820 1017 1943 3805 4706 4968 820 Size: 197 926 1862 901 262 1553 Fragments arranged by size: 1862 1553 926 901 262 197 SnaBI TAC'GTA Cuts at: 493 3492 493 Size: 2999 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 5701 SphI G_CATG'C Cuts at: 1132 2534 3059 1132 Size: 1402 525 3774 Fragments arranged by size: 3774 1402 525 SspI AAT'ATT Cuts at: 5 52 2359 3625 5 Size: 47 2307 1266 2081 Fragments arranged by size: 2307 2081 1266 47 StuI AGG'CCT Cuts at: 1517 2538 1517 Size: 1021 4680 StyI C'CwwG_G Cuts at: 513 1065 3140 513 Size: 552 2075 3074 Fragments arranged by size: 3074 2075 552 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 3728 3745 3898 4083 4528 325 Size: 1255 2148 17 153 185 445 1498 Fragments arranged by size: 2148 1498 1255 445 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 2442 3947 5659 Size: 274 80 33 51 1443 465 1505 1712 Cuts at: 5659 96 Size: 138 Fragments arranged by size: 1712 1505 1443 465 274 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 5661 5693 5661 Size: 32 5669 Tth111I GACn'n_nGTC Cuts at: 1316 2387 1316 Size: 1071 4630 XbaI T'CTAG_A Cuts at: 3037 3037 Size: 5701 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 5701 XmaI C'CCGG_G Cuts at: 1870 3026 1870 Size: 1156 4545 XmnI GAAnn'nnTTC Cuts at: 3830 3830 Size: 5701 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 3509 276 Size: 53 83 186 936 1975 2468 Fragments arranged by size: 2468 1975 936 186 83 53 Enzymes that do cut: AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbeI BbsI BbvCI BcgI BciVI BclI BfrBI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI BsmBI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI EarI EciI Eco57MI EcoNI EcoRI FspI HaeII Hin4I HincII HindIII HpaI KasI KpnI MfeI MluI MmeI MscI MslI MspA1I NaeI NarI NcoI NdeI NgoMIV NheI NotI NsiI NspI PasI PciI PflMI PmeI PpiI PshAI PsiI PstI PvuI SalI SbfI ScaI SexAI SfcI SfoI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XhoI XmaI XmnI ZraI Enzymes that do not cut: AarI AfeI AloI ApaI AscI AvrII BaeI BplI BsiWI BstZ17I Bsu36I DraIII EcoICRI EcoO109I EcoRV FalI FseI FspAI NruI PacI PfoI PmlI PpuMI PspOMI PspXI PsrI PvuII RsrII SacI SacII SanDI SapI SfiI SrfI SwaI XcmI