Restriction map for
pFN21AE1352
(Linear) (Six-base) MAPSORT of: KIEE1352.seq Check: 5419 from: 1 to: 996
KIEE1352.orf
With 182 enzymes: SgfI *
July 15, 2010 18:36 ..
AflIII A'CryG_T
Cuts at: 0 713 996
Size: 713 283
AleI CACnn'nnGTG
Cuts at: 0 646 996
Size: 646 350
ApoI r'AATT_y
Cuts at: 0 324 996
Size: 324 672
AvaI C'yCGr_G
Cuts at: 0 860 996
Size: 860 136
BanI G'GyrC_C
Cuts at: 0 39 313 996
Size: 39 274 683
Fragments arranged by size:
683 274 39
BanII G_rGCy'C
Cuts at: 0 68 996
Size: 68 928
BbeI G_GCGC'C
Cuts at: 0 43 996
Size: 43 953
BbsI GAAGACnn'nnnn_
Cuts at: 0 692 887 996
Size: 692 195 109
Fragments arranged by size:
692 195 109
BbvCI CC'TCA_GC
Cuts at: 0 902 996
Size: 902 94
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 309 324 343 358 996
Size: 309 15 19 15 638
Fragments arranged by size:
638 309 19 15 15
BciVI GTATCCnnnnn_n'
Cuts at: 0 850 996
Size: 850 146
BfrBI ATG'CAT
Cuts at: 0 525 996
Size: 525 471
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 647 684 996
Size: 647 37 312
Fragments arranged by size:
647 312 37
Bpu10I CC'TnA_GC
Cuts at: 0 166 902 996
Size: 166 736 94
Fragments arranged by size:
736 166 94
BsaI GGTCTCn'nnnn_
Cuts at: 0 591 996
Size: 591 405
BsaHI Gr'CG_yC
Cuts at: 0 40 95 996
Size: 40 55 901
Fragments arranged by size:
901 55 40
BsaWI w'CCGG_w
Cuts at: 0 776 996
Size: 776 220
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 681 919 943 996
Size: 681 238 24 53
Fragments arranged by size:
681 238 53 24
BseYI C'CCAG_C
Cuts at: 0 6 21 996
Size: 6 15 975
Fragments arranged by size:
975 15 6
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 73 283 996
Size: 73 210 713
Fragments arranged by size:
713 210 73
BsmBI CGTCTCn'nnnn_
Cuts at: 0 127 996
Size: 127 869
Bsp1286I G_dGCh'C
Cuts at: 0 68 996
Size: 68 928
BsrDI GCAATG_nn'
Cuts at: 0 403 627 996
Size: 403 224 369
Fragments arranged by size:
403 369 224
BsrFI r'CCGG_y
Cuts at: 0 91 103 316 996
Size: 91 12 213 680
Fragments arranged by size:
680 213 91 12
BsrGI T'GTAC_A
Cuts at: 0 437 996
Size: 437 559
BstXI CCAn_nnnn'nTGG
Cuts at: 0 75 996
Size: 75 921
BstYI r'GATC_y
Cuts at: 0 189 996
Size: 189 807
BtgI C'CryG_G
Cuts at: 0 47 996
Size: 47 949
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 0 327 996
Size: 327 669
BtsI GCAGTG_nn'
Cuts at: 0 178 996
Size: 178 818
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 0 729 764 996
Size: 729 35 232
Fragments arranged by size:
729 232 35
EaeI y'GGCC_r
Cuts at: 0 89 398 996
Size: 89 309 598
Fragments arranged by size:
598 309 89
EarI CTCTTCn'nnn_
Cuts at: 0 139 996
Size: 139 857
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 647 684 996
Size: 647 37 312
Fragments arranged by size:
647 312 37
EcoNI CCTnn'n_nnAGG
Cuts at: 0 486 996
Size: 486 510
HaeII r_GCGC'y
Cuts at: 0 43 996
Size: 43 953
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 571 603 996
Size: 571 32 393
Fragments arranged by size:
571 393 32
KasI G'GCGC_C
Cuts at: 0 39 996
Size: 39 957
MluI A'CGCG_T
Cuts at: 0 713 996
Size: 713 283
MscI TGG'CCA
Cuts at: 0 400 996
Size: 400 596
MslI CAynn'nnrTG
Cuts at: 0 646 996
Size: 646 350
NaeI GCC'GGC
Cuts at: 0 93 996
Size: 93 903
NarI GG'CG_CC
Cuts at: 0 40 996
Size: 40 956
NgoMIV G'CCGG_C
Cuts at: 0 91 996
Size: 91 905
NsiI A_TGCA'T
Cuts at: 0 527 996
Size: 527 469
NspI r_CATG'y
Cuts at: 0 421 529 996
Size: 421 108 467
Fragments arranged by size:
467 421 108
PstI C_TGCA'G
Cuts at: 0 185 996
Size: 185 811
SexAI A'CCwGG_T
Cuts at: 0 572 996
Size: 572 424
SfcI C'TryA_G
Cuts at: 0 181 996
Size: 181 815
SfoI GGC'GCC
Cuts at: 0 41 996
Size: 41 955
SphI G_CATG'C
Cuts at: 0 529 996
Size: 529 467
SspI AAT'ATT
Cuts at: 0 354 996
Size: 354 642
StuI AGG'CCT
Cuts at: 0 533 996
Size: 533 463
TatI w'GTAC_w
Cuts at: 0 437 996
Size: 437 559
Tth111I GACn'n_nGTC
Cuts at: 0 382 996
Size: 382 614
Enzymes that do cut:
AflIII AleI ApoI AvaI BanI BanII BbeI BbsI
BbvCI BcgI BciVI BfrBI BpmI Bpu10I BsaI BsaHI
BsaWI BseRI BseYI BsgI BsmBI Bsp1286I BsrDI BsrFI
BsrGI BstXI BstYI BtgI BtgZI BtsI CspCI EaeI
EarI Eco57MI EcoNI HaeII Hin4I KasI MluI MscI
MslI NaeI NarI NgoMIV NsiI NspI PstI SexAI
SfcI SfoI SphI SspI StuI TatI Tth111I
Enzymes that do not cut:
AarI AatII AccI Acc65I AclI AcuI AfeI AflII
AgeI AhdI AlfI AloI AlwNI ApaI ApaLI AscI
AseI AsiSI AvrII BaeI BamHI BclI BglI BglII
BlpI Bme1580I BmgBI BmrI BmtI BplI BpuEI BsaAI
BsaBI BsaXI BsiEI BsiHKAI BsiWI BsmI BspEI BspHI
BspMI BsrBI BssHII BssSI BstAPI BstBI BstEII BstZ17I
Bsu36I ClaI DraI DraIII DrdI EagI EciI EcoICRI
EcoO109I EcoRI EcoRV FalI FseI FspI FspAI HincII
HindIII HpaI KpnI MfeI MmeI MspA1I NcoI NdeI
NheI NotI NruI PacI PasI PciI PflMI PfoI
PmeI PmlI PpiI PpuMI PshAI PsiI PspOMI PspXI
PsrI PvuI PvuII RsrII SacI SacII SalI SanDI
SapI SbfI ScaI SfiI SgfI SgrAI SmaI SmlI
SnaBI SpeI SrfI StyI SwaI TaqII TstI XbaI
XcmI XhoI XmaI XmnI ZraI