################################## # NO CUT ENZYMES ( 119 enzymes) # ################################## AatII Acc65I AccI AccII AccIII AcyI AflII AflIII AgeI AhdI Alw44I AocI ApaI ApaLI AscI Asp718I AspEI AspHI AspI AviII AvrII BbeI BbrPI BbvCI BciVI BclI BfrI BglI BlnI BlpI Bpu1102I BsaHI BsaWI BseAI BseRI BsgI BsiEI BsiHKAI BsmBI BsmI BspEI BspHI BspLU11I BspMI BsrFI BssHII BssSI Bst1107I BstUI BstZ17I Bsu36I CelII Cfr10I CpoI EagI Eam1105I EclXI Eco52I Eco81I EcoNI EcoT22I FauI FbaI FseI FspI HgaI Hin1I KasI KpnI KspI MfeI MluI MroI MslI MspA1I MunI MvnI NaeI NarI NdeI NgoMIV NheI NotI NruI NsiI PacI PflFI PflMI PinAI PmaCI PmeI PmlI PshAI PvuI PvuII RcaI RsrII SacI SacII SalI SapI SbfI ScaI SexAI SfiI SfoI SgrAI SmaI SnaBI SpeI Sse8387I StuI SwaI Tth111I Van91I XbaI XcmI XmaCI XmaI ################################### # ONE CUT ENZYMES ( 65 enzymes) # ################################### AclI 61 AACGTT AA^CGTT Aor51HI 1166 AGCGCT AGC^GCT AseI 445 ATTAAT AT^TAAT AsnI 445 ATTAAT AT^TAAT Asp700I 266 GAACTACTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC BalI 992 TGGCCA TGG^CCA BamHI 661 GGATCC G^GATCC BanI 844 GGTGCC G^G[CT][GA]CC BanII 1655 GAGCCC G[GA]GC[CT]^C BbsI 468 GTCTTC GAAGAC(2/6) BcgI 364 CGACTTGCTTGC (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10) BcnI 1228 CCCGG CC^[GC]GG BfaI 1354 CTAG C^TAG BglII 1383 AGATCT A^GATCT BmyI 1655 GAGCCC G[AGT]GC[ACT]^C BpuAI 468 GTCTTC GAAGAC(2/6) BsaAI 1130 TACGTG [CT]AC^GT[GA] BsaBI 1083 GATCTTAATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC BsaI 1292 GGTCTC GGTCTC(1/5) BsiWI 1108 CGTACG C^GTACG Bsp1286I 1655 GAGCCC G[AGT]GC[ACT]^C Bsp1407I 747 TGTACA T^GTACA BspDI 1119 ATCGAT AT^CGAT BsrBI 51 CCGCTC CCGCTC(-3/-3) BsrGI 747 TGTACA T^GTACA BstBI 1574 TTCGAA TT^CGAA BstEII 25 GGTGACC G^GT[ACGT]ACC BstPI 25 GGTGACC G^GT[ACGT]ACC BstXI 1379 CCACAGATCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG CfoI 1167 GCGC GCG^C ClaI 1119 ATCGAT AT^CGAT DraI 933 TTTAAA TTT^AAA DraIII 1184 CACTTTGTG CAC[ACGT][ACGT][ACGT]^GTG DrdI 459 GACATCCATGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC DsaI 600 CCATGG C^C[GA][CT]GG EaeI 992 TGGCCA [CT]^GGCC[GA] Eco47III 1166 AGCGCT AGC^GCT EcoO65I 25 GGTGACC G^GT[ACGT]ACC EcoRI 821 GAATTC G^AATTC EcoRV 805 GATATC GAT^ATC HaeII 1166 AGCGCT [GA]GCGC^[CT] HapII 1229 CCGG C^CGG HhaI 1167 GCGC GCG^C HinP1I 1167 GCGC G^CGC HpaII 1229 CCGG C^CGG MaeI 1354 CTAG C^TAG MamI 1083 GATCTTAATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC MluNI 992 TGGCCA TGG^CCA MscI 992 TGGCCA TGG^CCA MspI 1229 CCGG C^CGG NciI 1228 CCCGG CC^[GC]GG NcoI 600 CCATGG C^CATGG NspI 671 GCATGC [GA]CATG^[CT] NspV 1574 TTCGAA TT^CGAA PaeR7I 1484 CTCGAG C^TCGAG PpuMI 941 GGGACCT [GA]G^G[AT]CC[CT] PshBI 445 ATTAAT AT^TAAT Psp1406I 61 AACGTT AA^CGTT SfuI 1574 TTCGAA TT^CGAA SmlI 1484 CTCGAG C^T[CT][GA]AG SphI 671 GCATGC GCATG^C SspBI 747 TGTACA T^GTACA Tsp45I 26 GTGAC ^GT[GC]AC XhoI 1484 CTCGAG C^TCGAG XmnI 266 GAACTACTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC ################################### # TWO CUT ENZYMES ( 19 enzymes) # ################################### AciI 30 CCGC CCGC(-3/-1) 51 CCGC AlwNI 764 CAGACCCTG CAG[ACGT][ACGT][ACGT]^CTG 1553 CAGAAGCTG AvaI 1295 CTCGGG C^[CT]CG[GA]G 1484 CTCGAG BsiYI 1228 CCCGGTGGGGG CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG 1283 CCAGCATGGGG BslI 1228 CCCGGTGGGGG CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG 1283 CCAGCATGGGG BsmAI 1487 GAGAC GTCTC(1/5) 1293 GTCTC BsmFI 941 GGGAC GGGAC(10/14) 1035 GGGAC BsoBI 1295 CTCGGG C^[CT]CG[GA]G 1484 CTCGAG DdeI 455 CTAAG C^T[ACGT]AG 1100 CTAAG DraII 6 GGGGCCT [GA]G^G[ACGT]CC[CT] 941 GGGACCT EcoO109I 6 GGGGCCT [GA]G^G[ACGT]CC[CT] 941 GGGACCT EcoT14I 600 CCATGG C^C[AT][AT]GG 812 CCAAGG HindIII 739 AAGCTT A^AGCTT 962 AAGCTT HpaI 412 GTTAAC GTT^AAC 1426 GTTAAC PleI 1375 GACTC GAGTC(4/5) 1210 GAGTC PstI 71 CTGCAG CTGCA^G 209 CTGCAG SspI 88 AATATT AAT^ATT 586 AATATT StyI 600 CCATGG C^C[AT][AT]GG 812 CCAAGG TfiI 683 GAATC G^A[AT]TC 799 GAATC ######################################### # 3 OR MORE CUT ENZYMES ( 55 enzymes) # ######################################### AcsI AfaI AluI AlwI ApoI AvaII BbvI BpmI BsaJI BsrDI BsrI BssKI BstAPI BstF5I BstNI BstYI Cac8I Cfr13I DpnII EarI EcoRII Fnu4HI FokI HaeIII HincII HindII HinfI HphI ItaI Ksp632I MaeII MaeIII MboI MboII MflI MnlI MseI MvaI MwoI NdeII NlaIII NlaIV RsaI Sau3AI Sau96I ScrFI SfaNI SfcI TaqI Tru9I TseI Tsp509I TspRI TthHB8I XhoII