(Circular) (Six-base) MAPSORT of: pFN21AE1424.seq Check: 966 from: 1 to: 6373 pFN21AE1424 6373 bp With 182 enzymes: SgfI * July 6, 2012 17:35 .. AatII G_ACGT'C Cuts at: 278 331 414 600 1536 4183 278 Size: 53 83 186 936 2647 2468 Fragments arranged by size: 2647 2468 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 3716 1937 Size: 1779 4594 Acc65I G'GTAC_C Cuts at: 3694 3694 Size: 6373 AclI AA'CG_TT Cuts at: 1563 2067 4500 4873 1563 Size: 504 2433 373 3063 Fragments arranged by size: 3063 2433 504 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 2210 2262 2704 2915 4436 5820 6088 1632 Size: 578 52 442 211 1521 1384 268 1917 Fragments arranged by size: 1917 1521 1384 578 442 268 211 52 AfeI AGC'GCT Cuts at: 3173 3173 Size: 6373 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 6176 AflIII A'CryG_T Cuts at: 1203 5272 6249 1203 Size: 4069 977 1327 Fragments arranged by size: 4069 1327 977 AgeI A'CCGG_T Cuts at: 5204 5204 Size: 6373 AhdI GACnn_n'nnGTC Cuts at: 5102 5102 Size: 6373 AleI CACnn'nnGTG Cuts at: 1166 1166 Size: 6373 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 1184 Size: 6373 AlwNI CAG_nnn'CTG Cuts at: 2774 3563 4116 5688 2774 Size: 789 553 1572 3459 Fragments arranged by size: 3459 1572 789 553 ApaLI G'TGCA_C Cuts at: 4430 5586 4430 Size: 1156 5217 ApoI r'AATT_y Cuts at: 1493 2337 2826 3069 3548 3682 3908 4060 6169 Size: 844 489 243 479 134 226 152 2109 Cuts at: 6169 1493 Size: 1697 Fragments arranged by size: 2109 1697 844 489 479 243 226 152 134 AseI AT'TA_AT Cuts at: 160 1032 2451 4927 160 Size: 872 1419 2476 1606 Fragments arranged by size: 2476 1606 1419 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 6373 AvaI C'yCGr_G Cuts at: 1870 1943 3300 3489 3698 1870 Size: 73 1357 189 209 4545 Fragments arranged by size: 4545 1357 209 189 73 BamHI G'GATC_C Cuts at: 2666 3703 4070 2666 Size: 1037 367 4969 Fragments arranged by size: 4969 1037 367 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2849 3694 5149 618 Size: 325 217 627 1062 845 1455 1842 Fragments arranged by size: 1842 1455 1062 845 627 325 217 BanII G_rGCy'C Cuts at: 1391 3664 3692 1391 Size: 2273 28 4072 Fragments arranged by size: 4072 2273 28 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 2466 928 Size: 971 567 4835 Fragments arranged by size: 4835 971 567 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 2358 2392 4564 4598 1903 Size: 34 421 34 2172 34 3678 Fragments arranged by size: 3678 2172 421 34 34 34 BciVI GTATCCnnnnn_n' Cuts at: 4265 5475 4265 Size: 1210 5163 BclI T'GATC_A Cuts at: 1548 1548 Size: 6373 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 4984 136 Size: 107 122 71 631 605 3312 1525 Fragments arranged by size: 3312 1525 631 605 122 107 71 BglII A'GATC_T Cuts at: 3388 6368 3388 Size: 2980 3393 BlpI GC'TnA_GC Cuts at: 3790 3790 Size: 6373 Bme1580I G_kGCm'C Cuts at: 1790 4434 5590 1790 Size: 2644 1156 2573 Fragments arranged by size: 2644 2573 1156 BmgBI CAC'GTC Cuts at: 1318 1318 Size: 6373 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 2052 2441 4104 Size: 416 53 83 399 24 635 389 1663 Cuts at: 4104 5062 442 Size: 958 1753 Fragments arranged by size: 1753 1663 958 635 416 399 389 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 6373 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2219 2994 3678 5033 1239 Size: 980 775 684 1355 2579 Fragments arranged by size: 2579 1355 980 775 684 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 4498 5363 5661 4498 Size: 865 298 5210 Fragments arranged by size: 5210 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 3303 5036 882 Size: 617 1804 1733 2219 Fragments arranged by size: 2219 1804 1733 617 BsaAI yAC'GTr Cuts at: 493 1204 3137 4164 493 Size: 711 1933 1027 2702 Fragments arranged by size: 2702 1933 1027 711 BsaBI GATnn'nnATC Cuts at: 3092 6367 3092 Size: 3275 3098 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 4180 4562 5197 275 Size: 53 83 186 936 2647 382 635 1451 Fragments arranged by size: 2647 1451 936 635 382 186 83 53 BsaWI w'CCGG_w Cuts at: 4806 5204 5478 5625 6036 4806 Size: 398 274 147 411 5143 Fragments arranged by size: 5143 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 1178 Size: 30 165 30 6148 Fragments arranged by size: 6148 165 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 1186 Size: 6373 BseYI C'CCAG_C Cuts at: 5576 6072 5576 Size: 496 5877 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 1163 Size: 573 5800 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 3823 4584 5612 1540 Size: 275 186 1822 761 1028 2301 Fragments arranged by size: 2301 1822 1028 761 275 186 BsiHKAI G_wGCw'C Cuts at: 4434 4519 5590 4434 Size: 85 1071 5217 Fragments arranged by size: 5217 1071 85 BsiWI C'GTAC_G Cuts at: 3113 3113 Size: 6373 BsmI GAATG_Cn' Cuts at: 3884 3977 3884 Size: 93 6280 Bsp1286I G_dGCh'C Cuts at: 1391 1790 3664 3692 4434 4519 5590 1391 Size: 399 1874 28 742 85 1071 2174 Fragments arranged by size: 2174 1874 1071 742 399 85 28 BspEI T'CCGG_A Cuts at: 6036 6036 Size: 6373 BspHI T'CATG_A Cuts at: 4262 5992 4262 Size: 1730 4643 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 3728 844 Size: 2884 3489 BsrBI CCG'CTC Cuts at: 1394 1823 2058 4260 1394 Size: 429 235 2202 3507 Fragments arranged by size: 3507 2202 429 235 BsrDI GCAATG_nn' Cuts at: 65 1248 2958 3039 3484 4868 5042 65 Size: 1183 1710 81 445 1384 174 1396 Fragments arranged by size: 1710 1396 1384 1183 445 174 81 BsrFI r'CCGG_y Cuts at: 1700 1810 5017 5204 1700 Size: 110 3207 187 2869 Fragments arranged by size: 3207 2869 187 110 BsrGI T'GTAC_A Cuts at: 96 2752 96 Size: 2656 3717 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 6373 BssSI C'ACGA_G Cuts at: 4433 5445 4433 Size: 1012 5361 BstAPI GCAn_nnn'nTGC Cuts at: 794 2705 2849 3326 3368 794 Size: 1911 144 477 42 3799 Fragments arranged by size: 3799 1911 477 144 42 BstBI TT'CG_AA Cuts at: 3580 5177 3580 Size: 1597 4776 BstEII G'GTnAC_C Cuts at: 1184 2030 1184 Size: 846 5527 BstXI CCAn_nnnn'nTGG Cuts at: 3391 3391 Size: 6373 BstYI r'GATC_y Cuts at: 1972 2666 2835 3388 3703 4070 4455 4472 5924 Size: 694 169 553 315 367 385 17 1452 Cuts at: 5924 6010 6022 6368 1972 Size: 86 12 346 1977 Fragments arranged by size: 1977 1452 694 553 385 367 346 315 169 86 17 12 BtgI C'CryG_G Cuts at: 513 1065 2004 2605 513 Size: 552 939 601 4281 Fragments arranged by size: 4281 939 601 552 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 487 Size: 683 56 5634 Fragments arranged by size: 5634 683 56 BtsI GCAGTG_nn' Cuts at: 2394 3889 4683 4703 2394 Size: 1495 794 20 4064 Fragments arranged by size: 4064 1495 794 20 ClaI AT'CG_AT Cuts at: 1554 3125 1554 Size: 1571 4802 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 3207 3242 529 Size: 35 2643 35 3660 Fragments arranged by size: 3660 2643 35 35 DraI TTT'AAA Cuts at: 2940 3677 2940 Size: 737 5636 DraIII CAC_nnn'GTG Cuts at: 3194 3194 Size: 6373 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 2470 5380 809 Size: 507 1154 2910 1802 Fragments arranged by size: 2910 1802 1154 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2997 3820 4709 Size: 54 1425 50 275 18 1167 823 889 Cuts at: 4709 6338 8 Size: 1629 43 Fragments arranged by size: 1629 1425 1167 889 823 275 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 3820 1537 Size: 275 2008 4090 Fragments arranged by size: 4090 2008 275 EarI CTCTTCn'nnn_ Cuts at: 1353 2431 2926 3197 4303 1353 Size: 1078 495 271 1106 3423 Fragments arranged by size: 3423 1106 1078 495 271 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 4960 5334 5480 387 Size: 1064 138 3371 374 146 1280 Fragments arranged by size: 3371 1280 1064 374 146 138 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2210 2219 2262 2704 2915 2994 3678 Size: 393 578 9 43 442 211 79 684 Cuts at: 3678 4436 5033 5820 6088 1239 Size: 758 597 787 268 1524 Fragments arranged by size: 1524 787 758 684 597 578 442 393 268 211 79 43 9 EcoO109I rG'GnC_Cy Cuts at: 2012 2947 2012 Size: 935 5438 EcoRI G'AATT_C Cuts at: 2826 3682 4060 2826 Size: 856 378 5139 Fragments arranged by size: 5139 856 378 EcoRV GAT'ATC Cuts at: 2812 2812 Size: 6373 FalI AAGnnnnnCTTnnnnnnnn_nnnnn' Cuts at: 3275 3275 Size: 6373 FspI TGC'GCA Cuts at: 4879 4879 Size: 6373 HaeII r_GCGC'y Cuts at: 1678 3175 5520 1678 Size: 1497 2345 2531 Fragments arranged by size: 2531 2345 1497 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 3206 3238 5014 5046 5088 Size: 32 135 32 1634 32 1776 32 42 Cuts at: 5088 5120 1373 Size: 32 2626 Fragments arranged by size: 2626 1776 1634 135 42 32 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 2419 2581 2991 3433 3717 3963 Size: 988 281 481 162 410 442 284 246 Cuts at: 3963 669 Size: 3079 Fragments arranged by size: 3079 988 481 442 410 284 281 246 162 HindIII A'AGCT_T Cuts at: 748 2744 2967 748 Size: 1996 223 4154 Fragments arranged by size: 4154 1996 223 HpaI GTT'AAC Cuts at: 2419 3433 3963 2419 Size: 1014 530 4829 Fragments arranged by size: 4829 1014 530 KpnI G_GTAC'C Cuts at: 3698 3698 Size: 6373 MfeI C'AATT_G Cuts at: 3972 3972 Size: 6373 MluI A'CGCG_T Cuts at: 6249 6249 Size: 6373 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 2242 5487 5671 1305 Size: 937 3245 184 2007 Fragments arranged by size: 3245 2007 937 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 2999 10 Size: 54 1425 343 1167 3384 Fragments arranged by size: 3384 1425 1167 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 4331 4690 4849 518 Size: 648 3165 359 159 2042 Fragments arranged by size: 3165 2042 648 359 159 MspA1I CmG'CkG Cuts at: 1606 3789 4466 5614 5859 1606 Size: 2183 677 1148 245 2120 Fragments arranged by size: 2183 2120 1148 677 245 NcoI C'CATG_G Cuts at: 513 1065 2004 2605 513 Size: 552 939 601 4281 Fragments arranged by size: 4281 939 601 552 NdeI CA'TA_TG Cuts at: 387 387 Size: 6373 NheI G'CTAG_C Cuts at: 1052 1052 Size: 6373 NotI GC'GGCC_GC Cuts at: 3820 3820 Size: 6373 NspI r_CATG'y Cuts at: 1132 1229 2680 3731 5276 1132 Size: 97 1451 1051 1545 2229 Fragments arranged by size: 2229 1545 1451 1051 97 PasI CC'CwG_GG Cuts at: 1348 2671 1348 Size: 1323 5050 PciI A'CATG_T Cuts at: 5272 5272 Size: 6373 PflMI CCAn_nnn'nTGG Cuts at: 1108 1108 Size: 6373 PmeI GTTT'AAAC Cuts at: 3677 3677 Size: 6373 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 4442 4474 5955 5987 4442 Size: 32 1481 32 4828 Fragments arranged by size: 4828 1481 32 32 PpuMI rG'GwC_Cy Cuts at: 2947 2947 Size: 6373 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 6373 PsiI TTA'TAA Cuts at: 3943 3943 Size: 6373 PstI C_TGCA'G Cuts at: 830 2080 2218 3725 830 Size: 1250 138 1507 3478 Fragments arranged by size: 3478 1507 1250 138 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 6373 SalI G'TCGA_C Cuts at: 1936 3715 1936 Size: 1779 4594 SbfI CC_TGCA'GG Cuts at: 3725 3725 Size: 6373 ScaI AGT'ACT Cuts at: 4621 6333 4621 Size: 1712 4661 SfcI C'TryA_G Cuts at: 826 1045 2076 2214 3246 3721 4856 5537 5728 Size: 219 1031 138 1032 475 1135 681 191 Cuts at: 5728 826 Size: 1471 Fragments arranged by size: 1471 1135 1032 1031 681 475 219 191 138 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 6373 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 6373 SmaI CCC'GGG Cuts at: 1872 3700 1872 Size: 1828 4545 SmlI C'TyrA_G Cuts at: 820 1017 1943 3489 4477 5378 5640 820 Size: 197 926 1546 988 901 262 1553 Fragments arranged by size: 1553 1546 988 926 901 262 197 SnaBI TAC'GTA Cuts at: 493 4164 493 Size: 3671 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 6373 SphI G_CATG'C Cuts at: 1132 2680 3731 1132 Size: 1548 1051 3774 Fragments arranged by size: 3774 1548 1051 SspI AAT'ATT Cuts at: 5 52 2095 2593 4297 5 Size: 47 2043 498 1704 2081 Fragments arranged by size: 2081 2043 1704 498 47 StuI AGG'CCT Cuts at: 1517 1517 Size: 6373 StyI C'CwwG_G Cuts at: 513 1065 2004 2605 2817 3812 513 Size: 552 939 601 212 995 3074 Fragments arranged by size: 3074 995 939 601 552 212 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 4400 4417 4570 4755 5200 325 Size: 1255 2820 17 153 185 445 1498 Fragments arranged by size: 2820 1498 1255 445 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 2530 2752 4619 Size: 274 80 33 51 1443 553 222 1867 Cuts at: 4619 6331 96 Size: 1712 138 Fragments arranged by size: 1867 1712 1443 553 274 222 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 6333 6365 6333 Size: 32 6341 Tth111I GACn'n_nGTC Cuts at: 1316 1316 Size: 6373 XbaI T'CTAG_A Cuts at: 3709 3709 Size: 6373 XhoI C'TCGA_G Cuts at: 1943 3489 1943 Size: 1546 4827 XmaI C'CCGG_G Cuts at: 1870 3698 1870 Size: 1828 4545 XmnI GAAnn'nnTTC Cuts at: 2275 4502 2275 Size: 2227 4146 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 4181 276 Size: 53 83 186 936 2647 2468 Fragments arranged by size: 2647 2468 936 186 83 53 Enzymes that do cut: AatII AccI Acc65I AclI AcuI AfeI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbsI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BpmI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsiWI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI BtgI BtgZI BtsI ClaI CspCI DraI DraIII DrdI EaeI EagI EarI EciI Eco57MI EcoO109I EcoRI EcoRV FalI FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I NcoI NdeI NheI NotI NspI PasI PciI PflMI PmeI PpiI PpuMI PshAI PsiI PstI PvuI SalI SbfI ScaI SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XhoI XmaI XmnI ZraI Enzymes that do not cut: AarI AloI ApaI AscI AvrII BaeI BbeI BbvCI BfrBI BplI Bpu10I BsmBI BstZ17I Bsu36I EcoICRI EcoNI FseI FspAI KasI NaeI NarI NgoMIV NruI NsiI PacI PfoI PmlI PspOMI PspXI PsrI PvuII RsrII SacI SacII SanDI SapI SexAI SfiI SfoI SrfI SwaI XcmI