################################## # NO CUT ENZYMES ( 173 enzymes) # ################################## AatII Acc65I AccI AccII AccIII AclI AfaI AflII AflIII AgeI Alw44I AlwNI AocI Aor51HI ApaI ApaLI AscI AseI AsnI Asp700I Asp718I AspI AviII AvrII BalI BamHI BanI BanII BbeI BbrPI BbsI BcgI BciVI BclI BcnI BfaI BfrI BglI BglII BlnI BlpI BpmI Bpu1102I BpuAI BsaAI BsaI BsaWI BseAI BseRI BsgI BsiEI BsiWI BsmAI BsmBI BsmI Bsp1407I BspDI BspEI BspHI BspLU11I BsrBI BsrDI BsrFI BsrGI BssHII BssSI Bst1107I BstBI BstEII BstF5I BstPI BstUI BstXI BstZ17I Bsu36I CelII CfoI Cfr10I ClaI CpoI DraI DrdI DsaI EaeI EagI EclXI Eco47III Eco52I Eco81I EcoO65I EcoRI EcoRV EcoT22I FbaI FokI FseI FspI HaeII HapII HhaI HinP1I HpaI HpaII KasI KpnI KspI MaeI MaeIII MfeI MluI MluNI MroI MscI MspA1I MspI MunI MvnI NaeI NarI NciI NcoI NdeI NgoMIV NheI NotI NruI NsiI NspI NspV PacI PflFI PflMI PinAI PleI PmaCI PmeI PmlI PshAI PshBI Psp1406I PstI PvuI PvuII RcaI RsaI RsrII SacI SacII SalI SbfI ScaI SfaNI SfiI SfoI SfuI SgrAI SmaI SnaBI SpeI SphI Sse8387I SspBI SspI StuI SwaI Tsp45I Tth111I Van91I XbaI XcmI XmaCI XmaI XmnI ################################### # ONE CUT ENZYMES ( 62 enzymes) # ################################### AciI 9 CCGC CCGC(-3/-1) AcyI 19 GGCGTC G[GA]^CG[CT]C AhdI 135 GACTGAACGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC AlwI 272 GATCC GGATC(4/5) AspEI 135 GACTGAACGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC AspHI 199 GTGCTC G[AT]GC[AT]^C AvaI 149 CTCGAG C^[CT]CG[GA]G AvaII 500 GGACC G^G[AT]CC BbvCI 85 GCTGAGG CCTCAGC(-5/-2) BbvI 11 GCAGC GCAGC(8/12) BmyI 199 GTGCTC G[AGT]GC[ACT]^C BsaBI 601 GATTTGAATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC BsaHI 19 GGCGTC G[GA]^CG[CT]C BsiHKAI 199 GTGCTC G[AT]GC[AT]^C BsiYI 443 CCTTGGTCAGG CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG BslI 443 CCTTGGTCAGG CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG BsmFI 335 GGGAC GGGAC(10/14) BsoBI 149 CTCGAG C^[CT]CG[GA]G Bsp1286I 199 GTGCTC G[AGT]GC[ACT]^C BspMI 230 ACCTGC ACCTGC(4/8) BstAPI 429 GCAGACTTTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC BstYI 271 AGATCC [GA]^GATC[CT] Cfr13I 500 GGACC G^G[ACGT]CC DdeI 86 CTGAG C^T[ACGT]AG DraII 499 AGGACCC [GA]G^G[ACGT]CC[CT] DraIII 506 CACGAAGTG CAC[ACGT][ACGT][ACGT]^GTG Eam1105I 135 GACTGAACGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC EarI 306 GAAGAG CTCTTC(1/4) EcoNI 443 CCTTGGTCAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG EcoO109I 499 AGGACCC [GA]G^G[ACGT]CC[CT] EcoT14I 443 CCTTGG C^C[AT][AT]GG FauI 8 CCCGC CCCGC(4/6) Fnu4HI 11 GCAGC GC^[ACGT]GC HaeIII 175 GGCC GG^CC HgaI 20 GCGTC GACGC(5/10) Hin1I 19 GGCGTC G[GA]^CG[CT]C HincII 327 GTCAAC GT[CT]^[GA]AC HindII 327 GTCAAC GT[CT]^[GA]AC HindIII 483 AAGCTT A^AGCTT HinfI 606 GAATC G^A[ACGT]TC HphI 492 GGTGA GGTGA(8/7) ItaI 11 GCAGC GC^[ACGT]GC Ksp632I 306 GAAGAG CTCTTC(1/4) MaeII 141 ACGT A^CGT MamI 601 GATTTGAATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC MflI 271 AGATCC [GA]^GATC[CT] MseI 373 TTAA T^TAA NlaIV 500 GGACCC GG[ACGT]^[ACGT]CC PaeR7I 149 CTCGAG C^TCGAG PpuMI 499 AGGACCC [GA]G^G[AT]CC[CT] SapI 306 GAAGAGC GCTCTTC(1/4) Sau96I 500 GGACC G^G[ACGT]CC SexAI 42 ACCAGGT A^CC[AT]GGT SfcI 287 CTGTAG C^T[GA][CT]AG StyI 443 CCTTGG C^C[AT][AT]GG TaqI 150 TCGA T^CGA TfiI 606 GAATC G^A[AT]TC Tru9I 373 TTAA T^TAA TseI 11 GCAGC G^C[AT]GC TthHB8I 150 TCGA T^CGA XhoI 149 CTCGAG C^TCGAG XhoII 271 AGATCC [GA]^GATC[CT] ################################### # TWO CUT ENZYMES ( 20 enzymes) # ################################### AcsI 217 AAATTC [GA]^AATT[CT] 545 AAATTC AluI 310 AGCT AG^CT 484 AGCT ApoI 217 AAATTC [GA]^AATT[CT] 545 AAATTC BsaJI 15 CCCTGG C^C[ACGT][ACGT]GG 443 CCTTGG BssKI 16 CCTGG ^CC[ACGT]GG 43 CCAGG BstNI 16 CCTGG CC^[AT]GG 43 CCAGG Cac8I 481 GCAAGC GC[ACGT]^[ACGT]GC 485 GCTTGC DpnII 272 GATC ^GATC 361 GATC EcoRII 16 CCTGG ^CC[AT]GG 43 CCAGG MboI 272 GATC ^GATC 361 GATC MboII 306 GAAGA GAAGA(8/7) 400 GAAGA MslI 276 CATTCCTATG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG 610 CATGTTTGTG MvaI 16 CCTGG CC^[AT]GG 43 CCAGG MwoI 11 GCAGCCCTGGC GC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GC 429 GCAGACTTTGC NdeII 272 GATC ^GATC 361 GATC NlaIII 115 CATG CATG^ 610 CATG Sau3AI 272 GATC ^GATC 361 GATC ScrFI 16 CCTGG CC^[ACGT]GG 43 CCAGG SmlI 149 CTCGAG C^T[CT][GA]AG 202 CTCAAG TspRI 251 CACTG CA[GC]TG[ACGT][ACGT]^ 414 CACTG ######################################### # 3 OR MORE CUT ENZYMES ( 3 enzymes) # ######################################### BsrI MnlI Tsp509I