Restriction map for
pFN21AE2789
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# NO CUT ENZYMES ( 161 enzymes) #
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AatII Acc65I AccI AccIII AclI
AcsI AfaI AflII AflIII AgeI
AhdI Alw44I AlwI AocI Aor51HI
ApaLI ApoI AscI AseI AsnI
Asp700I Asp718I AspEI AspHI AspI
AviII AvrII BalI BamHI BbrPI
BcgI BciVI BclI BfrI BglII
BlnI BpmI BsaAI BsaBI BsaWI
BseAI BsgI BsiHKAI BsiWI BsmBI
BsmI Bsp1407I BspDI BspEI BspHI
BspLU11I BspMI BsrBI BsrDI BsrGI
BssHII BssSI Bst1107I BstBI BstEII
BstF5I BstPI BstYI BstZ17I Bsu36I
ClaI CpoI DpnII DraI DraIII
DrdI Eam1105I Eco47III Eco81I EcoNI
EcoO65I EcoRI EcoRV EcoT22I FbaI
FokI FseI FspI HincII HindII
HindIII HpaI HphI KpnI MaeII
MamI MboI MfeI MflI MluI
MluNI MroI MscI MseI MslI
MunI NaeI NcoI NdeI NdeII
NgoMIV NheI NlaIII NruI NsiI
NspI NspV PacI PaeR7I PflFI
PflMI PinAI PmaCI PmeI PmlI
PshAI PshBI Psp1406I PvuI PvuII
RcaI RsaI RsrII SacI SalI
Sau3AI SbfI ScaI SexAI SfaNI
SfuI SgrAI SmaI SmlI SnaBI
SpeI SphI Sse8387I SspBI SspI
StuI SwaI TaqI TfiI Tru9I
TspRI Tth111I TthHB8I Van91I XbaI
XcmI XhoI XhoII XmaCI XmaI
XmnI
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# ONE CUT ENZYMES ( 23 enzymes) #
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AvaII 104 GGACC G^G[AT]CC
BbsI 264 GAAGAC GAAGAC(2/6)
BbvCI 367 GCTGAGG CCTCAGC(-5/-2)
BfaI 578 CTAG C^TAG
BpuAI 264 GAAGAC GAAGAC(2/6)
BsmFI 103 GGGAC GGGAC(10/14)
BsrFI 630 GCCGGT [GA]^CCGG[CT]
BsrI 451 ACTGG ACTGG(1/-1)
BstXI 626 CCAAGCCGGTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
Cfr10I 630 GCCGGT [GA]^CCGG[CT]
HinfI 511 GACTC G^A[ACGT]TC
KspI 377 CCGCGG CCGC^GG
MaeI 578 CTAG C^TAG
NotI 130 GCGGCCGC GC^GGCCGC
PleI 511 GACTC GAGTC(4/5)
PpuMI 103 GGGACCC [GA]G^G[AT]CC[CT]
PstI 614 CTGCAG CTGCA^G
SacII 377 CCGCGG CCGC^GG
SapI 619 GAAGAGC GCTCTTC(1/4)
SfcI 614 CTGCAG C^T[GA][CT]AG
SfiI 372 GGCCGCCGCGGCC GGCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGCC
Tsp45I 491 GTGAC ^GT[GC]AC
Tsp509I 652 AATT ^AATT
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# TWO CUT ENZYMES ( 25 enzymes) #
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AlwNI 464 CAGCTCCTG CAG[ACGT][ACGT][ACGT]^CTG
608 CAGCCTCTG
ApaI 497 GGGCCC GGGCC^C
600 GGGCCC
AvaI 259 CCCGAG C^[CT]CG[GA]G
310 CCCGAG
BanI 306 GGCGCC G^G[CT][GA]CC
456 GGCGCC
BbeI 306 GGCGCC GGCGC^C
456 GGCGCC
BglI 244 GCCCCGAAGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
373 GCCGCCGCGGC
BlpI 12 GCTCAGC GC^T[ACGT]AGC
292 GCTGAGC
Bpu1102I 12 GCTCAGC GC^T[ACGT]AGC
292 GCTGAGC
BsaI 481 GAGACC GGTCTC(1/5)
553 GAGACC
BsiEI 131 CGGCCG CG[GA][CT]^CG
386 CGGCCG
BsmAI 481 GAGAC GTCTC(1/5)
553 GAGAC
BsoBI 259 CCCGAG C^[CT]CG[GA]G
310 CCCGAG
BstAPI 463 GCAGCTCCTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
607 GCAGCCTCTGC
CelII 12 GCTCAGC GC^T[ACGT]AGC
292 GCTGAGC
DsaI 377 CCGCGG C^C[GA][CT]GG
566 CCACGG
EaeI 131 CGGCCG [CT]^GGCC[GA]
386 CGGCCG
EagI 131 CGGCCG C^GGCCG
386 CGGCCG
EclXI 131 CGGCCG C^GGCCG
386 CGGCCG
Eco52I 131 CGGCCG C^GGCCG
386 CGGCCG
HgaI 181 GACGC GACGC(5/10)
220 GACGC
KasI 306 GGCGCC G^GCGCC
456 GGCGCC
MaeIII 491 GTGAC ^GT[ACGT]AC
667 GTAAC
MspA1I 338 CCGCTG C[AC]G^C[GT]G
377 CCGCGG
NarI 306 GGCGCC GG^CGCC
456 GGCGCC
SfoI 306 GGCGCC GGC^GCC
456 GGCGCC
#########################################
# 3 OR MORE CUT ENZYMES ( 49 enzymes) #
#########################################
AccII AciI AcyI AluI BanII
BbvI BcnI BmyI BsaHI BsaJI
BseRI BsiYI BslI Bsp1286I BssKI
BstNI BstUI Cac8I CfoI Cfr13I
DdeI DraII EarI EcoO109I EcoRII
EcoT14I FauI Fnu4HI HaeII HaeIII
HapII HhaI Hin1I HinP1I HpaII
ItaI Ksp632I MboII MnlI MspI
MvaI MvnI MwoI NciI NlaIV
Sau96I ScrFI StyI TseI