(Linear) (Six-base) MAPSORT of: KIEE2789.seq Check: 2836 from: 1 to: 681 KIEE2789.orf With 182 enzymes: SgfI * February 19, 2021 20:23 .. AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 0 491 681 Size: 491 190 AlwNI CAG_nnn'CTG Cuts at: 0 469 613 681 Size: 469 144 68 Fragments arranged by size: 469 144 68 ApaI G_GGCC'C Cuts at: 0 501 604 681 Size: 501 103 77 Fragments arranged by size: 501 103 77 AvaI C'yCGr_G Cuts at: 0 259 310 681 Size: 259 51 371 Fragments arranged by size: 371 259 51 BanI G'GyrC_C Cuts at: 0 306 456 681 Size: 306 150 225 Fragments arranged by size: 306 225 150 BanII G_rGCy'C Cuts at: 0 125 143 260 299 422 501 604 626 Size: 125 18 117 39 123 79 103 22 Cuts at: 626 681 Size: 55 Fragments arranged by size: 125 123 117 103 79 55 39 22 18 BbeI G_GCGC'C Cuts at: 0 310 460 681 Size: 310 150 221 Fragments arranged by size: 310 221 150 BbsI GAAGACnn'nnnn_ Cuts at: 0 271 681 Size: 271 410 BbvCI CC'TCA_GC Cuts at: 0 368 681 Size: 368 313 BglI GCCn_nnn'nGGC Cuts at: 0 250 379 681 Size: 250 129 302 Fragments arranged by size: 302 250 129 BlpI GC'TnA_GC Cuts at: 0 13 293 681 Size: 13 280 388 Fragments arranged by size: 388 280 13 Bme1580I G_kGCm'C Cuts at: 0 501 604 681 Size: 501 103 77 Fragments arranged by size: 501 103 77 BmrI ACTGGGnnnn_n' Cuts at: 0 461 681 Size: 461 220 BplI GAGnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 0 28 681 Size: 28 653 Bpu10I CC'TnA_GC Cuts at: 0 368 681 Size: 368 313 BsaI GGTCTCn'nnnn_ Cuts at: 0 475 547 681 Size: 475 72 134 Fragments arranged by size: 475 134 72 BsaHI Gr'CG_yC Cuts at: 0 182 307 457 681 Size: 182 125 150 224 Fragments arranged by size: 224 182 150 125 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 0 112 214 253 430 681 Size: 112 102 39 177 251 Fragments arranged by size: 251 177 112 102 39 BseYI C'CCAG_C Cuts at: 0 421 501 681 Size: 421 80 180 Fragments arranged by size: 421 180 80 BsiEI CG_ry'CG Cuts at: 0 134 389 681 Size: 134 255 292 Fragments arranged by size: 292 255 134 Bsp1286I G_dGCh'C Cuts at: 0 125 143 260 299 422 501 604 626 Size: 125 18 117 39 123 79 103 22 Cuts at: 626 681 Size: 55 Fragments arranged by size: 125 123 117 103 79 55 39 22 18 BsrFI r'CCGG_y Cuts at: 0 630 681 Size: 630 51 BstAPI GCAn_nnn'nTGC Cuts at: 0 469 613 681 Size: 469 144 68 Fragments arranged by size: 469 144 68 BstXI CCAn_nnnn'nTGG Cuts at: 0 633 681 Size: 633 48 BtgI C'CryG_G Cuts at: 0 377 566 681 Size: 377 189 115 Fragments arranged by size: 377 189 115 EaeI y'GGCC_r Cuts at: 0 131 386 681 Size: 131 255 295 Fragments arranged by size: 295 255 131 EagI C'GGCC_G Cuts at: 0 131 386 681 Size: 131 255 295 Fragments arranged by size: 295 255 131 EarI CTCTTCn'nnn_ Cuts at: 0 89 549 614 681 Size: 89 460 65 67 Fragments arranged by size: 460 89 67 65 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 0 268 681 Size: 268 413 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 0 491 681 Size: 491 190 EcoO109I rG'GnC_Cy Cuts at: 0 104 243 354 497 498 600 601 639 Size: 104 139 111 143 1 102 1 38 Cuts at: 639 681 Size: 42 Fragments arranged by size: 143 139 111 104 102 42 38 1 1 HaeII r_GCGC'y Cuts at: 0 310 460 577 681 Size: 310 150 117 104 Fragments arranged by size: 310 150 117 104 KasI G'GCGC_C Cuts at: 0 306 456 681 Size: 306 150 225 Fragments arranged by size: 306 225 150 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 0 680 681 Size: 680 1 MspA1I CmG'CkG Cuts at: 0 340 379 681 Size: 340 39 302 Fragments arranged by size: 340 302 39 NarI GG'CG_CC Cuts at: 0 307 457 681 Size: 307 150 224 Fragments arranged by size: 307 224 150 NotI GC'GGCC_GC Cuts at: 0 131 681 Size: 131 550 PasI CC'CwG_GG Cuts at: 0 597 681 Size: 597 84 PpuMI rG'GwC_Cy Cuts at: 0 104 681 Size: 104 577 PspOMI G'GGCC_C Cuts at: 0 497 600 681 Size: 497 103 81 Fragments arranged by size: 497 103 81 PstI C_TGCA'G Cuts at: 0 618 681 Size: 618 63 SacII CC_GC'GG Cuts at: 0 380 681 Size: 380 301 SanDI GG'GwC_CC Cuts at: 0 104 681 Size: 104 577 SapI GCTCTTCn'nnn_ Cuts at: 0 614 681 Size: 614 67 SfcI C'TryA_G Cuts at: 0 614 681 Size: 614 67 SfiI GGCCn_nnn'nGGCC Cuts at: 0 379 681 Size: 379 302 SfoI GGC'GCC Cuts at: 0 308 458 681 Size: 308 150 223 Fragments arranged by size: 308 223 150 StyI C'CwwG_G Cuts at: 0 56 152 233 287 548 590 681 Size: 56 96 81 54 261 42 91 Fragments arranged by size: 261 96 91 81 56 54 42 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 0 603 681 Size: 603 78 Enzymes that do cut: AcuI AlwNI ApaI AvaI BanI BanII BbeI BbsI BbvCI BglI BlpI Bme1580I BmrI BplI Bpu10I BsaI BsaHI BseRI BseYI BsiEI Bsp1286I BsrFI BstAPI BstXI BtgI EaeI EagI EarI EciI Eco57MI EcoO109I HaeII KasI MmeI MspA1I NarI NotI PasI PpuMI PspOMI PstI SacII SanDI SapI SfcI SfiI SfoI StyI TaqII Enzymes that do not cut: AarI AatII AccI Acc65I AclI AfeI AflII AflIII AgeI AhdI AleI AlfI AloI ApaLI ApoI AscI AseI AsiSI AvrII BaeI BamHI BcgI BciVI BclI BfrBI BglII BmgBI BmtI BpmI BpuEI BsaAI BsaBI BsaWI BsaXI BsgI BsiHKAI BsiWI BsmI BsmBI BspEI BspHI BspMI BsrBI BsrDI BsrGI BssHII BssSI BstBI BstEII BstYI BstZ17I Bsu36I BtgZI BtsI ClaI CspCI DraI DraIII DrdI EcoICRI EcoNI EcoRI EcoRV FalI FseI FspI FspAI Hin4I HincII HindIII HpaI KpnI MfeI MluI MscI MslI NaeI NcoI NdeI NgoMIV NheI NruI NsiI NspI PacI PciI PflMI PfoI PmeI PmlI PpiI PshAI PsiI PspXI PsrI PvuI PvuII RsrII SacI SalI SbfI ScaI SexAI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SrfI SspI StuI SwaI TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI