################################## # NO CUT ENZYMES ( 138 enzymes) # ################################## AatII Acc65I AccI AccII AclI AcsI AcyI AflII AgeI AhdI Alw44I AocI Aor51HI ApaI ApaLI ApoI AscI AseI AsnI Asp718I AspEI AspI AviII AvrII BamHI BbeI BbrPI BbvCI BciVI BclI BcnI BfrI BglI BglII BlnI BsaBI BsaHI BsaI BsiEI BsiWI BsmBI Bsp1407I BspDI BspMI BsrBI BsrFI BsrGI BssHII Bst1107I BstBI BstEII BstPI BstUI BstYI BstZ17I Bsu36I Cfr10I ClaI CpoI DraI DraII DrdI EagI Eam1105I EclXI Eco47III Eco52I Eco81I EcoNI EcoO109I EcoO65I EcoRI EcoRV FauI FbaI FseI FspI HgaI Hin1I HincII HindII HpaI KasI KpnI KspI MamI MfeI MflI MluI MunI MvnI NaeI NarI NciI NgoMIV NheI NotI NruI NspV PacI PflFI PflMI PinAI PmaCI PmeI PmlI PpuMI PshAI PshBI Psp1406I PstI PvuI RsrII SacII SalI SapI SbfI SexAI SfiI SfoI SfuI SgrAI SmaI SnaBI SpeI SphI Sse8387I SspBI SspI StuI SwaI Tsp45I Tth111I Van91I XbaI XhoII XmaCI XmaI ################################### # ONE CUT ENZYMES ( 43 enzymes) # ################################### AflIII 384 ACATGT A^C[GA][CT]GT AlwNI 486 CAGCTGCTG CAG[ACGT][ACGT][ACGT]^CTG Asp700I 1461 GAAAGATTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC AvaII 1309 GGACC G^G[AT]CC BalI 1250 TGGCCA TGG^CCA BanI 211 GGTGCC G^G[CT][GA]CC BanII 728 GAGCTC G[GA]GC[CT]^C BcgI 736 CGAGTACAGTGC (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10) BsaAI 115 TACGTG [CT]AC^GT[GA] BsmAI 541 GTCTC GTCTC(1/5) BsmI 1233 GAATGC GAATGC(1/-1) BspHI 1190 TCATGA T^CATGA BspLU11I 384 ACATGT A^CATGT BssSI 1168 CACGAG CACGAG(-5/-1) CfoI 633 GCGC GCG^C Cfr13I 1309 GGACC G^G[ACGT]CC DraIII 831 CACGAAGTG CAC[ACGT][ACGT][ACGT]^GTG DsaI 701 CCATGG C^C[GA][CT]GG EaeI 1250 TGGCCA [CT]^GGCC[GA] EcoT14I 701 CCATGG C^C[AT][AT]GG HaeII 632 GGCGCT [GA]GCGC^[CT] HhaI 633 GCGC GCG^C HinP1I 633 GCGC G^CGC HindIII 1131 AAGCTT A^AGCTT HphI 408 TCACC GGTGA(8/7) MaeII 116 ACGT A^CGT MaeIII 1025 GTTAC ^GT[ACGT]AC MluNI 1250 TGGCCA TGG^CCA MscI 1250 TGGCCA TGG^CCA MseI 287 TTAA T^TAA NcoI 701 CCATGG C^CATGG NdeI 1286 CATATG CA^TATG PaeR7I 359 CTCGAG C^TCGAG RcaI 1190 TCATGA T^CATGA SacI 728 GAGCTC GAGCT^C Sau96I 1309 GGACC G^G[ACGT]CC ScaI 1091 AGTACT AGT^ACT SfcI 1228 CTACAG C^T[GA][CT]AG StyI 701 CCATGG C^C[AT][AT]GG Tru9I 287 TTAA T^TAA XcmI 675 CCAGCTGTGCCCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG XhoI 359 CTCGAG C^TCGAG XmnI 1461 GAAAGATTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC ################################### # TWO CUT ENZYMES ( 24 enzymes) # ################################### AccIII 818 TCCGGA T^CCGGA 1306 TCCGGA AlwI 163 GATCC GGATC(4/5) 1211 GGATC AvaI 166 CCCGAG C^[CT]CG[GA]G 359 CTCGAG BlpI 236 GCTTAGC GC^T[ACGT]AGC 1342 GCTCAGC BpmI 673 CTCCAG CTGGAG(16/14) 1348 CTGGAG Bpu1102I 236 GCTTAGC GC^T[ACGT]AGC 1342 GCTCAGC BsaWI 818 TCCGGA [AT]^CCGG[AT] 1306 TCCGGA BseAI 818 TCCGGA T^CCGGA 1306 TCCGGA BseRI 169 GAGGAG GAGGAG(10/8) 1446 GAGGAG BsoBI 166 CCCGAG C^[CT]CG[GA]G 359 CTCGAG BspEI 818 TCCGGA T^CCGGA 1306 TCCGGA BstXI 128 CCAGCGATGTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG 1241 CCAAGCTCTTGG CelII 236 GCTTAGC GC^T[ACGT]AGC 1342 GCTCAGC EcoT22I 1289 ATGCAT ATGCA^T 1426 ATGCAT HaeIII 462 GGCC GG^CC 1251 GGCC HapII 819 CCGG C^CGG 1307 CCGG HpaII 819 CCGG C^CGG 1307 CCGG MroI 818 TCCGGA T^CCGGA 1306 TCCGGA MslI 409 CACCACTGTG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG 997 CATGGCAGTG MspI 819 CCGG C^CGG 1307 CCGG NsiI 1289 ATGCAT ATGCA^T 1426 ATGCAT NspI 384 ACATGT [GA]CATG^[CT] 935 ACATGC SfaNI 154 GATGC GCATC(5/9) 1425 GATGC SmlI 359 CTCGAG C^T[CT][GA]AG 1084 CTCAAG ######################################### # 3 OR MORE CUT ENZYMES ( 53 enzymes) # ######################################### AciI AfaI AluI AspHI BbsI BbvI BfaI BmyI BpuAI BsaJI BsgI BsiHKAI BsiYI BslI BsmFI Bsp1286I BsrDI BsrI BssKI BstAPI BstF5I BstNI Cac8I DdeI DpnII EarI EcoRII Fnu4HI FokI HinfI ItaI Ksp632I MaeI MboI MboII MnlI MspA1I MvaI MwoI NdeII NlaIII NlaIV PleI PvuII RsaI Sau3AI ScrFI TaqI TfiI TseI Tsp509I TspRI TthHB8I