(Circular) (Six-base) MAPSORT of: pFN21AE3670.seq Check: 9501 from: 1 to: 6184 pFN21AE3670 6184 bp With 182 enzymes: SgfI * December 28, 2011 15:26 .. AatII G_ACGT'C Cuts at: 278 331 414 600 1536 3994 278 Size: 53 83 186 936 2458 2468 Fragments arranged by size: 2468 2458 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 3527 1937 Size: 1590 4594 Acc65I G'GTAC_C Cuts at: 3505 3505 Size: 6184 AclI AA'CG_TT Cuts at: 1563 4311 4684 1563 Size: 2748 373 3063 Fragments arranged by size: 3063 2748 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 2632 4247 5631 5899 1632 Size: 1000 1615 1384 268 1917 Fragments arranged by size: 1917 1615 1384 1000 268 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 5987 AflIII A'CryG_T Cuts at: 1203 2389 5083 6060 1203 Size: 1186 2694 977 1327 Fragments arranged by size: 2694 1327 1186 977 AgeI A'CCGG_T Cuts at: 5015 5015 Size: 6184 AhdI GACnn_n'nnGTC Cuts at: 4913 4913 Size: 6184 AleI CACnn'nnGTG Cuts at: 1166 2418 1166 Size: 1252 4932 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 2957 1184 Size: 1773 4411 AloI GAACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 2168 2200 2168 Size: 32 6152 AlwNI CAG_nnn'CTG Cuts at: 2496 3927 5499 2496 Size: 1431 1572 3181 Fragments arranged by size: 3181 1572 1431 ApaLI G'TGCA_C Cuts at: 4241 5397 4241 Size: 1156 5028 ApoI r'AATT_y Cuts at: 1493 3493 3719 3871 5980 1493 Size: 2000 226 152 2109 1697 Fragments arranged by size: 2109 2000 1697 226 152 AseI AT'TA_AT Cuts at: 160 1032 4738 160 Size: 872 3706 1606 Fragments arranged by size: 3706 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 6184 AvaI C'yCGr_G Cuts at: 1870 1943 2171 2364 3509 1870 Size: 73 228 193 1145 4545 Fragments arranged by size: 4545 1145 228 193 73 BamHI G'GATC_C Cuts at: 3514 3881 3514 Size: 367 5817 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2216 3505 4960 618 Size: 325 217 627 429 1289 1455 1842 Fragments arranged by size: 1842 1455 1289 627 429 325 217 BanII G_rGCy'C Cuts at: 1391 2737 3503 1391 Size: 1346 766 4072 Fragments arranged by size: 4072 1346 766 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 2097 2761 3002 928 Size: 971 198 664 241 4110 Fragments arranged by size: 4110 971 664 241 198 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 2730 2764 4375 4409 1903 Size: 34 793 34 1611 34 3678 Fragments arranged by size: 3678 1611 793 34 34 34 BciVI GTATCCnnnnn_n' Cuts at: 4076 5286 4076 Size: 1210 4974 BclI T'GATC_A Cuts at: 1548 1548 Size: 6184 BfrBI ATG'CAT Cuts at: 3296 3433 3296 Size: 137 6047 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 4795 136 Size: 107 122 71 631 605 3123 1525 Fragments arranged by size: 3123 1525 631 605 122 107 71 BglII A'GATC_T Cuts at: 6179 6179 Size: 6184 BlpI GC'TnA_GC Cuts at: 2242 3348 3601 2242 Size: 1106 253 4825 Fragments arranged by size: 4825 1106 253 Bme1580I G_kGCm'C Cuts at: 1790 2221 2690 4245 5401 1790 Size: 431 469 1555 1156 2573 Fragments arranged by size: 2573 1555 1156 469 431 BmgBI CAC'GTC Cuts at: 1318 1318 Size: 6184 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 3915 4873 442 Size: 416 53 83 399 24 2498 958 1753 Fragments arranged by size: 2498 1753 958 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 6184 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2663 3374 4844 1239 Size: 1424 711 1470 2579 Fragments arranged by size: 2579 1470 1424 711 Bpu10I CC'TnA_GC Cuts at: 2559 2559 Size: 6184 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 3074 4309 5174 5472 3074 Size: 1235 865 298 3786 Fragments arranged by size: 3786 1235 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 4847 882 Size: 617 3348 2219 Fragments arranged by size: 3348 2219 617 BsaAI yAC'GTr Cuts at: 493 1204 2122 3975 493 Size: 711 918 1853 2702 Fragments arranged by size: 2702 1853 918 711 BsaBI GATnn'nnATC Cuts at: 6178 6178 Size: 6184 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 3991 4373 5008 275 Size: 53 83 186 936 2458 382 635 1451 Fragments arranged by size: 2458 1451 936 635 382 186 83 53 BsaWI w'CCGG_w Cuts at: 2823 3311 4617 5015 5289 5436 5847 2823 Size: 488 1306 398 274 147 411 3160 Fragments arranged by size: 3160 1306 488 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 2168 2198 1178 Size: 30 165 30 765 30 5164 Fragments arranged by size: 5164 765 165 30 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2189 3466 1186 Size: 1003 1277 3904 Fragments arranged by size: 3904 1277 1003 BseYI C'CCAG_C Cuts at: 2496 5387 5883 2496 Size: 2891 496 2797 Fragments arranged by size: 2891 2797 496 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 2442 2715 2865 3043 3414 1163 Size: 573 706 273 150 178 371 3933 Fragments arranged by size: 3933 706 573 371 273 178 150 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 3634 4395 5423 1540 Size: 275 186 1633 761 1028 2301 Fragments arranged by size: 2301 1633 1028 761 275 186 BsiHKAI G_wGCw'C Cuts at: 2232 2737 2881 4245 4330 5401 2232 Size: 505 144 1364 85 1071 3015 Fragments arranged by size: 3015 1364 1071 505 144 85 BsmI GAATG_Cn' Cuts at: 3244 3695 3788 3244 Size: 451 93 5640 Fragments arranged by size: 5640 451 93 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2221 2232 2690 2737 2881 3503 4245 Size: 399 431 11 458 47 144 622 742 Cuts at: 4245 4330 5401 1391 Size: 85 1071 2174 Fragments arranged by size: 2174 1071 742 622 458 431 399 144 85 47 11 BspEI T'CCGG_A Cuts at: 2823 3311 5847 2823 Size: 488 2536 3160 Fragments arranged by size: 3160 2536 488 BspHI T'CATG_A Cuts at: 3195 4073 5803 3195 Size: 878 1730 3576 Fragments arranged by size: 3576 1730 878 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 3539 844 Size: 2695 3489 BsrBI CCG'CTC Cuts at: 1394 1823 4071 1394 Size: 429 2248 3507 Fragments arranged by size: 3507 2248 429 BsrDI GCAATG_nn' Cuts at: 65 1248 2047 2600 2951 4679 4853 65 Size: 1183 799 553 351 1728 174 1396 Fragments arranged by size: 1728 1396 1183 799 553 351 174 BsrFI r'CCGG_y Cuts at: 1700 1810 4828 5015 1700 Size: 110 3018 187 2869 Fragments arranged by size: 3018 2869 187 110 BsrGI T'GTAC_A Cuts at: 96 96 Size: 6184 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 6184 BssSI C'ACGA_G Cuts at: 3173 4244 5256 3173 Size: 1071 1012 4101 Fragments arranged by size: 4101 1071 1012 BstAPI GCAn_nnn'nTGC Cuts at: 794 2440 2757 2910 794 Size: 1646 317 153 4068 Fragments arranged by size: 4068 1646 317 153 BstBI TT'CG_AA Cuts at: 4988 4988 Size: 6184 BstEII G'GTnAC_C Cuts at: 1184 1184 Size: 6184 BstXI CCAn_nnnn'nTGG Cuts at: 2011 2140 3253 2011 Size: 129 1113 4942 Fragments arranged by size: 4942 1113 129 BstYI r'GATC_y Cuts at: 1972 3514 3881 4266 4283 5735 5821 5833 6179 Size: 1542 367 385 17 1452 86 12 346 Cuts at: 6179 1972 Size: 1977 Fragments arranged by size: 1977 1542 1452 385 367 346 86 17 12 BtgI C'CryG_G Cuts at: 513 1065 2706 513 Size: 552 1641 3991 Fragments arranged by size: 3991 1641 552 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 2151 487 Size: 683 56 925 4520 Fragments arranged by size: 4520 925 683 56 BtsI GCAGTG_nn' Cuts at: 2430 2506 3013 3700 4494 4514 2430 Size: 76 507 687 794 20 4100 Fragments arranged by size: 4100 794 687 507 76 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 6184 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 6149 DraI TTT'AAA Cuts at: 3488 3488 Size: 6184 DraIII CAC_nnn'GTG Cuts at: 2841 2841 Size: 6184 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 5191 809 Size: 507 3875 1802 Fragments arranged by size: 3875 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 3255 3631 4520 Size: 54 1425 50 275 18 1425 376 889 Cuts at: 4520 6149 8 Size: 1629 43 Fragments arranged by size: 1629 1425 1425 889 376 275 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 3631 1537 Size: 275 1819 4090 Fragments arranged by size: 4090 1819 275 EarI CTCTTCn'nnn_ Cuts at: 1353 2040 2067 2651 4114 1353 Size: 687 27 584 1463 3423 Fragments arranged by size: 3423 1463 687 584 27 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 4771 5145 5291 387 Size: 1064 138 3182 374 146 1280 Fragments arranged by size: 3182 1280 1064 374 146 138 EcoICRI GAG'CTC Cuts at: 2735 2735 Size: 6184 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2632 2663 3374 4247 4844 5631 5899 Size: 393 1000 31 711 873 597 787 268 Cuts at: 5899 1239 Size: 1524 Fragments arranged by size: 1524 1000 873 787 711 597 393 268 31 EcoRI G'AATT_C Cuts at: 3493 3871 3493 Size: 378 5806 FalI AAGnnnnnCTTnnnnnnnn_nnnnn' Cuts at: 3115 3115 Size: 6184 FspI TGC'GCA Cuts at: 4690 4690 Size: 6184 HaeII r_GCGC'y Cuts at: 1678 2641 5331 1678 Size: 963 2690 2531 Fragments arranged by size: 2690 2531 963 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 4825 4857 4899 4931 1373 Size: 32 135 32 3253 32 42 32 2626 Fragments arranged by size: 3253 2626 135 42 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 3528 3774 669 Size: 988 281 1590 246 3079 Fragments arranged by size: 3079 1590 988 281 246 HindIII A'AGCT_T Cuts at: 748 3136 748 Size: 2388 3796 HpaI GTT'AAC Cuts at: 3774 3774 Size: 6184 KpnI G_GTAC'C Cuts at: 3509 3509 Size: 6184 MfeI C'AATT_G Cuts at: 3783 3783 Size: 6184 MluI A'CGCG_T Cuts at: 6060 6060 Size: 6184 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 5298 5482 1305 Size: 3993 184 2007 Fragments arranged by size: 3993 2007 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 3257 10 Size: 54 1425 343 1425 2937 Fragments arranged by size: 2937 1425 1425 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 2009 2418 3006 4142 4501 4660 518 Size: 648 843 409 588 1136 359 159 2042 Fragments arranged by size: 2042 1136 843 648 588 409 359 159 MspA1I CmG'CkG Cuts at: 1606 2493 2683 3166 3352 3448 3600 4277 5425 Size: 887 190 483 186 96 152 677 1148 Cuts at: 5425 5670 1606 Size: 245 2120 Fragments arranged by size: 2120 1148 887 677 483 245 190 186 152 96 NcoI C'CATG_G Cuts at: 513 1065 2706 513 Size: 552 1641 3991 Fragments arranged by size: 3991 1641 552 NdeI CA'TA_TG Cuts at: 387 3292 387 Size: 2905 3279 NheI G'CTAG_C Cuts at: 1052 1052 Size: 6184 NotI GC'GGCC_GC Cuts at: 3631 3631 Size: 6184 NsiI A_TGCA'T Cuts at: 3298 3435 3298 Size: 137 6047 NspI r_CATG'y Cuts at: 1132 1229 2393 2944 3542 5087 1132 Size: 97 1164 551 598 1545 2229 Fragments arranged by size: 2229 1545 1164 598 551 97 PasI CC'CwG_GG Cuts at: 1348 2099 1348 Size: 751 5433 PciI A'CATG_T Cuts at: 2389 5083 2389 Size: 2694 3490 PflMI CCAn_nnn'nTGG Cuts at: 1108 1108 Size: 6184 PmeI GTTT'AAAC Cuts at: 3488 3488 Size: 6184 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 2168 2200 4253 4285 5766 5798 2168 Size: 32 2053 32 1481 32 2554 Fragments arranged by size: 2554 2053 1481 32 32 32 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 6184 PsiI TTA'TAA Cuts at: 3754 3754 Size: 6184 PspXI vC'TCGA_Gb Cuts at: 2364 2364 Size: 6184 PstI C_TGCA'G Cuts at: 830 3536 830 Size: 2706 3478 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 6184 PvuII CAG'CTG Cuts at: 2493 2683 3352 2493 Size: 190 669 5325 Fragments arranged by size: 5325 669 190 SacI G_AGCT'C Cuts at: 2737 2737 Size: 6184 SalI G'TCGA_C Cuts at: 1936 3526 1936 Size: 1590 4594 SbfI CC_TGCA'GG Cuts at: 3536 3536 Size: 6184 ScaI AGT'ACT Cuts at: 3098 4432 6144 3098 Size: 1334 1712 3138 Fragments arranged by size: 3138 1712 1334 SfcI C'TryA_G Cuts at: 826 1045 3233 3532 4667 5348 5539 826 Size: 219 2188 299 1135 681 191 1471 Fragments arranged by size: 2188 1471 1135 681 299 219 191 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 6184 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 6184 SmaI CCC'GGG Cuts at: 1872 3511 1872 Size: 1639 4545 SmlI C'TyrA_G Cuts at: 820 1017 1943 2364 3089 4288 5189 5451 820 Size: 197 926 421 725 1199 901 262 1553 Fragments arranged by size: 1553 1199 926 901 725 421 262 197 SnaBI TAC'GTA Cuts at: 493 3975 493 Size: 3482 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 6184 SphI G_CATG'C Cuts at: 1132 3542 1132 Size: 2410 3774 SspI AAT'ATT Cuts at: 5 52 4108 5 Size: 47 4056 2081 Fragments arranged by size: 4056 2081 47 StuI AGG'CCT Cuts at: 1517 1517 Size: 6184 StyI C'CwwG_G Cuts at: 513 1065 2706 3623 513 Size: 552 1641 917 3074 Fragments arranged by size: 3074 1641 917 552 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 2818 4211 4228 4381 4566 5011 325 Size: 1255 1238 1393 17 153 185 445 1498 Fragments arranged by size: 1498 1393 1255 1238 445 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 2743 3096 4430 Size: 274 80 33 51 1443 766 353 1334 Cuts at: 4430 6142 96 Size: 1712 138 Fragments arranged by size: 1712 1443 1334 766 353 274 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 2389 2421 6144 6176 2389 Size: 32 3723 32 2397 Fragments arranged by size: 3723 2397 32 32 Tth111I GACn'n_nGTC Cuts at: 1316 1316 Size: 6184 XbaI T'CTAG_A Cuts at: 3520 3520 Size: 6184 XcmI CCAnnnn_n'nnnnTGG Cuts at: 2687 2687 Size: 6184 XhoI C'TCGA_G Cuts at: 1943 2364 1943 Size: 421 5763 XmaI C'CCGG_G Cuts at: 1870 3509 1870 Size: 1639 4545 XmnI GAAnn'nnTTC Cuts at: 3470 4313 3470 Size: 843 5341 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 3992 276 Size: 53 83 186 936 2458 2468 Fragments arranged by size: 2468 2458 936 186 83 53 Enzymes that do cut: AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AloI AlwNI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbsI BcgI BciVI BclI BfrBI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI BtgI BtgZI BtsI ClaI CspCI DraI DraIII DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoRI FalI FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I NcoI NdeI NheI NotI NsiI NspI PasI PciI PflMI PmeI PpiI PshAI PsiI PspXI PstI PvuI PvuII SacI SalI SbfI ScaI SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI Enzymes that do not cut: AarI AfeI ApaI AscI AvrII BaeI BbeI BbvCI BplI BsiWI BsmBI BstZ17I Bsu36I EcoNI EcoO109I EcoRV FseI FspAI KasI NaeI NarI NgoMIV NruI PacI PfoI PmlI PpuMI PspOMI PsrI RsrII SacII SanDI SapI SexAI SfiI SfoI SrfI SwaI