(Linear) (Six-base) MAPSORT of: KIEE3670.seq Check: 4070 from: 1 to: 1479 KIEE3670.orf With 182 enzymes: SgfI * December 27, 2011 16:38 .. AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 0 627 1479 Size: 627 852 AflIII A'CryG_T Cuts at: 0 384 1479 Size: 384 1095 AleI CACnn'nnGTG Cuts at: 0 413 1479 Size: 413 1066 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 0 952 1479 Size: 952 527 AloI GAACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 0 163 195 1479 Size: 163 32 1284 Fragments arranged by size: 1284 163 32 AlwNI CAG_nnn'CTG Cuts at: 0 491 1479 Size: 491 988 AvaI C'yCGr_G Cuts at: 0 166 359 1479 Size: 166 193 1120 Fragments arranged by size: 1120 193 166 BanI G'GyrC_C Cuts at: 0 211 1479 Size: 211 1268 BanII G_rGCy'C Cuts at: 0 732 1479 Size: 732 747 BbsI GAAGACnn'nnnn_ Cuts at: 0 92 756 997 1479 Size: 92 664 241 482 Fragments arranged by size: 664 482 241 92 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 0 725 759 1479 Size: 725 34 720 Fragments arranged by size: 725 720 34 BfrBI ATG'CAT Cuts at: 0 1291 1428 1479 Size: 1291 137 51 Fragments arranged by size: 1291 137 51 BlpI GC'TnA_GC Cuts at: 0 237 1343 1479 Size: 237 1106 136 Fragments arranged by size: 1106 237 136 Bme1580I G_kGCm'C Cuts at: 0 216 685 1479 Size: 216 469 794 Fragments arranged by size: 794 469 216 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 0 658 1369 1479 Size: 658 711 110 Fragments arranged by size: 711 658 110 Bpu10I CC'TnA_GC Cuts at: 0 554 1479 Size: 554 925 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 0 1069 1479 Size: 1069 410 BsaAI yAC'GTr Cuts at: 0 117 1479 Size: 117 1362 BsaWI w'CCGG_w Cuts at: 0 818 1306 1479 Size: 818 488 173 Fragments arranged by size: 818 488 173 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 0 163 193 1479 Size: 163 30 1286 Fragments arranged by size: 1286 163 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 0 184 1461 1479 Size: 184 1277 18 Fragments arranged by size: 1277 184 18 BseYI C'CCAG_C Cuts at: 0 491 1479 Size: 491 988 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 0 437 710 860 1038 1409 1479 Size: 437 273 150 178 371 70 Fragments arranged by size: 437 371 273 178 150 70 BsiHKAI G_wGCw'C Cuts at: 0 227 732 876 1479 Size: 227 505 144 603 Fragments arranged by size: 603 505 227 144 BsmI GAATG_Cn' Cuts at: 0 1239 1479 Size: 1239 240 Bsp1286I G_dGCh'C Cuts at: 0 216 227 685 732 876 1479 Size: 216 11 458 47 144 603 Fragments arranged by size: 603 458 216 144 47 11 BspEI T'CCGG_A Cuts at: 0 818 1306 1479 Size: 818 488 173 Fragments arranged by size: 818 488 173 BspHI T'CATG_A Cuts at: 0 1190 1479 Size: 1190 289 BsrDI GCAATG_nn' Cuts at: 0 42 595 946 1479 Size: 42 553 351 533 Fragments arranged by size: 553 533 351 42 BssSI C'ACGA_G Cuts at: 0 1168 1479 Size: 1168 311 BstAPI GCAn_nnn'nTGC Cuts at: 0 435 752 905 1479 Size: 435 317 153 574 Fragments arranged by size: 574 435 317 153 BstXI CCAn_nnnn'nTGG Cuts at: 0 135 1248 1479 Size: 135 1113 231 Fragments arranged by size: 1113 231 135 BtgI C'CryG_G Cuts at: 0 701 1479 Size: 701 778 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 0 146 1479 Size: 146 1333 BtsI GCAGTG_nn' Cuts at: 0 425 501 1008 1479 Size: 425 76 507 471 Fragments arranged by size: 507 471 425 76 DraIII CAC_nnn'GTG Cuts at: 0 836 1479 Size: 836 643 EaeI y'GGCC_r Cuts at: 0 1250 1479 Size: 1250 229 EarI CTCTTCn'nnn_ Cuts at: 0 35 62 646 1479 Size: 35 27 584 833 Fragments arranged by size: 833 584 35 27 EcoICRI GAG'CTC Cuts at: 0 730 1479 Size: 730 749 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 0 627 658 1369 1479 Size: 627 31 711 110 Fragments arranged by size: 711 627 110 31 FalI AAGnnnnnCTTnnnnnnnn_nnnnn' Cuts at: 0 1110 1479 Size: 1110 369 HaeII r_GCGC'y Cuts at: 0 636 1479 Size: 636 843 HindIII A'AGCT_T Cuts at: 0 1131 1479 Size: 1131 348 MscI TGG'CCA Cuts at: 0 1252 1479 Size: 1252 227 MslI CAynn'nnrTG Cuts at: 0 413 1001 1479 Size: 413 588 478 Fragments arranged by size: 588 478 413 MspA1I CmG'CkG Cuts at: 0 488 678 1161 1347 1443 1479 Size: 488 190 483 186 96 36 Fragments arranged by size: 488 483 190 186 96 36 NcoI C'CATG_G Cuts at: 0 701 1479 Size: 701 778 NdeI CA'TA_TG Cuts at: 0 1287 1479 Size: 1287 192 NsiI A_TGCA'T Cuts at: 0 1293 1430 1479 Size: 1293 137 49 Fragments arranged by size: 1293 137 49 NspI r_CATG'y Cuts at: 0 388 939 1479 Size: 388 551 540 Fragments arranged by size: 551 540 388 PasI CC'CwG_GG Cuts at: 0 94 1479 Size: 94 1385 PciI A'CATG_T Cuts at: 0 384 1479 Size: 384 1095 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 0 163 195 1479 Size: 163 32 1284 Fragments arranged by size: 1284 163 32 PspXI vC'TCGA_Gb Cuts at: 0 359 1479 Size: 359 1120 PvuII CAG'CTG Cuts at: 0 488 678 1347 1479 Size: 488 190 669 132 Fragments arranged by size: 669 488 190 132 SacI G_AGCT'C Cuts at: 0 732 1479 Size: 732 747 ScaI AGT'ACT Cuts at: 0 1093 1479 Size: 1093 386 SfcI C'TryA_G Cuts at: 0 1228 1479 Size: 1228 251 SmlI C'TyrA_G Cuts at: 0 359 1084 1479 Size: 359 725 395 Fragments arranged by size: 725 395 359 StyI C'CwwG_G Cuts at: 0 701 1479 Size: 701 778 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 0 813 1479 Size: 813 666 TatI w'GTAC_w Cuts at: 0 738 1091 1479 Size: 738 353 388 Fragments arranged by size: 738 388 353 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 0 384 416 1479 Size: 384 32 1063 Fragments arranged by size: 1063 384 32 XcmI CCAnnnn_n'nnnnTGG Cuts at: 0 682 1479 Size: 682 797 XhoI C'TCGA_G Cuts at: 0 359 1479 Size: 359 1120 XmnI GAAnn'nnTTC Cuts at: 0 1465 1479 Size: 1465 14 Enzymes that do cut: AcuI AflIII AleI AlfI AloI AlwNI AvaI BanI BanII BbsI BcgI BfrBI BlpI Bme1580I BpmI Bpu10I BpuEI BsaAI BsaWI BsaXI BseRI BseYI BsgI BsiHKAI BsmI Bsp1286I BspEI BspHI BsrDI BssSI BstAPI BstXI BtgI BtgZI BtsI DraIII EaeI EarI EcoICRI Eco57MI FalI HaeII HindIII MscI MslI MspA1I NcoI NdeI NsiI NspI PasI PciI PpiI PspXI PvuII SacI ScaI SfcI SmlI StyI TaqII TatI TstI XcmI XhoI XmnI Enzymes that do not cut: AarI AatII AccI Acc65I AclI AfeI AflII AgeI AhdI ApaI ApaLI ApoI AscI AseI AsiSI AvrII BaeI BamHI BbeI BbvCI BciVI BclI BglI BglII BmgBI BmrI BmtI BplI BsaI BsaBI BsaHI BsiEI BsiWI BsmBI BspMI BsrBI BsrFI BsrGI BssHII BstBI BstEII BstYI BstZ17I Bsu36I ClaI CspCI DraI DrdI EagI EciI EcoNI EcoO109I EcoRI EcoRV FseI FspI FspAI Hin4I HincII HpaI KasI KpnI MfeI MluI MmeI NaeI NarI NgoMIV NheI NotI NruI PacI PflMI PfoI PmeI PmlI PpuMI PshAI PsiI PspOMI PsrI PstI PvuI RsrII SacII SalI SanDI SapI SbfI SexAI SfiI SfoI SgfI SgrAI SmaI SnaBI SpeI SphI SrfI SspI StuI SwaI Tth111I XbaI XmaI ZraI