################################## # NO CUT ENZYMES ( 97 enzymes) # ################################## Acc65I AccIII AclI AcsI AflII AflIII AgeI AhdI ApoI AscI AseI AsnI Asp718I AspEI AspI AvrII BamHI BbeI BbrPI BcgI BciVI BfrI BglII BlnI BseAI BsiEI BsiWI BsmBI Bsp1407I BspDI BspEI BspLU11I BsrBI BsrGI BssHII BssSI BstBI ClaI CpoI DraI DrdI EagI Eam1105I EclXI Eco52I EcoRI EcoRV EcoT22I FseI HgaI HincII HindII HindIII HpaI KasI KpnI KspI MluI MroI MseI NarI NdeI NheI NotI NruI NsiI NspI NspV PacI PaeR7I PflFI PinAI PmaCI PmeI PmlI PshBI Psp1406I PvuI RsrII SacII SalI SapI ScaI SfoI SfuI SgrAI SnaBI SphI SspBI SspI StuI SwaI Tru9I Tth111I XbaI XcmI XhoI ################################### # ONE CUT ENZYMES ( 58 enzymes) # ################################### AatII 547 GACGTC GACGT^C AccII 72 CGCG CG^CG AcyI 547 GACGTC G[GA]^CG[CT]C Alw44I 136 GTGCAC G^TGCAC AocI 1981 CCTCAGG CC^T[ACGT]AGG Aor51HI 408 AGCGCT AGC^GCT ApaLI 136 GTGCAC G^TGCAC Asp700I 351 GAAGCAGTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC AviII 317 TGCGCA TGC^GCA BalI 1629 TGGCCA TGG^CCA BanI 298 GGCACC G^G[CT][GA]CC BbvCI 1932 CCTCAGC CCTCAGC(-5/-2) BclI 1838 TGATCA T^GATCA BfaI 1600 CTAG C^TAG BsaAI 850 TACGTG [CT]AC^GT[GA] BsaBI 1839 GATCAACATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC BsaHI 547 GACGTC G[GA]^CG[CT]C BsgI 1429 GTGCAG GTGCAG(16/14) BsmI 2160 GAATGC GAATGC(1/-1) BspHI 2156 TCATGA T^CATGA BsrDI 1658 GCAATG GCAATG(2/0) Bst1107I 1008 GTATAC GTA^TAC BstUI 72 CGCG CG^CG BstYI 1073 GGATCT [GA]^GATC[CT] BstZ17I 1008 GTATAC GTA^TAC Bsu36I 1981 CCTCAGG CC^T[ACGT]AGG DraIII 141 CACTGAGTG CAC[ACGT][ACGT][ACGT]^GTG Eco47III 408 AGCGCT AGC^GCT Eco81I 1981 CCTCAGG CC^T[ACGT]AGG FauI 70 CCCGC CCCGC(4/6) FbaI 1838 TGATCA T^GATCA FspI 317 TGCGCA TGC^GCA Hin1I 547 GACGTC G[GA]^CG[CT]C MaeI 1600 CTAG C^TAG MamI 1839 GATCAACATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC MfeI 1241 CAATTG C^AATTG MflI 1073 GGATCT [GA]^GATC[CT] MluNI 1629 TGGCCA TGG^CCA MscI 1629 TGGCCA TGG^CCA MunI 1241 CAATTG C^AATTG MvnI 72 CGCG CG^CG NaeI 1151 GCCGGC GCC^GGC NgoMIV 1151 GCCGGC G^CCGGC PshAI 928 GACCAATGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC RcaI 2156 TCATGA T^CATGA SacI 877 GAGCTC GAGCT^C SbfI 241 CCTGCAGG CCTGCA^GG SexAI 200 ACCTGGT A^CC[AT]GGT SfiI 1815 GGCCTGCGTGGCC GGCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGCC SmaI 263 CCCGGG CCC^GGG SpeI 1599 ACTAGT A^CTAGT Sse8387I 241 CCTGCAGG CCTGCA^GG TfiI 185 GATTC G^A[AT]TC Tsp509I 1242 AATT ^AATT XhoII 1073 GGATCT [GA]^GATC[CT] XmaCI 263 CCCGGG C^CCGGG XmaI 263 CCCGGG C^CCGGG XmnI 351 GAAGCAGTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC ################################### # TWO CUT ENZYMES ( 23 enzymes) # ################################### AccI 943 GTCTAC GT^[AC][GT]AC 1008 GTATAC AvaI 28 CCCGAG C^[CT]CG[GA]G 263 CCCGGG BbsI 628 GAAGAC GAAGAC(2/6) 1989 GAAGAC BglI 1816 GCCTGCGTGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC 2085 GCCTGTTCGGC BpuAI 628 GAAGAC GAAGAC(2/6) 1989 GAAGAC BsaI 259 GAGACC GGTCTC(1/5) 1804 GAGACC BsoBI 28 CCCGAG C^[CT]CG[GA]G 263 CCCGGG BsrFI 1151 GCCGGC [GA]^CCGG[CT] 2024 ACCGGC BstAPI 235 GCAGGACCTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC 1913 GCAGTGACTGC BstEII 102 GGTCACC G^GT[ACGT]ACC 1017 GGTTACC BstPI 102 GGTCACC G^GT[ACGT]ACC 1017 GGTTACC BstXI 536 CCAAAGCCTTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG 1782 CCACTTGATTGG Cfr10I 1151 GCCGGC [GA]^CCGG[CT] 2024 ACCGGC EaeI 120 CGGCCA [CT]^GGCC[GA] 1629 TGGCCA EarI 906 CTCTTC CTCTTC(1/4) 346 GAAGAG EcoNI 1315 CCTTCTGCAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG 1353 CCTGGAGGAGG EcoO65I 102 GGTCACC G^GT[ACGT]ACC 1017 GGTTACC Ksp632I 906 CTCTTC CTCTTC(1/4) 346 GAAGAG NcoI 1502 CCATGG C^CATGG 1761 CCATGG PflMI 195 CCAATACCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG 1119 CCAGAGAGTGG TaqI 183 TCGA T^CGA 686 TCGA TthHB8I 183 TCGA T^CGA 686 TCGA Van91I 195 CCAATACCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG 1119 CCAGAGAGTGG ######################################### # 3 OR MORE CUT ENZYMES ( 80 enzymes) # ######################################### AciI AfaI AluI AlwI AlwNI ApaI AspHI AvaII BanII BbvI BcnI BlpI BmyI BpmI Bpu1102I BsaJI BsaWI BseRI BsiHKAI BsiYI BslI BsmAI BsmFI Bsp1286I BspMI BsrI BssKI BstF5I BstNI Cac8I CelII CfoI Cfr13I DdeI DpnII DraII DsaI EcoO109I EcoRII EcoT14I Fnu4HI FokI HaeII HaeIII HapII HhaI HinP1I HinfI HpaII HphI ItaI MaeII MaeIII MboI MboII MnlI MslI MspA1I MspI MvaI MwoI NciI NdeII NlaIII NlaIV PleI PpuMI PstI PvuII RsaI Sau3AI Sau96I ScrFI SfaNI SfcI SmlI StyI TseI Tsp45I TspRI