(Circular) (Six-base) MAPSORT of: pFN21AE5712.seq Check: 1952 from: 1 to: 6901 pFN21AE5712 6901 bp With 182 enzymes: SgfI * December 8, 2014 13:42 .. AatII G_ACGT'C Cuts at: 278 331 414 600 1536 2556 4711 278 Size: 53 83 186 936 1020 2155 2468 Fragments arranged by size: 2468 2155 1020 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 2949 3014 4244 1937 Size: 1012 65 1230 4594 Fragments arranged by size: 4594 1230 1012 65 Acc65I G'GTAC_C Cuts at: 4222 4222 Size: 6901 AclI AA'CG_TT Cuts at: 1563 5028 5401 1563 Size: 3465 373 3063 Fragments arranged by size: 3465 3063 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 2283 2606 4964 6348 6616 1632 Size: 651 323 2358 1384 268 1917 Fragments arranged by size: 2358 1917 1384 651 323 268 AfeI AGC'GCT Cuts at: 2415 2415 Size: 6901 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 6704 AflIII A'CryG_T Cuts at: 1203 5800 6777 1203 Size: 4597 977 1327 Fragments arranged by size: 4597 1327 977 AgeI A'CCGG_T Cuts at: 5732 5732 Size: 6901 AhdI GACnn_n'nnGTC Cuts at: 5630 5630 Size: 6901 AleI CACnn'nnGTG Cuts at: 1166 3185 1166 Size: 2019 4882 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 2687 3110 3419 1184 Size: 1503 423 309 4666 Fragments arranged by size: 4666 1503 423 309 AloI GAACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 3699 3731 3699 Size: 32 6869 AlwNI CAG_nnn'CTG Cuts at: 2020 2246 2365 3166 3822 3924 4644 6216 2020 Size: 226 119 801 656 102 720 1572 2705 Fragments arranged by size: 2705 1572 801 720 656 226 119 102 ApaI G_GGCC'C Cuts at: 2102 2345 2761 3534 2102 Size: 243 416 773 5469 Fragments arranged by size: 5469 773 416 243 ApaLI G'TGCA_C Cuts at: 2141 4958 6114 2141 Size: 2817 1156 2928 Fragments arranged by size: 2928 2817 1156 ApoI r'AATT_y Cuts at: 1493 4210 4436 4588 6697 1493 Size: 2717 226 152 2109 1697 Fragments arranged by size: 2717 2109 1697 226 152 AseI AT'TA_AT Cuts at: 160 1032 5455 160 Size: 872 4423 1606 Fragments arranged by size: 4423 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 6901 AvaI C'yCGr_G Cuts at: 1870 1943 2033 2268 4226 1870 Size: 73 90 235 1958 4545 Fragments arranged by size: 4545 1958 235 90 73 BamHI G'GATC_C Cuts at: 4231 4598 4231 Size: 367 6534 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2303 4222 5677 618 Size: 325 217 627 516 1919 1455 1842 Fragments arranged by size: 1919 1842 1455 627 516 325 217 BanII G_rGCy'C Cuts at: 1391 2034 2102 2345 2761 2805 2886 2930 3375 Size: 643 68 243 416 44 81 44 445 Cuts at: 3375 3534 3869 4220 1391 Size: 159 335 351 4072 Fragments arranged by size: 4072 643 445 416 351 335 243 159 81 68 44 44 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 2640 4001 928 Size: 971 741 1361 3828 Fragments arranged by size: 3828 1361 971 741 BbvCI CC'TCA_GC Cuts at: 3938 3938 Size: 6901 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 5092 5126 1903 Size: 34 3155 34 3678 Fragments arranged by size: 3678 3155 34 34 BciVI GTATCCnnnnn_n' Cuts at: 4793 6003 4793 Size: 1210 5691 BclI T'GATC_A Cuts at: 1548 3843 1548 Size: 2295 4606 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 3827 4096 5512 Size: 107 122 71 631 605 2155 269 1416 Cuts at: 5512 136 Size: 1525 Fragments arranged by size: 2155 1525 1416 631 605 269 122 107 71 BglII A'GATC_T Cuts at: 6896 6896 Size: 6901 BlpI GC'TnA_GC Cuts at: 2024 2081 2282 2924 3147 4318 2024 Size: 57 201 642 223 1171 4607 Fragments arranged by size: 4607 1171 642 223 201 57 Bme1580I G_kGCm'C Cuts at: 1790 2102 2145 2345 2761 3194 3507 3534 4041 Size: 312 43 200 416 433 313 27 507 Cuts at: 4041 4072 4962 6118 1790 Size: 31 890 1156 2573 Fragments arranged by size: 2573 1156 890 507 433 416 313 312 200 43 31 27 BmgBI CAC'GTC Cuts at: 1318 1318 Size: 6901 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 2647 3400 4632 Size: 416 53 83 399 24 1230 753 1232 Cuts at: 4632 5590 442 Size: 958 1753 Fragments arranged by size: 1753 1232 1230 958 753 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 6901 BplI GAGnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 2905 3088 2905 Size: 183 6718 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2352 2387 2438 2784 3234 3380 3471 3701 Size: 1113 35 51 346 450 146 91 230 Cuts at: 3701 5561 1239 Size: 1860 2579 Fragments arranged by size: 2579 1860 1113 450 346 230 146 91 51 35 Bpu10I CC'TnA_GC Cuts at: 2063 3938 2063 Size: 1875 5026 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 2378 2735 4058 5026 5891 6189 2378 Size: 357 1323 968 865 298 3090 Fragments arranged by size: 3090 1323 968 865 357 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 2258 3803 5564 882 Size: 617 759 1545 1761 2219 Fragments arranged by size: 2219 1761 1545 759 617 BsaAI yAC'GTr Cuts at: 493 1204 2857 4692 493 Size: 711 1653 1835 2702 Fragments arranged by size: 2702 1835 1653 711 BsaBI GATnn'nnATC Cuts at: 3848 6895 3848 Size: 3047 3854 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 2553 4708 5090 5725 Size: 53 83 186 936 1020 2155 382 635 Cuts at: 5725 275 Size: 1451 Fragments arranged by size: 2155 1451 1020 936 635 382 186 83 53 BsaWI w'CCGG_w Cuts at: 2133 2385 2808 5334 5732 6006 6153 6564 2133 Size: 252 423 2526 398 274 147 411 2470 Fragments arranged by size: 2526 2470 423 411 398 274 252 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 3699 3729 3839 3869 4133 Size: 30 165 30 2296 30 110 30 264 Cuts at: 4133 4163 1178 Size: 30 3916 Fragments arranged by size: 3916 2296 264 165 110 30 30 30 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2051 2876 3377 1186 Size: 865 825 501 4710 Fragments arranged by size: 4710 865 825 501 BseYI C'CCAG_C Cuts at: 3058 3284 3568 6104 6600 3058 Size: 226 284 2536 496 3359 Fragments arranged by size: 3359 2536 496 284 226 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 3455 1163 Size: 573 1719 4609 Fragments arranged by size: 4609 1719 573 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 4351 5112 6140 1540 Size: 275 186 2350 761 1028 2301 Fragments arranged by size: 2350 2301 1028 761 275 186 BsiHKAI G_wGCw'C Cuts at: 2145 2676 2886 3717 4176 4962 5047 6118 2145 Size: 531 210 831 459 786 85 1071 2928 Fragments arranged by size: 2928 1071 831 786 531 459 210 85 BsmI GAATG_Cn' Cuts at: 4171 4412 4505 4171 Size: 241 93 6567 Fragments arranged by size: 6567 241 93 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2034 2102 2145 2345 2676 2761 2805 Size: 399 244 68 43 200 331 85 44 Cuts at: 2805 2886 2930 3194 3375 3507 3534 3717 3869 Size: 81 44 264 181 132 27 183 152 Cuts at: 3869 4041 4072 4176 4220 4962 5047 6118 1391 Size: 172 31 104 44 742 85 1071 2174 Fragments arranged by size: 2174 1071 742 399 331 264 244 200 183 181 172 152 132 104 85 85 81 68 44 44 44 43 31 27 BspEI T'CCGG_A Cuts at: 6564 6564 Size: 6901 BspHI T'CATG_A Cuts at: 4161 4790 6520 4161 Size: 629 1730 4542 Fragments arranged by size: 4542 1730 629 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 2254 2829 2961 4256 844 Size: 1410 575 132 1295 3489 Fragments arranged by size: 3489 1410 1295 575 132 BsrBI CCG'CTC Cuts at: 1394 1823 4788 1394 Size: 429 2965 3507 Fragments arranged by size: 3507 2965 429 BsrDI GCAATG_nn' Cuts at: 65 1248 3670 5396 5570 65 Size: 1183 2422 1726 174 1396 Fragments arranged by size: 2422 1726 1396 1183 174 BsrFI r'CCGG_y Cuts at: 1700 1810 3156 4029 5545 5732 1700 Size: 110 1346 873 1516 187 2869 Fragments arranged by size: 2869 1516 1346 873 187 110 BsrGI T'GTAC_A Cuts at: 96 96 Size: 6901 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 6901 BssSI C'ACGA_G Cuts at: 4961 5973 4961 Size: 1012 5889 BstAPI GCAn_nnn'nTGC Cuts at: 794 2246 3924 794 Size: 1452 1678 3771 Fragments arranged by size: 3771 1678 1452 BstBI TT'CG_AA Cuts at: 5705 5705 Size: 6901 BstEII G'GTnAC_C Cuts at: 1184 2107 3022 1184 Size: 923 915 5063 Fragments arranged by size: 5063 923 915 BstXI CCAn_nnnn'nTGG Cuts at: 2548 3794 2548 Size: 1246 5655 BstYI r'GATC_y Cuts at: 1972 3078 4231 4598 4983 5000 6452 6538 6550 Size: 1106 1153 367 385 17 1452 86 12 Cuts at: 6550 6896 1972 Size: 346 1977 Fragments arranged by size: 1977 1452 1153 1106 385 367 346 86 17 12 BstZ17I GTA'TAC Cuts at: 3015 3015 Size: 6901 Bsu36I CC'TnA_GG Cuts at: 3987 3987 Size: 6901 BtgI C'CryG_G Cuts at: 513 1065 2004 3507 3523 3624 3766 513 Size: 552 939 1503 16 101 142 3648 Fragments arranged by size: 3648 1503 939 552 142 101 16 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 2580 3835 487 Size: 683 56 1354 1255 3553 Fragments arranged by size: 3553 1354 1255 683 56 BtsI GCAGTG_nn' Cuts at: 3549 3708 3925 4417 5211 5231 3549 Size: 159 217 492 794 20 5219 Fragments arranged by size: 5219 794 492 217 159 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 6901 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 3613 3648 3726 3761 529 Size: 35 3049 35 78 35 3669 Fragments arranged by size: 3669 3049 78 35 35 35 DraI TTT'AAA Cuts at: 4205 4205 Size: 6901 DraIII CAC_nnn'GTG Cuts at: 2151 2151 Size: 6901 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 5908 809 Size: 507 4592 1802 Fragments arranged by size: 4592 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2125 3634 4348 Size: 54 1425 50 275 18 295 1509 714 Cuts at: 4348 5237 6866 8 Size: 889 1629 43 Fragments arranged by size: 1629 1509 1425 889 714 295 275 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 4348 1537 Size: 275 2536 4090 Fragments arranged by size: 4090 2536 275 EarI CTCTTCn'nnn_ Cuts at: 1353 2346 2917 4831 1353 Size: 993 571 1914 3423 Fragments arranged by size: 3423 1914 993 571 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 5488 5862 6008 387 Size: 1064 138 3899 374 146 1280 Fragments arranged by size: 3899 1280 1064 374 146 138 EcoICRI GAG'CTC Cuts at: 2884 2884 Size: 6901 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2283 2352 2387 2438 2606 2784 3234 Size: 393 651 69 35 51 168 178 450 Cuts at: 3234 3380 3471 3701 4964 5561 6348 6616 1239 Size: 146 91 230 1263 597 787 268 1524 Fragments arranged by size: 1524 1263 787 651 597 450 393 268 230 178 168 146 91 69 51 35 EcoNI CCTnn'n_nnAGG Cuts at: 3324 3362 3324 Size: 38 6863 EcoO109I rG'GnC_Cy Cuts at: 2059 2164 2243 2342 2722 2758 3163 3256 3530 Size: 105 79 99 380 36 405 93 274 Cuts at: 3530 3531 3819 2059 Size: 1 288 5141 Fragments arranged by size: 5141 405 380 288 274 105 99 93 79 36 1 EcoRI G'AATT_C Cuts at: 4210 4588 4210 Size: 378 6523 FalI AAGnnnnnCTTnnnnnnnn_nnnnn' Cuts at: 3607 3607 Size: 6901 FspI TGC'GCA Cuts at: 2324 5407 2324 Size: 3083 3818 HaeII r_GCGC'y Cuts at: 1678 2088 2417 2515 6048 1678 Size: 410 329 98 3533 2531 Fragments arranged by size: 3533 2531 410 329 98 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2210 2242 2720 2752 2962 Size: 32 135 32 638 32 478 32 210 Cuts at: 2962 2994 4133 4165 5542 5574 5616 5648 1373 Size: 32 1139 32 1377 32 42 32 2626 Fragments arranged by size: 2626 1377 1139 638 478 210 135 42 32 32 32 32 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 4245 4491 669 Size: 988 281 2307 246 3079 Fragments arranged by size: 3079 2307 988 281 246 HindIII A'AGCT_T Cuts at: 748 748 Size: 6901 HpaI GTT'AAC Cuts at: 4491 4491 Size: 6901 KpnI G_GTAC'C Cuts at: 4226 4226 Size: 6901 MfeI C'AATT_G Cuts at: 3246 4500 3246 Size: 1254 5647 MluI A'CGCG_T Cuts at: 6777 6777 Size: 6901 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 3320 6015 6199 1305 Size: 2015 2695 184 2007 Fragments arranged by size: 2695 2015 2007 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 3636 10 Size: 54 1425 343 1804 3275 Fragments arranged by size: 3275 1804 1425 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 2669 2708 3185 3401 3678 3732 4859 Size: 648 1503 39 477 216 277 54 1127 Cuts at: 4859 5218 5377 518 Size: 359 159 2042 Fragments arranged by size: 2042 1503 1127 648 477 359 277 216 159 54 39 MspA1I CmG'CkG Cuts at: 1606 2995 3058 3116 3212 4317 4994 6142 6387 Size: 1389 63 58 96 1105 677 1148 245 Cuts at: 6387 1606 Size: 2120 Fragments arranged by size: 2120 1389 1148 1105 677 245 96 63 58 NaeI GCC'GGC Cuts at: 3158 3158 Size: 6901 NcoI C'CATG_G Cuts at: 513 1065 2004 3507 3766 513 Size: 552 939 1503 259 3648 Fragments arranged by size: 3648 1503 939 552 259 NdeI CA'TA_TG Cuts at: 387 387 Size: 6901 NgoMIV G'CCGG_C Cuts at: 3156 3156 Size: 6901 NheI G'CTAG_C Cuts at: 1052 1052 Size: 6901 NotI GC'GGCC_GC Cuts at: 4348 4348 Size: 6901 NspI r_CATG'y Cuts at: 1132 1229 4259 5804 1132 Size: 97 3030 1545 2229 Fragments arranged by size: 3030 2229 1545 97 PasI CC'CwG_GG Cuts at: 1348 2345 1348 Size: 997 5904 PciI A'CATG_T Cuts at: 5800 5800 Size: 6901 PflMI CCAn_nnn'nTGG Cuts at: 1108 2206 3130 1108 Size: 1098 924 4879 Fragments arranged by size: 4879 1098 924 PfoI T'CCnGG_A Cuts at: 2364 3717 2364 Size: 1353 5548 PmeI GTTT'AAAC Cuts at: 4205 4205 Size: 6901 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 2345 2377 3699 3731 4970 5002 6483 6515 2345 Size: 32 1322 32 1239 32 1481 32 2731 Fragments arranged by size: 2731 1481 1322 1239 32 32 32 32 PpuMI rG'GwC_Cy Cuts at: 2164 2243 2722 3163 3256 2164 Size: 79 479 441 93 5809 Fragments arranged by size: 5809 479 441 93 79 PshAI GACnn'nnGTC Cuts at: 1613 2937 1613 Size: 1324 5577 PsiI TTA'TAA Cuts at: 4471 4471 Size: 6901 PspOMI G'GGCC_C Cuts at: 2098 2341 2757 3530 2098 Size: 243 416 773 5469 Fragments arranged by size: 5469 773 416 243 PstI C_TGCA'G Cuts at: 830 2251 2958 3089 3328 3474 4253 830 Size: 1421 707 131 239 146 779 3478 Fragments arranged by size: 3478 1421 779 707 239 146 131 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 6901 PvuII CAG'CTG Cuts at: 2995 3058 3116 3212 2995 Size: 63 58 96 6684 Fragments arranged by size: 6684 96 63 58 SacI G_AGCT'C Cuts at: 2886 2886 Size: 6901 SalI G'TCGA_C Cuts at: 1936 4243 1936 Size: 2307 4594 SanDI GG'GwC_CC Cuts at: 2722 2722 Size: 6901 SbfI CC_TGCA'GG Cuts at: 2251 4253 2251 Size: 2002 4899 ScaI AGT'ACT Cuts at: 5149 6861 5149 Size: 1712 5189 SexAI A'CCwGG_T Cuts at: 2205 2205 Size: 6901 SfcI C'TryA_G Cuts at: 826 1045 2247 2954 3085 3324 3407 3470 3814 Size: 219 1202 707 131 239 83 63 344 Cuts at: 3814 4249 5384 6065 6256 826 Size: 435 1135 681 191 1471 Fragments arranged by size: 1471 1202 1135 707 681 435 344 239 219 191 131 83 63 SfiI GGCCn_nnn'nGGCC Cuts at: 3827 3827 Size: 6901 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 6901 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 6901 SmaI CCC'GGG Cuts at: 1872 2270 4228 1872 Size: 398 1958 4545 Fragments arranged by size: 4545 1958 398 SmlI C'TyrA_G Cuts at: 820 1017 1943 2393 2714 4073 5005 5906 6168 Size: 197 926 450 321 1359 932 901 262 Cuts at: 6168 820 Size: 1553 Fragments arranged by size: 1553 1359 932 926 901 450 321 262 197 SnaBI TAC'GTA Cuts at: 493 4692 493 Size: 4199 2702 SpeI A'CTAG_T Cuts at: 152 3604 152 Size: 3452 3449 SphI G_CATG'C Cuts at: 1132 4259 1132 Size: 3127 3774 SspI AAT'ATT Cuts at: 5 52 4825 5 Size: 47 4773 2081 Fragments arranged by size: 4773 2081 47 StuI AGG'CCT Cuts at: 1517 1517 Size: 6901 StyI C'CwwG_G Cuts at: 513 1065 2004 2517 2547 3018 3507 3766 4340 Size: 552 939 513 30 471 489 259 574 Cuts at: 4340 513 Size: 3074 Fragments arranged by size: 3074 939 574 552 513 489 471 259 30 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 3197 3298 3340 3746 4136 4928 4945 Size: 1255 1617 101 42 406 390 792 17 Cuts at: 4945 5098 5283 5728 325 Size: 153 185 445 1498 Fragments arranged by size: 1617 1498 1255 792 445 406 390 185 153 101 42 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 5147 6859 96 Size: 274 80 33 51 1443 3170 1712 138 Fragments arranged by size: 3170 1712 1443 274 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 6861 6893 6861 Size: 32 6869 Tth111I GACn'n_nGTC Cuts at: 1316 1316 Size: 6901 XbaI T'CTAG_A Cuts at: 4237 4237 Size: 6901 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 6901 XmaI C'CCGG_G Cuts at: 1870 2268 4226 1870 Size: 398 1958 4545 Fragments arranged by size: 4545 1958 398 XmnI GAAnn'nnTTC Cuts at: 2360 5030 2360 Size: 2670 4231 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 2554 4709 276 Size: 53 83 186 936 1020 2155 2468 Fragments arranged by size: 2468 2155 1020 936 186 83 53 Enzymes that do cut: AatII AccI Acc65I AclI AcuI AfeI AflII AflIII AgeI AhdI AleI AlfI AloI AlwNI ApaI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbsI BbvCI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BplI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI BstZ17I Bsu36I BtgI BtgZI BtsI ClaI CspCI DraI DraIII DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoNI EcoO109I EcoRI FalI FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I NaeI NcoI NdeI NgoMIV NheI NotI NspI PasI PciI PflMI PfoI PmeI PpiI PpuMI PshAI PsiI PspOMI PstI PvuI PvuII SacI SalI SanDI SbfI ScaI SexAI SfcI SfiI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XhoI XmaI XmnI ZraI Enzymes that do not cut: AarI AscI AvrII BaeI BbeI BfrBI BsiWI BsmBI EcoRV FseI FspAI KasI NarI NruI NsiI PacI PmlI PspXI PsrI RsrII SacII SapI SfoI SrfI SwaI XcmI