(Linear) (Six-base) MAPSORT of: KIEE5712.seq Check: 605 from: 1 to: 2196 KIEE5712.orf With 182 enzymes: SgfI * December 5, 2014 13:07 .. AatII G_ACGT'C Cuts at: 0 551 2196 Size: 551 1645 AccI GT'mk_AC Cuts at: 0 944 1009 2196 Size: 944 65 1187 Fragments arranged by size: 1187 944 65 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 0 278 601 2196 Size: 278 323 1595 Fragments arranged by size: 1595 323 278 AfeI AGC'GCT Cuts at: 0 410 2196 Size: 410 1786 AleI CACnn'nnGTG Cuts at: 0 1180 2196 Size: 1180 1016 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 0 682 1105 1414 2196 Size: 682 423 309 782 Fragments arranged by size: 782 682 423 309 AloI GAACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 0 1694 1726 2196 Size: 1694 32 470 Fragments arranged by size: 1694 470 32 AlwNI CAG_nnn'CTG Cuts at: 0 15 241 360 1161 1817 1919 2196 Size: 15 226 119 801 656 102 277 Fragments arranged by size: 801 656 277 226 119 102 15 ApaI G_GGCC'C Cuts at: 0 97 340 756 1529 2196 Size: 97 243 416 773 667 Fragments arranged by size: 773 667 416 243 97 ApaLI G'TGCA_C Cuts at: 0 136 2196 Size: 136 2060 AvaI C'yCGr_G Cuts at: 0 28 263 2196 Size: 28 235 1933 Fragments arranged by size: 1933 235 28 BanI G'GyrC_C Cuts at: 0 298 2196 Size: 298 1898 BanII G_rGCy'C Cuts at: 0 29 97 340 756 800 881 925 1370 Size: 29 68 243 416 44 81 44 445 Cuts at: 1370 1529 1864 2196 Size: 159 335 332 Fragments arranged by size: 445 416 335 332 243 159 81 68 44 44 29 BbsI GAAGACnn'nnnn_ Cuts at: 0 635 1996 2196 Size: 635 1361 200 Fragments arranged by size: 1361 635 200 BbvCI CC'TCA_GC Cuts at: 0 1933 2196 Size: 1933 263 BclI T'GATC_A Cuts at: 0 1838 2196 Size: 1838 358 BglI GCCn_nnn'nGGC Cuts at: 0 1822 2091 2196 Size: 1822 269 105 Fragments arranged by size: 1822 269 105 BlpI GC'TnA_GC Cuts at: 0 19 76 277 919 1142 2196 Size: 19 57 201 642 223 1054 Fragments arranged by size: 1054 642 223 201 57 19 Bme1580I G_kGCm'C Cuts at: 0 97 140 340 756 1189 1502 1529 2036 Size: 97 43 200 416 433 313 27 507 Cuts at: 2036 2067 2196 Size: 31 129 Fragments arranged by size: 507 433 416 313 200 129 97 43 31 27 BmrI ACTGGGnnnn_n' Cuts at: 0 642 1395 2196 Size: 642 753 801 Fragments arranged by size: 801 753 642 BplI GAGnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 0 900 1083 2196 Size: 900 183 1113 Fragments arranged by size: 1113 900 183 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 0 347 382 433 779 1229 1375 1466 1696 Size: 347 35 51 346 450 146 91 230 Cuts at: 1696 2196 Size: 500 Fragments arranged by size: 500 450 347 346 230 146 91 51 35 Bpu10I CC'TnA_GC Cuts at: 0 58 1933 2196 Size: 58 1875 263 Fragments arranged by size: 1875 263 58 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 0 373 730 2053 2196 Size: 373 357 1323 143 Fragments arranged by size: 1323 373 357 143 BsaI GGTCTCn'nnnn_ Cuts at: 0 253 1798 2196 Size: 253 1545 398 Fragments arranged by size: 1545 398 253 BsaAI yAC'GTr Cuts at: 0 852 2196 Size: 852 1344 BsaBI GATnn'nnATC Cuts at: 0 1843 2196 Size: 1843 353 BsaHI Gr'CG_yC Cuts at: 0 548 2196 Size: 548 1648 BsaWI w'CCGG_w Cuts at: 0 128 380 803 2196 Size: 128 252 423 1393 Fragments arranged by size: 1393 423 252 128 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 0 1694 1724 1834 1864 2128 2158 2196 Size: 1694 30 110 30 264 30 38 Fragments arranged by size: 1694 264 110 38 30 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 0 46 871 1372 2196 Size: 46 825 501 824 Fragments arranged by size: 825 824 501 46 BseYI C'CCAG_C Cuts at: 0 1053 1279 1563 2196 Size: 1053 226 284 633 Fragments arranged by size: 1053 633 284 226 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 0 1450 2196 Size: 1450 746 BsiHKAI G_wGCw'C Cuts at: 0 140 671 881 1712 2171 2196 Size: 140 531 210 831 459 25 Fragments arranged by size: 831 531 459 210 140 25 BsmI GAATG_Cn' Cuts at: 0 2166 2196 Size: 2166 30 Bsp1286I G_dGCh'C Cuts at: 0 29 97 140 340 671 756 800 881 Size: 29 68 43 200 331 85 44 81 Cuts at: 881 925 1189 1370 1502 1529 1712 1864 2036 Size: 44 264 181 132 27 183 152 172 Cuts at: 2036 2067 2171 2196 Size: 31 104 25 Fragments arranged by size: 331 264 200 183 181 172 152 132 104 85 81 68 44 44 43 31 29 27 25 BspHI T'CATG_A Cuts at: 0 2156 2196 Size: 2156 40 BspMI ACCTGCnnnn'nnnn_ Cuts at: 0 249 824 956 2196 Size: 249 575 132 1240 Fragments arranged by size: 1240 575 249 132 BsrDI GCAATG_nn' Cuts at: 0 1665 2196 Size: 1665 531 BsrFI r'CCGG_y Cuts at: 0 1151 2024 2196 Size: 1151 873 172 Fragments arranged by size: 1151 873 172 BstAPI GCAn_nnn'nTGC Cuts at: 0 241 1919 2196 Size: 241 1678 277 Fragments arranged by size: 1678 277 241 BstEII G'GTnAC_C Cuts at: 0 102 1017 2196 Size: 102 915 1179 Fragments arranged by size: 1179 915 102 BstXI CCAn_nnnn'nTGG Cuts at: 0 543 1789 2196 Size: 543 1246 407 Fragments arranged by size: 1246 543 407 BstYI r'GATC_y Cuts at: 0 1073 2196 Size: 1073 1123 BstZ17I GTA'TAC Cuts at: 0 1010 2196 Size: 1010 1186 Bsu36I CC'TnA_GG Cuts at: 0 1982 2196 Size: 1982 214 BtgI C'CryG_G Cuts at: 0 1502 1518 1619 1761 2196 Size: 1502 16 101 142 435 Fragments arranged by size: 1502 435 142 101 16 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 0 575 1830 2196 Size: 575 1255 366 Fragments arranged by size: 1255 575 366 BtsI GCAGTG_nn' Cuts at: 0 1544 1703 1920 2196 Size: 1544 159 217 276 Fragments arranged by size: 1544 276 217 159 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 0 1608 1643 1721 1756 2196 Size: 1608 35 78 35 440 Fragments arranged by size: 1608 440 78 35 35 DraIII CAC_nnn'GTG Cuts at: 0 146 2196 Size: 146 2050 EaeI y'GGCC_r Cuts at: 0 120 1629 2196 Size: 120 1509 567 Fragments arranged by size: 1509 567 120 EarI CTCTTCn'nnn_ Cuts at: 0 341 912 2196 Size: 341 571 1284 Fragments arranged by size: 1284 571 341 EcoICRI GAG'CTC Cuts at: 0 879 2196 Size: 879 1317 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 0 278 347 382 433 601 779 1229 1375 Size: 278 69 35 51 168 178 450 146 Cuts at: 1375 1466 1696 2196 Size: 91 230 500 Fragments arranged by size: 500 450 278 230 178 168 146 91 69 51 35 EcoNI CCTnn'n_nnAGG Cuts at: 0 1319 1357 2196 Size: 1319 38 839 Fragments arranged by size: 1319 839 38 EcoO109I rG'GnC_Cy Cuts at: 0 54 159 238 337 717 753 1158 1251 Size: 54 105 79 99 380 36 405 93 Cuts at: 1251 1525 1526 1814 2196 Size: 274 1 288 382 Fragments arranged by size: 405 382 380 288 274 105 99 93 79 54 36 1 FalI AAGnnnnnCTTnnnnnnnn_nnnnn' Cuts at: 0 1602 2196 Size: 1602 594 FspI TGC'GCA Cuts at: 0 319 2196 Size: 319 1877 HaeII r_GCGC'y Cuts at: 0 83 412 510 2196 Size: 83 329 98 1686 Fragments arranged by size: 1686 329 98 83 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 0 205 237 715 747 957 989 2128 2160 Size: 205 32 478 32 210 32 1139 32 Cuts at: 2160 2196 Size: 36 Fragments arranged by size: 1139 478 210 205 36 32 32 32 32 MfeI C'AATT_G Cuts at: 0 1241 2196 Size: 1241 955 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 0 1315 2196 Size: 1315 881 MscI TGG'CCA Cuts at: 0 1631 2196 Size: 1631 565 MslI CAynn'nnrTG Cuts at: 0 664 703 1180 1396 1673 1727 2196 Size: 664 39 477 216 277 54 469 Fragments arranged by size: 664 477 469 277 216 54 39 MspA1I CmG'CkG Cuts at: 0 990 1053 1111 1207 2196 Size: 990 63 58 96 989 Fragments arranged by size: 990 989 96 63 58 NaeI GCC'GGC Cuts at: 0 1153 2196 Size: 1153 1043 NcoI C'CATG_G Cuts at: 0 1502 1761 2196 Size: 1502 259 435 Fragments arranged by size: 1502 435 259 NgoMIV G'CCGG_C Cuts at: 0 1151 2196 Size: 1151 1045 PasI CC'CwG_GG Cuts at: 0 340 2196 Size: 340 1856 PflMI CCAn_nnn'nTGG Cuts at: 0 201 1125 2196 Size: 201 924 1071 Fragments arranged by size: 1071 924 201 PfoI T'CCnGG_A Cuts at: 0 359 1712 2196 Size: 359 1353 484 Fragments arranged by size: 1353 484 359 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 0 340 372 1694 1726 2196 Size: 340 32 1322 32 470 Fragments arranged by size: 1322 470 340 32 32 PpuMI rG'GwC_Cy Cuts at: 0 159 238 717 1158 1251 2196 Size: 159 79 479 441 93 945 Fragments arranged by size: 945 479 441 159 93 79 PshAI GACnn'nnGTC Cuts at: 0 932 2196 Size: 932 1264 PspOMI G'GGCC_C Cuts at: 0 93 336 752 1525 2196 Size: 93 243 416 773 671 Fragments arranged by size: 773 671 416 243 93 PstI C_TGCA'G Cuts at: 0 246 953 1084 1323 1469 2196 Size: 246 707 131 239 146 727 Fragments arranged by size: 727 707 246 239 146 131 PvuII CAG'CTG Cuts at: 0 990 1053 1111 1207 2196 Size: 990 63 58 96 989 Fragments arranged by size: 990 989 96 63 58 SacI G_AGCT'C Cuts at: 0 881 2196 Size: 881 1315 SanDI GG'GwC_CC Cuts at: 0 717 2196 Size: 717 1479 SbfI CC_TGCA'GG Cuts at: 0 246 2196 Size: 246 1950 SexAI A'CCwGG_T Cuts at: 0 200 2196 Size: 200 1996 SfcI C'TryA_G Cuts at: 0 242 949 1080 1319 1402 1465 1809 2196 Size: 242 707 131 239 83 63 344 387 Fragments arranged by size: 707 387 344 242 239 131 83 63 SfiI GGCCn_nnn'nGGCC Cuts at: 0 1822 2196 Size: 1822 374 SmaI CCC'GGG Cuts at: 0 265 2196 Size: 265 1931 SmlI C'TyrA_G Cuts at: 0 388 709 2068 2196 Size: 388 321 1359 128 Fragments arranged by size: 1359 388 321 128 SpeI A'CTAG_T Cuts at: 0 1599 2196 Size: 1599 597 StyI C'CwwG_G Cuts at: 0 512 542 1013 1502 1761 2196 Size: 512 30 471 489 259 435 Fragments arranged by size: 512 489 471 435 259 30 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 0 1192 1293 1335 1741 2131 2196 Size: 1192 101 42 406 390 65 Fragments arranged by size: 1192 406 390 101 65 42 XmaI C'CCGG_G Cuts at: 0 263 2196 Size: 263 1933 XmnI GAAnn'nnTTC Cuts at: 0 355 2196 Size: 355 1841 ZraI GAC'GTC Cuts at: 0 549 2196 Size: 549 1647 Enzymes that do cut: AatII AccI AcuI AfeI AleI AlfI AloI AlwNI ApaI ApaLI AvaI BanI BanII BbsI BbvCI BclI BglI BlpI Bme1580I BmrI BplI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiHKAI BsmI Bsp1286I BspHI BspMI BsrDI BsrFI BstAPI BstEII BstXI BstYI BstZ17I Bsu36I BtgI BtgZI BtsI CspCI DraIII EaeI EarI EcoICRI Eco57MI EcoNI EcoO109I FalI FspI HaeII Hin4I MfeI MmeI MscI MslI MspA1I NaeI NcoI NgoMIV PasI PflMI PfoI PpiI PpuMI PshAI PspOMI PstI PvuII SacI SanDI SbfI SexAI SfcI SfiI SmaI SmlI SpeI StyI TaqII XmaI XmnI ZraI Enzymes that do not cut: AarI Acc65I AclI AflII AflIII AgeI AhdI ApoI AscI AseI AsiSI AvrII BaeI BamHI BbeI BcgI BciVI BfrBI BglII BmgBI BmtI BsiEI BsiWI BsmBI BspEI BsrBI BsrGI BssHII BssSI BstBI ClaI DraI DrdI EagI EciI EcoRI EcoRV FseI FspAI HincII HindIII HpaI KasI KpnI MluI NarI NdeI NheI NotI NruI NsiI NspI PacI PciI PmeI PmlI PsiI PspXI PsrI PvuI RsrII SacII SalI SapI ScaI SfoI SgfI SgrAI SnaBI SphI SrfI SspI StuI SwaI TatI TstI Tth111I XbaI XcmI XhoI