################################## # NO CUT ENZYMES ( 149 enzymes) # ################################## AatII Acc65I AccIII AclI AcsI AflII AflIII AgeI AhdI Alw44I AlwI AocI Aor51HI ApaI ApaLI ApoI AscI AseI AsnI Asp700I Asp718I AspEI AspI AviII AvrII BalI BamHI BbrPI BbvCI BcgI BciVI BclI BfrI BglI BglII BlnI BlpI Bpu1102I BsaAI BsaBI BsaI BsaWI BseAI BseRI BsiWI BsmBI BsmI Bsp1407I BspDI BspEI BspHI BspLU11I BsrDI BsrFI BsrGI BsrI BssHII BssSI Bst1107I BstAPI BstBI BstEII BstF5I BstPI BstYI BstZ17I Bsu36I CelII Cfr10I ClaI CpoI DraI DrdI DsaI Eam1105I Eco47III Eco81I EcoNI EcoO65I EcoRI EcoRV EcoT14I EcoT22I FbaI FokI FseI FspI HindIII HpaI HphI KpnI KspI MamI MfeI MflI MluI MluNI MroI MscI MslI MspA1I MunI NaeI NcoI NgoMIV NheI NotI NruI NsiI NspI NspV PacI PaeR7I PflFI PinAI PmaCI PmeI PmlI PshAI PshBI Psp1406I PstI PvuI PvuII RcaI RsrII SacII SapI SbfI ScaI SfcI SfiI SfuI SgrAI SnaBI SpeI SphI Sse8387I SspBI SspI StuI StyI SwaI Tsp509I Tth111I XbaI XhoI XhoII XmnI ################################### # ONE CUT ENZYMES ( 52 enzymes) # ################################### AccI 235 GTCGAC GT^[AC][GT]AC AcyI 212 GGCGCC G[GA]^CG[CT]C AfaI 529 GTAC GT^AC AlwNI 495 CAGGTCCTG CAG[ACGT][ACGT][ACGT]^CTG BanI 212 GGCGCC G^G[CT][GA]CC BbeI 212 GGCGCC GGCGC^C BbsI 283 GAAGAC GAAGAC(2/6) BfaI 127 CTAG C^TAG BpuAI 283 GAAGAC GAAGAC(2/6) BsaHI 212 GGCGCC G[GA]^CG[CT]C BsiEI 260 CGGCCG CG[GA][CT]^CG BsmAI 361 GTCTC GTCTC(1/5) BsmFI 579 GTCCC GGGAC(10/14) BspMI 221 ACCTGC ACCTGC(4/8) BsrBI 120 GAGCGG CCGCTC(-3/-3) BstXI 140 CCATGCTGCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG DpnII 375 GATC ^GATC DraII 496 AGGTCCT [GA]G^G[ACGT]CC[CT] EaeI 260 CGGCCG [CT]^GGCC[GA] EagI 260 CGGCCG C^GGCCG EarI 133 CTCTTC CTCTTC(1/4) EclXI 260 CGGCCG C^GGCCG Eco52I 260 CGGCCG C^GGCCG EcoO109I 496 AGGTCCT [GA]G^G[ACGT]CC[CT] HaeII 212 GGCGCC [GA]GCGC^[CT] Hin1I 212 GGCGCC G[GA]^CG[CT]C HincII 235 GTCGAC GT[CT]^[GA]AC HindII 235 GTCGAC GT[CT]^[GA]AC KasI 212 GGCGCC G^GCGCC Ksp632I 133 CTCTTC CTCTTC(1/4) MaeI 127 CTAG C^TAG MaeII 305 ACGT A^CGT MboI 375 GATC ^GATC MseI 302 TTAA T^TAA NarI 212 GGCGCC GG^CGCC NdeI 309 CATATG CA^TATG NdeII 375 GATC ^GATC PflMI 477 CCACCTGGTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG PleI 427 GAGTC GAGTC(4/5) PpuMI 496 AGGTCCT [GA]G^G[AT]CC[CT] RsaI 529 GTAC GT^AC SalI 235 GTCGAC G^TCGAC Sau3AI 375 GATC ^GATC SfoI 212 GGCGCC GGC^GCC SmaI 169 CCCGGG CCC^GGG SmlI 67 CTCAAG C^T[CT][GA]AG TfiI 437 GAATC G^A[AT]TC Tru9I 302 TTAA T^TAA Van91I 477 CCACCTGGTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG XcmI 558 CCAGAACAGGAGTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG XmaCI 169 CCCGGG C^CCGGG XmaI 169 CCCGGG C^CCGGG ################################### # TWO CUT ENZYMES ( 15 enzymes) # ################################### AspHI 64 GAGCTC G[AT]GC[AT]^C 181 GAGCTC AvaI 82 CTCGGG C^[CT]CG[GA]G 169 CCCGGG AvaII 3 GGACC G^G[AT]CC 497 GGTCC BsiHKAI 64 GAGCTC G[AT]GC[AT]^C 181 GAGCTC BsoBI 82 CTCGGG C^[CT]CG[GA]G 169 CCCGGG DraIII 25 CACTCGGTG CAC[ACGT][ACGT][ACGT]^GTG 478 CACCTGGTG HapII 170 CCGG C^CGG 263 CCGG HinfI 427 GAGTC G^A[ACGT]TC 437 GAATC HpaII 170 CCGG C^CGG 263 CCGG MaeIII 429 GTCAC ^GT[ACGT]AC 551 GTGAC MspI 170 CCGG C^CGG 263 CCGG SacI 64 GAGCTC GAGCT^C 181 GAGCTC SfaNI 383 GCATC GCATC(5/9) 604 GCATC Tsp45I 429 GTCAC ^GT[GC]AC 551 GTGAC TspRI 431 CACTG CA[GC]TG[ACGT][ACGT]^ 470 CAGTG ######################################### # 3 OR MORE CUT ENZYMES ( 42 enzymes) # ######################################### AccII AciI AluI BanII BbvI BcnI BmyI BpmI BsaJI BsgI BsiYI BslI Bsp1286I BssKI BstNI BstUI Cac8I CfoI Cfr13I DdeI EcoRII FauI Fnu4HI HaeIII HgaI HhaI HinP1I ItaI MboII MnlI MvaI MvnI MwoI NciI NlaIII NlaIV Sau96I ScrFI SexAI TaqI TseI TthHB8I