################################## # NO CUT ENZYMES ( 189 enzymes) # ################################## AatII Acc65I AccI AccII AccIII AciI AclI AcyI AflII AflIII AgeI AhdI Alw44I AocI Aor51HI ApaI ApaLI AscI Asp718I AspEI AspHI AspI AvaI AviII AvrII BalI BamHI BanI BanII BbeI BbrPI BbvCI BcgI BclI BcnI BfrI BglI BlnI BlpI BmyI BpmI Bpu1102I BsaAI BsaBI BsaHI BsaI BsaWI BseAI BseRI BsiEI BsiHKAI BsiWI BsiYI BslI BsmBI BsoBI Bsp1286I Bsp1407I BspDI BspEI BspHI BspLU11I BspMI BsrBI BsrFI BsrGI BssHII BssKI BssSI Bst1107I BstAPI BstBI BstEII BstF5I BstNI BstPI BstUI BstXI BstZ17I Bsu36I Cac8I CelII CfoI Cfr10I ClaI CpoI DraI DraII DraIII DrdI DsaI EaeI EagI Eam1105I EclXI Eco47III Eco52I Eco81I EcoNI EcoO109I EcoO65I EcoRI EcoRII EcoRV EcoT22I FauI FbaI FokI FseI FspI HaeII HapII HgaI HhaI Hin1I HinP1I HindIII HpaII KasI KpnI KspI MaeII MaeIII MamI MluI MluNI MroI MscI MspI MvaI MvnI NaeI NarI NciI NcoI NdeI NgoMIV NheI NotI NruI NsiI NspV PacI PaeR7I PflFI PflMI PinAI PmaCI PmeI PmlI PpuMI PshAI Psp1406I PstI PvuI RcaI RsrII SacI SacII SalI SapI SbfI ScaI ScrFI SexAI SfaNI SfiI SfoI SfuI SgrAI SmaI SmlI SnaBI SpeI SphI Sse8387I SspBI StuI SwaI TaqI Tsp45I TspRI Tth111I TthHB8I Van91I XcmI XhoI XmaCI XmaI ################################### # ONE CUT ENZYMES ( 41 enzymes) # ################################### AcsI 231 GAATTT [GA]^AATT[CT] AlwNI 31 CAGAAACTG CAG[ACGT][ACGT][ACGT]^CTG ApoI 231 GAATTT [GA]^AATT[CT] AseI 529 ATTAAT AT^TAAT AsnI 529 ATTAAT AT^TAAT Asp700I 673 GAATGTCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC AvaII 879 GGTCC G^G[AT]CC BbsI 677 GTCTTC GAAGAC(2/6) BbvI 299 GCTGC GCAGC(8/12) BciVI 733 GTATCC GTATCC(6/5) BpuAI 677 GTCTTC GAAGAC(2/6) BsaJI 279 CCTTGG C^C[ACGT][ACGT]GG BsmI 51 GAATGC GAATGC(1/-1) BsrI 237 ACTGG ACTGG(1/-1) Cfr13I 879 GGTCC G^G[ACGT]CC EarI 328 GAAGAG CTCTTC(1/4) EcoT14I 279 CCTTGG C^C[AT][AT]GG Fnu4HI 299 GCTGC GC^[ACGT]GC HaeIII 130 GGCC GG^CC HincII 657 GTTAAC GT[CT]^[GA]AC HindII 657 GTTAAC GT[CT]^[GA]AC HpaI 657 GTTAAC GTT^AAC HphI 855 TCACC GGTGA(8/7) ItaI 299 GCTGC GC^[ACGT]GC Ksp632I 328 GAAGAG CTCTTC(1/4) MfeI 826 CAATTG C^AATTG MspA1I 46 CAGCTG C[AC]G^C[GT]G MunI 826 CAATTG C^AATTG NlaIII 563 CATG CATG^ NlaIV 879 GGTCCC GG[ACGT]^[ACGT]CC NspI 562 GCATGT [GA]CATG^[CT] PshBI 529 ATTAAT AT^TAAT PvuII 46 CAGCTG CAG^CTG Sau96I 879 GGTCC G^G[ACGT]CC SfcI 843 CTACAG C^T[GA][CT]AG SspI 752 AATATT AAT^ATT StyI 279 CCTTGG C^C[AT][AT]GG TfiI 245 GATTC G^A[AT]TC TseI 299 GCTGC G^C[AT]GC XbaI 73 TCTAGA T^CTAGA XmnI 673 GAATGTCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC ################################### # TWO CUT ENZYMES ( 5 enzymes) # ################################### BglII 171 AGATCT A^GATCT 708 AGATCT BsgI 116 CTGCAC GTGCAG(16/14) 818 CTGCAC BsmAI 102 GAGAC GTCTC(1/5) 211 GAGAC BsrDI 568 CATTGC GCAATG(2/0) 621 CATTGC MslI 121 CACGATAATG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG 641 CACAGAAGTG ######################################### # 3 OR MORE CUT ENZYMES ( 23 enzymes) # ######################################### AfaI AluI AlwI BfaI BsmFI BstYI DdeI DpnII HinfI MaeI MboI MboII MflI MnlI MseI MwoI NdeII PleI RsaI Sau3AI Tru9I Tsp509I XhoII