################################## # NO CUT ENZYMES ( 137 enzymes) # ################################## AatII Acc65I AccI AccII AccIII AclI AflII AgeI Alw44I Aor51HI ApaI ApaLI AscI AseI AsnI Asp700I Asp718I AspI AviII AvrII BamHI BbeI BbrPI BbvCI BcgI BciVI BclI BfaI BfrI BglI BglII BlnI BlpI Bpu1102I BsaAI BsaBI BsaI BsaWI BseAI BsgI BsiEI BsiWI BsmBI BsmI Bsp1407I BspEI BspHI BsrBI BsrDI BsrGI BsrI BssHII Bst1107I BstBI BstEII BstPI BstUI BstXI BstZ17I CelII CpoI DraI DraIII DrdI EagI EarI EclXI Eco47III Eco52I EcoO65I EcoRI EcoT22I FauI FbaI FseI FspI HincII HindII HindIII HpaI KasI KpnI Ksp632I KspI MaeI MamI MfeI MluI MroI MunI MvnI NarI NdeI NheI NotI NruI NsiI NspV PacI PflFI PflMI PinAI PmaCI PmeI PmlI PshAI PshBI Psp1406I PstI PvuI RcaI RsrII SacI SacII SalI SapI SbfI SexAI SfiI SfoI SfuI SgrAI SmaI SnaBI SpeI SphI Sse8387I SspBI SspI StuI SwaI Tth111I Van91I XbaI XmaCI XmaI XmnI ################################### # ONE CUT ENZYMES ( 46 enzymes) # ################################### AcsI 580 AAATTT [GA]^AATT[CT] AflIII 842 ACATGT A^C[GA][CT]GT AhdI 205 GACCTGAAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC ApoI 580 AAATTT [GA]^AATT[CT] AspEI 205 GACCTGAAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC AspHI 177 GAGCAC G[AT]GC[AT]^C AvaI 827 CTCGAG C^[CT]CG[GA]G BalI 2 TGGCCA TGG^CCA BanII 91 GGGCTC G[GA]GC[CT]^C BbsI 106 GAAGAC GAAGAC(2/6) BpuAI 106 GAAGAC GAAGAC(2/6) BsiHKAI 177 GAGCAC G[AT]GC[AT]^C BsoBI 827 CTCGAG C^[CT]CG[GA]G BspDI 774 ATCGAT AT^CGAT BspLU11I 842 ACATGT A^CATGT BspMI 237 GCAGGT ACCTGC(4/8) BsrFI 310 GCCGGC [GA]^CCGG[CT] BssSI 514 CTCGTG CACGAG(-5/-1) Cfr10I 310 GCCGGC [GA]^CCGG[CT] ClaI 774 ATCGAT AT^CGAT DraII 533 AGGACCT [GA]G^G[ACGT]CC[CT] EaeI 2 TGGCCA [CT]^GGCC[GA] Eam1105I 205 GACCTGAAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC EcoO109I 533 AGGACCT [GA]G^G[ACGT]CC[CT] HaeII 149 AGCGCC [GA]GCGC^[CT] MaeII 278 ACGT A^CGT MaeIII 908 GTGAC ^GT[ACGT]AC MluNI 2 TGGCCA TGG^CCA MscI 2 TGGCCA TGG^CCA MseI 677 TTAA T^TAA MslI 810 CATCAGAGTG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG MspA1I 473 CAGCTG C[AC]G^C[GT]G NaeI 310 GCCGGC GCC^GGC NgoMIV 310 GCCGGC G^CCGGC PaeR7I 827 CTCGAG C^TCGAG PleI 872 GACTC GAGTC(4/5) PpuMI 533 AGGACCT [GA]G^G[AT]CC[CT] PvuII 473 CAGCTG CAG^CTG ScaI 933 AGTACT AGT^ACT SmlI 827 CTCGAG C^T[CT][GA]AG TfiI 44 GATTC G^A[AT]TC Tru9I 677 TTAA T^TAA Tsp45I 908 GTGAC ^GT[GC]AC TspRI 315 CACTG CA[GC]TG[ACGT][ACGT]^ XcmI 390 CCAGCAGCAATATGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG XhoI 827 CTCGAG C^TCGAG ################################### # TWO CUT ENZYMES ( 30 enzymes) # ################################### AciI 135 CCGC CCGC(-3/-1) 143 CCGC AcyI 109 GACGCC G[GA]^CG[CT]C 258 GACGCC AlwI 339 GATCC GGATC(4/5) 366 GATCC AlwNI 67 CAGACCCTG CAG[ACGT][ACGT][ACGT]^CTG 532 CAGGACCTG AocI 72 CCTGAGG CC^T[ACGT]AGG 358 CCTGAGG AvaII 353 GGACC G^G[AT]CC 534 GGACC BanI 97 GGCACC G^G[CT][GA]CC 790 GGCACC BpmI 548 CTGGAG CTGGAG(16/14) 917 CTGGAG BsaHI 109 GACGCC G[GA]^CG[CT]C 258 GACGCC BseRI 361 GAGGAG GAGGAG(10/8) 953 GAGGAG BsmFI 191 GGGAC GGGAC(10/14) 565 GGGAC BstAPI 282 GCAAGAGCTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC 748 GCATATTTTGC BstYI 338 AGATCC [GA]^GATC[CT] 365 AGATCC Bsu36I 72 CCTGAGG CC^T[ACGT]AGG 358 CCTGAGG Cac8I 310 GCCGGC GC[ACGT]^[ACGT]GC 714 GCTGGC CfoI 150 GCGC GCG^C 372 GCGC Eco81I 72 CCTGAGG CC^T[ACGT]AGG 358 CCTGAGG EcoNI 72 CCTGAGGAAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG 537 CCTGTATGAGG EcoRV 648 GATATC GAT^ATC 850 GATATC EcoT14I 5 CCATGG C^C[AT][AT]GG 53 CCATGG HhaI 150 GCGC GCG^C 372 GCGC Hin1I 109 GACGCC G[GA]^CG[CT]C 258 GACGCC HinP1I 150 GCGC G^CGC 372 GCGC HinfI 44 GATTC G^A[ACGT]TC 872 GACTC MflI 338 AGATCC [GA]^GATC[CT] 365 AGATCC NcoI 5 CCATGG C^CATGG 53 CCATGG NspI 624 GCATGT [GA]CATG^[CT] 842 ACATGT SfcI 719 CTATAG C^T[GA][CT]AG 924 CTACAG StyI 5 CCATGG C^C[AT][AT]GG 53 CCATGG XhoII 338 AGATCC [GA]^GATC[CT] 365 AGATCC ######################################### # 3 OR MORE CUT ENZYMES ( 45 enzymes) # ######################################### AfaI AluI BbvI BcnI BmyI BsaJI BsiYI BslI BsmAI Bsp1286I BssKI BstF5I BstNI Cfr13I DdeI DpnII DsaI EcoRII Fnu4HI FokI HaeIII HapII HgaI HpaII HphI ItaI MboI MboII MnlI MspI MvaI MwoI NciI NdeII NlaIII NlaIV RsaI Sau3AI Sau96I ScrFI SfaNI TaqI TseI Tsp509I TthHB8I