Restriction map for
pFN21AB6602
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# NO CUT ENZYMES ( 112 enzymes) #
##################################
AatII Acc65I AccI AccIII AclI
AcsI AflII AflIII Alw44I Aor51HI
ApaLI ApoI AscI AseI AsnI
Asp700I Asp718I AspI AviII AvrII
BbrPI BbsI BcgI BciVI BclI
BfrI BglI BlnI BlpI Bpu1102I
BpuAI BsaAI BsaBI BseAI BsiWI
BsmI Bsp1407I BspDI BspEI BspLU11I
BspMI BsrDI BsrGI BssHII BssSI
Bst1107I BstAPI BstBI BstZ17I CelII
ClaI CpoI DraI DraIII Eco47III
EcoRI EcoRV EcoT22I FbaI FseI
FspI HindIII HpaI KpnI KspI
MamI MfeI MluI MroI MunI
NaeI NdeI NgoMIV NheI NotI
NruI NsiI NspI NspV PacI
PaeR7I PflFI PmaCI PmeI PmlI
PshAI PshBI Psp1406I PvuI RsrII
SacI SacII SalI SbfI SexAI
SfiI SfuI SgrAI SnaBI SpeI
SphI Sse8387I SspBI SspI SwaI
TfiI Tsp509I Tth111I XbaI XcmI
XhoI XmnI
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# ONE CUT ENZYMES ( 44 enzymes) #
###################################
AccII 93 CGCG CG^CG
AgeI 38 ACCGGT A^CCGGT
ApaI 758 GGGCCC GGGCC^C
AvaI 1055 CCCGGG C^[CT]CG[GA]G
BamHI 1019 GGATCC G^GATCC
BbvCI 859 GCTGAGG CCTCAGC(-5/-2)
BfaI 799 CTAG C^TAG
BglII 416 AGATCT A^GATCT
BsgI 1027 CTGCAC GTGCAG(16/14)
BsoBI 1055 CCCGGG C^[CT]CG[GA]G
BspHI 635 TCATGA T^CATGA
BsrBI 147 CCGCTC CCGCTC(-3/-3)
BstEII 843 GGTCACC G^GT[ACGT]ACC
BstPI 843 GGTCACC G^GT[ACGT]ACC
BstUI 93 CGCG CG^CG
BstXI 737 CCAAGATCATGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
DrdI 487 GACTTTAGAGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EagI 79 CGGCCG C^GGCCG
EclXI 79 CGGCCG C^GGCCG
Eco52I 79 CGGCCG C^GGCCG
EcoNI 969 CCTGAGGGAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
EcoO65I 843 GGTCACC G^GT[ACGT]ACC
HincII 696 GTTGAC GT[CT]^[GA]AC
HindII 696 GTTGAC GT[CT]^[GA]AC
MaeI 799 CTAG C^TAG
MaeII 900 ACGT A^CGT
MaeIII 844 GTCAC ^GT[ACGT]AC
MseI 1169 TTAA T^TAA
MslI 834 CACCACAGTG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
MspA1I 592 CAGCTG C[AC]G^C[GT]G
MvnI 93 CGCG CG^CG
NcoI 546 CCATGG C^CATGG
PinAI 38 ACCGGT A^CCGGT
PvuII 592 CAGCTG CAG^CTG
RcaI 635 TCATGA T^CATGA
SapI 630 GCTCTTC GCTCTTC(1/4)
ScaI 806 AGTACT AGT^ACT
SmaI 1055 CCCGGG CCC^GGG
StuI 666 AGGCCT AGG^CCT
Tru9I 1169 TTAA T^TAA
Tsp45I 844 GTCAC ^GT[GC]AC
TspRI 839 CAGTG CA[GC]TG[ACGT][ACGT]^
XmaCI 1055 CCCGGG C^CCGGG
XmaI 1055 CCCGGG C^CCGGG
###################################
# TWO CUT ENZYMES ( 35 enzymes) #
###################################
AcyI 60 GGCGCC G[GA]^CG[CT]C
73 GGCGCC
AfaI 807 GTAC GT^AC
902 GTAC
AhdI 359 GACCCCAGGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
899 GACGTACAGTC
AlwNI 110 CAGGCGCTG CAG[ACGT][ACGT][ACGT]^CTG
369 CAGAGACTG
AocI 405 CCTGAGG CC^T[ACGT]AGG
969 CCTGAGG
AspEI 359 GACCCCAGGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
899 GACGTACAGTC
AspHI 340 GAGCAC G[AT]GC[AT]^C
831 GAGCAC
BalI 188 TGGCCA TGG^CCA
515 TGGCCA
BanI 60 GGCGCC G^G[CT][GA]CC
73 GGCGCC
BbeI 60 GGCGCC GGCGC^C
73 GGCGCC
BsaHI 60 GGCGCC G[GA]^CG[CT]C
73 GGCGCC
BsaWI 38 ACCGGT [AT]^CCGG[AT]
314 ACCGGA
BsiEI 79 CGGCCG CG[GA][CT]^CG
264 CGACCG
BsiHKAI 340 GAGCAC G[AT]GC[AT]^C
831 GAGCAC
BsmBI 877 GAGACG CGTCTC(1/5)
897 GAGACG
BsrFI 38 ACCGGT [GA]^CCGG[CT]
81 GCCGGT
Bsu36I 405 CCTGAGG CC^T[ACGT]AGG
969 CCTGAGG
Cfr10I 38 ACCGGT [GA]^CCGG[CT]
81 GCCGGT
DsaI 546 CCATGG C^C[GA][CT]GG
701 CCGTGG
Eam1105I 359 GACCCCAGGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
899 GACGTACAGTC
EarI 631 CTCTTC CTCTTC(1/4)
652 GAAGAG
Eco81I 405 CCTGAGG CC^T[ACGT]AGG
969 CCTGAGG
HgaI 882 GACGC GACGC(5/10)
101 GCGTC
Hin1I 60 GGCGCC G[GA]^CG[CT]C
73 GGCGCC
KasI 60 GGCGCC G^GCGCC
73 GGCGCC
Ksp632I 631 CTCTTC CTCTTC(1/4)
652 GAAGAG
MluNI 188 TGGCCA TGG^CCA
515 TGGCCA
MscI 188 TGGCCA TGG^CCA
515 TGGCCA
NarI 60 GGCGCC GG^CGCC
73 GGCGCC
PflMI 525 CCAGTCTGTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
909 CCAGTCCTTGG
PstI 454 CTGCAG CTGCA^G
589 CTGCAG
RsaI 807 GTAC GT^AC
902 GTAC
SfoI 60 GGCGCC GGC^GCC
73 GGCGCC
SmlI 616 CTCAAG C^T[CT][GA]AG
1033 CTTGAG
Van91I 525 CCAGTCTGTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
909 CCAGTCCTTGG
#########################################
# 3 OR MORE CUT ENZYMES ( 67 enzymes) #
#########################################
AciI AluI AlwI AvaII BanII
BbvI BcnI BmyI BpmI BsaI
BsaJI BseRI BsiYI BslI BsmAI
BsmFI Bsp1286I BsrI BssKI BstF5I
BstNI BstYI Cac8I CfoI Cfr13I
DdeI DpnII DraII EaeI EcoO109I
EcoRII EcoT14I FauI Fnu4HI FokI
HaeII HaeIII HapII HhaI HinP1I
HinfI HpaII HphI ItaI MboI
MboII MflI MnlI MspI MvaI
MwoI NciI NdeII NlaIII NlaIV
PleI PpuMI Sau3AI Sau96I ScrFI
SfaNI SfcI StyI TaqI TseI
TthHB8I XhoII