(Circular) (Six-base) MAPSORT of: pFN21AB6602.seq Check: 6040 from: 1 to: 5995 pFN21AB6602 5995 bp With 182 enzymes: SgfI * October 20, 2009 10:57 .. AatII G_ACGT'C Cuts at: 278 331 414 600 1536 3805 278 Size: 53 83 186 936 2269 2468 Fragments arranged by size: 2468 2269 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 3338 1937 Size: 1401 4594 Acc65I G'GTAC_C Cuts at: 3316 3316 Size: 5995 AclI AA'CG_TT Cuts at: 1563 4122 4495 1563 Size: 2559 373 3063 Fragments arranged by size: 3063 2559 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 2149 2972 4058 5442 5710 1632 Size: 517 823 1086 1384 268 1917 Fragments arranged by size: 1917 1384 1086 823 517 268 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 5798 AflIII A'CryG_T Cuts at: 1203 4894 5871 1203 Size: 3691 977 1327 Fragments arranged by size: 3691 1327 977 AgeI A'CCGG_T Cuts at: 2043 4826 2043 Size: 2783 3212 AhdI GACnn_n'nnGTC Cuts at: 2369 2909 4724 2369 Size: 540 1815 3640 Fragments arranged by size: 3640 1815 540 AleI CACnn'nnGTG Cuts at: 1166 2843 1166 Size: 1677 4318 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 2474 3224 1184 Size: 1290 750 3955 Fragments arranged by size: 3955 1290 750 AlwNI CAG_nnn'CTG Cuts at: 2120 2379 3738 5310 2120 Size: 259 1359 1572 2805 Fragments arranged by size: 2805 1572 1359 259 ApaI G_GGCC'C Cuts at: 2767 2767 Size: 5995 ApaLI G'TGCA_C Cuts at: 4052 5208 4052 Size: 1156 4839 ApoI r'AATT_y Cuts at: 1493 3304 3530 3682 5791 1493 Size: 1811 226 152 2109 1697 Fragments arranged by size: 2109 1811 1697 226 152 AseI AT'TA_AT Cuts at: 160 1032 4549 160 Size: 872 3517 1606 Fragments arranged by size: 3517 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 5995 AvaI C'yCGr_G Cuts at: 1870 1943 3060 3320 1870 Size: 73 1117 260 4545 Fragments arranged by size: 4545 1117 260 73 BamHI G'GATC_C Cuts at: 3024 3325 3692 3024 Size: 301 367 5327 Fragments arranged by size: 5327 367 301 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2065 2078 3316 4771 618 Size: 325 217 627 278 13 1238 1455 1842 Fragments arranged by size: 1842 1455 1238 627 325 278 217 13 BanII G_rGCy'C Cuts at: 1391 2057 2129 2419 2559 2767 3314 1391 Size: 666 72 290 140 208 547 4072 Fragments arranged by size: 4072 666 547 290 208 140 72 BbeI G_GCGC'C Cuts at: 2069 2082 2069 Size: 13 5982 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 928 Size: 971 5024 BbvCI CC'TCA_GC Cuts at: 2865 2865 Size: 5995 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 4186 4220 1903 Size: 34 2249 34 3678 Fragments arranged by size: 3678 2249 34 34 BciVI GTATCCnnnnn_n' Cuts at: 3887 5097 3887 Size: 1210 4785 BclI T'GATC_A Cuts at: 1548 1548 Size: 5995 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 4606 136 Size: 107 122 71 631 605 2934 1525 Fragments arranged by size: 2934 1525 631 605 122 107 71 BglII A'GATC_T Cuts at: 2421 5990 2421 Size: 3569 2426 BlpI GC'TnA_GC Cuts at: 3412 3412 Size: 5995 Bme1580I G_kGCm'C Cuts at: 1790 2767 4056 5212 1790 Size: 977 1289 1156 2573 Fragments arranged by size: 2573 1289 1156 977 BmgBI CAC'GTC Cuts at: 1318 1318 Size: 5995 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 3726 4684 442 Size: 416 53 83 399 24 2309 958 1753 Fragments arranged by size: 2309 1753 958 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 5995 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2327 2348 2401 2621 3143 4655 1239 Size: 1088 21 53 220 522 1512 2579 Fragments arranged by size: 2579 1512 1088 522 220 53 21 Bpu10I CC'TnA_GC Cuts at: 2865 2865 Size: 5995 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 2606 3059 4120 4985 5283 2606 Size: 453 1061 865 298 3318 Fragments arranged by size: 3318 1061 865 453 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 2243 2303 2823 3260 4658 882 Size: 617 744 60 520 437 1398 2219 Fragments arranged by size: 2219 1398 744 617 520 437 60 BsaAI yAC'GTr Cuts at: 493 1204 3786 493 Size: 711 2582 2702 Fragments arranged by size: 2702 2582 711 BsaBI GATnn'nnATC Cuts at: 5989 5989 Size: 5995 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 2066 2079 3802 4184 Size: 53 83 186 936 533 13 1723 382 Cuts at: 4184 4819 275 Size: 635 1451 Fragments arranged by size: 1723 1451 936 635 533 382 186 83 53 13 BsaWI w'CCGG_w Cuts at: 2043 2319 4428 4826 5100 5247 5658 2043 Size: 276 2109 398 274 147 411 2380 Fragments arranged by size: 2380 2109 411 398 276 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 2921 2951 1178 Size: 30 165 30 1518 30 4222 Fragments arranged by size: 4222 1518 165 30 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2314 2642 2813 2846 1186 Size: 1128 328 171 33 4335 Fragments arranged by size: 4335 1128 328 171 33 BseYI C'CCAG_C Cuts at: 2069 2120 5198 5694 2069 Size: 51 3078 496 2370 Fragments arranged by size: 3078 2370 496 51 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 3017 1163 Size: 573 1281 4141 Fragments arranged by size: 4141 1281 573 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 2087 2272 3445 4206 5234 1540 Size: 275 186 86 185 1173 761 1028 2301 Fragments arranged by size: 2301 1173 1028 761 275 186 185 86 BsiHKAI G_wGCw'C Cuts at: 2349 2840 4056 4141 5212 2349 Size: 491 1216 85 1071 3132 Fragments arranged by size: 3132 1216 1071 491 85 BsmI GAATG_Cn' Cuts at: 3506 3599 3506 Size: 93 5902 BsmBI CGTCTCn'nnnn_ Cuts at: 2876 2896 2876 Size: 20 5975 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2057 2129 2349 2419 2559 2767 2840 Size: 399 267 72 220 70 140 208 73 Cuts at: 2840 3314 4056 4141 5212 1391 Size: 474 742 85 1071 2174 Fragments arranged by size: 2174 1071 742 474 399 267 220 208 140 85 73 72 70 BspEI T'CCGG_A Cuts at: 5658 5658 Size: 5995 BspHI T'CATG_A Cuts at: 2640 3884 5614 2640 Size: 1244 1730 3021 Fragments arranged by size: 3021 1730 1244 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 3350 844 Size: 2506 3489 BsrBI CCG'CTC Cuts at: 1394 1823 2154 3882 1394 Size: 429 331 1728 3507 Fragments arranged by size: 3507 1728 429 331 BsrDI GCAATG_nn' Cuts at: 65 1248 4490 4664 65 Size: 1183 3242 174 1396 Fragments arranged by size: 3242 1396 1183 174 BsrFI r'CCGG_y Cuts at: 1700 1810 2043 2086 4639 4826 1700 Size: 110 233 43 2553 187 2869 Fragments arranged by size: 2869 2553 233 187 110 43 BsrGI T'GTAC_A Cuts at: 96 96 Size: 5995 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 5995 BssSI C'ACGA_G Cuts at: 4055 5067 4055 Size: 1012 4983 BstAPI GCAn_nnn'nTGC Cuts at: 794 794 Size: 5995 BstBI TT'CG_AA Cuts at: 4799 4799 Size: 5995 BstEII G'GTnAC_C Cuts at: 1184 2848 1184 Size: 1664 4331 BstXI CCAn_nnnn'nTGG Cuts at: 2749 2749 Size: 5995 BstYI r'GATC_y Cuts at: 1972 2139 2421 3024 3325 3692 4077 4094 5546 Size: 167 282 603 301 367 385 17 1452 Cuts at: 5546 5632 5644 5990 1972 Size: 86 12 346 1977 Fragments arranged by size: 1977 1452 603 385 367 346 301 282 167 86 17 12 Bsu36I CC'TnA_GG Cuts at: 2411 2975 2411 Size: 564 5431 BtgI C'CryG_G Cuts at: 513 1065 2551 2706 513 Size: 552 1486 155 3802 Fragments arranged by size: 3802 1486 552 155 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 487 Size: 683 56 5256 Fragments arranged by size: 5256 683 56 BtsI GCAGTG_nn' Cuts at: 3511 4305 4325 3511 Size: 794 20 5181 Fragments arranged by size: 5181 794 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 5995 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 2418 2453 529 Size: 35 1854 35 4071 Fragments arranged by size: 4071 1854 35 35 DraI TTT'AAA Cuts at: 3299 3299 Size: 5995 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 2498 5002 809 Size: 507 1182 2504 1802 Fragments arranged by size: 2504 1802 1182 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2084 2100 2193 Size: 54 1425 50 275 18 254 16 93 Cuts at: 2193 2520 3442 4331 5960 8 Size: 327 922 889 1629 43 Fragments arranged by size: 1629 1425 922 889 327 275 254 93 54 50 43 18 16 EagI C'GGCC_G Cuts at: 1537 1812 2084 3442 1537 Size: 275 272 1358 4090 Fragments arranged by size: 4090 1358 275 272 EarI CTCTTCn'nnn_ Cuts at: 1353 2642 2652 3925 1353 Size: 1289 10 1273 3423 Fragments arranged by size: 3423 1289 1273 10 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 4582 4956 5102 387 Size: 1064 138 2993 374 146 1280 Fragments arranged by size: 2993 1280 1064 374 146 138 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2149 2327 2348 2401 2621 2972 3143 Size: 393 517 178 21 53 220 351 171 Cuts at: 3143 4058 4655 5442 5710 1239 Size: 915 597 787 268 1524 Fragments arranged by size: 1524 915 787 597 517 393 351 268 220 178 171 53 21 EcoNI CCTnn'n_nnAGG Cuts at: 2978 2978 Size: 5995 EcoO109I rG'GnC_Cy Cuts at: 2188 2363 2407 2734 3097 2188 Size: 175 44 327 363 5086 Fragments arranged by size: 5086 363 327 175 44 EcoRI G'AATT_C Cuts at: 3304 3682 3304 Size: 378 5617 FalI AAGnnnnnCTTnnnnnnnn_nnnnn' Cuts at: 2977 2977 Size: 5995 FspI TGC'GCA Cuts at: 4501 4501 Size: 5995 HaeII r_GCGC'y Cuts at: 1678 2069 2082 2121 3079 5142 1678 Size: 391 13 39 958 2063 2531 Fragments arranged by size: 2531 2063 958 391 39 13 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2355 2387 2602 2634 2895 Size: 32 135 32 783 32 215 32 261 Cuts at: 2895 2921 2927 2953 4636 4668 4710 4742 1373 Size: 26 6 26 1683 32 42 32 2626 Fragments arranged by size: 2626 1683 783 261 215 135 42 32 32 32 32 32 32 26 26 6 HincII GTy'rAC Cuts at: 669 1657 1938 2703 3339 3585 669 Size: 988 281 765 636 246 3079 Fragments arranged by size: 3079 988 765 636 281 246 HindIII A'AGCT_T Cuts at: 748 748 Size: 5995 HpaI GTT'AAC Cuts at: 3585 3585 Size: 5995 KasI G'GCGC_C Cuts at: 2065 2078 2065 Size: 13 5982 KpnI G_GTAC'C Cuts at: 3320 3320 Size: 5995 MfeI C'AATT_G Cuts at: 3594 3594 Size: 5995 MluI A'CGCG_T Cuts at: 5871 5871 Size: 5995 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 2851 5109 5293 1305 Size: 1546 2258 184 2007 Fragments arranged by size: 2258 2007 1546 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 2195 2522 10 Size: 54 1425 343 363 327 3483 Fragments arranged by size: 3483 1425 363 343 327 54 MslI CAynn'nnrTG Cuts at: 518 1166 2843 3953 4312 4471 518 Size: 648 1677 1110 359 159 2042 Fragments arranged by size: 2042 1677 1110 648 359 159 MspA1I CmG'CkG Cuts at: 1606 2599 3411 4088 5236 5481 1606 Size: 993 812 677 1148 245 2120 Fragments arranged by size: 2120 1148 993 812 677 245 NarI GG'CG_CC Cuts at: 2066 2079 2066 Size: 13 5982 NcoI C'CATG_G Cuts at: 513 1065 2551 513 Size: 552 1486 3957 Fragments arranged by size: 3957 1486 552 NdeI CA'TA_TG Cuts at: 387 387 Size: 5995 NheI G'CTAG_C Cuts at: 1052 1052 Size: 5995 NotI GC'GGCC_GC Cuts at: 3442 3442 Size: 5995 NspI r_CATG'y Cuts at: 1132 1229 3296 3353 4898 1132 Size: 97 2067 57 1545 2229 Fragments arranged by size: 2229 2067 1545 97 57 PasI CC'CwG_GG Cuts at: 1348 2050 1348 Size: 702 5293 PciI A'CATG_T Cuts at: 4894 4894 Size: 5995 PflMI CCAn_nnn'nTGG Cuts at: 1108 2536 2920 1108 Size: 1428 384 4183 Fragments arranged by size: 4183 1428 384 PfoI T'CCnGG_A Cuts at: 2135 2340 2135 Size: 205 5790 PmeI GTTT'AAAC Cuts at: 3299 3299 Size: 5995 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 4064 4096 5577 5609 4064 Size: 32 1481 32 4450 Fragments arranged by size: 4450 1481 32 32 PpuMI rG'GwC_Cy Cuts at: 2363 2407 2734 2363 Size: 44 327 5624 Fragments arranged by size: 5624 327 44 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 5995 PsiI TTA'TAA Cuts at: 3565 3565 Size: 5995 PspOMI G'GGCC_C Cuts at: 2763 2763 Size: 5995 PstI C_TGCA'G Cuts at: 830 2463 2598 3347 830 Size: 1633 135 749 3478 Fragments arranged by size: 3478 1633 749 135 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 5995 PvuII CAG'CTG Cuts at: 2599 2599 Size: 5995 SalI G'TCGA_C Cuts at: 1936 3337 1936 Size: 1401 4594 SanDI GG'GwC_CC Cuts at: 2363 2363 Size: 5995 SapI GCTCTTCn'nnn_ Cuts at: 2642 2642 Size: 5995 SbfI CC_TGCA'GG Cuts at: 3347 3347 Size: 5995 ScaI AGT'ACT Cuts at: 2813 4243 5955 2813 Size: 1430 1712 2853 Fragments arranged by size: 2853 1712 1430 SfcI C'TryA_G Cuts at: 826 1045 2459 2594 3002 3343 4478 5159 5350 Size: 219 1414 135 408 341 1135 681 191 Cuts at: 5350 826 Size: 1471 Fragments arranged by size: 1471 1414 1135 681 408 341 219 191 135 SfoI GGC'GCC Cuts at: 2067 2080 2067 Size: 13 5982 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 5995 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 5995 SmaI CCC'GGG Cuts at: 1872 3062 3322 1872 Size: 1190 260 4545 Fragments arranged by size: 4545 1190 260 SmlI C'TyrA_G Cuts at: 820 1017 1943 2621 3038 4099 5000 5262 820 Size: 197 926 678 417 1061 901 262 1553 Fragments arranged by size: 1553 1061 926 901 678 417 262 197 SnaBI TAC'GTA Cuts at: 493 3786 493 Size: 3293 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 5995 SphI G_CATG'C Cuts at: 1132 3353 1132 Size: 2221 3774 SspI AAT'ATT Cuts at: 5 52 3919 5 Size: 47 3867 2081 Fragments arranged by size: 3867 2081 47 StuI AGG'CCT Cuts at: 1517 2673 1517 Size: 1156 4839 StyI C'CwwG_G Cuts at: 513 1065 2551 2919 3189 3434 513 Size: 552 1486 368 270 245 3074 Fragments arranged by size: 3074 1486 552 368 270 245 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 2327 4022 4039 4192 4377 4822 325 Size: 1255 747 1695 17 153 185 445 1498 Fragments arranged by size: 1695 1498 1255 747 445 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 2811 4241 5953 Size: 274 80 33 51 1443 834 1430 1712 Cuts at: 5953 96 Size: 138 Fragments arranged by size: 1712 1443 1430 834 274 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 5955 5987 5955 Size: 32 5963 Tth111I GACn'n_nGTC Cuts at: 1316 1316 Size: 5995 XbaI T'CTAG_A Cuts at: 3331 3331 Size: 5995 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 5995 XmaI C'CCGG_G Cuts at: 1870 3060 3320 1870 Size: 1190 260 4545 Fragments arranged by size: 4545 1190 260 XmnI GAAnn'nnTTC Cuts at: 4124 4124 Size: 5995 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 3803 276 Size: 53 83 186 936 2269 2468 Fragments arranged by size: 2468 2269 936 186 83 53 Enzymes that do cut: AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbeI BbsI BbvCI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI BsmBI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI Bsu36I BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI EarI EciI Eco57MI EcoNI EcoO109I EcoRI FalI FspI HaeII Hin4I HincII HindIII HpaI KasI KpnI MfeI MluI MmeI MscI MslI MspA1I NarI NcoI NdeI NheI NotI NspI PasI PciI PflMI PfoI PmeI PpiI PpuMI PshAI PsiI PspOMI PstI PvuI PvuII SalI SanDI SapI SbfI ScaI SfcI SfoI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XhoI XmaI XmnI ZraI Enzymes that do not cut: AarI AfeI AloI AscI AvrII BaeI BfrBI BplI BsiWI BstZ17I DraIII EcoICRI EcoRV FseI FspAI NaeI NgoMIV NruI NsiI PacI PmlI PspXI PsrI RsrII SacI SacII SexAI SfiI SrfI SwaI XcmI