################################## # NO CUT ENZYMES ( 164 enzymes) # ################################## AatII AccI AccII AccIII AciI AclI AcyI AgeI AhdI Alw44I AocI Aor51HI ApaI ApaLI AscI AseI AsnI Asp700I AspEI AspHI AspI AvaI AviII AvrII BalI BamHI BanII BbeI BbrPI BbvCI BcgI BciVI BglI BlnI BlpI BmyI Bpu1102I BsaAI BsaBI BsaHI BsaI BseAI BseRI BsiEI BsiHKAI BsiWI BsiYI BslI BsmI BsoBI Bsp1286I Bsp1407I BspDI BspEI BspMI BsrBI BsrFI BsrGI BssHII Bst1107I BstAPI BstEII BstPI BstUI BstZ17I Bsu36I CelII CfoI Cfr10I ClaI CpoI DraI DraII DraIII DrdI DsaI EaeI EagI Eam1105I EarI EclXI Eco47III Eco52I Eco81I EcoNI EcoO109I EcoO65I EcoRV EcoT22I FauI FseI FspI HaeII HaeIII HhaI Hin1I HinP1I HincII HindII HpaI KasI Ksp632I KspI MamI MfeI MluI MluNI MroI MscI MspA1I MunI MvnI NaeI NarI NcoI NdeI NgoMIV NheI NotI NruI NsiI PacI PaeR7I PflFI PflMI PinAI PmaCI PmeI PmlI PpuMI PshAI PshBI Psp1406I PstI PvuI PvuII RsrII SacI SacII SalI SapI SbfI ScaI SfcI SfiI SfoI SgrAI SmaI SnaBI SpeI SphI Sse8387I SspBI SspI StuI SwaI TfiI Tth111I Van91I XbaI XhoI XmaCI XmaI XmnI ################################### # ONE CUT ENZYMES ( 29 enzymes) # ################################### AflII 774 CTTAAG C^TTAAG AflIII 241 ACATGT A^C[GA][CT]GT AlwNI 641 CAGGTTCTG CAG[ACGT][ACGT][ACGT]^CTG BclI 800 TGATCA T^GATCA BcnI 56 CCCGG CC^[GC]GG BfaI 293 CTAG C^TAG BfrI 774 CTTAAG C^TTAAG BglII 109 AGATCT A^GATCT BsaWI 380 ACCGGA [AT]^CCGG[AT] BsgI 8 CTGCAC GTGCAG(16/14) BsmBI 222 GAGACG CGTCTC(1/5) BspHI 350 TCATGA T^CATGA BspLU11I 241 ACATGT A^CATGT BsrDI 141 GCAATG GCAATG(2/0) BssSI 611 CTCGTG CACGAG(-5/-1) BstBI 19 TTCGAA TT^CGAA BstXI 78 CCAAGGATCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG FbaI 800 TGATCA T^GATCA HphI 363 TCACC GGTGA(8/7) MaeI 293 CTAG C^TAG MslI 153 CATAAGTGTG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG NciI 56 CCCGG CC^[GC]GG NspV 19 TTCGAA TT^CGAA RcaI 350 TCATGA T^CATGA SexAI 639 ACCAGGT A^CC[AT]GGT SfaNI 655 GATGC GCATC(5/9) SfuI 19 TTCGAA TT^CGAA SmlI 774 CTTAAG C^T[CT][GA]AG XcmI 106 CCAAGATCTGAGTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG ################################### # TWO CUT ENZYMES ( 25 enzymes) # ################################### Acc65I 247 GGTACC G^GTACC 371 GGTACC AlwI 82 GGATC GGATC(4/5) 90 GGATC Asp718I 247 GGTACC G^GTACC 371 GGTACC AvaII 26 GGACC G^G[AT]CC 567 GGACC BanI 247 GGTACC G^G[CT][GA]CC 371 GGTACC BbsI 237 GAAGAC GAAGAC(2/6) 820 GTCTTC BpmI 734 CTCCAG CTGGAG(16/14) 40 CTGGAG BpuAI 237 GAAGAC GAAGAC(2/6) 820 GTCTTC BsmAI 222 GAGAC GTCTC(1/5) 214 GTCTC Cac8I 288 GCTTGC GC[ACGT]^[ACGT]GC 885 GCTTGC Cfr13I 26 GGACC G^G[ACGT]CC 567 GGACC EcoRI 456 GAATTC G^AATTC 623 GAATTC HapII 57 CCGG C^CGG 381 CCGG HgaI 70 GACGC GACGC(5/10) 918 GCGTC HinfI 313 GACTC G^A[ACGT]TC 498 GAGTC HpaII 57 CCGG C^CGG 381 CCGG KpnI 247 GGTACC GGTAC^C 371 GGTACC MaeII 225 ACGT A^CGT 450 ACGT MspI 57 CCGG C^CGG 381 CCGG MwoI 885 GCTTGCTTCGC GC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GC 987 GCAACAACAGC NspI 206 GCATGT [GA]CATG^[CT] 241 ACATGT PleI 313 GACTC GAGTC(4/5) 498 GAGTC Sau96I 26 GGACC G^G[ACGT]CC 567 GGACC TaqI 20 TCGA T^CGA 685 TCGA TthHB8I 20 TCGA T^CGA 685 TCGA ######################################### # 3 OR MORE CUT ENZYMES ( 40 enzymes) # ######################################### AcsI AfaI AluI ApoI BbvI BsaJI BsmFI BsrI BssKI BstF5I BstNI BstYI DdeI DpnII EcoRII EcoT14I Fnu4HI FokI HindIII ItaI MaeIII MboI MboII MflI MnlI MseI MvaI NdeII NlaIII NlaIV RsaI Sau3AI ScrFI StyI Tru9I TseI Tsp45I Tsp509I TspRI XhoII